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Journal of Integrative Agriculture  2023, Vol. 22 Issue (1): 108-119    DOI: 10.1016/j.jia.2022.08.099
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Effect of the L-D1 alleles on leaf morphology, canopy structure and photosynthetic productivity in upland cotton (Gossypium hirsutum L.)

JIANG Hui1*, GAO Ming-wei1*, CHEN Ying1, ZHANG Chao1, WANG Jia-bao1, CHAI Qi-chao1, WANG Yong-cui1, ZHENG Jin-xiu2, WANG Xiu-li1, ZHAO Jun-sheng1, 2

1 Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, P.R.China
2 College of Life Sciences, Shandong Normal University, Jinan 250014, P.R.China
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One of the most important objectives for breeders is to develop high-yield cultivars.  The increase in crop yield has met with bottlenecks after the first green revolution, and more recent efforts have been focusing on achieving high photosynthetic efficiency traits in order to enhance the yield.  Leaf shape is a significant agronomic trait of upland cotton that affects plant and canopy architecture, yield, and other production attributes.  The major leaf shape types, including normal, sub-okra, okra, and super-okra, with varying levels of lobe severity, are controlled by a multiple allelic series of the D-genome locus L-D1.  To analyze the effects of L-D1 alleles on leaf morphology, photosynthetic related traits and yield of cotton, two sets of near isogenic lines (NILs) with different alleles were constructed in Lumianyan 22 (LMY22) and Lumianyan 28 (LMY28) backgrounds.  The analysis of morphological parameters and the results of virus-induced gene silencing (VIGS) showed that the regulation of leaf shape by L-D1 alleles was similar to a gene-dosage effect.  Compared with the normal leaf, deeper lobes of the sub-okra leaf improved plant canopy structure by decreasing the leaf area index (LAI) and increasing the light transmittance rate (LTR), and the mid-range LAI of sub-okra leaf also guaranteed the accumulation of cotton biomass.  Although the chlorophyll content (SPAD) of sub-okra leaf was lower than those of the other two leaf shapes, the net photosynthetic rate (Pn) of sub-okra leaf was higher than those of okra leaf and normal leaf at most stages.  Thus, the improvements in canopy structure, as well as photosynthetic and physiological characteristics, contributed to optimizing the light environment, thereby increasing the total biomass and yield in the lines with a sub-okra leaf shape.  Our results suggest that the sub-okra leaf may have practical application in cultivating varieties, and could enhance sustainable and profitable cotton production.

Keywords:  photosynthesis        canopy structure        yield        biomass        sub-okra leaf shape  
Received: 23 July 2021   Accepted: 09 October 2021
Fund: This work was supported by the State Key Laboratory of Cotton Biology Open Fund, China (CB2021A18), the Youth Scientific Research Foundation of Shandong Academy of Agricultural Sciences, China (2016YQN09), the Improved Variety Project of Shandong Province, China (2020LZGC002) and the China Agriculture Research System of MOF and MARA (CARS-15-05).
About author:  Correspondence WANG Xiu-li, E-mail:; ZHAO Jun-sheng, Tel: +86-531-66658256, E-mail: * These authors contributed equally to this study.

Cite this article: 

JIANG Hui, GAO Ming-wei, CHEN Ying, ZHANG Chao, WANG Jia-bao, CHAI Qi-chao, WANG Yong-cui, ZHENG Jin-xiu, WANG Xiu-li, ZHAO Jun-sheng. 2023. Effect of the L-D1 alleles on leaf morphology, canopy structure and photosynthetic productivity in upland cotton (Gossypium hirsutum L.). Journal of Integrative Agriculture, 22(1): 108-119.

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