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Journal of Integrative Agriculture  2023, Vol. 22 Issue (6): 1833-1846    DOI: 10.1016/j.jia.2022.10.002
Animal Science · Veterinary Medicine Advanced Online Publication | Current Issue | Archive | Adv Search |
Effect of dioscorea opposite waste on growth performance, blood parameters, rumen fermentation and rumen bacterial community in weaned lambs
GUO Yun-xia1, 2, 3*, YANG Ruo-chen1*, DUAN Chun-hui1, WANG Yong1, HAO Qing-hong2, 3, JI Shou-kun1, YAN Hui1, ZHANG Ying-jie1#, LIU Yue-qin1#


1 College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, P.R.China

2 College of Life Sciences, Hebei Agricultural University, Baoding 071000, P.R.China

3 Engineering Research Center for Utilization of Agricultural Waste Resources, Baoding 071001, P.R.China

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摘要  

本研究选取60只健康状况良好、体重(22.68±2.56 kg)相近的雄性小尾寒羊断奶羔羊,采用完全随机区组试验设计,将60只羔羊随机分为4组,每组15只。DOW替代精料的比例分别为0% (对照组, CON)、 10%(DOW1)、 15%(DOW2)和20%(DOW3)。饲养期共63 d,预试期15 d,正试期48 d,进行羔羊生长性能、血液指标、瘤胃发酵参数和瘤胃微生物菌群的测定。结果表明,DOW2组羔羊的干物质(DMI)摄入量显著高于其他组(P<0.05)。日增重 (ADG) 随着DOW替代精料比例的增加而呈线性增加或二次效应变化(P<0.05),DOW2组羔羊的ADG较对照组更高(P<0.05)。随着DOW替代精料比例的增加,羔羊血浆GH、IGF-1和Insulin的浓度(P<0.05)呈现线性提高或二次效应变化(P<0.05), 且DOW2组血浆激素含量显著高于对照组。随着DOW替代精料比例的增加,血浆尿素氮(BUN)的浓度(P<0.05)呈线性降低和二次效应变化。DOW替代精料可有效的调节瘤胃代谢及菌群变化。随着DOW替代精料比例的增加,瘤胃NH3-N的浓度在饲喂后0 h、4 h和8 h呈线性降低(P<0.05),在0 h和4 h时呈二次效应变化(P<0.05);在饲喂后0 h和4 h后总挥发性脂肪酸(TVFAs)含量呈线性增加(P<0.05);在饲喂0 h乙酸的含量呈线性增加(P<0.05),饲喂后4 h和8 h时呈二次效应变化(P<0.05);丙酸的含量在饲喂0 h,4 h和8 h时呈线性降低,乙酸/丙酸比例在0 h,4 h和8 h均呈线性增加(P<0.05)。DOW2组瘤胃中厚壁菌门(Firmicutes)、琥珀酸菌属(Succiniclasticum)和反刍菌属(Ruminococcus_1)的相对丰度显著高于对照组,同时去铁杆菌门(Deferribacteres)、肠杆菌属(Intestinimonas)和反胃梭菌属(Ruminiclostridium)相对丰度显著低于对照组(P<0.05)。综上所述,麻山药下脚料可改善饲料适口性,增加DMI,进而调控瘤胃微生物菌群变化,提高瘤胃发酵效率,促进羔羊生长,在本试验条件下,综合生长性能、血液指标及瘤胃发酵参数,麻山药下脚料的适宜添加比例为15%。本试验首次对麻山药下脚料作为饲料原料对羔羊生长性能及瘤胃发酵参数进行了研究,为降低养殖成本,提高养殖效益,同时对农业废弃物的饲料化利用提供了思路。



