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Association analysis of grain traits with SSR markers between Aegilops tauschii and hexaploid wheat (Triticum aestivum L.) |
ZHAO Jing-lan, WANG Hong-wei, ZHANG Xiao-cun, DU Xu-ye, LI An-fei, KONG Ling-rang |
1、State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, P.R.China
2、Taishan Polytechnic, Tai’an 271000, P.R.China |
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摘要 Seven important grain traits, including grain length (GL), grain width (GW), grain perimeter (GP), grain area (GA), grain length/width ratio (GLW), roundness (GR), and thousand-grain weight (TGW), were analyzed using a set of 139 simple sequence repeat (SSR) markers in 130 hexaploid wheat varieties and 193 Aegilops tauschii accessions worldwide. In total, 1 612 alleles in Ae. tauschii and 1 360 alleles in hexaploid wheat (Triticum aestivum L.) were detected throughout the D genome. 197 marker-trait associations in Ae. tauschii were identified with 58 different SSR loci in 3 environments, and the average phenotypic variation value (R2) ranged from 0.68 to 15.12%. In contrast, 208 marker-trait associations were identified in wheat with 66 different SSR markers in 4 environments and the average phenotypic R2 ranged from 0.90 to 19.92%. Further analysis indicated that there are 6 common SSR loci present in both Ae. tauschii and hexaploid wheat, which are significantly associated with the 5 investigated grain traits (i.e., GA, GP, GR, GL, and TGW) and in total, 16 alleles derived from the 6 aforementioned SSR loci were shared by Ae. tauschii and hexaploid wheat. These preliminary data suggest the existence of common alleles may explain the evolutionary process and the selection between Ae. tauschii and hexaploid wheat. Furthermore, the genetic differentiation of grain shape and thousand-grain weight were observed in the evolutionary developmental process from Ae. tauschii to hexaploid wheat.
Abstract Seven important grain traits, including grain length (GL), grain width (GW), grain perimeter (GP), grain area (GA), grain length/width ratio (GLW), roundness (GR), and thousand-grain weight (TGW), were analyzed using a set of 139 simple sequence repeat (SSR) markers in 130 hexaploid wheat varieties and 193 Aegilops tauschii accessions worldwide. In total, 1 612 alleles in Ae. tauschii and 1 360 alleles in hexaploid wheat (Triticum aestivum L.) were detected throughout the D genome. 197 marker-trait associations in Ae. tauschii were identified with 58 different SSR loci in 3 environments, and the average phenotypic variation value (R2) ranged from 0.68 to 15.12%. In contrast, 208 marker-trait associations were identified in wheat with 66 different SSR markers in 4 environments and the average phenotypic R2 ranged from 0.90 to 19.92%. Further analysis indicated that there are 6 common SSR loci present in both Ae. tauschii and hexaploid wheat, which are significantly associated with the 5 investigated grain traits (i.e., GA, GP, GR, GL, and TGW) and in total, 16 alleles derived from the 6 aforementioned SSR loci were shared by Ae. tauschii and hexaploid wheat. These preliminary data suggest the existence of common alleles may explain the evolutionary process and the selection between Ae. tauschii and hexaploid wheat. Furthermore, the genetic differentiation of grain shape and thousand-grain weight were observed in the evolutionary developmental process from Ae. tauschii to hexaploid wheat.
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Received: 24 October 2014
Accepted:
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Fund: We acknowledge financial supports by the National 973 Program of China (2014CB138100), the National Natural Science Foundation of China (31171553, 31471488 and 31200982), and the National High-Tech R&D Program of China (2011AA100102). |
Corresponding Authors:
KONG Ling-rang, Tel: +86-538-8249278,E-mail: lkong@sdau.edu.cn
E-mail: lkong@sdau.edu.cn
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About author: ZHAO Jing-lan, Tel: +86-538-8628134, E-mail: zhaojlan@sina.
com; |
Cite this article:
ZHAO Jing-lan, WANG Hong-wei, ZHANG Xiao-cun, DU Xu-ye, LI An-fei, KONG Ling-rang.
2015.
Association analysis of grain traits with SSR markers between Aegilops tauschii and hexaploid wheat (Triticum aestivum L.). Journal of Integrative Agriculture, 14(10): 1936-1948.
