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| Identification and Expression of a β-actin Gene from Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae) |
| JIANG Hong-bo1, SHEN Guang-mao1, DOU Wei1, TANG Pei-an1, 2, LIU Yong-hua1, ZHOU An-wei1, WANG Jin-jun1 |
1. Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University
2. College of Food Science and Engineering, Nanjing University of Finance and Economics |
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摘要 A β-actin gene, Libβ-actin1, from the psocid, Liposcelis bostrychophila, was isolated, sequenced, and expressed inEscherichia coli. The cDNA sequence was 1 281 bp in length and contained an open reading frame of 1 131 bp encoding376 amino acids with a predicted molecular weight of 41.82 kDa. According to a BlastN search, the coding region sharedthe highest identity (97%) with Pediculus humanus actin 5C, while the deduced amino acid sequence was completelyidentical to a mutant of Drosophila melanogaster actin 5C. Comparison of the nucleotide and deduced amino acidsequences confirmed the high similarity between Libβ-actin1 and homologs in other insect species. The 3´ untranslatedregion (3´ UTR) of the Libβ-actin1 mRNA had a high A+U content (approximately 75%) and contained three repeats of theAUUUUUA and AUUUA motifs, which may play a role in regulating mRNA decay. The expression of Libβ-actin1 wasfurther analyzed in insecticide induced and control psocids. The results indicated that there was no significant differencein expression of Libβ-actin1 between the induced and control groups, suggesting that Libβ-actin1 may be an appropriateinternal control for the gene expression profiling in this insect. Furthermore, Libβ-actin1 was also heterologouslyexpressed in Escherichia coli, which provided a basis to investigate the physiological functions of actin genes in thepsocid.
Abstract A β-actin gene, Libβ-actin1, from the psocid, Liposcelis bostrychophila, was isolated, sequenced, and expressed inEscherichia coli. The cDNA sequence was 1 281 bp in length and contained an open reading frame of 1 131 bp encoding376 amino acids with a predicted molecular weight of 41.82 kDa. According to a BlastN search, the coding region sharedthe highest identity (97%) with Pediculus humanus actin 5C, while the deduced amino acid sequence was completelyidentical to a mutant of Drosophila melanogaster actin 5C. Comparison of the nucleotide and deduced amino acidsequences confirmed the high similarity between Libβ-actin1 and homologs in other insect species. The 3´ untranslatedregion (3´ UTR) of the Libβ-actin1 mRNA had a high A+U content (approximately 75%) and contained three repeats of theAUUUUUA and AUUUA motifs, which may play a role in regulating mRNA decay. The expression of Libβ-actin1 wasfurther analyzed in insecticide induced and control psocids. The results indicated that there was no significant differencein expression of Libβ-actin1 between the induced and control groups, suggesting that Libβ-actin1 may be an appropriateinternal control for the gene expression profiling in this insect. Furthermore, Libβ-actin1 was also heterologouslyexpressed in Escherichia coli, which provided a basis to investigate the physiological functions of actin genes in thepsocid.
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Accepted:
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| Fund: This research was funded in part by the National NaturalSciences Foundation of China (30871631,31000860), the Specialized Research Fund for the DoctoralProgram of Higher Education, China(200806350009) and the Science and Technology InnovationFoundation for Graduate Students (kb2008001)of Southwest University, China. |
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Corresponding Authors:
Correspondence WANG Jin-jun, Professor, Tel: +86-23-68250255, Fax: +86-23-68251269,E-mail: jjwang7008@yahoo.com
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| About author: JIANG Hong-bo, Ph D, Tel: +86-23-68251642, E-mail: jhb8342@163.com |
Cite this article:
JIANG Hong-bo1, SHEN Guang-mao1, DOU Wei1, TANG Pei-an1, 2 , LIU Yong-hua1, ZHOU An-wei1, WANG Jin-jun1.
2011.
Identification and Expression of a β-actin Gene from Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae). Journal of Integrative Agriculture, 10(9): 1391-1401.