Abstract  

This study investigated the effects of dioscorea opposite waste (DOW) on the growth performance, blood parameters, rumen fermentation and rumen microbiota of weaned lambs.  Sixty healthy weaned Small-Tailed Han lambs (male, (22.68±2.56) kg initially) were used as the experimental animals.  Four levels of concentrate: 0 (control, CON), 10% (DOW1), 15% (DOW2) and 20% (DOW3), were replaced with DOW in the basal diet as experimental treatments.  The results showed that lambs fed the DOW2 diet had a higher (P<0.05) dry matter intake (DMI) than the other groups.  There was no significant difference (P>0.05) among DOW groups in average daily weight gain (ADG), and replacing concentrate with DOW linearly or quadratically increased (P<0.05) the ADG, while lambs fed the DOW2 diet showed greater (P<0.05) ADG than the CON group.  The relative plasma concentration of growth hormone (GH), insulin like growth factor-1 (IGF-1) and insulin were affected by DOW, replacing concentrate with DOW linearly or quadratically (P<0.05) enhanced the plasma concentration of GH, IGF-1 and insulin, which was significantly higher (P<0.05) in the DOW2 group than in the CON, DOW1 and DOW3 groups.  In addition, the DOW treatment showed a lower (P<0.05) concentration of blood urea nitrogen (BUN) than the CON group.  Replacing concentrate with DOW quadratically decreased (P<0.05) the ruminal ammonia nitrogen (NH3-N) and increased (P<0.05) the total of volatile fatty acids (TVFAs) at 0 and 4 h after feeding as well as linearly decreased (P<0.05) the NH3-N at 8 h after feeding.  Replacing concentrate with DOW linearly decreased (P<0.05) the propionate and increased the aceate before feeding, and linearly decreased (P<0.05) propionate and quadratically increased (P<0.05) the aceate at 4 and 8 h after feeding.  Lambs fed the DOW2 diet increased the phylum Firmicutes and genera Succiniclasticum and Ruminococcus_1 groups, whereas decreased (P<0.05) the relative abundance of phylum Deferribacteres and genera intestinimonas and Ruminiclostridium.  In summary, replacing the concentrate with 15% DOW was beneficial for improving the rumen fermentation and ADG by increasing the DMI and modulating the rumen microbial community.

Keywords:  lamb       dioscorea opposite waste        growth performance        blood parameters        rumen fermentation        rumen microflora  
Received: 16 April 2022   Online: 04 October 2022   Accepted: 14 September 2022
Fund: This research was supported by the Key R&D Project of Hebei Province of China (21322907D and 21322910D), the Natural Science Foundation of Hebei Province, China (C2022204174), and the China Agriculture Research System (CARS-38 and CARS-39-23).
About author:  GUO Yun-xia, E-mail: gyx310@163.com; YANG Ruo-chen, E-mail: 1471981283@qq.com; #Correspondence ZHANG Ying-jie, Tel: +86-312-7528366, E-mail: zhangyingjie66@126.com; LIU Yue-qin, E-mail: liuyueqin66@126.com * These authors contributed equally to this study.

Cite this article: 

GUO Yun-xia, YANG Ruo-chen, DUAN Chun-hui, WANG Yong, HAO Qing-hong, JI Shou-kun, YAN Hui, ZHANG Ying-jie, LIU Yue-qin. 2023. Effect of dioscorea opposite waste on growth performance, blood parameters, rumen fermentation and rumen bacterial community in weaned lambs. Journal of Integrative Agriculture, 22(6): 1833-1846.

Afolabi T, Onadeji R, Ogunkunle O, Bamiro F. 2012. Comparative analysis of the nutritional quality of browse leaves (Spondias mombin and Albizia saman) and tuber peels (yam and cassava) used as ruminant feeds. IFE Journal of Science14, 337–344.

Aguerre M, Cajarville C, Perez-Ruchel A, Aguerre M, Pinacchio G, Repetto J L. 2011. Rumen pH and NH3-N concentration of sheep fed temperate pastures supplemented with sorghum grain. South African Journal of Animal Science39, 246–250.

