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| Chinese leek (Allium tuberosum Rottler ex Sprengel) reduced disease symptom caused by root-knot nematode |
| HUANG Yong-hong, MAO Zhen-chuan, XIE Bing-yan |
1、College of Horticulture, Qingdao Agricultural University, Qingdao 266109, P.R.China
2、Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
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摘要 Root-knot nematodes (RKNs, Meloidogyne spp.) are obligate biotrophic parasites that settle close to the vascular tissues in plant roots. The diseases resulting from RKN infections cause serious damage to agricultural production worldwide. In the present paper, the resistance of Chinese leek (Allium tuberosum Rottler ex Sprengel) against RKNs, its suppressive effect on nematode disease, its nematicidal activity and its component profile were studied to identify an efficient disease control method. In soil heavily infected by nematodes, Chinese leek showed strong resistance to RKNs. Additionally, the gall indexes of cucumber plants rotated with Chinese leek and of tomato plants intercropped with Chinese leek were reduced by 70.2 and 41.1%, respectively. In a pot experiment, the gall indexes of Chinese leek extract-treated tomato and cucumber plants were reduced by 88.9 and 75.9%, respectively. In an in vitro experiment, the mortality rate of a RKN (Meloidogyne incognita J2) treated with Chinese leek extract was significantly higher than that of the control. The gas chromatography- mass spectrometry (GC-MS) analysis revealed that glycosides, carboxylic acids, ketones and organic sulfides are the main components in the Chinese leek extract. This study revealed that Chinese leek possesses a high resistance to RKNs, has strong nematicidal activity against M. incognita and can significantly reduce the incidence of disease caused by nematodes.
Abstract Root-knot nematodes (RKNs, Meloidogyne spp.) are obligate biotrophic parasites that settle close to the vascular tissues in plant roots. The diseases resulting from RKN infections cause serious damage to agricultural production worldwide. In the present paper, the resistance of Chinese leek (Allium tuberosum Rottler ex Sprengel) against RKNs, its suppressive effect on nematode disease, its nematicidal activity and its component profile were studied to identify an efficient disease control method. In soil heavily infected by nematodes, Chinese leek showed strong resistance to RKNs. Additionally, the gall indexes of cucumber plants rotated with Chinese leek and of tomato plants intercropped with Chinese leek were reduced by 70.2 and 41.1%, respectively. In a pot experiment, the gall indexes of Chinese leek extract-treated tomato and cucumber plants were reduced by 88.9 and 75.9%, respectively. In an in vitro experiment, the mortality rate of a RKN (Meloidogyne incognita J2) treated with Chinese leek extract was significantly higher than that of the control. The gas chromatography- mass spectrometry (GC-MS) analysis revealed that glycosides, carboxylic acids, ketones and organic sulfides are the main components in the Chinese leek extract. This study revealed that Chinese leek possesses a high resistance to RKNs, has strong nematicidal activity against M. incognita and can significantly reduce the incidence of disease caused by nematodes.
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Received: 21 January 2015
Accepted:
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| Fund: The research was supported by the National Natural Science Foundation of China (31272151, 31471864), the Agro-scientific Research in the Public Interest, China (201103018)and the China Agriculture Research System (CARS-25). |
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Corresponding Authors:
XIE Bing-yan, Tel: +86-10-82105979,E-mail: xiebingyan@caas.cn
E-mail: xiebingyan@caas.cn
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| About author: HUANG Yong-hong, E-mail: albertluke@126.com; |
Cite this article:
HUANG Yong-hong, MAO Zhen-chuan, XIE Bing-yan.
2016.
Chinese leek (Allium tuberosum Rottler ex Sprengel) reduced disease symptom caused by root-knot nematode. Journal of Integrative Agriculture, 15(2): 364-372.
