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Special Issue:
食品科学合辑Food Science
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| Protective effect of high-oleic acid peanut oil and extra-virgin olive oil in rats with diet-induced metabolic syndrome by regulating branched-chain amino acids metabolism |
| ZHAO Zhi-hao1, SHI Ai-min1, 2, GUO Rui1, LIU Hong-zhi1, 2, HU Hui1, WANG Qiang1, 2 |
1 Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, P.R.China
2 Collaborative Innovation Center for Modern Grain Circulation and Safety, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, P.R.China
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摘要
前期研究表明，高油酸花生油(HOPO)和特级初榨橄榄油(EVOO)对代谢综合征(MS)具有预防作用。本研究旨在评估HOPO和EVOO膳食干预预防MS的代谢效应，以及肠道菌群在其中的作用。选用Sprague-Dawley大鼠分别喂食正常饲料、高果糖-高脂肪饲料、含HOPO的高果糖-高脂肪饲料和含EVOO的高果糖-高脂肪饲料，持续12周。利用基于UPLC-Q/TOF-MS的非靶向代谢组学分析粪便和血清样品的代谢组学特征，采用偏最小二乘判别分析(PLS-DA)对组间粪便和血清中潜在生物标志物进行鉴定，评估肠道菌群与潜在生物标志物的相关性，并对血清生物标志物进行通路分析。结果表明：各组粪便和血清代谢模式存在显著差异，其中HOPO组和EVOO组分别有8、12个粪便生物标志物和15、6个血清生物标志物，同时，粪便和血清中氨基酸、多肽及其类似物组成发生显著变化，而支链氨基酸(BCAAs)生物合成通路被鉴定为HOPO和EVOO的主要调控途径，是高油酸花生油和特级初榨橄榄油预防大鼠膳食诱导代谢综合征的关键通路。
Abstract High-oleic acid peanut oil (HOPO) and extra-virgin olive oil (EVOO) have been reported previously to have an attenuating effect on metabolic syndrome (MS). This study aimed to evaluate the metabolic effect of HOPO and EVOO supplementation in attenuating MS and the role of gut microbiota in regulating the metabolic profile. Sprague-Dawley rats were continuously fed with a normal diet, high-fructose and high-fat (HFHF) diet, HFHF diet containing HOPO, or a HFHF diet containing EVOO for 12 weeks. The metabolomics profiles of feces and serum samples were compared using untargeted metabolomics based on UPLC-Q/TOF-MS. Partial Least Squares Discriminant Analysis (PLS-DA) was used to identify the potential fecal and serum biomarkers from different groups. Correlation between gut microbiota and biomarkers was assessed, and pathway analysis of serum biomarkers was conducted. Differences in metabolic patterns in feces and serum were observed among different groups. There were 8 and 12 potential biomarkers in feces and 15 and 6 potential biomarkers in serum of HOPO group and EVOO group, respectively, suggesting that HOPO and EVOO supplementation mainly altered amino acids, peptides, and their analogs in feces and serum. The branched-chain amino acids (BCAAs) biosynthesis pathway was identified as a major pathway regulated by HOPO or EVOO. This study suggests that HOPO and EVOO supplementation ameliorate diet-induced MS, mainly via modulation of the BCAAs biosynthesis pathway.
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Received: 09 February 2021
Accepted: 09 October 2021
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| Fund: This research was supported by the Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (CAAS-ASTIP-201XIAPPST), the Top Young Talents of Grain Industry in China (LQ2020202), and the National Natural Science Foundation of China (32172149). |
| About author: Correspondence WANG Qiang, E-mail: wangqiang06@caas.cn |
Cite this article:
ZHAO Zhi-hao, SHI Ai-min, GUO Rui, LIU Hong-zhi, HU Hui, WANG Qiang.
2022.
Protective effect of high-oleic acid peanut oil and extra-virgin olive oil in rats with diet-induced metabolic syndrome by regulating branched-chain amino acids metabolism. Journal of Integrative Agriculture, 21(3): 878-891.
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