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Journal of Integrative Agriculture  2014, Vol. 13 Issue (11): 2445-2451    DOI: 10.1016/S2095-3119(13)60609-7
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Antagonizing Aspergillus parasiticus and Promoting Peanut Growth of Bacillus Isolated from Peanut Geocarposphere Soil
 XIAO Wei, YAN Pei-sheng, WU Han-qi and LIN Feng
School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, P.R.China
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摘要  A set of 38 Bacillus strains isolated from peanut geocarposphere soil were screened as potential biological control agent anti- Aspergillus parasiticus. Tip-culture method with rapid and quantitative characteristics was used to determine anti-A. parasiticus activity and the process of isolation could be accelerated with this method. 12 out of 38 Bacillus strains showed high anti-aflatoxin production activity. These 12 Bacillus strains were selected to identify the characteristics of promoting peanuts germination rate. Pot experiment in greenhouse was carried out by using these strains which can promote peanuts germination. Phytohormones in the fermentation broth were also detected as another important reference factor to select the isolates as biological control agent with PGPR features. These Bacillus strains isolated from peanut geocarposphere soil not only had high ability anti-Aspergillus parasiticus, but also promoted peanut growth. Therefore, these Bacillus strains were well adapted to peanut production in the field as biological control agent with plant growth promoting rhizobacteria (PGPR) features.

Abstract  A set of 38 Bacillus strains isolated from peanut geocarposphere soil were screened as potential biological control agent anti- Aspergillus parasiticus. Tip-culture method with rapid and quantitative characteristics was used to determine anti-A. parasiticus activity and the process of isolation could be accelerated with this method. 12 out of 38 Bacillus strains showed high anti-aflatoxin production activity. These 12 Bacillus strains were selected to identify the characteristics of promoting peanuts germination rate. Pot experiment in greenhouse was carried out by using these strains which can promote peanuts germination. Phytohormones in the fermentation broth were also detected as another important reference factor to select the isolates as biological control agent with PGPR features. These Bacillus strains isolated from peanut geocarposphere soil not only had high ability anti-Aspergillus parasiticus, but also promoted peanut growth. Therefore, these Bacillus strains were well adapted to peanut production in the field as biological control agent with plant growth promoting rhizobacteria (PGPR) features.
Keywords:  Bacillus strains       anti-Aspergillus parasiticus       PGPR features       peanut  
Received: 09 June 2013   Accepted:
Fund: 

This study was supported by the National Natural Science Foundation of China (30571244, 30870003), and the National Key Technology R&D Program of China (2009BADA0B05-4).

Corresponding Authors:  YAN Pei-sheng, E-mail: psyan6@hotmail.com     E-mail:  psyan6@hotmail.com
About author:  XIAO Wei, E-mail: tuerxiao@yeah.net

Cite this article: 

XIAO Wei, YAN Pei-sheng, WU Han-qi and LIN Feng. 2014. Antagonizing Aspergillus parasiticus and Promoting Peanut Growth of Bacillus Isolated from Peanut Geocarposphere Soil. Journal of Integrative Agriculture, 13(11): 2445-2451.

Cao L H, Xu S C, Lin R M, Liu T G, Chen W Q. 2008. Earlymolecular diagnosis and detection of Puccinia striiformisf. sp. tritici in China. Letters in Applied Microbiology,46, 501-506

Chen H Q, Sui C, Wei J H. 2009. Summary of strategies fordeveloping SSR primer. Molecular Plant Breeding, 7,845-851

Gao L, Chen W, Liu T. 2010. Development of a SCAR markerby inter-simple sequence repeat for diagnosis of dwarf buntof wheat and detection of Tilletia controversa Kühn. FoliaMicrobiologica, 55, 258-264

Gao L, Chen W, Liu T. 2011. An ISSR-based approach forthe molecular detection and diagnosis of dwarf bunt ofwheat, caused by Tilletia controversa Kühn. Journal ofPhytopathology, 159, 155-158

Jak?e J, Javornik B. 2001. High throughput isolation ofmicrosatellites in hop (Humulus lupulus L.). PlantMolecular Biology Reporter, 19, 217-226

Jin Y, Pretorius Z A, Singh R P. 2007. New virulence within race TTKS (Ug99) of the stem rust pathogen and effectiveresistance genes. Phytopathology, 97, S137-S137.

