|
|
|
|
|
| A maize bundle sheath defective mutation mapped on chromosome 1 between SSR markers umc1395 and umc1603 |
| PAN Yu, CHEN Xu-qing, XIE Hua, DENG Lei, LI Xiang-long, ZHANG Xiao-dong, HAN Li-xin, YANG Feng-ping, XUE Jing, ZHANG Li-quan |
1、Beijing Agro-Biotechnology Research Center, Beijing Academy of Agricultural and Forestry Science, Beijing 100097, P.R.China
2、Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education/College of Horticulture and
Landscape Architecture, Southwest University, Chongqing 400715, P.R.China
3、Bioengineering College, Chongqing University, Chongqing 400030, P.R.China |
|
|
|
摘要 The bsd-pg (bundle sheath defective pale green) mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maize inbred line 501. The characterization was that the biogenesis and development of the chloroplasts was mainly interfered in bundle sheath cells rather than in mesophyll cells. For mapping the bsd-pg, an F2 population was derived from a cross between the mutant bsd-pg and an inbred line Xianzao 17. Using specific locus amplified fragment sequencing (SLAF-Seq) technology, a total of 5 783 polymorphic SLAFs were analysed with 1 771 homozygous alleles between maternal and paternal parents. There were 49 SLAFs, which had a ratio of paternal to maternal alleles of 2:1 in bulked normal lines, and three trait-related candidate regions were obtained on chromosome 1 with a size of 3.945 Mb. For the fine mapping, new simple sequence repeats (SSRs) markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850 934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes. These results provide a foundation for the cloning of bsd-pg by map-based strategy, which is essential for revealing the functional differentiation and coordination of the two cell types, and helps to elucidate a comprehensive understanding of the C4 photosynthesis pathway and related processes in maize leaves.
Abstract The bsd-pg (bundle sheath defective pale green) mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maize inbred line 501. The characterization was that the biogenesis and development of the chloroplasts was mainly interfered in bundle sheath cells rather than in mesophyll cells. For mapping the bsd-pg, an F2 population was derived from a cross between the mutant bsd-pg and an inbred line Xianzao 17. Using specific locus amplified fragment sequencing (SLAF-Seq) technology, a total of 5 783 polymorphic SLAFs were analysed with 1 771 homozygous alleles between maternal and paternal parents. There were 49 SLAFs, which had a ratio of paternal to maternal alleles of 2:1 in bulked normal lines, and three trait-related candidate regions were obtained on chromosome 1 with a size of 3.945 Mb. For the fine mapping, new simple sequence repeats (SSRs) markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850 934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes. These results provide a foundation for the cloning of bsd-pg by map-based strategy, which is essential for revealing the functional differentiation and coordination of the two cell types, and helps to elucidate a comprehensive understanding of the C4 photosynthesis pathway and related processes in maize leaves.
|
|
Received: 23 September 2014
Accepted:
|
| Fund: This research was supported by the National Natural Science Foundation of China (30700476 and 31071057) and the Beijing Natural Science Foundation, China (5083021). |
|
Corresponding Authors:
CHEN Xu-qing, Tel: +86-10-51503868,Fax: +86-10-51503980, E-mail: chenxuqing@baafs.net.cn
E-mail: chenxuqing@baafs.net.cn
|
| About author: PAN Yu, Tel: +86-23-68250974, Mobile: +86-18723494126,Fax: +86-23-68251274, E-mail: yu.pan82@yahoo.com;* These authors contributed equally to this study. |
Cite this article:
PAN Yu, CHEN Xu-qing, XIE Hua, DENG Lei, LI Xiang-long, ZHANG Xiao-dong, HAN Li-xin, YANG Feng-ping, XUE Jing, ZHANG Li-quan.
2015.
A maize bundle sheath defective mutation mapped on chromosome 1 between SSR markers umc1395 and umc1603. Journal of Integrative Agriculture, 14(10): 1949-1957.