Amat N, Amat R, Abdureyim S, Hoxur P, Osman Z, Mamut D, Kijjoa A. 2014. Aqueous extract of dioscorea opposita thunb normalizes the hypertension in 2K1C hypertensive rats. BMC Complementary and Alternative Medicine14, 36.

AOAC (Association of Official Analytical Chemists). 1990. Official methods of analysis: Changes in official methods of analysis made at the annual meeting. Oxford University Press, Great Clarendon Street, Oxford OX2 6DP, United Kingdom.

Bitsch R. 2003. Resources Council. Science and Technology Agency, Japan.

Broderick G A, Kang J H. 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science63, 64–75.

Caporaso J G, Kuczynski J, Stombaugh J, Bittinger K, Bushman F D, Costello E K, Fierer N, Pena A G, Goodrich J K, Gordon J I. 2010. QIIME allows analysis of high-throughput community sequencing data. Nature Methods7, 335–336.

Chen Y B, Lan D L, Tang C, Yang X N, Li J. 2015. Effect of DNA extraction methods on the apparent structure of yak rumen microbial communities as revealed by 16S rDNA sequencing. Polish Journal of Microbiology64, 29–36.

Chen Y F, Zhu Q, Wu S. 2015. Preparation of oligosaccharides from Chinese yam and their antioxidant activity. Food Chemistry173, 1107–1110.

Cheng G W. 2019. Study on the mechanism of dietary carbohydrate type regulating C2/C3 ratio in rumen fermentation. MSc thesis, Shandong Agricultural University, China. (in Chinese)

Colman E, Fokkink W B, Craninx M, Newbold J R, De Baets B, Fievez V. 2010. Effect of induction of subacute ruminal acidosis on milk fat profile and rumen parameters. Journal of Dairy Science93, 4759–4773.

Davidson S, Hopkins B, Diaz D, Bolt S, Brownie C, Fellner V, Whitlow L. 2003. Effects of amounts and degradability of dietary protein on lactation, nitrogen utilization, and excretion in early lactation Holstein cows. Journal of Dairy Science86, 1681–1689.

Do D T, Lam D H, Nguyen T, Mai T T P, Phan L T M, Vuong H T, Nguyen D V, Linh N T T, Hoang M N, Mai T P, Nguyen H H. 2021. Utilization of response surface methodology in optimization of polysaccharides extraction from vietnamese red ganoderma lucidum by ultrasound-assisted enzymatic method and examination of bioactivities of the extract. Scientific World Journal2021, 7594092.

Doko A S, Farougou S, Hountondji F. 2013. Impact of prophylactic measures and the use of local food resources on the viability and growth of pre-weaning lambs in Djougou, in the northern region of Benin. Journal of Animal and Plant Sciences19, 2933–2940.

Duthie C A, Haskell M, Hyslop J J, Waterhouse A, Wallace R J, Roehe R, Rooke J A. 2017. The impact of divergent breed types and diets on methane emissions, rumen characteristics and performance of finishing beef cattle. Animals11, 1762–1771.

Edgar R C. 2013. UPARSE: Highly accurate OTU sequences from microbial amplicon reads. Nature Methods10, 996–998.

Edgar R C, Haas B J, Clemente J C, Quince C, Knight R. 2011. UCHIME improves sensitivity and speed of chimera detection. Bioinformatics27, 2194–2200.

Eisemann J H, Tyrrell H F, Hammond A C, Reynolds P J, Bauman D E, Haaland G L, McMurtry J P, Varga G A. 1986. Effect of bovine growth hormone administration on metabolism of growing hereford heifers: Dietary digestibility, energy and nitrogen balance. Journal of Nutrition116, 157–163.

Ekenyem B, Madubuike F, Dike O. 2006. Effect of partial replacement of yam peel meal Dioscorea spp. for maize meal zea mays on performance and carcass characteristics of finisher broiler chicks. International Journal of Poultry Science5, 942–945.

