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Journal of Integrative Agriculture  2021, Vol. 20 Issue (5): 1302-1313    DOI: 10.1016/S2095-3119(20)63371-8
Special Issue: 植物抗病遗传合辑Plant Disease-resistance Genetics
Plant Protection Advanced Online Publication | Current Issue | Archive | Adv Search |
Identification of leaf rust resistance genes in common wheat varieties from China and foreign countries
LIU Yuan1, Takele Weldu GEBREWAHID1, 2, ZHANG Pei-pei1, LI Zai-feng1, LIU Da-qun1 
1 College of Plant Protection, Hebei Agricultural University, Baoding 071001, P.R.China
2 College of Agriculture, Aksum University, Shire-Indaslassie 314, Ethiopia
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小麦叶锈病是危害小麦最严重的病害之一,该病由小麦叶锈菌(Puccinia recondite f. sp. tritici)引起,侵染小麦叶片,严重影响小麦光合作用从而造成减产,培育和利用小麦抗叶锈病品种是最为经济有效的防治措施。为鉴定国内外普通小麦材料中所携带的抗病基因并了解其组成背景,发掘新的抗叶锈病基因,本研究结合基因推导、分子标记检测等方法对66个来自世界不同国家的普通小麦品种(系)进行了抗叶锈病基因鉴定。在苗期接种17个不同毒力的小麦叶锈菌生理小种,通过与36个含有单个已知抗病基因的载体品种抗性比较进行基因推导。同时,利用12个与已知抗病基因紧密连锁的分子标记对供试品种(系)进行基因标记检测,以验证基因推导结果。在河南周口(2016-2017年度)、河北保定(2017-2018年度)两地对66个供试品种(系)进行小麦叶锈病田间叶锈抗性调查,利用SAS软件对鉴定数据进行分析,以筛选出具有成株慢锈性的品种。结果表明,在66个供试品种(系)中,12个品种(系)含有Lr1,4个品种(系)含有Lr26,3个品种含有Lr10,2个品种含有Lr20,2个品种含有Lr17。通过成株抗病基因分子标记检测发现,14个品种(系)含有Lr34,5个品种含有Lr46,3个品种(系)含有Lr37。结合田间调查结果发现,有17个品种(系)具有成株慢锈性。这些含有已知抗病基因的品种及慢锈性品种将丰富我国现有的小麦种质资源,有利于培育抗病品种,同时为利用基因布局防治小麦叶锈病提供遗传学依据。

Wheat leaf rust, triggered by Puccinia triticina Eriks (Pt), is among the most important diseases of wheat worldwide.  Deploying resistant varieties against leaf rust is the most effective, environmentally-friendly and economic way to control the disease.  In the present study, 66 wheat varieties form China and foreign countries were tested with 17 Pt races for gene postulation during the seedling stage in the greenhouse.  All the varieties were also planted to identify slow rusting responses to leaf rust at the adult plant stage in Baoding and Zhoukou field trials during the 2016/2017 to 2017/2018 cropping seasons.  Moreover, 12 closely linked molecular markers to known leaf rust resistance (Lr) genes were used for assessing all the varieties.  The results of both gene postulation and molecular marker identification showed that a total of eight Lr genes, Lr1, Lr10, Lr17, Lr20, Lr26, Lr34, Lr37 and Lr46, either singly or in combination were detected in 32 varieties.  Known Lr genes were not identified in the remaining 34 varieties.  Seventeen varieties were found to have slow rusting resistance.  The resistance sources identified in this study can be used as resources for resistance against leaf rust in wheat breeding programs in China and the respective foreign countries.
Keywords:  wheat        gene postulation        molecular marker        Lr gene  
Received: 19 January 2020   Accepted:
Fund: This study was supported by the National Key Research and Development Program of China (2017YFD0300906-07).
Corresponding Authors:  Correspondence LI Zai-feng, E-mail:; LIU Da-qun, E-mail:    
About author:  LIU Yuan, E-mail:;

Cite this article: 

LIU Yuan, Takele Weldu GEBREWAHID, ZHANG Pei-pei, LI Zai-feng, LIU Da-qun. 2021. Identification of leaf rust resistance genes in common wheat varieties from China and foreign countries. Journal of Integrative Agriculture, 20(5): 1302-1313.

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