Jin Y, Singh R P. 2006. Resistance in US wheat to recenteastern African isolates of Puccinia graminis f. sp. triticiwith virulence to resistance gene Sr31. Plant Disease, 90,476-480

Keiper F J, Haque M S, Hayden M J, Park R F. 2006. Geneticdiversity in Australian populations of Puccinia graminisf. sp. avenae. Phytopathology, 96, 96-104

Leonard K J, Szabo L J. 2005. Stem rust of small grains andgrasses caused by Puccinia graminis. Molecular PlantPathology, 6, 99-111

Liu J H, Gao L, Liu T G, Chen W Q. 2009. Developmentof a sequence-characterized amplified region marker fordiagnosis of dwarf bunt of wheat and detection of Tilletiacontroversa Kühn. Letters in Applied Microbiology, 49,235-240

Mackenzie D. 2007. Billions at risk from wheat super-blight.New Scientist, 193, 35.

Mottura M, Gailing O, Verga A, Finkeldey R. 2004. Efficiencyof microsatellite enrichment in Prosopis chilensis usingmagnetic capture. Plant Molecular Biology Reporter, 22,251-258

Shen F J, Phill W, Zhang Z H, Zhang A J, Stephanie S, Steve JK, Yue B S 2005. Enrichment of giant panda microsatellitemarkers using dynal magnet beads. Acta Genetica Sinica,32, 457-462 (in Chinese)

Szabo L J. 2007. Development of simple sequence repeatmarkers for the plant pathogenic rust fungus, Pucciniagraminis. Molecular Ecology Notes, 7, 92-94

Szabo L J, Kolmer J A. 2007. Development of simple sequencerepeat markers for the plant pathogenic rust fungusPuccinia triticina. Molecular Ecology Notes, 7, 708-710

Tsukasa N, Satomi N, Koji M, Hirotaka Y, Hiroyuki F. 2006.A protocol for the construction of microsatellite enrichedgenomic library. Plant Molecular Biology Reporter, 24,305-312

Visser B, Herselman L, Pretorius Z A. 2009. Geneticcomparison of Ug99 with selected South African races ofPuccinia graminis f.sp. tritici. Molecular Plant Pathology,10, 213-222

Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, HornesM, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. 1995.AFLP: A new technique for DNA fingerprinting. NucleicAcids Research, 23, 4407-4414

Wang X J, Zheng W M, Buchenauer H, Zhao J, Han Q M,Huang L L, Kang Z S. 2008. The development of a PCRbasedmethod for detecting Puccinia striiformis latentinfections in wheat leaves. European Journal of PlantPathology, 120, 241-247

Zambino P J. 2002. Dry grinding at near-ambient temperaturesfor extracting DNA from rust and other fungal spores.Biotechniques, 33, 48-51

Zane L, Bargelloni L, Patarnello T. 2002. Strategies formicrosatellite isolation: A review. Molecular Ecology,11, 1-16

Zhong S, Leng Y, Friesen T L, Faris J D, Szabo L J. 2009.Development and characterization of expressed sequencetag-derived microsatellite markers for the wheat stem rustfungus Puccinia graminis Brown R L, Cotty P J, Cleveland T E. 1991. Reductionin aflatoxin content of maize by atoxigenic strains ofAspergillus flavus. Journal of Food Protection, 54, 623-626

Cotty P J 1994. Influence of field application of an atoxigenicstrain of Aspergillus flavus on the populations of A.flavus infecting cotton bolls and on aflatoxin content ofcottonseed. Phytopathology, 84, 1270-1277