Ekwe O, Nwali C, Mgbabu C, Nwonu S. 2020. Effect of supplementing maize with yam peel meal on the growth performance, carcass characteristics, and cost benefit of rabbit production. Adan Journal of Agriculture1, 131–138.

Epping J, Laibach N. 2020. An underutilized orphan tuber crop-Chinese yam: A review. Planta252, 1–19.

Etherton T D, Kensinger R S. 1984. Endocrine regulation of fetal and postnatal meat animal growth. Journal of Animal Science59, 511–528.

Garrity G M, Holt J M, Huber H, Stetter K O, Greene A C, Patel B K, Caccavo F, Allison M J, MacGregor B J, StahlD A. 2001. Phylum BIX. Deferribacteres phy. nov. In: Bergey’s Manual of Systematic Bacteriology. Springer, New York. pp. 465–471.

Graugnard D. Bionaz, M. Trevisi E, Moyes K, Salak-Johnson J, Wallace R, Drackley J, Bertoni G, Loor J. 2012. Blood immunometabolic indices and polymorphonuclear neutrophil function in peripartum dairy cows are altered by level of dietary energy prepartum. Journal of Dairy Science95, 1749–1758.

Ghimire S, Kohn R, Gregorini P, White R, Hanigan M. 2017. Representing interconversions among volatile fatty acids in the Molly cow model. Journal of Dairy Science100, 3658–3671.

Gutierrez C, Vera N, Allende R, Williams P, Avila-Stagno J. 2017. Effects of the inclusion of linseed and increasing concentrations of glycerol as replacement of corn grain on rumen fermentation, methane production and nutrient disappearance in a rusitec system. Journal of Animal Science95, 239.

Van Gylswyk N. 1995. Succiniclasticum ruminis gen. nov., sp. nov., a ruminal bacterium converting succinate to propionate as the sole energy-yielding mechanism. International Journal of Systematic and Evolutionary Microbiology45, 297–300.

Hua C, Tian J, Tian P, Cong R, Luo Y, Geng Y, Tao S, Ni Y, Zhao R. 2017. Feeding a high concentration diet induces unhealthy alterations in the composition and metabolism of ruminal microbiota and host response in a goat model. Frontiers in Microbiology8, 138.

Huang J, Li Y, Luo Y. 2017. Bacterial community in the rumen of Tibetan sheep and Gansu alpine fine-wool sheep grazing on the Qinghai–Tibetan Plateau, China. Journal of General and Applied Microbiology63, 122–130.

Hungate R E. 1975. The rumen microbial ecosystem. Annual Review of Ecology & Systematics6, 39–66.

Jami E, Israel A, Kotser A, Mizrahi I. 2013. Exploring the bovine rumen bacterial community from birth to adulthood. ISME Journal7, 1069–1079.

Jeon J R, Lee J S, Lee C H, Kim J Y, Kim S D, Nam D H. 2006. Effect of ethanol extract of dried Chinese yam (Dioscorea batatas) flour containing dioscin on gastrointestinal function in rat model. Archives of Pharmacal Research29, 348–353.

Ju Y, Xue Y, Huang J, Zhai Q, Wang X. 2014. Antioxidant Chinese yam polysaccharides and its pro-proliferative effect on endometrial epithelial cells. International Journal of Biological Macromolecules66, 81–85.

Khafipour E, Li S, Tun H, Derakhshani H, Moossavi S, Plaizier J. 2016. Effects of grain feeding on microbiota in the digestive tract of cattle. Animal Frontiers6, 13–19.

Kim S, Alam M, Gu M, Park K, Jeon C O, Ha J K, Cho K, Lee S. 2012. Effect of total mixed ration with fermented feed on ruminal in vitro fermentation, growth performance and blood characteristics of Hanwoo steers. Asian–Australasian Journal of Animal Sciences25, 213.