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| Andrés M F, Gonzalez-Coloma A, Sanz J, Burillo J, SainzP. 2012. Nematicidal activity of essential oils: A review.Phytochemistry Reviews, 11, 371-390Bajaj K, Mahajan R. 1977. Phenolic compounds in tomatosusceptible and resistant to Meloidogyne incognita (Kofoidet White) Chitwood. Nematologia Mediterranea, 5, 329-333Balestra G M, Heydari A, Ceccarelli D, Ovidi E, Quattrucci A.2009. Antibacterial effect of Allium sativum and Ficus caricaextracts on tomato bacterial pathogens. Crop Protection,28, 807-811Browning M, Wallace D, Dawson C, Alm S, Amador J. 2006.Potential of butyric acid for control of soil-borne fungalpathogens and nematodes affecting strawberries. SoilBiology and Biochemistry, 38, 401-404Caboni P, Ntalli N G, Aissani N, Cavoski I, Angioni A. 2012.Nematicidal activity of (E,E)-2,4-decadienal and (E)-2-decenal from Ailanthus altissima against Meloidogynejavanica. Journal of Agricultural and Food Chemistry, 60,1146−1151.Chen C H, Chou T W, Cheng L H, Ho C W. 2011. In vitroanti-adenoviral activity of five Allium plants. Journal of theTaiwan Institute of Chemical Engineers, 42, 228−232.Chung D, Choi N, Maeng P J, Chun H K, Kim S. 2010.Purification and characterization of a novel fibrinolyticenzyme from chive (Allium tuberosum). Food Science andBiotechnology, 19, 697-702Dang Q L, Kim W K, Nguyen C M, Choi Y H, Choi G J, JangK S, Park M S, Lim C H, Lu N H, Kim J. 2011. Nematicidaland antifungal activities of annonaceous acetogenins fromAnnona squamosa against various plant pathogens. Journalof Agricultural and Food Chemistry, 59, 11160-11167Dang Q L, Lim C H, Kim J. 2012. Current status of botanicalpesticides for crop protection. Research in Plant Disease,18, 175-185Du S S, Zhang H M, Bai C Q, Wang C F, Liu Q Z, Liu Z L,Wang Y Y, Deng Z W. 2011. Nematocidal flavone-Cglycosidesagainst the root-knot nematode (Meloidogyneincognita) from Arisaema erubescens Tubers. Molecules,16, 5079-5086Echeverrigaray S, Zacaria J, Beltrão R. 2010. Nematicidalactivity of onoterpenoids against the root-knot nematodeMeloidogyne incognita. Phytopathology, 100, 199-203Ho W C, Wu T Y, Su H J, Ko W H. 2007. Effect of orientalmedicinal plant extracts on spore germination of Alternariabrassicicola and nature of inhibitory substances fromspeedweed. Plant Disease, 91, 1621-1624Huang Y H, Wang R C, Li C H, Zuo C W, Wei Y R, Zhang L, YiG J. 2012. Control of Fusarium wilt in banana with Chineseleek. European Journal of Plant Pathology, 134, 87-95Ibrahim H M M, Hosseini P, Alkharouf N W, Hussein E H A,Gamal El-Din A E K Y, Aly M A M, Matthews B F. 2011.Analysis of gene expression in soybean (Glycine max) roots in response to the root knot nematode Meloidogyneincognita using microarrays and KEGG pathways. BMCGenomics, 12, 220.Jaouannet M, Magliano M, Arguel M J, Gourgues M, EvangelistiE, Abad P, Rosso M N. 2013. The root-knot nematodecalreticulin Mi-CRT is a key effector in plant defensesuppression. Molecular Plant-Microbe Interactions, 26,97-105Lazarevic J S, Dor?evic A S, Zlatkovic B K, Radulovic N S,Palic R M. 2011. Chemical composition and antioxidantand antimicrobial activities of essential oil of Alliumsphaerocephalon L. subsp. sphaerocephalon (Liliaceae)inflorescences. Journal of the Science of Food andAgriculture, 91, 322-329Li H Q, Bai C Q, Chu S S, Zhou L, Du S S, Liu Z L, Liu Q Z. 2011.Chemical composition and toxicities of the essential oilderived from Kadsura heteroclita stems against Sitophiluszeamais and Meloidogyne incognita. Journal of MedicinalPlants Research, 5, 4943-4948Mao X, Li H, Chen X, Hu F. 2004. Extraction efficiency of soilnematodes by differnt methods. Chinese Journal of Ecology,23, 149-151Najjaa H, Neffati M, Zouari S, Ammar E. 2007. Essential oilcomposition and antibacterial activity of different