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Special Issue:
植物病理合辑Plant Protection—Plant Pathology
植物病毒合辑Plant Virus
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Monoclonal antibody-based serological detection of potato virus M in potato plants and tubers |
ZHANG Yu1, GAO Yan-ling2, HE Wan-qin1, WANG Ya-qin1, QIAN Ya-juan1, ZHOU Xue-ping1, 3, WU Jian-xiang1 |
1 State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China
2 Potato Research Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.China
3 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China |
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Abstract Potato virus M (PVM) is one of the common and economically important potato viruses in potato-growing regions worldwide. To investigate and control this viral disease, efficient and specific detection techniques are needed. In this study, PVM virions were purified from infected potato plants and used as the immunogen to produce hybridomas secreting PVM-specific monoclonal antibodies (MAbs). Four highly specific and sensitive murine MAbs, i.e., 1E1, 2A5, 8A1 and 17G8 were prepared through a conventional hybridoma technology. Using these four MAbs, we have developed an antigen-coated plate (ACP)-ELISA, a dot-ELISA and a Tissue print-ELISA for detecting PVM infection in potato plants and tubers. PVM could be detected in infected potato plant tissue crude extracts diluted at 1:10 240 (w/v, g mL–1) by the dot-ELISA or at 1:163 840 (w/v, g mL–1) by the ACP-ELISA. The Tissue print-ELISA is the quickest and easiest assay among the three established serological assays and is more suitable for onsite large-scale sample detection. Detection results of the field-collected samples showed that PVM is currently widespread in the Yunnan and the Heilongjiang provinces in China. The field sample test results of the developed serological assays were supported by the results from RT-PCR and DNA sequencing. We consider that the newly established ACP-ELISA, dot-ELISA and Tissue print-ELISA can benefit PVM detection in potato plant and tuber samples and field epidemiological studies of PVM. These assays can also facilitate the production of virus-free seed potatoes and breeding for PVM-resistant potato cultivars, leading to the successful prevention of this potato viral disease.
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Received: 17 February 2019
Accepted:
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| Fund: We are grateful to Dr. Ding Xinshun (Samuel Roberts Noble Foundation [Retired], Ardmore, USA) for his valuable comments and manuscript edits. This work was supported by the National Key Research and Development Project of China (2017YFD0201604) and the National Natural Science Foundation of China (31571976). |
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Corresponding Authors:
Correspondence WU Jian-xiang, Tel: +86-571-88982250, E-mail: wujx@zju.edu.cn; ZHOU Xue-ping, E-mail: zzhou@zju.edu.
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| About author: ZHANG Yu, E-mail: 380175146@qq.com; cn
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Cite this article:
ZHANG Yu, GAO Yan-ling, HE Wan-qin, WANG Ya-qin, QIAN Ya-juan, ZHOU Xue-ping, WU Jian-xiang .
2020.
Monoclonal antibody-based serological detection of potato virus M in potato plants and tubers . Journal of Integrative Agriculture, 19(5): 1283-1291.
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Agindotan B O, Shiel P J, Berger P H. 2007. Simultaneous detection of potato viruses, PLRV, PVA, PVX and PVY from dormant potato tubers by TaqMan real-time RT-PCR. Journal of Virological Methods, 142, 1–9.
Cavileer T D, Clarke R C, Corsini D L, Berger P H. 1998. A new strain of potato carlavirus M. Plant Disease, 82, 98–102.
Cerovska N, Moravec T, Plchova H, Hoffmeisterova H, Dedic P. 2012. Production of polyclonal antibodies to the recombinant Potato virus M (PVM) non-structural triple gene block protein 1 and coat protein. Journal of Phytopathology, 160, 251–254.
Crosslin J M, Hamlin L L. 2011. Standardized RT-PCR conditions for detection and identification of eleven viruses of potato and potato spindle tuber viroid. American Journal of Potato Research, 88, 333–338.
Elliot J L, Andrew J D, Sead S, Stuart G S, Peter S. 2017. The ICTV report. Virus taxonomy: The classification and nomenclature of viruses. [2018-2-18]. https://talk.ictvonline.org/taxonomy/
Flatken S, Ungewickell V, Menzel W, Maiss E. 2008. Construction of an infectious full-length cDNA clone of potato virus M. Archives of Virology, 153, 1385–1389.
Ge B B, He Z, Jiang D M, Zhang Z X, Liu G J, Wang H Q. 2012. Characterization and complete nucleotide sequence of potato virus M isolated from tomato in China. Acta Virologica, 56, 261–263.
Gramstat A, Courtpzanis A, Rhode W. 1990. The 12 kDa protein of potato virus M displays properties of a nucleic acid binding protein. FEBS Letters, 276, 34–38.
He Z, Larkin R P, Honeycutt W. 2012. Sustainable potato production: Global case studies II. In: Developing Integrated Pest Management for Potato: Experiences and Lessons from Two Distinct Potato Production Systems of Peru. Springer, Netherlands. pp. 419–450.
Jeffries C J. 1998. FAO/IPGRI Technical Guidelines for the Safe Movement of Germplasm: No. 19, Potato. Food and Agriculture Organization of the United Nations. Rome/International Plant Genetic Resources Institute, Rome.
