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Journal of Integrative Agriculture  2020, Vol. 19 Issue (4): 931-940    DOI: 10.1016/S2095-3119(19)62644-4
Special Issue: Triticeae Crops Genetics · Breeding · Germplasm Resources
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Molecular detection of the powdery mildew resistance genes in winter wheats DH51302 and Shimai 26
QU Yun-feng1, 2, WU Pei-pei2, HU Jing-huang2, CHEN Yong-xing3, SHI Zhan-liang4, QIU Dan2, LI Ya-hui2, ZHANG Hong-jun2, ZHOU Yang2, YANG Li2, LIU Hong-wei2, ZHU Tong-quan5, LIU Zhi-yong3, ZHANG Yan-ming1, LI Hong-jie
1 Key Laboratory of Molecular Cytogenetic and Genetic Breeding of Heilongjiang Province/College of Life Science and Technology, Harbin Normal University, Harbin 150025, P.R.China
2 National Engineering Laboratory for Crop Molecular Breeding/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
3 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P.R.China
4 Shijiazhuang Academy of Agricultural and Forestry Sciences, Shijiazhuang 050041, P.R.China
5 Wheat Research Institute, Zhumadian Academy of Agricultural Sciences, Zhumadian 463000, P.R.China
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Resistance to powdery mildew is an important trait of interest in many wheat breeding programs.  The information on genes conferring resistance to powdery mildew in wheat cultivars is useful in parental selection.  Winter wheat breeding line DH51302 derived from Liangxing 99 and cultivar Shimai 26 derived from Jimai 22 showed identical infection patterns against 13 isolates of Blumeria graminis f. sp. tritici (Bgt) that causes wheat powdery mildew.  DH51302 and Shimai 26 were crossed to a powdery mildew susceptible cultivar Zhongzuo 9504 and the F2:3 families were used in molecular localization of the resistance genes.  Fourteen polymorphic markers, which were linked to Pm52 from Liangxing 99, were used to establish the genetic linkage maps for the resistance genes PmDH51302 and PmSM26 in DH51302 and Shimai 26, respectively.  These genes were placed in the same genetic interval where Pm52 resides.  Analysis of gene-linked molecular markers indicated that PmDH51302 and PmSM26 differed from other powdery mildew resistance genes on chromosome arm 2BL, such as Pm6, Pm33, Pm51, MlZec1, MlAB10, and Pm64.  Based on the results of reaction patterns to different Bgt isolates and molecular marker localization, together with the pedigree information, DH51302 and Shimai 26 carried the same gene, Pm52, which confers their resistance to powdery mildew.
Keywords:  Triticum aestivum        Blumeria graminis f. sp. tritici        Pm52        molecular mapping  
Received: 03 December 2018   Accepted: 04 March 2020
Fund: The authors thank the financial support provided by the National Key Research and Development Program of China (2017YFD0100600), the National Natural Science Foundation of China (31471491 and 31871621), the Chinese Academy of Agricultural Sciences (CAAS) Innovation Team, and the International Cooperation Project in the Innovative Engineering of CAAS (CAAS-XTCX2018-020-2).
Corresponding Authors:  Correspondence LI Hong-jie, Tel: +86-10-82105321, E-mail:; ZHANG Yan-ming, E-mail:   
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QU Yun-feng, WU Pei-pei, HU Jing-huang, CHEN Yong-xing, SHI Zhan-liang, QIU Dan, LI Ya-hui, ZHANG Hong-jun, ZHOU Yang, YANG Li, LIU Hong-wei, ZHU Tong-quan, LIU Zhi-yong, ZHANG Yan-ming, LI Hong-jie. 2020. Molecular detection of the powdery mildew resistance genes in winter wheats DH51302 and Shimai 26. Journal of Integrative Agriculture, 19(4): 931-940.

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