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Journal of Integrative Agriculture  2020, Vol. 19 Issue (10): 2407-2418    DOI: 10.1016/S2095-3119(19)62873-X
Special Issue: 麦类耕作栽培合辑Triticeae Crops Physiology · Biochemistry · Cultivation · Tillage
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Yield-related agronomic traits evaluation for hybrid wheat and relations of ethylene and polyamines biosynthesis to filling at the mid-grain filling stage
YANG Wei-bing, QIN Zhi-lie, SUN Hui, LIAO Xiang-zheng, GAO Jian-gang, WANG Yong-bo, HOU Qi-ling, CHEN Xian-chao, TIAN Li-ping, ZHANG li-ping, MA Jin-xiu, CHEN Zhao-bo, ZHANG Feng-ting, ZHAO Chang-ping
Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, P.R.China
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Abstract  Because of the yield increase of 3.5–15% compared to conventional wheat, hybrid wheat is considered to be one of the main ways to greatly improve the wheat yield in the future.  In this study, we performed a principal component analysis (PCA) on two-line hybrids wheat and their parents using the grain weight (GW), the length of spike (LS), the kernel number of spike (KSN), and spike number (SPN) as variables.  The results showed that the variables could be classified into three main factors, the weight factor (factor 1), the quantity factor 1 (factor 2) and the quantity factor 2 (factor 3), which accounted for 37.1, 22.6 and 18.5%, respectively of the total variance in different agronomic variables, suggesting that the GW is an important indicator for evaluating hybrid combinations, and the grain weight of restorer line (RGW) could be used as a reference for parents selection.  The hybrid combination with a higher score in factor 1 direction and larger mid-parent heterosis (MPH) of the GW and its parents were used to carry out the analysis of grain filling, 1-aminocylopropane-1-carboxylicacid (ACC) and polyamine synthesis related genes.  The results suggested that the GW of superior grain was significantly higher than that of inferior grains in BS1453×JS1 (H) and its parents.  Both grain types showed a weight of H between BS1453 (M) and JS1(R), and a larger MPH, which may be caused by their differences in the active filling stage and the grain filling rate.  The ADP-glucose pyrophosphorylase (AGPase), granule bound starch synthase I (GBSSI), starch synthase III (SSS), and starch branching enzyme-I (SBE-I) expression levels of H were intermediated between M and R, which might be closely related to MPH formation of the GW.  Compared with R and H, the GW of M at maturity was the lowest.  The expression levels of spermidine synthase 2 (Spd2), ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (SAMDC) had significantly positive correlations with the grain filling rate (r=0.77*, 0.51*, 0.59*), suggesting their major roles in the grain filling and heterosis formation.  These provide a theoretical basis for improving the GW of photo-thermo-sensitive male sterile lines (PTSMSL) by changing the endogenous polyamine synthesis in commercial applications.
Keywords:  hybrid wheat        agronomic traits        ethylene        polyamines        grain filling        heterosis  
Received: 25 June 2019   Accepted:
Fund: This work was supported by the Beijing Natural Science Foundation, China (6194035), the Youth Fund Project from Beijing Academy of Agricultural and Forestry Sciences, China (QNJJ201629) and the Beijing Excellent Talents Training Funding, China (2017000020060G130).
Corresponding Authors:  Correspondence ZHAO Chang-ping, Tel: +86-10-51503918, E-mail:; ZHANG Feng-ting, Tel: +86-10-51503104, E-mail:   
About author:  YANG Wei-bing, E-mail:;

Cite this article: 

YANG Wei-bing, QIN Zhi-lie, SUN Hui, LIAO Xiang-zheng, GAO Jian-gang, WANG Yong-bo, HOU Qi-ling, CHEN Xian-chao, TIAN Li-ping, ZHANG li-ping, MA Jin-xiu, CHEN Zhao-bo, ZHANG Feng-ting, ZHAO Chang-ping. 2020. Yield-related agronomic traits evaluation for hybrid wheat and relations of ethylene and polyamines biosynthesis to filling at the mid-grain filling stage. Journal of Integrative Agriculture, 19(10): 2407-2418.

Akel W, Rapp M, Thorwarth P, Würschum T, Longin C. 2019. Hybrid durum wheat: Heterosis of grain yield and quality traits and genetic architecture of anther extrusion. Theoretical and Applied Genetics, 132, 921–932.
Chen T T, Xu Y J, Wang J C, Wang Z Q, Yang J C, Zhang J H. 2013. Polyamines and ethylene interact in rice grains in response to soil drying during grain filling. Journal of Experimental Botany, 64, 2523–2538.