Kocherginskaya S A, Aminov R I, White B A. 2001. Analysis of the rumen bacterial diversity under two different diet conditions using denaturing gradient gel electrophoresis, random sequencing, and statistical ecology approaches. Anaerobe7, 119–134.

Koike S, Kobayashi Y. 2001. Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenesRuminococcus albus and Ruminococcus flavefaciensFEMS Microbiology Letters204, 361–366.

Kong X, Zhang Y, Wu X, Yin Y, Tan Z, Feng Y, Yan F, Bo M, Huang R, Li T. 2009. Fermentation characterization of Chinese yam polysaccharide and its effects on the gut microbiota of rats. International Journal of Microbiology2009, 1–13.

Kong X F, Zhang Y Z, Yin Y L, Wu G Y, Zhou H J, Tan Z L, Yang F, Bo M J, Huang R L, Li T J. 2009. Chinese Yam polysaccharide enhances growth performance and cellular immune response in weanling rats. Journal of the Science of Food and Agriculture89, 2039–2044.

Lee H R, Kong S Y, Sung S H, Kim H J. 2019. DA-9801 and its saponins, dioscin and protodioscin, protect primary cortical neurons from hyperglycemia-induced neurotoxicity. Journal of Functional Foods54, 231–240.

Li F, Zhang Z, Li X, Zhu B, Guo L, Li F, Weng X. 2020. Effect of duration of linseed diet supplementation before slaughter on the performances, meat fatty acid composition and rumen bacterial community of fattening lambs. Animal Feed Science and Technology263, 114457.

Li W J, Tao H, Zhang R, Zhang F, Xie B, Zhang N F, Diao Q Y. 2018. Effects of dietary fat levels on growth performance and serum parameters of early-weaned male twin Hu lambs. Acta Veterinaria et Zootechnica Sinica49, 327–337. (in Chinese)

Lin X Y, Wang J, Hou Q L, Wang Y, Hu Z Y, Shi K R, Yan Z G, Wang Z H. 2018. Effect of hay supplementation timing on rumen microbiota in suckling calves. Microbiology Open7, e00430.

Liu H J, Hu L Y, Han X P, Zhao N, Xu T W, Ma L, Wang X G, Zhang X L, Kang S P, Zhao X Q, Xu S X. 2020. Tibetan sheep adapt to plant phenology in alpine meadows by changing rumen microbial community structure and function. Frontiers in Microbiology11, 587558.

Liu Y, Wang C, Guo G, Huo W, Zhang Y, Pei C, Liu Q, Zhang S. 2019. Growth performance of post-weaned Holstein male calves accelerated by branched-chain volatile fatty acids addition with up-regulated hepatic mTOR expression via insulin and insulin-like growth factor-1 (IGF-1) signalling pathway. Journal of Animal and Feed Sciences28, 244–253.

Lund-Larsen T R, Sundby A, Kruse V, Velle W. 1977. Relation between growth rate, serum somatomedin and plasma testosterone in young bulls. Journal of Animal Science44, 189–194.

Luo D. 2008. Identification of structure and antioxidant activity of a fraction of polysaccharide purified from Dioscorea nipponica Makino. Carbohydrate Polymers71, 544–549.

Luo L, Zheng S, Huang Y, Qin T, Xing J, Niu Y, Bo R, Liu Z, Huang Y, Hu Y. 2016. Preparation and characterization of Chinese yam polysaccharide PLGA nanoparticles and their immunological activity. International Journal of Pharmaceutics511, 140–150.

Lyons T, Boland T, Storey S, Doyle E. 2017. Linseed oil supplementation of lambs’ diet in early life leads to persistent changes in rumen microbiome structure. Frontiers in Microbiology8, 1656.

Ma L, Xu S, Liu H, Xu T, Hu L, Zhao N, Han X, Zhang X. 2019. Yak rumen microbial diversity at different forage growth stages of an alpine meadow on the Qinghai–Tibet Plateau. Peer Journal7, e7645.

