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| A maize bundle sheath defective mutation mapped on chromosome 1 between SSR markers umc1395 and umc1603 |
| PAN Yu, CHEN Xu-qing, XIE Hua, DENG Lei, LI Xiang-long, ZHANG Xiao-dong, HAN Li-xin, YANG Feng-ping, XUE Jing, ZHANG Li-quan |
1、Beijing Agro-Biotechnology Research Center, Beijing Academy of Agricultural and Forestry Science, Beijing 100097, P.R.China
2、Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education/College of Horticulture and
Landscape Architecture, Southwest University, Chongqing 400715, P.R.China
3、Bioengineering College, Chongqing University, Chongqing 400030, P.R.China |
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摘要 The bsd-pg (bundle sheath defective pale green) mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maize inbred line 501. The characterization was that the biogenesis and development of the chloroplasts was mainly interfered in bundle sheath cells rather than in mesophyll cells. For mapping the bsd-pg, an F2 population was derived from a cross between the mutant bsd-pg and an inbred line Xianzao 17. Using specific locus amplified fragment sequencing (SLAF-Seq) technology, a total of 5 783 polymorphic SLAFs were analysed with 1 771 homozygous alleles between maternal and paternal parents. There were 49 SLAFs, which had a ratio of paternal to maternal alleles of 2:1 in bulked normal lines, and three trait-related candidate regions were obtained on chromosome 1 with a size of 3.945 Mb. For the fine mapping, new simple sequence repeats (SSRs) markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850 934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes. These results provide a foundation for the cloning of bsd-pg by map-based strategy, which is essential for revealing the functional differentiation and coordination of the two cell types, and helps to elucidate a comprehensive understanding of the C4 photosynthesis pathway and related processes in maize leaves.
Abstract The bsd-pg (bundle sheath defective pale green) mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maize inbred line 501. The characterization was that the biogenesis and development of the chloroplasts was mainly interfered in bundle sheath cells rather than in mesophyll cells. For mapping the bsd-pg, an F2 population was derived from a cross between the mutant bsd-pg and an inbred line Xianzao 17. Using specific locus amplified fragment sequencing (SLAF-Seq) technology, a total of 5 783 polymorphic SLAFs were analysed with 1 771 homozygous alleles between maternal and paternal parents. There were 49 SLAFs, which had a ratio of paternal to maternal alleles of 2:1 in bulked normal lines, and three trait-related candidate regions were obtained on chromosome 1 with a size of 3.945 Mb. For the fine mapping, new simple sequence repeats (SSRs) markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850 934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes. These results provide a foundation for the cloning of bsd-pg by map-based strategy, which is essential for revealing the functional differentiation and coordination of the two cell types, and helps to elucidate a comprehensive understanding of the C4 photosynthesis pathway and related processes in maize leaves.
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Received: 23 September 2014
Accepted:
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| Fund: This research was supported by the National Natural Science Foundation of China (30700476 and 31071057) and the Beijing Natural Science Foundation, China (5083021). |
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Corresponding Authors:
CHEN Xu-qing, Tel: +86-10-51503868,Fax: +86-10-51503980, E-mail: chenxuqing@baafs.net.cn
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| About author: PAN Yu, Tel: +86-23-68250974, Mobile: +86-18723494126,Fax: +86-23-68251274, E-mail: yu.pan82@yahoo.com;* These authors contributed equally to this study. |
Cite this article:
PAN Yu, CHEN Xu-qing, XIE Hua, DENG Lei, LI Xiang-long, ZHANG Xiao-dong, HAN Li-xin, YANG Feng-ping, XUE Jing, ZHANG Li-quan.
2015.
A maize bundle sheath defective mutation mapped on chromosome 1 between SSR markers umc1395 and umc1603. Journal of Integrative Agriculture, 14(10): 1949-1957.
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