DiTomaso J M, Shaff J E, Kochian L V. 1989. Putrescine-induced wounding and its effects on membrane integrity and ion transport processes in roots of intact corn seedlings. Plant Physiology, 90, 988–995.
Feng H Y, Wang Z M, Kong F N, Zhang M J, Zhou S L. 2011. Roles of carbohydrate supply and ethylene, polyamines in maize kernel set. Journal of Integrative Plant Biology, 53, 388–398.
Ferrante A, Cartelle J, Savin R, Slafer G A. 2017. Yield determination, interplay between major components and yield stability in a traditional and a contemporary wheat across a wide range of environments. Field Crops Research, 203, 114–127.
Fischer R A, Stapper M. 1987. Lodging effects on high yielding crops of irrigated semi-dwarf wheat. Field Crops Research, 17, 245–258.
Fischer S, Maurer H P, Würschum T, Möhring J, Piepho H P, Schön C C, Thiemt E M, Dhillo B S, Weissmann E A, Melchinger A E, Reif J C. 2010. Development of heterotic groups in triticale. Crop Science, 50, 584–590.
Gai J Y, Dai J R, Li J S, Sun Q X. 2016. Utilization of Heterosis in Crops. High Education Press, Beijing, China. (in Chinese)
Garcia A A, Wang S, Melchinger A E, Zeng Z B. 2008. Quantitative trait loci mapping and the genetic basis of heterosis in maize and rice. Genetics, 180, 1707–1724.
Gardiner D M, Kazan K M, Praud S, Torney F J, Rusu A, Manners J M. 2010. Early activation of wheat polyamine biosynthesis during Fusarium head blight implicates putrescine as an inducer of trichothecene mycotoxin production. BMC Plant Biology, 10, 289–292.
Gilbert J, Procunier J, Aung T. 2000. Influence of the D genome in conferring resistance to Fusarium head blight in spring wheat. Euphytica, 114, 181–186.
Hurkman W J, McCue K F, Altenbach S B, Korn A, Tanaka C K, Kothari K M, Johnson E L, Bechtel D B, Wilson J D, Anderson O D. 2003. Effect of temperature on expression of genes encoding enzymes for starch biosynthesis in developing wheat endosperm. Plant Science, 164, 873–881.
Keeling P L, Bacon P J, Holt D C. 1993. Elevated temperature reduces starch deposition in wheat endosperm by reducing the activity of soluble starch synthase. Planta, 191, 342–348.
Kuehn G D, Phillips G C. 2005. Role of polyamines in apoptosis and other recent advances in plant polyamines. Critical Reviews in Plant Sciences, 24, 123–130.
Li L, Lu K, Chen Z, Mou T, Hu Z. 2008. Dominance, overdominance and epistasis condition the heterosis in two heterotic rice hybrids. Genetics, 180, 1725–1742.
Liu D, Hu S, Yu J, Zhang A. 2011. Comparative analysis of mitochondrial genomes between a wheat K-type cytoplasmic male sterility (CMS) line and its maintainer line. BMC Genomics, 12, 163.
Liu Z Y, Sun N N, Ba X B, Li L Z, Xin Q G, Yu J C. 2015. Discussion on strategies for wheat cross combination and assortative mating. Shandong Agricultural Sciences, 47, 17–24. (in Chinese)
Longin C, Mühleisen J, Maurer H,  Zhang H L, Manje Gowda M, Reif J. 2012. Hybrid breeding in autogamous cereals. Theoretical and Applied Genetics, 125, 1087–1096.
Lu Z M, Xu B Q. 2010. On significance of heterotic group theory in hybrid rice breeding. Rice Science, 17, 94–98.
Morgan P W, Drew M C. 1997. Ethylene and plant response to stress. Physiologia Plant, 100, 620–630.
Narayana I, Lalonde S, Saini H S. 1991. Water-stress induced ethylene production in wheat. Plant Physiology, 96, 406–410.
Ni F, Qi J, Hao Q, Lyu B, Luo M C, Wang Y, Chen F, Wang S, Zhang C, Epstein L, Zhao X, Wang H, Zhang X, Chen C, Sun L, Fu D. 2017. Wheat Ms2 encodes for an orphan protein that confers male sterility in grass species. Nature Communications, 8, 15121.
Richards F J. 1959. A flexible growth function for empirical use. Journal of Experimental Botany, 10, 290–301.
Rook F, Corke F, Card R, Munz G, Smith C, Bevan M W. 2001. Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signaling. The Plant Journal, 26, 421–433.
