|
|
|
|
|
| Detection and characterization of an isolate of Tomato mottle mosaic virus infecting tomato in China |
| ZHAN Bin-hui1, CAO Ning1, WANG Kai-na1, ZHOU Xue-ping1, 2 |
1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, China Academy of Agricultural Sciences, Beijing 100193, P.R.China
2 State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China |
|
|
|
摘要 Received 6 December, 2017 Accepted 23 January, 2018
© 2018 CAAS. Publishing services by Elsevier B.V. All rights reserved.
doi:
Abstract Tomato (Solanum lycopersicum) plants exhibiting severe leaf distortion, mottle and systemic crinkling symptoms were identified in Hainan province in China in 2016. To survey and control the disease, it is necessary to identify and characterize the pathogen causing the disease. Dot enzyme-linked immunosorbent assay showed that the crude saps of the infected tomato samples reacted positively with the monoclonal antibody against Tobacco mosaic virus which indicated that one or more tobamoviruses are likely associated with the disease. RT-PCR and DNA sequence analysis results further elucidated that Tomato mottle mosaic virus (ToMMV) in Tobamovirus was the pathogen causing the mottle disease in tomato. We amplified and sequenced the full-length sequence of the genome which showed the highest nucleotide identity with ToMMV YYMLJ and ToMMV TiLhaLJ isolates. The putative virus isolate was named ToMMV Hainan. Biological indexing studies showed that ToMMV Hainan can infect Nicotiana benthamiana, Capsicum annuum and Solanum lycopersicum showing serious symptoms. This was the first identification and characterization of ToMMV infecting tomato in Hainan of China.
|
|
Received: 06 December 2017
Accepted:
|
| Fund: This research was supported by the Agricultural Science and Technology Innovation Program, China (ASTIP). |
|
Corresponding Authors:
Correspondence ZHOU Xue-ping, E-mail: xpzhou@ippcaas.cn, zzhou@zju.edu.cn
|
| About author: ZHAN Bin-hui, E-mail: binhuizhan@126.com; |
Cite this article:
ZHAN Bin-hui, CAO Ning, WANG Kai-na, ZHOU Xue-ping.
2018.
Detection and characterization of an isolate of Tomato mottle mosaic virus infecting tomato in China. Journal of Integrative Agriculture, 17(05): 1207-1212.
|
| Ambrós S, Martínez F, Ivars P, Hernández C, Iglesia F D L, Elena S F. 2017. Molecular and biological characterization of an isolate of Tomato mottle mosaic virus (ToMMV) infecting tomato and other experimental hosts in eastern Spain. European Journal of Plant Pathology, 149, 261–268.Brunt A A, Crabtree K, Dallwitz M J, Gibbs A J, Watson L. 1996. Plant Viruses Online: Descriptions and Lists from the Vide Database. 16th ed. Australian National University, Canberra, Australia.Chng C G, Wong S M, Mahtani P H, Loh C S, Goh C J, Kao M C, Chung M C, Watanabe Y. 1996. The complete sequence of a Singapore isolate of odontoglossum ringspot virus and comparison with other tobamoviruses. Gene, 171, 155–161.Fillmer K, Adkins S, Pongam P, D’Elia T. 2015. Complete genome sequence of a tomato mottle mosaic virus isolate from the United States. Genome Announcements, 3, e00167–e00182.Gibbs A J, Armstrong J S, Gibbs M J. 2004. A type of nucleotide motif that distinguishes tobamovirus species more efficiently than nucleotide signatures. Archives of Virology, 149, 1941–1954.Hanssen I M, Lapidot M, Thomma B P. 2010. Emerging viral diseases of tomato crops. Molecular Plant-Microbe Interactions, 23, 539–548.Li N, Chen Z, Liu Y, Liu Y, Zhou X, Wu J. 2015. Development of monoclonal antibodies and serological assays specific for barley yellow dwarf virus GAV strain. Virology Journal, 12, 136.Li R, Gao S, Fei Z, Ling K S. 2013. Complete genome sequence of a new tobamovirus naturally infecting tomatoes in Mexico. Genome Announcements, 1, e00794–e00807.Li Y, Wang Y, Hu J, Xiao L, Tan G, Lan P, Liu Y, Li F. 2017. The complete genome sequence, occurrence and host range of Tomato mottle mosaic virus Chinese isolate. Virology Journal, 14, 15.Li Y Y, Wang C L, Xiang D, Li R H, Liu Y, Li F. 2014. First report of tomato mottle mosaic virus infection of pepper in China. Plant Disease, 98, 1447.Luria N, Smith E, Reingold V, Bekelman I, Lapidot M, Levin I, Elad N, Tam Y, Sela N, Abu-Ras A. 2017. A new Israeli tobamovirus isolate infects tomato plants harboring Tm-22 resistance genes. PLoS ONE, 12, e0170429.Moreira S R, Eiras M, Chaves A L R, Galleti S R, Colariccio A. 2003. Characterization of a new Tomato mosaic virus strain isolated from tomato in the State of São Paulo, Brazil. Fitopatologia Brasileira, 28, 602–607. (in Portuguese)Padmanabhan C, Yi Z, Li R, Martin G B, Fei Z, Ling K S. 2015. Complete genome sequence of a tomato-infecting tomato mottle mosaic virus in New York. Genome Announcements, 3, e01523–e01538.Pirovano W, Miozzi L, Boetzer M, Pantaleo V. 2014. Bioinformatics approaches for viral metagenomics in plants using short RNAs: model case of study and application to a Cicer arietinum population. Frontiers in Microbiology, 5, 790.Richards K, Jonard G, Guilley H, Keith G. 1977. Leader sequence of 71 nucleotides devoid of G in tobacco mosaic virus RNA. Nature, 267, 548–550.Salem N, Mansour A, Ciuffo M, Falk B W, Turina M. 2016. A new tobamovirus infecting tomato crops in Jordan. Archives of Virology, 161, 503–506.Sui X, Zheng Y, Li R, Padmanabhan C, Tian T, Groth-Helms D, Keinath A P, Fei Z, Wu Z, Ling K S. 2017. Molecular and biological characterization of Tomato mottle mosaic virus and development of RT-PCR detection. Plant Disease, 101, 704–711.Turina M, Geraats B P J, Ciuffo M. 2016. First report of Tomato mottle mosaic virus in tomato crops in Israel. New Disease Reports, 33, 1.Webster C G, Rosskopf E N, Lucas L, Mellinger H C, Adkins S. 2014. First report of tomato mottle mosaic virus infecting tomato in the United States. Plant Health Progress, 15, 151–152.Xu C, Sun X, Taylor A, Jiao C, Xu Y, Cai X, Wang X, Ge C, Pan G, Wang Q, Fei Z, Wang Q. 2017. Diversity, distribution, and evolution of tomato viruses in China uncovered by small RNA sequencing. Journal of Virology, 91, e00173–e00190.Zhang Z C, Lei C Y, Zhang L F, Yang X X, Chen R, Zhang D S. 2008. The complete nucleotide sequence of a novel Tobamovirus, Rehmannia mosaic virus. Archives of virology, 153, 595–599. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
