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| Exogenous Application of Abscisic Acid or Gibberellin Acid Has Different Effects on Starch Granule Size Distribution in Grains of Wheat |
| PENG Dian-liang, CAI Tie, YIN Yan-ping, YANG Wei-bing, NI Ying-li, YANG Dong-qing , WANG |
| National Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China |
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摘要 Granule size distribution of wheat starch is an important characteristic that can affect its chemical composition and the functionality of wheat products. Two high-yield winter wheat cultivars were used to evaluate the effects of the application of exogenous ABA or GA during the reproductive phase of the initial grain filling on starch granule size distribution and starch components in grains at maturity. The results indicated that a bimodal curve was found in the volume and surface area distribution of grain starch granules, and a unimodal curve was observed for the number distribution under all treatments. The exogenous ABA resulted in a significant increase in the proportions (both by volume and by surface area) of B-type (<9.9 μm in diameter) starch granules, with a reduction in those of A-type (>9.9 μm) starch granules, while, the exogenous GA3 led to converse effects on size distribution of those starch granules. The exogenous ABA also increased starch, amylose and amylopectin contents at maturity but significantly reduced the ratio of amylose to amylopectin. Application of GA3 significantly reduced starch content, amylopectin content but increased the ratio of amylose to amylopectin. The ratio of amylose to amylopectin showed a significant and negative relationship with the volume proportion of granules <9.9 μm, but was positively related to the volume proportion of granules 22.8-42.8 μm.
Abstract Granule size distribution of wheat starch is an important characteristic that can affect its chemical composition and the functionality of wheat products. Two high-yield winter wheat cultivars were used to evaluate the effects of the application of exogenous ABA or GA during the reproductive phase of the initial grain filling on starch granule size distribution and starch components in grains at maturity. The results indicated that a bimodal curve was found in the volume and surface area distribution of grain starch granules, and a unimodal curve was observed for the number distribution under all treatments. The exogenous ABA resulted in a significant increase in the proportions (both by volume and by surface area) of B-type (<9.9 μm in diameter) starch granules, with a reduction in those of A-type (>9.9 μm) starch granules, while, the exogenous GA3 led to converse effects on size distribution of those starch granules. The exogenous ABA also increased starch, amylose and amylopectin contents at maturity but significantly reduced the ratio of amylose to amylopectin. Application of GA3 significantly reduced starch content, amylopectin content but increased the ratio of amylose to amylopectin. The ratio of amylose to amylopectin showed a significant and negative relationship with the volume proportion of granules <9.9 μm, but was positively related to the volume proportion of granules 22.8-42.8 μm.
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Received: 07 September 2012
Accepted:
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| Fund: This research was supported by the National Natural Science Foundation of China (31271661, 30871477), the National Basic Program of China (2009CB118602), and the Special Fund for Agro-Scientific Research in the Public Interest of China (201203100). |
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Corresponding Authors:
Correspondence WANG Zhen-lin, Tel: +86-538-8241359, E-mail: zlwang@sdau.edu.cn
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Cite this article:
PENG Dian-liang, CAI Tie, YIN Yan-ping, YANG Wei-bing, NI Ying-li, YANG Dong-qing , WANG .
2013.
Exogenous Application of Abscisic Acid or Gibberellin Acid Has Different Effects on Starch Granule Size Distribution in Grains of Wheat. Journal of Integrative Agriculture, 12(9): 1551-1559.
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| [1]Ahmadi A, Baker D A. 1999. Effects of abscisic acid (ABA)on grain filling processes in wheat. Plant GrowthRegulation, 28, 187-197[2]Bechtel D B, Zayas I, Kaleikau L, Pomeranz Y. 1990. Sizedistributionof wheat starch granules during endospermdevelopment. Cereal Chemistry, 67, 59-63[3]Chiotelli E, Meste M L. 2002. Effect of small and the largewheat starch granules on thermo mechanical behaviorof starch. Cereal Chemistry, 79, 286-293[4]Dai Z M, Yin Y P, Zhang M, Li W Y, Yan S H, Cai R G, WangZ L. 2008. Starch granule size distribution in wheat grainsunder irrigated and rainfed conditions. ActaAgronomica Sinica, 34, 795-802[5]Ellis R P, Cochrane M P, Dale M F B, Duffus C M, Lynn A,Morrison I M, Prentice R D M, Swanston J S, Tiller S A.1998. Starch production and industrial use. Journal ofthe Science of Food and Agriculture, 77, 289-311[6]He Z F. 1985. Analysis Technique for Grain Quality inCereals and Oils. China Agriculture Press, Beijing. pp.230-257[7](in Chinese)Hurkman W J, McCue K F, Altenbach S B. 2003. Effect oftemperature on expression of genes encoding enzymesfor starch biosynthesis in developing wheat