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Addington J, Cornblatt B A, Cadenhead K S, Cannon T D,McGlashan T H, Perkins D O, Heinssen R. 2011. At clinicalhigh risk for psychosis: Outcome for nonconverters. TheAmerican Journal of Psychiatry, 168, 800-805Breseghello F, Sorrells M E. 2006. Association mapping ofkernel size and milling quality in wheat (Triticum aestivumL.) cultivars. Genetics, 172, 1165-1177Breseghello F, Sorrells M E. 2007. QTL analysis of kernel sizeand shape in two hexaploid wheat mapping populations.Field Crops Research, 101, 172-179Bradbury P J, Zhang Z, Kroon D E, Casstevens T M,Ramdoss Y, Buckler E S. 2007. TASSEL: Software forassociation mapping of complex traits in diverse samples.Bioinformatics, 23, 2633-2635Dubcovsky J, Dvorak J. 2007. Genome plasticity a key factorin the success of polyploid wheat under domestication.Science, 316, 1862-1866Dvorak J, Luo M C, Yang Z L, Zhang H B. 1998. The structureof the Aegilops tauschii genepool and the evolution ofhexaploid wheat. Theoretical and Applied Genetics, 97,657-670Evanno G, Regnaut S, Goudet J. 2005. Detecting the numberof clusters of individuals using the software STRUCTURE:A simulation study. Molecular Ecology, 14, 2611-2620Genc Y, Oldach K, Verbyla A P, Lott G, Hassan M, Tester M,McDonald G K. 2010. Sodium exclusion QTL associatedwith improved seedling growth in hexaploid wheat undersalinity stress. Theoretical and Applied Genetics, 121,877-894Gegas V C, Nazari A, Griffiths S, Simmonds J, Fish L, Orford S,Sayers L, Doonan J H, Snape J W. 2010. A genetic framework for grain size and shape variation in wheat. The PlantCell, 22, 1046-1056Hardy O J, Vekemans X. 2002. SPAGeDi: a versatile computerprogram to analyse spatial genetic structure at the individualor population levels. Molecular Ecology Resourses, 2,618-620Hsam S L K, Kieffer R, Zeller F J. 2001. Significance of Aegilopstauschii glutenin genes on bread-making properties ofwheat. Cereal Chemistry, 78, 521-525Jiang Q, Hou J, Hao C, Wang L, Ge H, Dong Y, Zhang X. 2011.The wheat (T. aestivum) sucrose synthase 2 gene (TaSus2)active in endosperm development is associated with yieldtraits. Functional & Integrative Genomics, 11, 49-61Kumar N, Kulwal P L, Gaur A, Tyagi A K, Khurana J P, KhuranaP, Balyan H S, Gupta P K. 2006. QTL analysis for grainweight in common wheat. Euphytica, 151, 135-144Lagudah E S, Halloran G M. 1988. Phylogenetic relationshipsof Triticum tauschii the D genome donor to hexaploid wheat.Theoretical and Applied Genetics, 75, 592-598Lelley T, Stachel M, Grausgruber H, Vollmann J. 2000.Analysis of relationships between Aegilops tauschii andthe D genome of wheat utilizing microsatellites. Genome,43, 661-668Marza F, Bai G H, Carver B F, Zhou W C. 2006. Quantitativetrait loci for yield and related traits in the wheat populationNing7840×Clark. Theoretical and Applied Genetics, 112,688-698Matsuoka Y, Nishioka E, Kawahara T, Takumi S. 2009.Genealogical analysis of subspecies divergence andspikelet-shape diversification in central Eurasian wild wheatAegilops tauschii Coss. Plant Systematics and Evolution,279, 233-244Matsuoka Y, Takumi S, Kawahara T. 2007. Natural variationfor fertile triploid F1 hybrid formation in all hexaploid wheatspeciation. Theoretical and Applied Genetics, 115, 509-518Matsuoka Y, Takumi S, Kawahara T. 2008. Flowering timediversification and dispersal in central Eurasian wild wheatAegilops tauschii (Poaceae): Genealogical and ecologicalframework. PLoS One, 3, e3138.Mir R R, Kumar N, Jaiswal V, Girdharwal N, Prasad M, BalyanH S, Gupta P K. 2012. Genetic dissection of grain weightin bread wheat through quantitative trait locus interval andassociation mapping. Molecular Breeding, 29, 963-972Nezhad K Z, Weber W E, Röder M S, Sharma S, LohwasserU, Meyer R C, Saal B, Börner A. 2012. QTL analysisfor thousand-grain weight under terminal drought stressin bread wheat (Triticum aestivum L.). Euphytica, 186,127-138Neumann K, Kobiljsdki B, Deneiae S, Varshney R K, BörnerA. 