Dorner J W, Cole R J, Blankenship P D 1992. Use of abiocompetitive agent to control preharvest aflatoxin indrought stressed peanuts. Journal of Food Protection,55, 888-892

Dorner J W, Cole R J, Blankenship P D. 1998. Effect ofinoculum rate of biological control agents on preharvestaflatoxin contamination of peanuts. Biological Control,12, 171-176

Dorner J W, Cole R J, Wicklow D T 1999 Aflatoxin reductionin corn through field application of competitive fungi.Journal of Food Protection, 62, 650-656

Figueiredo M V, Martinez C R B, Burity H A, Chanway C P2008. Plant growth-promoting rhizobacteria for improvingnodulation and nitrogen fixation in the common bean(Phaseolus vulgaris L.). World Journal of Microbiology& Biotechnology, 24, 1187-1193

Kerry B R. 2000. Rhizosphere interactions and the exploitationof microbial agents for the biological control of plantparasiticnematodes. Annual Review of Phytopathology,38, 423-441

Kloepper J W, Schroth M N 1978. Plant growth promotingrhizobacteria on radishes. In: Proceedings of the VIthInternational Conference on Plant Pathogenic Bacteria.Angres, France, 2, 879-882

Kondo T, Sakurada M, Okamoto S, Ono M, Tsukigi H,Suzuki A, Nagasawa H, Sakuda S 2001. Effects ofaflastatin A, an inhibitor of aflatoxin production, onaflatoxin biosynthetic pathway and glucose metabolismin Aspergillus parasiticus. Journal of Antibiotics (Tokyo),54, 650-657

Malik K A, Rakhshanda B. 1997. Association of nitrogenfixingplant growth promoting rhizobateria (PGPR) withKallar grass and rice. Plant and Soil, 194, 37-44

Mickler C J, Bowen K L, Kloepper J K. 1995. Evaluation ofselected geocarposphere bacteria for biological control ofAspergillusflavus in peanut. Plant and Soil, 175, 291-299

Moyne A L, Shelby R, Cleveland T E, Tuzun S. 2001.Bacillomycin D: An iturin with antifungal activity againstAspergillus flavus. Journal of Applied Microbiology, 90,622-629

Paster N, Drody S, Chalutz E, Menasherov M, Nitzan R,Wilson C L. 1993. Evaluation of the potential of theyeast Pichia guilliermondii as a biological control agentagainst Aspergillus flavus and fungi of stored soya beans.Mycological Research, 97, 1201-1206

Taylor W J, Draughon F A. 2001. Nannocystis exedens: Apotential biocompetitive agent against Aspergillus flavusand Aspergillus parasiticus. Journal of Food Protection,64, 1030-1034

Tong J H, Li Y W, Huang Z G, Tian M, Ding J H. 2009.Simultaneous determination of several phytohormonesin cotton root by using high performance liquidchromatography. Progress in Modern Biomedicine, 9,2476-2479

Van L C. 2007. Plant responses to plant growth-promotingbacteria. European Journal of Plant Pathology, 119,243-254

Yabe K, Nakamura H, Ando Y, Terakado N, Nakajima H,Hamasaki T. 1988. Isolation and characterization ofAspergillus parasiticus mutants with impaired aflatoxinproduction by a novel tip culture method. Applied andEnvironmental Microbiology, 54, 2096-2100

Yan P S, Gao X J, Wu H Q, Li Q W, Ning L M, Guan S S.2010. Isolation and screening of biological control bacterialstrains against Aspergillus parassiticus from groundnutgeocarposphere. Journal of Earth Science, 21, 309-311

Yan P S, Song Y, Sakuno E, Nakajima H, Nakagawa H,Yabe K. 2004. Cyclo (L-leucyl-L-prolyl) produced byAchromobacter xylosoxidans inhibits aflatoxin productionby Aspergillus parasiticus. Appllied & EnvironmentalMicrobiology, 70, 7466-7473

f. sp. tritici. Phytopathology,99, 282-289
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