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Journal of Integrative Agriculture
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Screening and evaluation of plant-derived attractants for Loxostege sticticalis adult management

Hongnian Li1,2*, Ertao Li2*, Aiguo Kang3, Kebin Li2, Lei Zhang2, Huanhuan Dong2, Zhimin Wang2, Yangyang Wang3, Byambasuren Mijidsuren4, Fei Hu1, Jiao Yin2#, Zhaojun Wei#

1 School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China

2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

3 Plant Protection Station of Kangbao County in Hebei Province, Kangbao 076650, China

4 Plant Protection Research Institute of Mongolia, Ulaanbaatar 210153, Mongolia

 Highlights 

l Linalool, cis-anethole, trans-2-hexenal, and 1-octen-3-ol are attractants for L. sticticalis.

l Four compounds mixed at a ratio of 5:1:1:10 are recommended for managing L. sticticalis.

l The lure equipment with mixtures can be integrated into the “push-pull” strategy.

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摘要  

草地螟(鳞翅目:螟蛾科)是亚欧地区农牧业生产中的重要迁飞性害虫。利用植物挥发性有机化合物(pVOCs)作为引诱剂进行害虫监测与防控,是一种环境友好且高效的防治策略。然而,目前针对草地螟的pVOCs防控研究仍较为有限。本研究采用动态顶空采样技术,收集了草地螟雌虫偏好产卵的三种寄主植物(灰绿藜、狗尾草和紫花苜蓿)释放的挥发性物质,通过气质联用技术(GC-MS)鉴定出55种化合物,其中16种化合物在0.001-100 µg µL-1浓度范围内可引起草地螟成虫的电生理反应。进一步的室内行为学实验证实,芳樟醇、顺式茴香醇、反式-2-己烯醛和1-辛烯-3-醇这4种生物活性化合物对草地螟具有显著引诱效果,特别是当以5:1:5:1025号配方)和5:1:1:1021号配方)比例复配时,对成虫的引诱作用最为显著。田间试验结果显示,装载21号配方的2号诱捕器表现出最佳的田间诱捕效果。本研究证实,基于pVOCs的引诱剂可有效应用于草地螟成虫的监测和防控,为开发新型植物源引诱剂提供了重要思路。



Abstract  

The Loxostege sticticalis (Lepidoptera: Pyralidae) is a major migratory pest of agriculture and animal husbandry in Asia and Europe. Utilizing plant volatile organic compounds (pVOCs) as attractants for monitoring and controlling pests is considered an environmentally friendly and effective method. However, limited knowledge exists regarding applying pVOCs to manage L. sticticalis. Here, volatile compounds released by Chenopodium album, Setaria viridis, and Medicago sativa, the three preferred oviposition plants for L. sticticalis females, were collected using dynamic headspace sampling techniques. A total of 55 distinct compounds were identified through gas chromatography-mass spectrometry (GC-MS), and 16 compounds in the concentration range from 0.001 to 100 µg µL-1 elicited consistently enhanced electrophysiological responses in both male and female L. sticticalis. Subsequently, the attraction potential of four bioactive compoundslinalool, cis-anethole, trans-2-hexenal, and 1-octen-3-olwere further confirmed by indoor behavioral bioassays. The blends of linalool, cis-anethole, trans-2-hexenal, and 1-octen-3-ol mixed at ratios of 5:1:5:10 (formulation No. 25) and 5:1:1:10 (formulation No. 21) were highly attractive to L. sticticalis adults. Field-trapping assays indicated that lure No. 2 baited with formulation 21 demonstrated superior efficacy in field trapping. These findings suggest that pVOC-based attractants can be effectively employed for monitoring and mass trapping L. sticticalis adults, providing insights into the development of botanical attractants.

Keywords:  Loxostege sticticalis       plant volatile              gas chromatography-mass spectrometry (GC-MS)              electrophysiological response              behavioral bioassay  
Online: 25 April 2025  
Fund: 

This work was supported by the National Key R&D Program of China (2024YFE0113000), the Natural Science Foundation of Ningxia province (2022AAC03241), and the China Postdoctoral Science Foundation (2024M753573).

About author:  Hongnian Li, E-mail: lihntomato@163.com; Ertao Li, E-mail: 18330247086@163.com; #Correspondence Jiao Yin, E-mail: jyin@ippcaas.cn; Zhaojun Wei, E-mail: zjwei@hfut.edu.cn *These authors contributed equally to this study.

Cite this article: 

Hongnian Li, Ertao Li, Aiguo Kang, Kebin Li, Lei Zhang, Huanhuan Dong, Zhimin Wang, Yangyang Wang, Byambasuren Mijidsuren, Fei Hu, Jiao Yin, Zhaojun Wei. 2025. Screening and evaluation of plant-derived attractants for Loxostege sticticalis adult management. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2025.04.034

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