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Special Issue:
油料作物合辑Oil Crops
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| Development and characterization of new allohexaploid resistant to web blotch in peanut |
WANG Si-yu1, 2*, LI Li-na2*, FU Liu-yang1, 2*, LIU Hua2, QIN Li2, CUI Cai-hong2, MIAO Li-juan2, ZHANG Zhong-xin2, GAO Wei2, DONG Wen-zhao2, HUANG Bing-yan2, ZHENG Zheng2, TANG Feng-shou2, ZHANG Xin-you1, 2, DU Pei2 |
1 School of Life Sciences, Zhengzhou University, Zhengzhou 450001, P.R.China
2 Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou 450002, P.R.China |
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摘要
花生病害严重威胁花生生产，而通过种间杂交创制抗病材料是解决这一问题的有效途径。本研究利用花生栽培品种四粒红与野生种Arachis duranensis杂交，通过胚拯救和组织培养获得了种间杂种F1幼苗，细胞学和分子标记鉴定表明种间杂种F1为真杂种。进一步对扩繁F1幼苗进行秋水仙素处理，获得了F1种子，命名为Am1210。通过寡核苷酸荧光原位杂交鉴定、分子标记鉴定、表型鉴定和网斑病鉴定，我们发现：1）Am1210是Slh和ZW55种间杂交异源六倍体花生；2）蔓生、单粒或二粒荚果和红色种皮等性状相对于直立型、多粒荚果和褐色种皮为显性性状；3）Am1210的网斑病抗性与Slh相比显著提高，表明这种抗性来自于A. duranensis。此外，本研究还开发了69个显性和共显性分子标记，可用于种间杂种鉴定及未来A. duranensis染色体片段易位或渗入系的鉴定。
Abstract Peanut diseases seriously threaten peanut production, creating disease-resistant materials via interspecific hybridization is an effective way to deal with this problem. In this study, the embryo of an interspecific F1 hybrid was obtained by crossing the Silihong (Slh) cultivar with Arachis duranensis (ZW55), a diploid wild species. Seedlings were generated by embryo rescue and tissue culture. A true interspecific hybrid was then confirmed by cytological methods and molecular markers. After treating seedlings with colchicine during in vitro multiplication, the established interspecific F1 hybrid produced seeds which were named as Am1210. With oligonucleotide fluorescence in situ hybridization (Oligo FISH), molecular marker evaluations, morphological and web blotch resistance characterization, we found that: 1) Am1210 was an allohexaploid between Slh and ZW55; 2) the traits of spreading lateral branches, single-seeded or double-seeded pods and red seed coats were observed to be dominant compared to the erect type, multiple-seeded pods and brown seed coats; 3) the web blotch resistance of Am1210 was significantly improved than that of Slh, indicating the contribution of the web blotch resistance from the wild parent A. duranensis. In addition, 69 dominant and co-dominant molecular markers were developed which could be both used to verify the hybrid in this study and to identify translocation or introgression lines with A. duranensis chromosome fragments in future studies as well.
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Received: 15 October 2019
Accepted:
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| Fund: This research was supported by the National Natural Science Foundation of China (31801397), the Henan Province Young Talents Lifting Project, China (2018HYTP003), the Independent Innovation Foundation of Henan Academy of Agricultural Sciences, China (2019ZC13), the earmarked fund for China Agriculture Research System (CARS-13), and the Henan Provincial Agriculture Research System, China (S2012-05). |
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Corresponding Authors:
Correspondence ZHANG Xin-you, Tel: +86-371-65729560, E-mail: haasz@126.com; DU Pei, Tel: +86-371-61317913, E-mail: dupei2005@163.com.
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| About author: Wang Si-yu, E-mail: 15537323216@163.com; * These authors contributed equally to this study. |
Cite this article:
WANG Si-yu, LI Li-na, FU Liu-yang, LIU Hua, QIN Li, CUI Cai-hong, MIAO Li-juan, ZHANG Zhong-xin, GAO Wei, DONG Wen-zhao, HUANG Bing-yan, ZHENG Zheng, TANG Feng-shou, ZHANG Xin-you, DU Pei .
2021.
Development and characterization of new allohexaploid resistant to web blotch in peanut. Journal of Integrative Agriculture, 20(1): 55-64.
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