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Journal of Integrative Agriculture  2022, Vol. 21 Issue (3): 878-891    DOI: 10.1016/S2095-3119(21)63851-0
Special Issue: 食品科学合辑Food Science
Food Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Protective effect of high-oleic acid peanut oil and extra-virgin olive oil in rats with diet-induced metabolic syndrome by regulating branched-chain amino acids metabolism
ZHAO Zhi-hao1, SHI Ai-min1, 2, GUO Rui1, LIU Hong-zhi1, 2, HU Hui1, WANG Qiang1, 2
1 Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, P.R.China
2 Collaborative Innovation Center for Modern Grain Circulation and Safety, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, P.R.China
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Abstract  High-oleic acid peanut oil (HOPO) and extra-virgin olive oil (EVOO) have been reported previously to have an attenuating effect on metabolic syndrome (MS).  This study aimed to evaluate the metabolic effect of HOPO and EVOO supplementation in attenuating MS and the role of gut microbiota in regulating the metabolic profile.  Sprague-Dawley rats were continuously fed with a normal diet, high-fructose and high-fat (HFHF) diet, HFHF diet containing HOPO, or a HFHF diet containing EVOO for 12 weeks.  The metabolomics profiles of feces and serum samples were compared using untargeted metabolomics based on UPLC-Q/TOF-MS.  Partial Least Squares Discriminant Analysis (PLS-DA) was used to identify the potential fecal and serum biomarkers from different groups.  Correlation between gut microbiota and biomarkers was assessed, and pathway analysis of serum biomarkers was conducted.  Differences in metabolic patterns in feces and serum were observed among different groups.  There were 8 and 12 potential biomarkers in feces and 15 and 6 potential biomarkers in serum of HOPO group and EVOO group, respectively, suggesting that HOPO and EVOO supplementation mainly altered amino acids, peptides, and their analogs in feces and serum.  The branched-chain amino acids (BCAAs) biosynthesis pathway was identified as a major pathway regulated by HOPO or EVOO.  This study suggests that HOPO and EVOO supplementation ameliorate diet-induced MS, mainly via modulation of the BCAAs biosynthesis pathway.
Keywords:  metabolic syndrome        high-oleic acid peanut oil       extra-virgin olive oil       metabolomics       UPLC-Q/TOF-MS  
Received: 09 February 2021   Accepted: 09 October 2021
Fund: This research was supported by the Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (CAAS-ASTIP-201XIAPPST), the Top Young Talents of Grain Industry in China (LQ2020202), and the National Natural Science Foundation of China (32172149).
About author:  Correspondence WANG Qiang, E-mail:

Cite this article: 

ZHAO Zhi-hao, SHI Ai-min, GUO Rui, LIU Hong-zhi, HU Hui, WANG Qiang. 2022. Protective effect of high-oleic acid peanut oil and extra-virgin olive oil in rats with diet-induced metabolic syndrome by regulating branched-chain amino acids metabolism. Journal of Integrative Agriculture, 21(3): 878-891.

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