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Special Issue:
线虫合辑Nematology
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| A fragment of a 70-kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant protein GmSHMT08 |
| LIU Zhi1, 2, ZHANG Liu-ping1, ZHAO Jie1, JIAN Jin-zhuo1, PENG Huan1, HUANG Wen-kun1, KONG Ling-an1, PENG De-liang1, LIU Shi-ming1 |
1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2 MARA–CABI joint laboratory for bio-safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
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摘要
大豆孢囊线虫（SCN, Heterodera glycines）严重制约大豆生产。大豆抗线虫数量性状遗传位点Rhg4上的丝氨酸羟甲基转移酶编码基因（GmSHMT08）对大豆孢囊线虫有显著的抗性，但该基因如何介导了对大豆孢囊线虫的抗性机制仍不明晰，GmSHMT08能否与大豆孢囊线虫产生的蛋白发生互作仍不明确。本研究以GmSHMT08作为诱饵，通过酵母双杂交体系在线虫中筛选出了与GmSHMT08互作的一个热休克蛋白70片段（HgHSP70p）。通过GST pull-down和荧光双分子互补，进一步验证了HgHSP70p与GmSHMT08之间存在互作关系。本研究发现的HgHSP70基因可以作为关键候选基因，用于进一步探究GmSHMT08介导的对大豆孢囊线虫的抗性机制。
Abstract
Soybean cyst nematode (SCN) Heterodera glycines is considered as the major constraint to soybean production. GmSHMT08 at Rhg4 locus on chromosome 08, encoding a serine hydroxylmethyltransferase, is a major gene underlying resistance against H. glycines in Peking-type soybeans. However, the molecular mechanism underpinning this resistance is less well characterized, and whether GmSHMT08 could interact with proteins in H. glycines remains unclear. In this study, yeast two-hybrid screening was conducted using GmSHMT08 as a bait protein, and a fragment of a 70-kDa heat shock protein (HgHSP70) was screened from H. glycines that exhibited interaction with GmSHMT08. This interaction was verified by both GST pull-down and bimolecular fluorescence complementation assays. Our finding reveals HgHSP70 could be applied as a potential candidate gene for further exploring the mechanism on GmSHMT08-mediated resistance against SCN H. glycines.
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Received: 31 August 2021
Accepted: 09 March 2022
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| Fund:
This study is supported by the National Natural Science Foundation of China (31972248) and the Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences, China (ASTIP-02-IPP-04).
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| About author: LIU Zhi, E-mail: liuzhi7667@hotmail.com; Correspondence LIU Shi-ming, E-mail: liushiming01@caas.cn; PENG De-liang, E-mail: pengdeliang@caas.cn |
Cite this article:
LIU Zhi, ZHANG Liu-ping, ZHAO Jie, JIAN Jin-zhuo, PENG Huan, HUANG Wen-kun, KONG Ling-an, PENG De-liang, LIU Shi-ming.
2022.
A fragment of a 70-kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant protein GmSHMT08. Journal of Integrative Agriculture, 21(10): 2973-2983.
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