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| The miR164-TaNAC14 module regulates root development and abiotic-stress tolerance in wheat seedlings |
| CHI Qing*, DU Lin-ying*, MA Wen*, NIU Ruo-yu, WU Bao-wei, GUO Li-jian, MA Meng, LIU Xiang-li, ZHAO Hui-xian# |
College of Life Sciences, Northwest A & F University, Yangling 712100, P.R.China
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摘要
前人研究已经揭示出水稻和拟南芥中 miR164家族和mir164靶向的转录因子基因在发育过程和胁迫应答中扮演着多种角色。我们在小麦中发现的9个tae-miR164遗传位点(tae-mir164a to i)能生成两个miR164成熟序列，它们通过切割各自靶标mRNA的方式使新鉴定的靶基因TaNACs (TaNAC1, TaNAC7, TaNAC11, and TaNAC14)下调表达。小麦tae-miR164或其调控靶基因TaNAC14过表达表明，miR164-TaNAC14模块对小麦幼苗的根系生长发育和非生物胁迫 (干旱和盐碱)耐性有显著影响，TaNAC14促进小麦幼苗根系生长发育，增强耐旱性；而miR164通过下调TaNAC14的表达抑制小麦幼苗根系发育，降低耐旱性和耐盐性。我们研究发现的miR164-TaNAC14模块以及其它tae-miR164调控靶基因，为抗旱小麦育种提供了新的遗传资源。
Abstract
Previous studies have revealed the miR164 family and the miR164-targeted NAC transcription factor genes in rice (Oryza sativa) and Arabidopsis that play versatile roles in developmental processes and stress responses. In wheat (Triticum aestivum L.), we found nine genetic loci of tae-miR164 (tae-MIR164 a to i) producing two mature sequences that down-regulate the expression of three newly identified target genes of TaNACs (TaNAC1, TaNAC11, and TaNAC14) by the cleavage of the respective mRNAs. Overexpression of tae-miR164 or one of its target genes (TaNAC14) demonstrated that the miR164-TaNAC14 module greatly affects root growth and development and stress (drought and salinity) tolerance in wheat seedlings, and TaNAC14 promotes root growth and development in wheat seedlings and enhances drought tolerance, while tae-miR164 inhibits root development and reduces drought and salinity tolerance by down-regulating the expression of TaNAC14. These findings identify the miR164-TaNAC14 module as well as other tae-miR164-regulated genes which can serve as new genetic resources for stress-resistance wheat breeding.
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Received: 10 January 2022
Accepted: 04 March 2022
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| Fund:
This study was financially supported by the National Natural Science Foundation of China (32072003 and 32072059) and the Key Research and Development Program of Shaanxi Province, China (2021NY-079).
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| About author: #Correspondence ZHAO Hui-xian, Tel: +86-29-87092843, Fax: +86-29-87092262, E-mail: hxzhao212@nwafu.edu.cn
* These authors contributed equally to this study. |
Cite this article:
CHI Qing, DU Lin-ying, MA Wen, NIU Ruo-yu, WU Bao-wei, GUO Li-jian, MA Meng, LIU Xiang-li, ZHAO Hui-xian.
2023.
The miR164-TaNAC14 module regulates root development and abiotic-stress tolerance in wheat seedlings. Journal of Integrative Agriculture, 22(4): 981-998.
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