|A fragment of a 70-kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant protein GmSHMT08
|LIU Zhi1, 2, ZHANG Liu-ping1, ZHAO Jie1, JIAN Jin-zhuo1, PENG Huan1, HUANG Wen-kun1, KONG Ling-an1, PENG De-liang1, LIU Shi-ming1
1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2 MARA–CABI joint laboratory for bio-safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
大豆孢囊线虫（SCN, Heterodera glycines）严重制约大豆生产。大豆抗线虫数量性状遗传位点Rhg4上的丝氨酸羟甲基转移酶编码基因（GmSHMT08）对大豆孢囊线虫有显著的抗性，但该基因如何介导了对大豆孢囊线虫的抗性机制仍不明晰，GmSHMT08能否与大豆孢囊线虫产生的蛋白发生互作仍不明确。本研究以GmSHMT08作为诱饵，通过酵母双杂交体系在线虫中筛选出了与GmSHMT08互作的一个热休克蛋白70片段（HgHSP70p）。通过GST pull-down和荧光双分子互补，进一步验证了HgHSP70p与GmSHMT08之间存在互作关系。本研究发现的HgHSP70基因可以作为关键候选基因，用于进一步探究GmSHMT08介导的对大豆孢囊线虫的抗性机制。
Soybean cyst nematode (SCN) Heterodera glycines is considered as the major constraint to soybean production. GmSHMT08 at Rhg4 locus on chromosome 08, encoding a serine hydroxylmethyltransferase, is a major gene underlying resistance against H. glycines in Peking-type soybeans. However, the molecular mechanism underpinning this resistance is less well characterized, and whether GmSHMT08 could interact with proteins in H. glycines remains unclear. In this study, yeast two-hybrid screening was conducted using GmSHMT08 as a bait protein, and a fragment of a 70-kDa heat shock protein (HgHSP70) was screened from H. glycines that exhibited interaction with GmSHMT08. This interaction was verified by both GST pull-down and bimolecular fluorescence complementation assays. Our finding reveals HgHSP70 could be applied as a potential candidate gene for further exploring the mechanism on GmSHMT08-mediated resistance against SCN H. glycines.
Received: 31 August 2021
Accepted: 09 March 2022
This study is supported by the National Natural Science Foundation of China (31972248) and the Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences, China (ASTIP-02-IPP-04).
|About author: LIU Zhi, E-mail: firstname.lastname@example.org; Correspondence LIU Shi-ming, E-mail: email@example.com; PENG De-liang, E-mail: firstname.lastname@example.org
Cite this article:
LIU Zhi, ZHANG Liu-ping, ZHAO Jie, JIAN Jin-zhuo, PENG Huan, HUANG Wen-kun, KONG Ling-an, PENG De-liang, LIU Shi-ming.
A fragment of a 70-kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant protein GmSHMT08. Journal of Integrative Agriculture, 21(10): 2973-2983.
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