Royo C, Alvaro F, Martos V, Ramdani A, Isidro J, Villegas D. 2007. Genetic changes in durum wheat yield components and associated traits in Italian and Spanish varieties during the 20th century. Euphytica, 155, 259–270.
Ryan W, Delphine F, Jochen C, Reif M G, Takashi O, Viktor K, Peter L. 2013. Hybrid breeding in wheat: Technologies to improve hybrid wheat seed production. Journal of Experimental Botany, 64, 5411–5428.
Sanchez-Garcia M, Royo C, Aparicio N, Martin-Sanchez J A, Alvaro F. 2013. Genetic improvement of bread wheat yield and associated traits in Spain during the 20th century. Journal of Agricultural Science, 151, 105–118.
Serrago R A, Alzueta I, Savin R, Slafer G A. 2013. Understanding grain yield responses to source-sink ratios during grain filling in wheat and barley under contrasting environments. Field Crops Research, 150, 42–51.
Shi M F, An L L, Ding G Q, Xing C P, Han D C. 2012. Combining ability evaluation and genetic analysis on wheat agronomic traits involving parents with dwarf and large spike and varieties with multi-resistant and high yield. Chinese Agricultural Science Bulletin, 28, 50–56. (in Chinese)
Song G S, Zhai H L, Peng Y G, Zhang L, Wei G. 2010. Comparative transcriptional profiling and preliminary study on heterosis mechanism of super-hybrid rice. Molecular Plant, 3, 1012–1025.
Subramaniam K, Abbo S, Ueng P P. 1996. Isolation of two differentially expressed wheat ACC synthase cDNA and the characterization of one of their genes with root-predominant expression. Plant Molecular Biology, 31, 1009–1020.
Tucker E J, Baumann U, Kouidri A, Suchecki R, Mathieu B, Garcia M, Okada T, Dong C M, Wu Y Z, Sandhu A, Singh M, Langridge P, Wolters P, Albertsen M C, Cigan A M, Whitford R. 2017. Molecular identification of the wheat male fertility gene Ms1 and its prospects for hybrid breeding. Nature Communications, 8, 869.
Wang K, Qiu F L, Larazo W, Paz M A, Xie F M. 2015. Heterotic groups of tropical indica rice germplasm. Theoretical and Applied Genetics, 128, 421–430.
Wang Z, Li J, Chen S X, Heng Y F, Chen Z, Yang J, Zhou K J, Pei J W, He H, Deng X W, Ma L G. 2017. Poaceae-specific MS1 encodes a phospholipid-binding protein for male fertility in bread wheat. Proceedings of the National Academy of Sciences of the United States of America, 114, 12614–12619.
Wang Z, Yin Y, He M, Zhang Y, Lu S, Li Q, Shi S. 2003. Allocation of photosynthates and grain growth of two wheat cultivars with different potential grain growth in response to pre- and post-anthesis shading. Journal of Agronomy and Crop Science, 189, 280–285.
Wang Z Q, Xu Y J, Wang J C, Yang J C. 2012. Polyamine and ethylene interactions in grain filling of superior and inferior spikelets of rice. Plant Growth Regulation, 66, 215–228.
Yan S, Li W Y, Yin Y P, Wang Z L. 2010. Sink strength in relation to growth of superior and inferior grains within a wheat spike. Journal of Agricultural Science, 148, 567–578.
Yang J, Zhang J, Wang Z, Zhu Q, Liu L. 2001. Water deficit-induced senescence and its relationship to the remobilization of pre-stored carbon in wheat during grain filling. Agronomy Journal, 93, 196–206.
Yang W B, Li Y X, Yin Y P, Qin Z L, Zheng M J, Chen J, Luo Y L, Pang D W, Jiang W W, Li Y, Wang Z L. 2017. Involvement of ethylene and polyamines biosynthesis and abdominal phloem tissues characters of wheat caryopsis during grain filling under stress conditions. Scientific Reports, 7, 46020.
Yang W B, Yin Y P, Li Y, Cai T, Ni Y L, Peng D L, Wang Z L. 2014. Interactions between polyamines and ethylene during grain filling in wheat grown under water deficit conditions. Plant Growth Regulation, 72, 189–201.
Zadoks J C, Chang T T, Konzak C F. 1974. A decimal code for the growth stages of cereals. Weed Research, 14, 415–421.
Zhao H, Yang H. 2008. Exogenous polyamines alleviate the lipid peroxidation induced by cadmium chloride stress in Malus hupehensis Rehd. Scientia Horticulturae, 116, 442–447.
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