endosperm. Plant Science, 164, 873-881[8]Kim H S, Huber K C. 2008. Channels within soft wheatstarch A- and B-type granules. Journal of CerealScience, 48, 159-172[9]Li W Y, Yin Y P, Yan S H, Wang Z L. 2010. Starch granulesize distribution in wheat grain in relation to shadingafter anthesis. The Journal of Agricultural Science,148, 183-189[10]Lindeboom N, Chang P R, Tyler R T. 2004. Analytical,biochemical and physicochemical aspects of starchgranule size, with emphasis on small granule starches:a review. Starch-Starke, 56, 89-99[11]Liu Y E, Liu P. 2010. Hormonal changees caused by theXenia effect during grain filling of normal corn and highoilcorncrosses. Crop Science, 50, 215-221[12]MacLeod L C, Duffus C M. 1988. Temperature effects onstarch granules in developing barley grains. Journalof Cereal Science, 8, 29-37[13]Malouf R B, Hoseney R C. 1992. Wheat hardness. I. A methodto measure endosperm tensile strength using tabletsmade from wheat flour. Cereal Chemistry, 69, 164-168[14]Ni Y, Wang Z, Yin Y, Li W, Yan S, Cai T. 2011. Starchgranule size distribution in wheat grain in relation tophosphorus fertilization. The Journal of AgriculturalScience, 10, 1-8[15]Park S H, Chung O K, Seib P A. 2004. Size distribution andproperties of wheat starch granules in relation to crumbgrain score of pup-loaf bread. Cereal Chemistry, 81,699-704[16]Park S H, Wilson J D, Seabourn B W. 2009. Starch granulesize distribution of hard red winter and hard red springwheat: its effects on mixing and bread making quality.Journal of Cereal Science, 49, 98-105[17]Peng M S, Gao M. 1999. Separation and characterization ofA- and B-type starch granules in wheat endosperm.Cereal Chemistry, 76, 375-379[18]Peterson D G, Fulcher R G. 2001. Variation in MinnesotaHRS wheats: starch granule size distribution. FoodResearch International, 34, 357-363[19]Sahlstrom S, Baevre A B, Brathen E. 2003. Impact of starchproperties on hearth bread characteristics. II. PurifiedA- and B-granule fractions. Journal of Cereal Science,37, 285-293[20]Singh S, Singh G, Singh P, Singh N. 2008. Effect of waterstress at different stages of grain development on thecharacteristics of starch and protein of different wheatcultivars. Food Chemistry, 108, 130-139[21]Soh H N, Sissons M J, Turner M A. 2006. Effect of starchgranule size distribution and elevated amylose contenton durum dough rheology and spaghetti cookingquality. Cereal Chemistry, 83, 513-519[22]Travaglia C, Cohen A C, Reinoso H, Castillo C, Bottini R.2007. Exogenous abscisic acid increases carbohydrateaccumulation and redistribution to the grains in wheatgrown under field conditions of soil water restriction.Plant Growth Regulation, 26, 285-289[23]Vermeylen R, Goderis B, Reynaers H, Delcour J A. 2005.Gelatinisation related structural aspects of small and largewheat starch granules. Carbohydr Polymers, 62, 170-181[24]Wang C Y, Ma D Y, Zhu Y J, Guo T C, Feng W, Zhou S M.2004. Effects of different irrigation and nitrogenapplication regimes in winter wheat on cooking qualitiesof Chinese noodle. Scientia Agricultura Sinca, 7, 256-262 (in Chinese)[25]Wilson J D, Bechtel D B, Todd T C, Seib P A. 2006.Measurement of wheat Starch granule size distributionusing image analysis and laser diffraction technology.Cereal Chemistry, 83, 259-268[26]Xie Z J, Jiang D, Cao W X, Dong T B, Jing Q. 2003.Relationships of endogenous plant hormones toaccumulation of grain protein and starch in winter wheatunder different post-anthesis soil water statuses. PlantGrowth Regulation, 41, 117-127[27]Xu G W, Zhang J H, Lam H M, Wang Z Q, Yang J C. 2007.Hormonal changes are related to the poor grain fillingin the inferior spikelets of rice cultivated under nonfloodedand mulched condition. Field Crops Research,101, 53-61[28]Yang J C, Wang Z Q, Zhu Q S, Su B L. 1999. Regulation ofABA or GA to grain filling of rice. Acta AgronomicaSinica, 3, 343-348[29]Yang J C, Zhang J H. 2006. Grain filling of cereals under soildrying. New Phytologis, 169, 223-236[30]Yang J C, Zhang J H, Wang Z Q, Zhu Q S, Wang W. 2001.Hormonal changes in the grains of rice subjected towater stress during grain filling. Plant Physiology, 127,315-323[31]Yang W B, Wang Z L, Yin Y P, Li W Y, Li Y, Chen X G,Wang P, Chen E Y, Guo J X, Cai T, et al. 2011. Effects ofspraying exogenous ABA or GA on the endogenoushormones concentration and filling of wheat grains.Scientia Agricultura Sinca, 44, 2673-2682[32](in Chinese)Yu S L. 1990. Wheat in Shandong Province. ChinaAgriculture Press, Beijing. (in Chinese)Zeng M, Morris C F, Batey I L, Wrigley C W. 1997. Sources ofvariation for starch gelatinization, pasting, and gelationproperties of wheat. Cereal Chemistry, 74, 63-71[33]Zhang H, Tan G, Yang L, Yang J, Zhang J, Zhao B. 2009.Hormones in the grains and roots in relation topostanthesis development of inferior and superiorspikelets in japonica/indica hybrid rice. PlantPhysiology and Biochemistry, 47, 195-204[34]Zhang T, Wang Z, Yin Y, Cai R, Yan S, Li W. 2010. Starchcontent and granule size distribution in grains of wheatin relation to post-anthesis water deficits. Journal ofAgronomy and Crop Science, 196, 1-8[35]Zhao H, Dai T, Jiang D, Cao W. 2008. Effects of hightemperature on key enzymes involved in starch andprotein formation in grains of two wheat cultivars.Journal of Agronomy and Crop Science, 194, 47-54 |
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