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| [1]Bunger M H, Langdahl B L, Andersen T, Husted L, Lind M,Eriksen E F, Bunger C E. 2003. Semiquantitative mRNAmeasurements of osteoinductive growth factors in humaniliac-crest bone: expression of LMP splice variants in humanbone. Calcified Tissue International, 73, 446-454.[2]Bustin S A. 2000. Absolute quantification of mRNA using realtimereverse transcription polymerase chain reaction assays.Journal of Molecular Endocrinology, 25, 169-193.[3]Cheng W X, Wang J J, Ding W, Zhao Z M. 2004. Inhibitionkinetics on carboxylesterase and acetylcholinesterase ofLiposcelis bostrychophila and Liposcelis entomophila(Psocoptera: Liposcelididae) of two insecticides. Journal ofApplied Entomology, 128, 292-297.[4]Dou W, Wang J J, Zhao Z M. 2006. Toxicological and biochemicalcharacterizations of GSTs in Liposcelis bostrychophilaBadonnel (Psocoptera: Liposcelidae). Journal of AppliedEntomology, 130, 251-256.[5]Drummond D R, Hennessey E S, Sparrow J C. 1991.Characterisation of missense mutations in the Act88F geneof Drosophila melanogaster. Molecular and GeneralGenetics, 226, 70-80.[6]Fyrberg E A, Mahaffey J W, Bond B J, Davidson N. 1983.Transcripts of the six Drosophila actin genes accumulate in astage- and tissue-specific manner. Cell, 33, 115-123.[7]Hirayoshi K, Kudo H, Takechi H, Nakai A, Iwamatsu A, YamadaK M, Nagata K. 1991. HSP47: a tissue-specific,transformation-sensitive, collagen-binding heat shock proteinof chicken embryo fibroblasts. Molecular and CellularBiology, 11, 4036-4044.[8]Horigane M, Ogihara K, Nakajima Y, Honda H, Taylor D. 2007.Identification and expression analysis of an actin gene fromthe soft tick, Ornithodoros moubata (Acari: Argasidae).Archives of Insect Biochemistry and Physiology, 64, 186-199.[9]Hwang U, Han M, Kim I, Lee Y, Aoki Y, Lee J. 2002. Cloningand sequences of ?-actin genes from Rhodeus notatus and thesilver carp Hypophthalmichthys molitrix (Cyprinidae) andthe phylogeny of cyprinid fishes inferred from ?-actin genes.DNA Sequence, 13, 153-159.[10]Jacinto A, Baum B. 2003. Actin in development. Mechanisms ofDevelopment, 120, 1337-1349.[11]Jiang H B, Liu Y H, Tang P A, Zhou A W, Wang J J. 2010.Validation of endogenous reference genes for insecticideinducedand developmental expression profiling of Liposcelisbostsrychophila (Psocoptera: Liposcelididae). MolecularBiology Reports, 37, 1019-1029.[12]Kabsch W, Vandekerckhove J. 1992. Structure and function ofactin. Annual Review of Biophysics and BiomolecularStructure, 21, 49-76.[13]Leong E C W, Ho S H. 1995. In vitro inhibition of esteraseactivity in Liposcelis bostrychophila Bad. and L. entomophila(Psocoptera: Liposcelididae). Comparative Biochemistry andPhysiology (Part B: Biochemistry and Molecular Biology),110, 121-130.[14]Mounier N, Gouy M, Mouchiroud D, Prudhomme J C. 1992.Insect muscle actins differ distinctly from invertebrate andvertebrate cytoplasmic actins. Journal of Molecular Evolution,34, 406-415.[15]Nayak M K, Collins P J, Kopittke R A. 2002. Comparativeresidual toxicities of carbaryl, deltamethrin and permethrinas structural treatments against three liposcelidid psocidspecies (Psocoptera: Liposcelididae) infesting storedcommodities. Journal of Stored Products Research, 38, 247-258.[16]Nayak M K, Collins P J, Kopittke R A. 2003. Residual toxicitiesand persistence of organophosphorus insecticides mixed withcarbaryl as structural treatments against three liposcelididpsocid species (Psocoptera: Liposcelididae) infesting storedgrain. Journal of Stored Products Research, 39, 343-353.