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Journal of Integrative Agriculture  2015, Vol. 14 Issue (7): 1321-1331    DOI: 10.1016/S2095-3119(14)60999-0
Physiology·Biochemistry·Cultivation·Tillage Advanced Online Publication | Current Issue | Archive | Adv Search |
Ethylene-inhibiting compound 1-MCP delays leaf senescence in cotton plants under abiotic stress conditions
 CHEN Yuan, J T Cothren, CHEN De-hua, Amir M H Ibrahim, Leonardo Lombardini
1、Department of Soil and Crop Sciences, Texas A&M University, College Station 77840, USA
2、Jiangsu Provincial Key Laboratory of Crops Genetics and Physiology, Yangzhou University, Yangzhou 100044, P.R.China
3、Department of Horticultural Sciences, Texas A&M University, College Station 77840, USA
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摘要  Cotton (Gossypium hirsutum L.) plants produce more ethylene when subjected to abiotic stresses, such as high temperatures and drought, which result in premature leaf senescence, reduced photosynthetic efficiency, and thus decreased yield. This study was conducted to test the hypothesis that the ethylene-inhibiting compound 1-methylcyclopropene (1-MCP) treatment of cotton plants can delay leaf senescence under high temperature, drought, and the aging process in controlled environmental conditions. Potted cotton plants were exposed to 1-MCP treatment at the early square stage of development. The protective effect of 1-MCP against membrane damage was found on older compared to younger leaves, indicating 1-MCP could lower the stress level caused by aging. Application of 1-MCP resulted in reduction of lipid peroxidation, membrane leakage, soluble sugar content, and increased chlorophyll content, in contrast to the untreated plants under heat stress, suggesting that 1-MCP treatment of cotton plants may also have the potential to reduce the effect of heat stress in terms of delayed senescence. Application of 1-MCP caused reductions of lipid peroxidation, membrane leakage, and soluble sugar content, together with increases in water use efficiency (WUE), water potential, chlorophyll content, and fluorescence quantum efficiency, compared to the untreated plants under drought, suggesting that 1-MCP treatment of cotton plants may also have the ability to reduce the level of stress under drought conditions. In conclusion, 1-MCP treatment of cotton should have the potential to delay senescence under heat and drought stress, and the aging process. Additionally, 1-MCP is more effective under stress than under non-stress conditions.

Abstract  Cotton (Gossypium hirsutum L.) plants produce more ethylene when subjected to abiotic stresses, such as high temperatures and drought, which result in premature leaf senescence, reduced photosynthetic efficiency, and thus decreased yield. This study was conducted to test the hypothesis that the ethylene-inhibiting compound 1-methylcyclopropene (1-MCP) treatment of cotton plants can delay leaf senescence under high temperature, drought, and the aging process in controlled environmental conditions. Potted cotton plants were exposed to 1-MCP treatment at the early square stage of development. The protective effect of 1-MCP against membrane damage was found on older compared to younger leaves, indicating 1-MCP could lower the stress level caused by aging. Application of 1-MCP resulted in reduction of lipid peroxidation, membrane leakage, soluble sugar content, and increased chlorophyll content, in contrast to the untreated plants under heat stress, suggesting that 1-MCP treatment of cotton plants may also have the potential to reduce the effect of heat stress in terms of delayed senescence. Application of 1-MCP caused reductions of lipid peroxidation, membrane leakage, and soluble sugar content, together with increases in water use efficiency (WUE), water potential, chlorophyll content, and fluorescence quantum efficiency, compared to the untreated plants under drought, suggesting that 1-MCP treatment of cotton plants may also have the ability to reduce the level of stress under drought conditions. In conclusion, 1-MCP treatment of cotton should have the potential to delay senescence under heat and drought stress, and the aging process. Additionally, 1-MCP is more effective under stress than under non-stress conditions.
Keywords:  1-MCP       ethylene       membrane leakage       lipid peroxidation       chlorophyll fluorescence       total soluble sugar  
Received: 22 July 2014   Accepted:
Fund: 

We are grateful for the financial support from AgroFresh (Yakima, WA, 98901, USA) and the National Natural Science Foundation of China (31171479 and 31471435).

Corresponding Authors:  CHEN Yuan, Tel: +1-9794224931; Fax: +1-9798450456; E-mail: chenyua3@tamu.edu     E-mail:  chenyua3@tamu.edu

Cite this article: 

CHEN Yuan, J T Cothren, CHEN De-hua, Amir M H Ibrahim, Leonardo Lombardini . 2015. Ethylene-inhibiting compound 1-MCP delays leaf senescence in cotton plants under abiotic stress conditions. Journal of Integrative Agriculture, 14(7): 1321-1331.