Marín-García P J, López-Luján M C, Ródenas L, Martínez-Paredes E M, Blas E, Pascual J J. 2020. Plasmatic urea nitrogen in growing rabbits with different combinations of dietary levels of lysine, sulphur amino acids and threonine. Animals10, 946.

Man W, Bing Y, Jun H, Jie Y, Luo Y H, Luo J Q, Mao X B, Chen D W. 2020. The toxicological effect of dietary excess of saccharicterpenin, the extract of camellia seed meal, in piglets. Journal of Integrative Agriculture19, 211–224.

Magoč T, Salzberg S L. 2011. FLASH: Fast length adjustment of short reads to improve genome assemblies. Bioinformatics27, 2957–2963.

McLoughlin S, Spillane C, Claffey N, Smith P E, O’Rourke T, Diskin M G, Waters S M. 2020. Rumen microbiome composition is altered in sheep divergent in feed efficiency. Frontiers in Microbiology11, 1981.

Meng X, Hu W, Wu S, Zhu Z, Lu R, Yang G, Qin C, Yang L, Nie G. 2019. Chinese yam peel enhances the immunity of the common carp (Cyprinus carpio L.) by improving the gut defence barrier and modulating the intestinal microflora. Fish & Shellfish Immunology95, 528–537.

Munnich M, Khiaosa-ard R, Klevenhusen F, Hilpold A, Khol-Parisini A, Zebeli Q. 2017. A meta-analysis of feeding sugar beet pulp in dairy cows: Effects on feed intake, ruminal fermentation, performance, and net food production. Animal Feed Science Technology224, 78–89.

Nakamura S, Haga S, Kimura K, Matsuyama S. 2018. Propionate and butyrate induce gene expression of monocarboxylate transporter 4 and cluster of differentiation 147 in cultured rumen epithelial cells derived from preweaning dairy calves. Journal of Animal Science96, 4902–4911.

NASEM (National Academies of Sciences Engineering Medicine). 2016. Nutrient Requirements of Beef Cattle. 8th ed. The National Academies Press, Washington.

Noziere P, Glasser F, Sauvant D. 2011. In vivo production and molar percentages of volatile fatty acids in the rumen: A quantitative review by an empirical approach. Animals5, 403–414.

Oliveira M A, Alves S P, Santos-Silva J, Bessa R J. 2016. Effects of clays used as oil adsorbents in lamb diets on fatty acid composition of abomasal digesta and meat. Animal Feed Science and Technology213, 64–73.

Owens F N, Basalan M. 2016. Ruminal Fermentation, Rumenology. Springer, Brazil. pp. 63–102.

Pruesse E, Quast C, Knittel K, Fuchs B M, Ludwig W, Peplies J, Glöckner F O. 2007. SILVA: A comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Research35, 7188–7196.

Saleem F, Bouatra S, Guo A C, Psychogios N, Mandal R, Dunn S M, Ametaj B N, Wishart D S. 2013. The bovine ruminal fluid metabolome. Metabolomics9, 360–378.

Salinas-Chavira J, Alvarez E, Montaño M, Zinn R. 2013. Influence of forage NDF level, source and pelletizing on growth performance, dietary energetics, and characteristics of digestive function for feedlot cattle. Animal Feed Science and Technology183, 106–115.

Shen Z M, Seyfert H M, Löhrke B, Schneider F, Zitnan R, Chudy A, Kuhla S, Hammon H M, Blum J W, Martens H, Hagemeister H, Voigt J. 2004. An energy-rich diet causes rumen papillae proliferation associated with more IGF type 1 receptors and increased plasma IGF-1 concentrations in young goats. Journal of Nutrition, 134, 11–17.

Van Soest P J, Robertson J B, Lewis B A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science74, 3583–3597.

Steel R G D, Torrie J H. 1980. Principles and Procedures of Statistics, a Biometrical Approach. McGraw-Hill Kogakusha, USA. p. 365.

