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Journal of Integrative Agriculture  2019, Vol. 18 Issue (3): 599-607    DOI: 10.1016/S2095-3119(18)62089-1
Special Issue: 植物细菌真菌合辑Plant Bacteria/Fungus
Plant Protection Advanced Online Publication | Current Issue | Archive | Adv Search |
Soil application of Trichoderma asperellum GDFS1009 granules promotes growth and resistance to Fusarium graminearum in maize
HE An-le1, LIU Jia1, WANG Xin-hua1, ZHANG Quan-guo2, SONG Wei2, CHEN Jie1 
1 School of Agriculture and Biology, Shanghai Jiao Tong University/State Key Laboratory of Microbial Metabolites/Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai 200240, P.R.China
2 Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031, P.R.China
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Of diseases affecting maize (Zea mays), Fusarium graminearum is one of the most common pathogenic fungi that cause stalk rot.  In the present study, the Trichoderma asperellum GDFS1009 strain was shown to be an effective biocontrol agent against stalk rot.  In a confrontation culture test, Trichoderma strain displayed an approximately 60% inhibition rate on the mycelial growth of F. graminearum.  In pot trials, the application of 2 g/pot of T. asperellum GDFS1009 granules had the best control effect on stalk rot at the seedling stage (up to 53.7%), while the average plant height and fresh weight were also significantly improved.  Additionally when fertilizer was added at 8 g/pot, the application of 3 g/pot of Trichoderma granules had the best control effect on maize stalk rot (40.95%).  In field trials, when inoculating F. graminearum alone, the disease index for inoculating was 62.45, but only 31.43 after treatment with T. asperellum GDFS1009 granules, suggesting a control efficiency of 49.67%.  Furthermore, in a naturally F. graminearum-infected field, Trichoderma granules, when applied for 3 consecutive years, showed significant control of stalk rot and increased yields.
Keywords:  Trichoderma asperellum        maize       Fusarium graminearum        stalk rot        biocontrol agent  
Received: 23 June 2018   Accepted:
Fund: This work was supported by the grants from the National Key Research and Development Program of China (2017YFD0200403, 2017YFD0201108, and 2017YFE0104900), the “948” Project of China (2016-X48), the National Natural Science Foundation of China (31750110455, 31872015), and the earmarked fund for China Agricultural Research System (CARS-02-26).
Corresponding Authors:  Correspondence CHEN Jie, Tel: +86-21-34206141, Fax: +86-21-64193285, E-mail:   
About author:  HE An-le, E-mail:;

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HE An-le, LIU Jia, WANG Xin-hua, ZHANG Quan-guo, SONG Wei, CHEN Jie. 2019. Soil application of Trichoderma asperellum GDFS1009 granules promotes growth and resistance to Fusarium graminearum in maize. Journal of Integrative Agriculture, 18(3): 599-607.

Contreras-Cornejo H A, Macias-Rodriguez L, Cortes-Penagos C, Lopez-Bucio J. 2009. Trichoderma virens, a plant beneficial fungus, enhances biomass production and promotes lateral root growth through an auxin-dependent mechanism in Arabidopsis. Plant Physiology, 149, 1579–1592.
Elad Y. 1996. Mechanisms involved in the biological control of Botrytis cinerea incited diseases. European Journal of Plant Pathology, 102, 719–732.
Ferrigo D, Raiola A, Piccolo E, Scopel C, Causin R. 2014. Trichoderma harzianum T22 induces in maize systemic resistance against Fusarium verticillioides. Journal of Plant Pathology, 96, 133–142.
Freeman G H. 1985. Statistical inferenc based on ranks. Biometrics, 41, 342–343.
Harman G E. 2000. Myths and dogmas of biocontrol: Changes in perceptions derived from research on Trichoderma harzianum T-22. Plant Disease, 84, 377–393.
Harman G E, Howell C R, Viterbo A, Chet I, Lorito M. 2004. Trichoderma species - Opportunistic, avirulent plant symbionts. Nature Reviews Microbiology, 2, 43–56.
Hermosa R, Viterbo A, Chet I, Monte E. 2012. Plant-beneficial effects of Trichoderma and of its genes. Microbiology, 158, 17–25.
Jaworska M, Dluniewska J. 2007. The effect of manganese ions on development antagonism of Trichoderma isolates. Polish Journal of Environmental Studies, 16, 549–553.
John R P, Tyagi R D, Prévost D, Brar S K, Pouleur S, Surampalli R Y. 2010. Mycoparasitic Trichoderma viride as a biocontrol agent against Fusarium oxysporum f. sp. adzuki and Pythium arrhenomanes and as a growth promoter of soybean. Crop Protection, 29, 1452–1459.
Li Y, Sun R, Yu J, Saravanakumar K, Chen J. 2016. Antagonistic and biocontrol potential of Trichoderma asperellum ZJSX5003 against the maize stalk rot pathogen Fusarium graminearum. Indian Journal of Microbiology, 56, 318–327.
Maag D, Kandula D, Müller C, Mendoza A, Wratten S D, Stewart A, Rostás M. 2014. Trichoderma atroviride LU132 promotes plant growth but not induced systemic resistance to Plutella xylostella in oilseed rape. Biocontrol, 59, 241–252.
Matarese F, Sarrocco S, Gruber S, Seidl-Seiboth V, Vannacci G. 2012. Biocontrol of Fusarium head blight: Interactions between Trichoderma and mycotoxigenic Fusarium. Microbiology, 158, 98–106.
Saravanakumar K, Dou K, Lu Z, Wang X, Li Y, Chen J. 2018. Enhanced biocontrol activity of cellulase from Trichoderma harzianum against Fusarium graminearum through activation of defense-related genes in maize. Physiological and Molecular Plant Pathology, 103, 130–136.
Tian Y, Tan Y, Liu N, Yan Z, Liao Y, Chen J, de Saeger S, Yang H, Zhang Q, Wu A. 2016. Detoxification of deoxynivalenol via glycosylation represents novel insights on antagonistic activities of Trichoderma when confronted with Fusarium graminearum. Toxins, 8, 335.
Tripathi P, Singh P C, Mishra A, Chauhan P S, Dwivedi S, Bais R T, Tripathi R D. 2013. Trichoderma: A potential bioremediator for environmental clean up. Clean Technologies and Environmental Policy, 15, 541–550.
Troian R F, Steindorff A S, Ramada M H, Arruda W, Ulhoa C J. 2014. Mycoparasitism studies of Trichoderma harzianum against Sclerotinia sclerotiorum: Evaluation of antagonism and expression of cell wall-degrading enzymes genes. Biotechnology Letters, 36, 2095–2101.
Vinale F, Sivasithamparam K, Ghisalberti E, Marra R, Barbetti M J, Li H, Woo S L, Lorito M. 2008. A novel role for the Trichoderma-plant interaction Trichoderma secondary metabolites in the interactions with plants. Physiological and Molecular Plant Pathology, 72, 80–86.
Vinodkumar S, Indumathi T, Nakkeeran S. 2017. Trichoderma asperellum (NVTA2) as a potential antagonist for the management of stem rot in carnation under protected cultivation. Biological Control, 113, 58–64.
Zhang D D, Min Y H, Yuan H X, Liu C Y. 2010. Toxicity assay and field control effect of several chemicals against corn stalk rot. Journal of Henan Agricultural Sciences, 8, 90–92. (in Chinese)
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