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Journal of Integrative Agriculture  2017, Vol. 16 Issue (11): 2482-2498    DOI: 10.1016/S2095-3119(17)61688-5
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Morphological and ISSR molecular markers reveal genetic diversity of wild hawthorns (Crataegus songorica K. Koch.) in Xinjiang, China
SHENG Fang1, CHEN Shu-ying2, TIAN Jia1, LI Peng1, QIN Xue1, WANG Lei3, LUO Shu-ping1, LI Jiang1
1 College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, P.R.China
2 Academy of Forestry in Ili, Yining 835000, P.R.China
3 Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, P.R.China
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Abstract  The wild hawthorn species, Crataegus songorica K. Koch., is an important wild germplasm resource in Xinjiang, China that has been endangered in recent years.  The genetic diversity of C. songorica K. Koch. germplasm in five populations from Daxigou, Xinjiang, China were evaluated based on phenotypic traits and ISSR molecular markers to provide basic information on resource protection, rational utilization and genetic improvement.  The F-value for the phenotypic differentiation coefficient of the 33 traits measured ranged from 0.266 to 15.128, and mean value was 13.85%.  The variation among populations was found to be lower than that within population.  A total of 303 loci were detected within the five populations by 12 primers.  Within 298 polymorphic loci, the polymorphism was 98.35%, showing a high genetic diversity in C. songorica K. Koch.  The gene diversity within population, total population genetic diversity, genetic differentiation coefficient and gene flow were 0.2779, 0.3235, 0.1408, and 3.0511, respectively.  Our results showed that C. songorica K. Koch. from Xinjiang has a high level of genetic diversity at both the phenotypic and molecular levels.  Significant genetic differentiation existed within population and the differentiation trend showed a regional association.  And in this study, in situ and ex situ conservation approaches were raised for wild hawthorn protection utilization.
Keywords:  phenotypic traits        ISSR marker        genetic diversity        Crataegus songorica K. Koch.        germplasm resources        molecular marker  
Received: 04 January 2017   Accepted:

This work was supported by the Special Research Projects of National Forestry Industry of Public Bene?t, China (201304701-1), the Key Discipline of Horticultural of Xinjiang Uygur Autonomous Region of China (2016-10758-3), and the Key Laboratory of Characteristics of Fruit Trees Center of Xinjiang Agricultural University, China.

Corresponding Authors:  Correspondence LI Jiang, Tel/Fax: +86-0991-8762363, E-mail:; TIAN Jia, E-mail:    
About author:  SHENG Fang, E-mail:

Cite this article: 

SHENG Fang, CHEN Shu-ying, TIAN Jia, LI Peng, QIN Xue, WANG Lei, LUO Shu-ping, LI Jiang. 2017. Morphological and ISSR molecular markers reveal genetic diversity of wild hawthorns (Crataegus songorica K. Koch.) in Xinjiang, China. Journal of Integrative Agriculture, 16(11): 2482-2498.

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