extractsof Allium roseum L., a North African endemic species.Comptes Rendus Chimie, 10, 820-826Naz I, Palomares-Rius J E, Saifullah Blok V, Khan M R, Ali S,Ali S. 2013a. In vitro and in planta nematicidal activity ofFumaria parviflora (Fumariaceae) against the southern rootknotnematode Meloidogyne incognita. Plant Pathology,62, 943-952Naz I, Saifullah, Khan M R. 2013b. Nematicidal activity ofnonacosane-10-ol and 23a-Homostigmast-5-en-3β-olisolated from the roots of Fumaria parviflora (Fumariaceae).Journal of Agricultural and Food Chemistry, 61, 5689-5695Ntalli N G, Ferrari F, Giannakou I, Menkissoglu-SpiroudiU. 2010. Phytochemistry and nematicidal activity of theessential oils from 8 greek lamiaceae aromatic plants and13 terpene components. Journal of Agricultural and FoodChemistry, 58, 7856-7863Ntalli N G, Ferrari F, Giannakou I, Menkissoglu-Spiroudi U.2011a. Synergistic and antagonistic interactions of terpenesagainst Meloidogyne incognita and the nematicidal activityof essential oils from seven plants indigenous to Greece.Pest Management Science, 67, 341-351Ntalli N G, Manconi F, Leonti M, Maxia A, Caboni P. 2011b.Aliphatic ketones from Ruta chalepensis (Rutaceae) induceparalysis on root knot nematodes. Journal of Agriculturaland Food Chemistry, 59, 7098-7103Oka Y. 2012. Nematicidal activity of Verbesina encelioidesagainst the root-knot nematode Meloidogyne javanica andeffects on plant growth. Plant Soil, 355, 311-322Oka Y, Ben-Daniel B, Cohen Y. 2012. Nematicidal activity of theleaf powder and extracts of Myrtus communis against theroot-knot nematode Meloidogyne javanica. Plant Pathology,61, 1012-1020Oka Y, Nacar S, Putievsky E, Ravid U, Yaniv Z, Spiegel Y. 2000.Nematicidal activity of essential oils and their componentsagainst the root-knot nematode. Phytopathology, 90,710-715Pongsak R, Parichat P. 2009. Potential of Chinese chive oilas a natural antimicrobial for controlling Flavobacteriumcolumnare infection in Nile tilapia Oreochromis niloticus.Fisheries Science, 75, 1431-1437Powell N T, Melendez P L, Batten C K. 1971. Diseasecomplexes in tobacco involving Meloidogyne incognita andcertain soil-borne fungi. Phytopathology, 61, 1332-1337Rattanachaikunsopon P, Phumkhachorn P. 2009. Potential ofChinese chive oil as a natural antimicrobial for controllingFlavobacterium columnare infection in Nile tilapiaOreochromis niloticus. Fisheries Science, 75, 1431-1437Seo Y, Kim Y H. 2014. Control of meloidogyne incognita usingmixtures of organic acids. The Plant Pathology Journal,30, 450.Seyfi P, Mostafaie A, Mansouri K, Arshadi D, Mohammadi-Motlagh H R, Kiani A. 2010. In vitro and in vivo antiangiogenesiseffect of shallot (Allium ascalonicum): A heatstableand flavonoid-rich fraction of shallot extract potentlyinhibits angiogenesis. Toxicology In Vitro, 24, 1655-1661Shim S. 2012. Chelating effect of leek (Allium tuberosumRottler ex Sprengel) containing chlorophyll on Cd, Pb, andAs. Journal of the Korean Society for Applied BiologicalChemistry, 55, 311-315Tsao S M, Yin M C. 2001. In-vitro antimicrobial activity of fourdiallyl sulphides occurring narurelly in garlic and Chineseleek oil. Journal of Medical Microbiology, 50, 646-649Yin M C, Tsao S M. 1999. Inhibitory effect of seven Alliumplants upon three Aspergillus species. International Journalof Jood Microbiology, 49, 49-56Yu J Q. 1999. Allelopathic suppression of Pseudomonassolanacearum infection of tomato (Lycopersicon esculentum)in a tomato-Chinese chive (Allium tuberosum) intercroppingsystem. Journal of Chemical Ecology, 25, 2409-2417Zhang H, Mallik A, Zeng R S. 2013. Control of panama diseaseof banana by rotating and intercropping with Chinese chive(Allium tuberosum Rottler): Role of plant volatiles. Journalof Chemical Ecology, 39, 243-252 |
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