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Antagonizing Aspergillus parasiticus and Promoting Peanut Growth of Bacillus Isolated from Peanut Geocarposphere Soil |
XIAO Wei, YAN Pei-sheng, WU Han-qi and LIN Feng |
School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, P.R.China |
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摘要 A set of 38 Bacillus strains isolated from peanut geocarposphere soil were screened as potential biological control agent anti- Aspergillus parasiticus. Tip-culture method with rapid and quantitative characteristics was used to determine anti-A. parasiticus activity and the process of isolation could be accelerated with this method. 12 out of 38 Bacillus strains showed high anti-aflatoxin production activity. These 12 Bacillus strains were selected to identify the characteristics of promoting peanuts germination rate. Pot experiment in greenhouse was carried out by using these strains which can promote peanuts germination. Phytohormones in the fermentation broth were also detected as another important reference factor to select the isolates as biological control agent with PGPR features. These Bacillus strains isolated from peanut geocarposphere soil not only had high ability anti-Aspergillus parasiticus, but also promoted peanut growth. Therefore, these Bacillus strains were well adapted to peanut production in the field as biological control agent with plant growth promoting rhizobacteria (PGPR) features.
Abstract A set of 38 Bacillus strains isolated from peanut geocarposphere soil were screened as potential biological control agent anti- Aspergillus parasiticus. Tip-culture method with rapid and quantitative characteristics was used to determine anti-A. parasiticus activity and the process of isolation could be accelerated with this method. 12 out of 38 Bacillus strains showed high anti-aflatoxin production activity. These 12 Bacillus strains were selected to identify the characteristics of promoting peanuts germination rate. Pot experiment in greenhouse was carried out by using these strains which can promote peanuts germination. Phytohormones in the fermentation broth were also detected as another important reference factor to select the isolates as biological control agent with PGPR features. These Bacillus strains isolated from peanut geocarposphere soil not only had high ability anti-Aspergillus parasiticus, but also promoted peanut growth. Therefore, these Bacillus strains were well adapted to peanut production in the field as biological control agent with plant growth promoting rhizobacteria (PGPR) features.
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Received: 09 June 2013
Accepted:
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Fund: This study was supported by the National Natural Science Foundation of China (30571244, 30870003), and the National Key Technology R&D Program of China (2009BADA0B05-4). |
Corresponding Authors:
YAN Pei-sheng, E-mail: psyan6@hotmail.com
E-mail: psyan6@hotmail.com
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About author: XIAO Wei, E-mail: tuerxiao@yeah.net |
Cite this article:
XIAO Wei, YAN Pei-sheng, WU Han-qi and LIN Feng.
2014.
Antagonizing Aspergillus parasiticus and Promoting Peanut Growth of Bacillus Isolated from Peanut Geocarposphere Soil. Journal of Integrative Agriculture, 13(11): 2445-2451.
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