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Journal of Integrative Agriculture  2019, Vol. 18 Issue (5): 1170-1176    DOI: 10.1016/S2095-3119(19)62614-6
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Impacts of Sletr1-1 and Sletr1-2 mutations on the hybrid seed quality of tomatoes
Syariful Mubarok1, Hiroshi Ezura2, Anas1, Kusumiyati1, Neni Rostini1, Erni Suminar1, Gungun Wiguna3, 4  
1 Department of Agronomy, Universitas Padjadjaran, Sumedang 45363, Indonesia
2 Tsukuba Plant Innovation Research Center, University of Tsukuba, Tsukuba 305-8572, Japan 
3 Graduate Program of Plant Science, Faculty of Agriculture, Universitas Padjadjaran, Sumedang 45363, Indonesia
4 Vegetable Crops Research Institute, Ministry of Agriculture, Bandung 40391, Indonesia
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Shelf life is an important breeding trait in tomato, especially for the tomato production in subtropical and tropical regions.  Previously we have isolated and characterized ethylene receptor mutants, Sletr1-1 and Sletr1-2 from mutant population based on Micro-Tom cultivar.  Sletr1-1 showed insensitivity to ethylene while Sletr1-2 showed reduced sensitivity to ethylene.  We also have demonstrated that the traits are useful for extending fruit shelf life of the hybrid tomato cultivars.  For commercializing the hybrid cultivars, the seed quality is another important trait.  In this study, we evaluated the effects of the Sletr1-1 and Sletr1-2 mutations on the seed quality characteristics of F1 hybrid lines generated by crossing Sletr1-1 and Sletr1-2 with three commercial tomato cultivars, Intan, Mutiara and Ratna.  Sletr1-1 mutation conferred insensitivity to ethylene in the F1 hybrid seedlings, resulting in negative effects including reduced germination rate, vigor index and emergence speed index.  Interestingly Sletr1-2 mutation had almost no effect on the seed quality characteristics of the F1 hybrid lines, suggesting that Sletr1-2 was suitable for producing high quality of hybrid seeds.
Keywords:  ethylene receptor        mutant        hybrid quality seed        tomato  
Received: 11 May 2018   Accepted: 29 April 2019
Fund: This work was supported by a grant of Superior Applied Research of Higher Education (006/ADD/SP2H/DRPM/VIII/2017) from the Ministry of Research, Technology and Higher Education, Indonesia.
Corresponding Authors:  Correspondence Syariful Mubarok, Tel: +62-22-7796320, Fax: +62-22-7796316, E-mail:   
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Syariful Mubarok, Hiroshi Ezura, Anas, Kusumiyati, Neni Rostini, Erni Suminar, Gungun Wiguna. 2019. Impacts of Sletr1-1 and Sletr1-2 mutations on the hybrid seed quality of tomatoes. Journal of Integrative Agriculture, 18(5): 1170-1176.

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