[17]Nayak M K, Daglish G J. 2007. Combined treatments of spinosadand chlorpyrifos-methyl for management of resistant psocid pests (Psocoptera: Liposcelididae) of stored grain. PestManagement Science, 63, 104-109.[18]Nayak M K, Daglish G J, Byrne V S. 2005. Effectiveness ofspinosad as a grain protectant against resistant beetle andpsocid pests of stored grain in Australia. Journal of StoredProducts Research, 41, 455-467.[19]Otomo T, Tomchick D R, Otomo C, Panchal S C, Machius M,Rosen M K. 2005. Structural basis of actin filament nucleationand processive capping by a formin homology 2 domain.Nature, 433, 488-494.[20]Pollard T D. 2007. Regulation of actin filament assembly byArp2/3 complex and formins. Annual Review of Biophysicsand Biomolecular Structure, 36, 451-477.[21]Rayment I, Holden H, Whittaker M, Yohn C, Lorenz M, HolmesK, Milligan R. 1993. Structure of the actin-myosin complexand its implications for muscle contraction. Science, 261, 58-65.[22]Sambrook J, Russell D W. 2001. Molecular Cloning: A LaboratoryManual. 3rd ed. Cold Spring Harbor Laboratory Press, NewYork.Santos C R A, Power D M, Kille P, Llewellyn L, Ramsurn V,Wigham T, Sweeney G E. 1997. Cloning and sequencing of afull-length sea bream (Sparus aurata) ?-actin cDNA.Comparative Biochemistry and Physiology (Part B:Biochemistry and Molecular Biology), 117, 185-189.[23]da Silva Vaz J I, Imamura S, Nakajima C, Cardoso F C D, FerreiraC A S, Renard G, Masuda A, Ohashi K, Onuma M. 2005.Molecular cloning and sequence analysis of cDNAs encodingfor Boophilus microplus, Haemaphysalis longicornisand Rhipicephalus appendiculatus actins. VeterinaryParasitology, 127, 147-155.[24]Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: Molecularevolutionary genetics analysis (MEGA) software version 4.0.Molecular Biology and Evolution, 24, 1596-1599.[25]Tang T, Liu F S, Ren G D. 2008. Cloning, characterization andexpression detection of the ?-actin cDNA from Mantichorulasemenowi (Coleoptera: Tenebrionidae). Acta EntomologicaSinica, 51, 1210-1215. (in Chinese)[26]Thellin O, Zorzi W, Lakaye B, de Borman B, Coumans B, HennenG, Grisar T, Igout A, Heinen E. 1999. Housekeeping genesas internal standards: use and limits. Journal of Biotechnology,75, 291-295.[27]Tobin S L, Zulauf E, Sánchez F, Craig E A, McCarthy B J. 1980.Multiple actin-related sequences in the Drosophilamelanogaster genome. Cell, 19, 121-131.[28]van Troys M, Vandekerckhove J, Ampe C. 1999. Structuralmodules in actin-binding proteins: towards a newclassification. Biochimica et Biophysica Acta- MolecularCell Research, 1448, 323-348.[29]Turner B. 2005. Psocid infestations and domestic kitchens.Journal of Environmental Health Research, 5, 71-74.[30]Vandekerckhove J, Weber K. 1984. Chordate muscle actins differdistinctly from invertebrate muscle actins: The evolution ofthe different vertebrate muscle actins. Journal of MolecularBiology, 179, 391-413.[31]Wada A, Fukuda M, Mishima M, Nishida E. 1998. Nuclear exportof actin: a novel mechanism regulating the subcellularlocalization of a major cytoskeletal protein. The EMBOJournal, 17, 1635-1641.[32]Wang J J, Zhao Z M, Li L S. 1999. Induced tolerance of thepsocid, Liposcelis bostrychophila (Psocoptera:Liposcelididae) to controlled atmosphere. InternationalJournal of Pest Management, 45, 75-79. |
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