|
| Bansal K C, Bogorad L. 1993. Cell type-preferred expressionof maize cab-m1: Repression in bundle sheath cells andenhancement in mesophyll cells. Proceedings of theNational Academy of Sciences of the United States ofAmerica, 90, 4057-4061Bastías E, González-Moro M B, González-Murua C. 2013.Interactive effects of excess boron and salinity onhistological and ultrastructural leaves of Zea mays amylaceafrom the Lluta Valley (Arica-Chile). Ciencia e InvestigaciónAgraria, 40, 581-595Beyer K, Lao J I, Alvarez X A, Cacabelos R. 1997. A generalmethod for DNA polymorphism identification in geneticassessment and molecular diagnosis. Methods andFindings in Experimental and Clinical Pharmacology, 19,87-91Brutnell T P, Sawers R J, Mant A, Langdale J A. 1999. BUNDLESHEATH DEFECTIVE2, a novel protein required for posttranslationalregulation of the rbcL gene of maize. The PlantCell, 11, 849-864.Chen S, Huang Z, Dai Y, Qin S, Gao Y, Zhang L, Gao Y, ChenJ. 2013. The development of 7E chromosome-specificmolecular markers for Thinopyrum elongatum based onSLAF-seq technology. PLoS One, 8, e65122.Cribb L, Hall L N, Langdale J A. 2001. Four mutant alleleselucidate the role of the G2 protein in the development ofC-4 and C-3 photosynthesizing maize tissues Genetics,159, 787-797Hall L N, Rossini L, Cribb L, Langdale J A. 1998a. GOLDEN 2:A novel transcriptional regulator of cellular differentiation inthe maize leaf. The Plant Cell, 10, 925-936Hall L N, Roth R, Brutnell T P, Langdale J A. 1998b. Cellulardifferentiation in the maize leaf is disrupted by bundlesheath defective mutations. Symposia of the Society forExperimental Biology, 51, 27-31Holmes R S. 2010. Comparative genomics and proteomics ofvertebrate diacylglycerol acyltransferase (DGAT), acyl CoAwax alcohol acyltransferase (AWAT) and monoacylglycerolacyltransferase (MGAT). Comparative Biochemistry andPhysiology (Part D Genomics Proteomics), 5, 45-54Langdale J A, Kidner C A. 1994. bundle sheath defective, amutation that disrupts cellular differentiation in maize leaves.Development, 120, 673-681Langdale J A, Rothermel B A, Nelson T. 1988. Cellular patternof photosynthetic gene expression in developing maizeleaves. Genes & Development, 2, 106-115Li Y, Shi Y, Cao Y, Wang T. 2005. Establishment of a corecollection for maize germplasm preserved in ChineseNational Genebank using geographic distribution andcharacterization data. Genetic Resources and CropEvolution, 51, 845-852Nelson T, Langdale J A. 1992. Developmental genetics of C4photosynthesis. Annual Review of Plant Physiology andPlant Molecular Biology, 43, 25-47Rossini L, Cribb L, Martin D J, Langdale J A. 2001. The maizegolden2 gene defines a novel class of transcriptionalregulators in plants. The Plant Cell, 13, 1231-1244Roth R, Hall L N, Brutnell T P, Langdale J A. 1996. bundlesheath defective2, a mutation that disrupts the coordinateddevelopment of bundle sheath and mesophyll cells in themaize leaf. The Plant Cell, 8, 915-927Schnable P S, Ware D, Fulton R S, Stein J C, Wei F, PasternakS, Liang C, Zhang J, Fulton L, Graves T A, Minx P, ReilyA D, Courtney L, Kruchowski S S, Tomlinson C, StrongC, Delehaunty K, Fronick C, Courtney B, Rock S M, et al.2009. The B73 maize genome: Complexity, diversity, anddynamics. Science, 326, 1112-1115Sharpe R M, Mahajan A, Takacs E M, Stern D B, Cahoon AB. 2011. Developmental and cell type characterizationof bundle sheath and mesophyll chloroplast transcriptabundance in maize. Current Genetics, 57, 89-102Sharpe R M, Offermann S. 2014. One decade after thediscovery of single-cell C4 species in terrestrial plants:what did we learn about the minimal requirements of C4photosynthesis? Photosynthesis Research, 119, 169-180Sun X, Liu D, Zhang X, Li W, Liu H, Hong W, Jiang C, Guan N,Ma C, Zeng H, Xu C, Song J, Huang L, Wang C, Shi J, WangR, Zheng X, Lu C, Wang X, Zheng H. 2013. SLAF-seq:An efficient method of large-scale de novo SNP discoveryand genotyping using high-throughput sequencing. PLOSONE, 8, e58700.Tausta S L, Li P, Si Y, Gandotra N, Liu P, Sun Q, Brutnell TP, Nelson T. 2014. Developmental dynamics of Kranz celltranscriptional specificity in maize leaf reveals early onsetof C4-related processes. Journal of Experimental Botany,65, 3543-3555Vi?ánková A, Kutík J, Vi?ánková A, Kutík J. 2005. Chloroplastultrastructural development in vascular bundle sheath cellsof two different maize (Zea mays L.) genotypes. Plant Soil& Environment, 51, 491-495Xu F, Sun X, Chen Y, Huang Y, Tong C, Bao J. 2015. Rapididentification of major QTLs associated with rice grain weightand their utilization. PLOS ONE, 10, e0122206. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