Sun Z, Li J, Dai Y, Wang W, Shi R, Wang Z, Ding P, Lu Q, Jiang H, Pei W, Zhao X, Guo Y, Liu J, Tan X, Mao T. 2020. Indigo naturalis alleviates dextran sulfate sodium-induced colitis in rats via altering gut microbiota. Frontiers in Microbiology11, 731.

Tajima K, Arai S, Ogata K, Nagamine T, Matsui H, Nakamura M, Aminov R I, Benno Y. 2000. Rumen bacterial community transition during adaptation to high-grain diet. Anaerobe6, 273–284.

Tomczak D J, Samuelson K L, Jennings J S, Richeson J T. 2019. Oral hydration therapy with water and bovine respiratory disease incidence affects rumination behavior, rumen pH, and rumen temperature in high-risk, newly received beef calves. Journal of Animal Science97, 2015–2024.

Ukonu E, Ohanaka A, Uzegbu H, Onunkwo D, Oduehie T, Obih T, Ekenyem B. 2018. Response of finisher broiler birds fed graded levels of yam peel meal as an energy source in maize-based diet. Nigerian Journal of Animal Production45, 185–188.

Uyeno Y, Sekiguchi Y, Tajima K, Takenaka A, Kurihara M, Kamagata Y. 2010. An rRNA-based analysis for evaluating the effect of heat stress on the rumen microbial composition of Holstein heifers. Anaerobe16, 27–33.

Vos P, Garrity G, Jones D, Krieg N R, Ludwig W, Rainey F A, Schleifer K H, Whitman W B. 2011. Bergey’s Manual of Systematic Bacteriology. volume 3. The Firmicutes. Springer Science & Business Media, USA.

Wang L J, Zhang G N, Li Y, Zhang Y G. 2020. Effects of high forage/concentrate diet on volatile fatty acid production and the microorganisms involved in VFA production in cow rumen. Animals10, 223.

Wang Q, Garrity G M, Tiedje J M, Cole J R. 2007. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Applied and Environmental Microbiology73, 5261.

Xu C, Cai Y, Toyokawa K. 2009. Silage preparation and nutritive value of Chinese yam by-product. Animal Science Journal80, 398–402.

Xue D, Chen H, Zhao X, Xu S, Hu L, Xu T, Jiang L, Zhan W. 2017. Rumen prokaryotic communities of ruminants under different feeding paradigms on the Qinghai–Tibetan Plateau. Systematic and Applied Microbiology40, 227–236.

Yashim S, Haniel B, Makama S, Adama H. 2016. Growth performance, nutrient digestibility and nitrogen balance of red Sokoto bucks fed Irish potato (Solanum tuberosum L.) peels as a replacement for maize offal. Journal of Animal Production and Research28, 245–253.

Zhang J, Shi H, Wang Y, Cao Z, Yang H, Li S. 2018. Effect of limit-fed diets with different forage to concentrate ratios on fecal bacterial and archaeal community composition in Holstein heifers. Frontiers in Microbiology9, 976.

Zhang Z, Wang X, Liu C, Li J. 2016. The degradation, antioxidant and antimutagenic activity of the mucilage polysaccharide from Dioscorea oppositaCarbohydrate Polymers150, 227–231.

Zhao Y, Xie B, Gao J, Zhao G. 2020. Dietary supplementation with sodium sulfate improves rumen fermentation, fiber digestibility, and the plasma metabolome through modulation of rumen bacterial communities in steers. Applied Environmental Microbiology86, e01412–e01420.

Zheng W C, Hao X Y, Zhang H W, Xiang B W, Zhang W J, Zhang C X, Zhang J X. 2020, Effects of Saccharomyces cerevisiae and Bacillus licheniformis on growth performance and rumen fermentation in sheep. Scientia Agricultura Sinica, 53, 3385–3393. (in Chinese)

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