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| Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae |
| WANG Ying, JIAO Tian-lei, LIU Xiao-hong, LIN Fu-cheng , WU Wei-ren |
1. Department of Agronomy, College of Agriculture & Biotechnology
2. Institute of Biotechnology, College of Agriculture & Biotechnology
3. School of Life Sciences, Fujian Agriculture & Forestry University |
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摘要 Magnaporthe oryzae, a filamentous ascomycete fungus, is well known as the causal agent of rice blast. With thetechnology of suppression subtractive hybridization (SSH), it was previously found that MGG_06001 (or named MoNEM1),a gene of M. oryzae homologous to the NEM1 (nuclear envelope morphology protein 1) gene of baker’s yeast(Saccharomyces cerevisiae), is differentially expressed between the mature appressium and the conidium and mycelium.This study aimed to characterize the function of MoNEM1 gene by knocking it out using the method of target genereplacement. The ΔMonem1 mutants exhibited reduced mycelial growth and conidiation. However, disruption of MoNEM1gene does not affect the pathogenicity of M. oryzae on barley and rice.
Abstract Magnaporthe oryzae, a filamentous ascomycete fungus, is well known as the causal agent of rice blast. With thetechnology of suppression subtractive hybridization (SSH), it was previously found that MGG_06001 (or named MoNEM1),a gene of M. oryzae homologous to the NEM1 (nuclear envelope morphology protein 1) gene of baker’s yeast(Saccharomyces cerevisiae), is differentially expressed between the mature appressium and the conidium and mycelium.This study aimed to characterize the function of MoNEM1 gene by knocking it out using the method of target genereplacement. The ΔMonem1 mutants exhibited reduced mycelial growth and conidiation. However, disruption of MoNEM1gene does not affect the pathogenicity of M. oryzae on barley and rice.
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Received: 08 December 2010
Accepted:
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| Fund: the National Natural Science Foundation of China (30671123 and 31000077). |
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Corresponding Authors:
Correspondence WU Wei-ren, Professor, Tel/Fax: +86-571-86971910, E-mail: wuwr@zju.edu.cn; LIN Fu-cheng, Professor,Tel/Fax: +86-571-86971185, E-mail: fuchenglin@zju.edu.cn
E-mail: wuwr@zju.edu.cn
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| About author: WANG Ying, Ph D, E-mail: wyhrx@126.com |
Cite this article:
WANG Ying, JIAO Tian-lei, LIU Xiao-hong, LIN Fu-cheng , WU Wei-ren.
2011.
Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae. Journal of Integrative Agriculture, 10(9): 1385-1390.
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| [1]Bonman J M, Vergel D D T, Khin M M. 1986. Physiologicspecialization of Pyricularia oryzae in the Philippines. PlantDisease, 70, 767-769.[2]Choi W, Dean R A. 1997. The adenylate cyclase gene MAC1 ofMagnaporthe grisea controls appressorium formation andother aspects of growth and development. The Plant Cell, 9,1973-1983.[3]Jeon J, Park S Y, Chi M H, Choi J, Park J, Rho H S, Kim S, GohJ, Yoo S, Choi J, et al. 2007. Genome-wide functional analysisof pathogenicity genes in the rice blast fungus. NatureGenetics, 39, 561-565.[4]Kadotani N, Nakayashiki H, Tosa Y, Mayama S. 2003. RNAsilencing in the phytopathogenic fungus Magnaporthe oryzae.Molecular Plant-Microbe Interactions, 16, 769-766.[5]Lau G W, Hamer J E. 1996. Regulatory genes controlling MPG1expression and pathogenicity in the rice blast fungusMagnaporthe grisea. The Plant Cell, 8, 771-781.[6]Li Y, Yan X, Wang H, Liang S, Ma W B, Fang M Y, Talbot N J,Wang Z Y. 2010. MoRic8 is a novel component of G-proteinsignaling during plant infection by the rice blast fungusMagnaporthe oryzae. Molecular Plant-Microbe Interactions,23, 317-331.[7]Liu T B, Chen G Q, Min H, Lin F C. 2009. MoFLP1, encoding anovel fungal fasciclin-like protein, is involved in conidiationand pathogenicity in Magnaporthe oryzae. Journal ofZhejiang University (Science B), 10, 434-444.[8]Liu S, Dean R A. 1997. G protein α-subunit genes control growth,development and pathogenicity of Magnaporthe grisea.Molecular Plant-Microbe Interactions, 10, 1075-1086.[9]Liu X H, Lu J P, Zhang L, Dong B, Min H, Lin F C. 2007.Involvement of a Magnaporthe grisea serine/threonine kinasegene, MgATG1, in appressorium turgor and pathogenesis.Eukaryotic Cell, 6, 997-1005.[10]Lu J P, Feng X X, Liu X H, Lu Q, Wang H K, Lin F C. 2007.Mnh6, a nonhistone protein, is required for fungaldevelopment and pathogenicity of Magnaporthe grisea.Fungal Genetics and Biology, 44, 819-829.[11]Oh Y, Donofrio N, Pan H Q, Coughlan S, Brown D E, Meng S,Mitchell T, Dean R A. 2008. Transcriptome analysis revealsnew insight into appressorium formation and function in therice blast fungus Magnaporthe oryzae. Genome Biology, 9,R85. http://genomebiology.com/content/9/5/R85Siniossoglou S, Santos-Rosa H, Rappsilber J, Mann M, Hurt E.1998. A novel complex of membrane proteins required forformation of a spherical nucleus. The EMBO Journal, 17,6449-6464.[12]Son S, Osmani S A. 2009. Analysis of all protein phosphatasegenes in Aspergillus nidulans identifies a new mitoticregulator, Fcp1. Eukaryotic Cell, 8, 573-585.[13]Talbot N J, Ebbole D J, Hamer J E. 1993. Identification andcharacterization of MPG1, a gene involved in pathogenicityfrom the rice blast fungus Magnaporthe grisea. The PlantCell, 5, 1575-1590.[14]Talbot N J, Kershaw M J, Wakley G E, de Vries O, Wessels J,Hamer J E. 1996. MPG1 encodes a fungal hydrophobininvolved in surface interactions during infection-relateddevelopment of Magnaporthe grisea. The Plant Cell, 8, 985-999.[15]Viaud M C, Balhadere P V, Talbot N J. 2002. A Magnaporthegrisea cyclophilin acts as a virulence determinant during plantinfection. The Plant Cell, 14, 917-930.[16]Xu J R, Hamer J E. 1996. MAP kinase and cAMP signalingregulate infection structure formation and pathogenic growthin the rice blast fungus Magnaporthe grisea. Genes &Development, 10, 2696-2706.[17]Xue C Y, Park G, Choi W, Zheng L, Dean R A, Xu J R. 2002.Two novel fungal virulence genes specifically expressed inappressoria of the rice blast fungus. The Plant Cell, 14, 2107-2119.[18]Yang J, Zhao X Y, Sun J, Kang Z S, Ding S L, Xu J R, Peng Y L.2010. A novel protein Com1 is required for normal conidiummorphology and full virulence in Magnaporthe oryzae.Molecular Plant-Microbe Interactions, 23, 112-123. |
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