Asada K. 2006. Production and scavenging of reactive oxygenspecies in chloroplasts and their functions. Plant Physiology, 141, 391-396

Baker N R. 1991. A possible role for photosystem II inenvironmental perturbations of photosynthesis. PhysiologiaPlantarum, 81, 563-570

Bandurska H, Gniazdowska-Skoczek H. 1995. Cell membranestability in two barley genotypes under water stresscondisitons. Acta Societatis Botanicorum Poloniae, 64,29-32

Blankenship S M, Dole J M. 2003. 1-methylcyclopropene: Areview. Postharvest Biology and Technology, 28, 1-25

Bleecker A B, Kende H. 2000. Ethylene: A gaseoussignal molecule in plants. Annual Review of Cell AndDevelopmental Biology, 16, 1-18

Bleecker A B, Patterson S E. 1997. Last exit: Senescence,abscission, and meristem arrest in Arabidopsis. The PlantCell, 9, 1169-1179

Boote K J, Ibrahim A M H, Lafitte R, McCulley R, MessinaC, Murray S C, Giese J H. 2011. Position statement oncrop adaptation to climate change. Crop Science, 51,2337-2343

Burke J J, Mahan J R, Hatfield J L. 1988. Crop-specific thermalkinetic windows in relation to wheat and cotton biomassproduction. Agronomy Journal, 80, 553-556

Constable G A, Rawson H M. 1980. Carbon production andutilization in cotton: Inferences from a carbon budget.Australian Journal of Plant Physiology, 7, 539-553

da Costa V A, Cothren J T. 2011. Drought effects on gas exchange,chlorophyll, and plant growth of 1-methylcyclopropenetreated cotton. Agronomy Journal, 103, 1230-1241

Djanaguiraman M, Prasad P V V, Al-Khatib K. 2011. Ethyleneperception inhibitor 1-MCP decreases oxidative damage ofleaves through enhanced antioxidant defense mechanismsin soybean plants grown under high temperature stress.Environmental and Experimental Botany, 71, 215-223

Dong H, Li W, Tang W, Li Z, Zhang D, Niu Y. 2006. Yield, qualityand leaf senescence of cotton grown at varying plantingdates and plant densities in the Yellow River Valley of China.Field Crops Research, 98, 106-115

Dong H, Mao S, Zhang W, Chen D. 2014. On boll-settingoptimization theory for cotton cultivation and its newdevelopment. Scientia Agricultura Sinica, 47, 441-451

(in Chinese)Dong H, Niu Y, Kong X, Luo Z. 2009. Effects of early-fruitremoval on endogenous cytokinins and abscisic acidin relation to leaf senescence in cotton. Plant GrowthRegulation, 59, 93-101

Edreva A. 2005. Generation and scavenging of reactiveoxygen species in chloroplasts: A submolecular approach.Agriculture, Ecosystems & Environment, 106, 119-133

Faragher J D, Wachtel E, Mayak S. 1987. Changes in thephysical state of membrane lipids during senescence ofrose petals. Plant Physiology, 83, 1037-1042

Gan S, Amasino R M. 1997. Making sense of senescence(molecular genetic regulation and manipulation of leafsenescence). Plant Physiology, 113, 313-319

Genkov T, Tsoneva P, Ivanova I. 1997. Effect of cytokininson photosynthetic pigments and chlorophyllase activity inin vitro cultures of axillary buds of dianthus caryophyllus l.Journal of Plant Growth Regulation, 16, 169-172

Gepstein S, Thimann K V. 1981. The role of ethylene in thesenescence of oat leaves. Plant Physiology, 68, 349-354

Giardi M T, Cona A, Geiken B, Kucera T, Masojidek J, MattooA K. 1996. Long-term drought stress induces structuraland functional reorganization of photosystem II. Planta,199, 118-125

Grbi? V, Bleecker A B. 1995. Ethylene regulates the timingof leaf senescence in Arabidopsis. The Plant Journal, 8,595-602

Hake K, Carter F, Mauney J, Namken N, Heitholt J, Kerby T,Pettigrew B. 1992. Sqaure retention. Cotton PhsiologyToday, 3.Havaux M. 1992. Stress tolerance of photosystem II in vivo:Antagonistic effects of water, heat, and photoinhibitionstresses. Plant Physiology, 100, 424-432

Hodge J E, Hofereiter B T. 1962. Determination of reducingsugars and carbohydrates. In: Whistler R L, Wolfrom J L,eds., Methods in Carbohydrate Chemistry. Acedemic Press,New York. pp. 380-394

Jiang W, Sheng Q, Zhou X J, Zhang M J, Liu X J. 2002.Regulation of detached coriander leaf senescence by1-methylcyclopropene and ethylene. Postharvest Biologyand Technology, 26, 339-345

Kao C H, Yang S F. 1983. Role of ethylene in the senescenceof detached rice leaves. Plant Physiology, 73, 881-885

Kawakami E M, Oosterhuis D, Snider J. 2010. Physiologicaleffects of 1-methylcyclopropene on well-watered and waterstressedcotton plants. Journal of Plant Growth Regulation,29, 280-288

Lieberman M. 1979. Biosynthesis and action of ethylene. AnnualReview of Plant Physiology and Plant Molecular Biology,30, 533-591

Lim P O, Kim H J, Nam H G. 2007. Leaf senescence. AnnualReview of Plant Biology, 58, 115-136