2011. Genome-association mapping - a case study inbread wheat (Triticum aestivum L.). Molecular Breeding,27, 37-58Okamoto Y, Nguyen A T, Yoshioka M, Iehisa J C, Takumi S.2013. Identification of quantitative trait loci controlling grain size and shape in the D genome of synthetic hexaploidwheat lines. Breeding Science, 63, 423-429Okamoto Y, Kajimura T, Ikeda T M, Takumi S. 2012. Evidencefrom principal component analysis for improvement of grainshape and spikelet morphology-related traits after hexaploidwheat speciation. Genes Genetic Systems, 87, 299-310Pritchard J K, Stephens M, Donnelly P. 2000. Inference ofpopulation structure using multilocus genotype data.Genetics, 155, 945-959Reif J C, Gowda M, Maurer H P, Longin C F H, Korzun V,Ebmeyer E, Bothe R, Pietsch C, Würschum T. 2011.Association mapping for quality traits in soft winter wheat.Theoretical and Applied Genetics, 122, 961-970Ritland K. 1996. Estimators for pairwise relatedness andindividual inbreeding coefficients. Genetical Research, 67,175-186Saghai-Maroof M A, Soliman K M, Jorgensen R A, Allard RW. 1984. Ribosomal DNA spacer-length polymorphisms inbarley: Mendelian inheritance, chromosomal location, andpopulation dynamics. Proceedings of the National Academyof Sciences of the United States of America, 81, 8014-8018Sun X, Marza F, Ma H, Carver B F, Bai G. 2010. Mappingquantitative trait loci for quality factors in an inter-classcross of US and Chinese wheat. Theoretical and AppliedGenetics, 120, 1041-1051Su Z, Hao C, Wang L, Dong Y, Zhang X. 2011. Identification anddevelopment of a functional marker of TaGW2 associatedwith grain weight in bread wheat (Triticum aestivum L.).Theoretical and Applied Genetics, 122, 211-223Talbert L E, Smith L Y, Blake N K. 1998. More than one originof hexaploid wheat is indicated by sequence comparisonof low-copy DNA. Genome, 41, 402-407Takumi S, Naka Y, Morihiro H, Matsuoka Y. 2009a. Expressionof morphological and flowering time variation throughallopolyploidization: An empirical study with 27 wheatsynthetics and their parental Aegilops tauschii accessions.Plant Breeding, 128, 585-590Takumi S, Nishioka E, Morihiro H, Kawahara T, Matsuoka Y.2009b. Natural variation of morphological traits in wild wheatprogenitor Aegilops tauschii Coss. Breeding Science, 59,579-588Tsilo T J, Hareland G A, Simsek S, Chao S, Anderson J M.2010. Genome mapping of kernel characteristics in hardred spring wheat breeding lines. Theoretical and AppliedGenetics, 121, 717-730Vazquez M D, Peterson C J, Riera-Lizarazu O, Chen X,Heesacker A, Ammar K, Mundt C C. 2012. Genetic analysisof adult plant, quantitative resistance to stripe rust in wheatvariety ‘Stephens’ in multi-environment trials. Theoreticaland Applied Genetics, 124, 1-11Wang J, Luo M C, Chen Z, You F M, Wei Y, Zheng Y, DvorakJ. 2013. Aegilops tauschii single nucleotide polymorphismsshed light on the origins of wheat D-genome geneticdiversity and pinpoint the geographic origin of hexaploidwheat. New Phytologist, 198, 925-937Wang R X, Hai L, Zhang X Y, You G X, Yan C S, Xiao S H. 2009.QTL mapping for grain filling rate and yield-related traits inRILs of the Chinese winter wheat population Heshangmai×Yu8679. Theoretical and Applied Genetics, 118, 313-325Watanabe N. 1983. Variation of D genomes affecting themorphological characters of common wheat. JapaneseJournal of Breeding, 33, 296-302Williams K, Munkvold J, Sorrells M. 2013. Comparison of digitalimage analysis using elliptic Fourier descriptors and majordimensions to phenotype seed shape in hexaploid wheat(Triticum aestivum L.). Euphytica, 190, 99-116Yu J, Pressoir G, Briggs W H, Bi I. V, Yamasaki M, DoebleyJ F, Buckler E S. 2005. A unified mixed-model method forassociation mapping that accounts for multiple levels ofrelatedness. Nature Genetics, 38, 203-208Zhang X, Wang J, Huang J, Lan H, Wang C, Yin C, Wu Y,Tang H, Qian Q, Lin J. 2012. Rare allele of OsPPKL1associated with grain length causes extra-large grain and asignificant yield increase in rice. Proceedings of the NationalAcademy of Sciences of the United States of America, 109,21534-21539 |
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