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Journal of Integrative Agriculture  2025, Vol. 24 Issue (10): 3703-3718    DOI: 10.1016/j.jia.2025.02.010
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Abscisic acid reduces Cd accumulation by regulating Cd transport and cell wall sequestration in rice

Zhijun Xu1, 2*, Jiashi Peng3*, Yanlei Fu4*, Jing Zhao2, 5, Yan Peng1, Bohan Liu1, Xujun Hu4, Yuchuan Liu4, Meijuan Duan1, 6, Nenghui Ye1#, Zhenxie Yi1#, Shuan Meng1, 2#

1 Hunan Provincial Key Laboratory of Rice Stress Biology/College of Agronomy, Hunan Agricultural University, Changsha 410128, China

2 Yuelushan Laboratory, Changsha 410128, China

3 Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, Hunan University of Science and Technology, Xiangtan 411201, China

4 OE Biotech Co., Ltd., Shanghai 201112, China

5 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China

6 Hunan Women’s University, Changsha 410004, China 

 Highlights 

Numerous abscisic acid (ABA)-related genes are responsive to cadmium (Cd) stress in rice plants.
ABA reduces Cd accumulation by suppressing Cd uptake and regulating cell wall sequestration.
Foliar spraying of ABA during the grain-filling stage reduces Cd accumulation in rice grains.

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摘要  

镉影响水稻生长其随食物链迁移进入人体严重威胁人类的健康,因此,研究水稻对镉的响应和调控过程显得尤为重要。本研究通过转录组分析发现大量脱落酸相关的基因响应镉胁迫,后续通过施加外源脱落酸可以显著降低水稻地上部和根部的镉含量。不仅如此外源脱落酸可能通过提高抗坏血酸过氧化物酶活性以及降低过氧化氢水平缓解镉对水稻的毒害。后续研究表明,脱落酸可以通过调水稻对的转运和细胞壁的阻隔作用,减少水稻植株的镉积累。进一步的,脱落酸信号因子OsABI5突变可导致水稻地上部镉含量提高。此外,在水稻灌浆期叶面喷施脱落酸显著降低水稻籽粒的镉积累,并主要通过降低水稻对镉的吸收抑制镉从根部向地上部转运以及抑制镉从叶片向籽粒的转运实现。本研究不仅揭示了脱落酸在调节水稻镉胁迫响应的潜在机制,为应对农田镉污染提供了参考。



Abstract  

Cadmium (Cd) uptake by rice plants and its subsequent movement through food chains pose a notable risk to the health of both plants and humans.  Therefore, understanding the fundamental mechanisms underlying the uptake and movement process is essential.  Through transcriptome analysis, we found that numerous abscisic acid (ABA)-related genes responded to Cd stress.  Exogenous application of ABA significantly reduced Cd accumulation in the shoots and roots of rice plants.  The increased ascorbate peroxidase (APX) enzyme activity, decreased H2O2 content, and elevated Cd tolerance index collectively suggest that ABA may mitigate the toxicity of Cd in rice plants.  Further study revealed that exogenous ABA reduced Cd accumulation by regulating Cd transport and cell wall sequestration.  Consistently, mutation of the ABA signaling factor OsABI5 resulted in a significant increase in Cd accumulation in shoots.  Moreover, foliar spraying of ABA during the grain-filling stage significantly reduced Cd accumulation in rice grains, which was attributed mainly to decreased Cd uptake and the inhibition of Cd transportation from roots to shoots and from leaves to grains.  These findings elucidate the underlying mechanisms of the ABA-mediated response to Cd stress in rice and provide a practical reference for coping with Cd pollution in farmlands

Keywords:  rice       Cd accumulation        Cd toxicity        abscisic acid        cell wall  
Received: 26 November 2024   Accepted: 26 December 2024 Online: 10 February 2025  
Fund: 

The authors acknowledge support from the National Natural Science Foundation of China (U20A2024) and the National Key Research and Development Program of China (2023YFD2301300).

About author:  Zhijun Xu, E-mail: 1136035895@qq.com; #Correspondence Shuan Meng, E-mail: mengs@hunau.edu.cn; Zhenxie Yi, E-mail: yizhenxie@126.com; Nenghui Ye, E-mail: laonengye@gmail.com * These authors contributed equally to this study.

Cite this article: 

Zhijun Xu, Jiashi Peng, Yanlei Fu, Jing Zhao, Yan Peng, Bohan Liu, Xujun Hu, Yuchuan Liu, Meijuan Duan, Nenghui Ye, Zhenxie Yi, Shuan Meng. 2025. Abscisic acid reduces Cd accumulation by regulating Cd transport and cell wall sequestration in rice. Journal of Integrative Agriculture, 24(10): 3703-3718.

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