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Journal of Integrative Agriculture  2018, Vol. 17 Issue (2): 359-367    DOI: 10.1016/S2095-3119(17)61731-3
Special Issue: 线虫合辑Nematology
Horticulture Advanced Online Publication | Current Issue | Archive | Adv Search |
Comparison of rhizosphere and endophytic microbial communities of Chinese leek through high-throughput 16S rRNA gene Illumina sequencing
HUANG Yong-hong
College of Horticulture, Qingdao Agricultural University, Qingdao 266109, P.R.China
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Abstract  Chinese leek (Allium tuberosum Rottler ex Sprengel) is a common vegetable in China.  In our previous study, Chinese leek in rotation was found to have significant antifungal and nematicidal activity.  This study’s aim was to investigate the potential antifungal and nematicidal activity associated with rhizosphere or endophytic microbes of Chinese leek.  Thus, a total of 79 261 high-quality sequences were obtained from Chinese leek rhizosphere soil, leaf and root samples.  In the rhizosphere soil, the bacterial community comprised five dominant phyla: Proteobacteria (37.85%), Acidobacteria (10.99%), Bacteroidetes (8.24%), Cyanobacteria (7.79%) and Planctomycetes (7.1%).  The leaf and root bacterial communities comprised two dominant phyla: Cyanobacteria (83.42% in leaf and 75.44% in root) and Proteobacteria (14.75% in leaf and 21.04% in root).  Microbial diversity, richness and evenness in the rhizosphere soil bacterial community were higher than that in the endophytic bacterial communities.  The rhizosphere bacterial community was significantly different from the endophytic bacterial communities.  The endophytic bacterial communities from the leaf and the root were slightly, but not significantly different from each other.  This study’s findings would contribute to the isolation and identification of nematicidal and antifungal bacterial communities in Chinese leek.
Keywords:  Allium tuberosum Rottler ex Sprengel        rhizosphere soil        endophyte        bacterial community        Illumina sequencing  
Received: 28 February 2017   Accepted:

This work was supported by the National Natural Science Foundation of China (31471864 and 31272151) and the Qingdao Agricultural University High-level Personnel Start-up Fund, China (6631115024). We thank Beijing Ori-Gene Science and Technology (China) for providing the Illumina Miseq platform.

Corresponding Authors:  Correspondence HUANG Yong-hong, Tel: +86-532-88030910, E-mail:   

Cite this article: 

HUANG Yong-hong. 2018. Comparison of rhizosphere and endophytic microbial communities of Chinese leek through high-throughput 16S rRNA gene Illumina sequencing. Journal of Integrative Agriculture, 17(2): 359-367.

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