Liu W J, Yuan S, Zhang N H, Lei T, Duan H G, Liang H G, LinH H. 2006. Effect of water stress on photosystem 2 in twowheat cultivars. Biologia Plantarum, 50, 597-602

Liu X Z, Huang B R. 2000. Heat stress injury in relation tomembrane lipid peroxidation in creeping bentgrass. CropScience, 40, 503-510

Martineau J R, Specht J E, Williams J H, Sullivan C Y. 1979.Temperature tolerance in soybeans. I. Evaluation of atechnique for assessing cellular membrane thermostability.Crop Science, 19, 75-78

McWilliams D. 2003. Drought Strategies for Cotton. CooperativeExtension Service, Circular 582, College of Agriculture andHome Economics, New Mexico State University.Morgan P W, Durham J I. 1973. Leaf age and ethylene-inducedabscission. Plant Physiology, 52, 667-670

Pettigrew W T. 2004. Physiological consequences of moisturedeficit stress in cotton. Crop Science, 44, 1265-1272

Pettigrew W T. 2008. The effect of higher temperatures oncotton lint yield production and fiber quality. Crop Science, 48, 278-285

Pierik R, Sasidharan R, Voesenek L. 2007. Growth control byethylene: Adjusting phenotypes to the environment. Journalof Plant Growth Regulation, 26, 188-200

Premachandra G S, Shimada T. 1987. The measurement ofcell-membrane stability using polyethylene-glycol as adrought tolerance-test in wheat. Japanese Journal of CropScience, 56, 92-98

Reddy K R, Hodges H F, Reddy V R. 1992. Temperature effectson cotton fruit retention. Agronomy Journal, 84, 26-30

Sekhon R S, Childs K L, Santoro N, Foster C E, Buell C R, deLeon N, Kaeppler S M. 2012. Transcriptional and metabolicanalysis of senescence induced by preventing pollinationin maize. Plant Physiology, 159, 1730-1744

Sisler E C, Dupille E, Serek M. 1996. Effect of1-methylcyclopropene and methylenecyclopropane onethylene binding and ethylene action on cut carnations.Plant Growth Regulation, 18, 79-86

Sisler E C, Serek M. 1997. Inhibitors of ethylene responsesin plants at the receptor level: Recent developments.Physiologia Plantarum, 100, 577-582

Snider J L, Oosterhuis D M, Kawakami E M. 2010. Genotypicdifferences in thermotolerance are dependent uponprestress capacity for antioxidant protection of thephotosynthetic apparatus in Gossypium hirsutum.Physiologia Plantarum, 138, 268-277

Suttle J C, Hultstrand J F. 1991. Ethylene-induced leafabscission in cotton seedlings: the physiological bases forage-dependent differences in sensitivity. Plant Physiology,95, 29-33

Suttle J C, Kende H. 1980. Ethylene action and loss of membraneintegrity during petal senescence in Tradescantia. PlantPhysiology, 65, 1067-1072

Thompson J E, Mayak S, Shinitzky M, Halevy A H. 1982.Acceleration of membrane senescence in cut carnationflowers by treatment with ethylene. Plant Physiology, 69,859-863

Wills R B H, Ku V V V, Warton M A. 2002. Use of1-methylcyclopropene to extend the postharvest life oflettuce. Journal of the Science of Food and Agriculture,82, 1253-1255

Wingler A, Masclaux-Daubresse C, Fischer A M. 2009. Sugars,senescence, and ageing in plants and heterotrophicorganisms. Journal of Experimental Botany, 60, 1063-1066

Wingler A, Purdy S, MacLean J A, Pourtau N. 2006. The roleof sugars in integrating environmental signals during theregulation of leaf senescence. Journal of ExperimentalBotany, 57, 391-399

Wright P R. 1999. Premature senescence of cotton (Gossypiumhirsutum L.)-predominantly a potassium disorder causedby an imbalance of source and sink. Plant Soil, 211,231-239

Wullschleger S D, Oosterhuis D M. 1990. Photosynthetic carbonproduction and use by developing cotton leaves and bolls.Crop Science, 30, 1259-1264

Yan K, Chen P, Shao H, Zhao S, Zhang L, Xu G, Sun J.2012. Responses of photosynthesis and photosystem II tohigher temperature and salt stress in sorghum. Journal ofAgronomy and Crop Science, 198, 218-226

Yang G, Rhodes D, Joly R. 1996. Effects of high temperatureon membrane stability and chlorophyll fluorescence inglycinebetaine-deficient and glycinebetaine-containingmaize lines. Australian Journal of Plant Physiology, 23,437-443

Young T E, Meeley R B, Gallie D R. 2004. Acc synthaseexpression regulates leaf performance and droughttolerance in maize. Plant Journal, 40, 813-825

Zhao D, Reddy K R, Kakani V G, Koti S, Gao W. 2005.Physiological causes of cotton fruit abscission underconditions of high temperature and enhanced ultraviolet-Bradiation. Physiologia Plantarum, 124, 189-199
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