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Journal of Integrative Agriculture  2022, Vol. 21 Issue (10): 2973-2983    DOI: 10.1016/j.jia.2022.07.048
Special Issue: 线虫合辑Nematology
Plant Protection Advanced Online Publication | Current Issue | Archive | Adv Search |
A fragment of a 70-kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant protein GmSHMT08
LIU Zhi1, 2, ZHANG Liu-ping1, ZHAO Jie1, JIAN Jin-zhuo1, PENG Huan1, HUANG Wen-kun1KONG Ling-an1PENG De-liang1, LIU Shi-ming1

1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

2 MARA–CABI joint laboratory for bio-safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

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摘要  

大豆孢囊线虫(SCN, Heterodera glycines)严重制约大豆生产。大豆抗线虫数量性状遗传位点Rhg4上的丝氨酸羟甲基转移酶编码基因(GmSHMT08)对大豆孢囊线虫有显著的抗性,但该基因如何介导了对大豆孢囊线虫的抗性机制仍不明晰,GmSHMT08能否与大豆孢囊线虫产生的蛋白发生互作仍不明确。本研究以GmSHMT08作为诱饵,通过酵母双杂交体系在线虫中筛选出了与GmSHMT08互作的一个热休克蛋白70片段(HgHSP70p)。通过GST pull-down和荧光双分子互补,进一步验证了HgHSP70p与GmSHMT08之间存在互作关系。本研究发现的HgHSP70基因可以作为关键候选基因,用于进一步探究GmSHMT08介导的对大豆孢囊线虫的抗性机制。



Abstract  


Soybean cyst nematode (SCN) Heterodera glycines is considered as the major constraint to soybean production.  GmSHMT08 at Rhg4 locus on chromosome 08, encoding a serine hydroxylmethyltransferase, is a major gene underlying resistance against Hglycines in Peking-type soybeans.  However, the molecular mechanism underpinning this resistance is less well characterized, and whether GmSHMT08 could interact with proteins in Hglycines remains unclear.  In this study, yeast two-hybrid screening was conducted using GmSHMT08 as a bait protein, and a fragment of a 70-kDa heat shock protein (HgHSP70) was screened from Hglycines that exhibited interaction with GmSHMT08.  This interaction was verified by both GST pull-down and bimolecular fluorescence complementation assays.  Our finding reveals HgHSP70 could be applied as a potential candidate gene for further exploring the mechanism on GmSHMT08-mediated resistance against SCN Hglycines.


Keywords:  soybean cyst nematode       Heterodera glycines       GmSHMT08       HgHSP70       interaction       resistance  
Received: 31 August 2021   Accepted: 09 March 2022
Fund: 

This study is supported by the National Natural Science Foundation of China (31972248) and the Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences, China (ASTIP-02-IPP-04). 

About author:  LIU Zhi, E-mail: liuzhi7667@hotmail.com; Correspondence LIU Shi-ming, E-mail: liushiming01@caas.cn; PENG De-liang, E-mail: pengdeliang@caas.cn

Cite this article: 

LIU Zhi, ZHANG Liu-ping, ZHAO Jie, JIAN Jin-zhuo, PENG Huan, HUANG Wen-kun, KONG Ling-an, PENG De-liang, LIU Shi-ming. 2022. A fragment of a 70-kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant protein GmSHMT08. Journal of Integrative Agriculture, 21(10): 2973-2983.

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