Ju H J, Samuels T D, Wang Y S, Blancaflor E, Payton M, Mitra R, Krishnamurthy K, Nelson R S. 2005. The potato virus X TGBp2 movement protein associates with endoplasmic reticulum-derived vesicles during virus infection. Plant Physiology, 138, 1877–1895.
Koonin E V, Dolja V V. 1993. Evolution and taxonomy of positive-strand RNA viruses: Implications of comparative analysis of amino acid sequences. Critical Reviews in Biochemistry and Molecular Biology, 28, 375–430.
Liu H, Song X J, Ni Y Q, Lu L N, Zhou X P, Wu J X. 2014. Highly sensitive and specific monoclonal antibody-based serological methods for Rice ragged stunt virus detection in rice plants and rice brown planthopper vectors. Journal of Integrative Agriculture, 13, 1943–1951.
Liu Z, Sunzhu Y J, Zhou X P, Jian H, Wu J X. 2017. Monoclonal antibody-based serological detection of Citrus yellow vein clearing virus in citrus groves. Journal of Integrative Agriculture, 16, 884–891.
Loebenstein G, Gaba V. 2012. Viruses of potato. Advances in Virus Research, 84, 209–246.
Mao Y Z. 2009. Survey of potato virus detection technique. China Vegetables, 12, 1–6. (in Chinese)
Morozov S Y, Miroshnichenko N A, Solovyev A G, Zelenina D A, Fedorkin O N, Lukasheva L I, Grachev S A, Chernov B K. 1991. Evidence for two nonoverlapping functional domains in the potato virus X 25 K movement protein. Virology, 183, 782–785.
Rozanov M N, Koonin E V, Gorbalenya A E. 1992. Conservation of the putative methyltransferase domain: A hallmark of the ‘Sindbis-like’ supergroup of positive-strand RNA viruses. Journal of General Virology, 73, 2129–2134.
Salazar L F. 1996. Potato Viruses and their Control. International Potato Center (CIP), Lima, Peru. p. 214.
Schultz E, Folsom D. 1923. Transmission, variation, and control of certain degeneration diseases of Irish potatoes. Journal of Agricultural Research, 25, 43–118.
Senshu H, Yamaji Y, Minato N, Shiraishi T, Maejima K, Hashimoto M, Miura C, Neriya Y, Namba S. 2011. A dual strategy for the suppression of host antiviral silencing: Two distinct suppressors for viral replication and viral movement encoded by potato virus M. Journal of Virology, 85, 10269–10278.
Singh R P, Boucher A, Somerville T H, Coleman S. 1996. Detection of potato viruses A, M, S, X, Y and leafroll and potato spindle tuber viroid from tissue culture plantlets using single leaf discs. American Potato Journal, 73, 101–112.
Shang H L, Xie Y, Zhou X P, Qian Y J, Wu J X. 2011. Monoclonal antibody-based serological methods for detection of Cucumber green mottle mosaic virus. Virology Journal, 8, 228.
Song G, Wu J Y, Xie Y, Liu Y, Qian Y J, Zhou X P, Wu J X. 2017. Monoclonal antibody-based serological assays for detection of Potato virus S in potato plants. Journal of Zhejiang University (Science B: Biomedicine & Biotechnology), 18, 1075–1082.
Tabasinejad F, Jafarpour B, Zakiaghl M, Siampour M, Rouhani H, Mehrvar M. 2014. Genetic structure and molecular variability of potato virus M populations. Archives of Virology, 159, 2081–2090.
Wetter C. 1972. Potato Virus M, Descriptions of Plant Viruses. Vol. 87. Commonwealth Mycology Institute/Association of Applied Biology, Kew, England. p. 87.
Wu J X, Meng C M, Shang H L, Rong S, Zhang C, Hong J, Zhou X P. 2011. Monoclonal antibody-based triple antibody sandwich-enzyme-linked immunosorbent assay and immunocapture reverse transcription-polymerase chain reaction for Odontoglossum ringspot virus detection. Journal of Virological Methods, 171, 40–45.
Wu J X, Ni Y, Liu H, Ding M, Zhou X P. 2014. Monoclonal antibody-based serological assays and immunocapture-RT-PCR for detecting Rice dwarf virus in field rice plants and leafhopper vectors. Journal of Virological Methods, 195, 134–140.
Wu J X, Wang Q, Liu H, Qian Y J, Xie Y, Zhou X P. 2013. Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China. Journal of Zhejiang University (Science B: Biomedicine & Biotechnology), 14, 555–562.
Xu H, D’Aubin J, Nie J. 2010. Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes. Virology Journal, 7, 25.
Zavriev S K, Kanyuka K V, Levay K E. 1991. The genome organization of potato virus M RNA. Journal of General Virology, 72, 9–14
Zhang M H, Chen R, Zhou X P, Wu J X. 2018. Monoclonal antibody-based serological detection methods for Wheat dwarf virus. Virologica Sinica, 33,162–172.
Zhou X P, Chen J S, Li D B. 1994. A method for high yield purification of potyviruses. Microbiology, 21, 184–186. (in Chinese) |
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