Please wait a minute...
Journal of Integrative Agriculture  2011, Vol. 10 Issue (7): 1049-1055    DOI: 10.1016/S1671-2927(11)60093-X
Original Articles Advanced Online Publication | Current Issue | Archive | Adv Search |
Occurrence of Glyphosate-Resistant Horseweed (Conyza canadensis) Population in China
Weed Research Laboratory, Nanjing Agricultural University
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  Horseweed (Conyza canadensis), an invasive alien weed, is one of the main weeds in orchards in China. Althoughglyphosate has been used for control of horseweed and many other weeds in orchards for more than 25 years in China, acase of glyphosate-resistant horseweed has not been identified in orchard in China so far despite glyphosate-resistanthorseweed cases have been reported in some other countries. Seeds of 25 horseweed populations were collected fromdifferent orchards with different glyphosate application history. Potted seedlings with 11-13-leaf growth stage weretreated with glyphosate at 0.035, 0.07, 0.14, 0.28, 0.56, 1.12, 2.24, 4.48, and 8.96 kg a.i. ha-1. The dosage dependenceresponse curve of each population was constructed with Log-logistic dose response regression equations. The ED50value of each population was calculated and compared with the susceptible population from China. Different populationshad different relative glyphosate-resistant levels which increased with the number of years of glyphosate application.Two populations with the highest resistance levels, 8.28 and 7.95 times, were found in Ningbo, Zhejiang Province, China,where glyphosate was used for weed control in orchards twice each year for 15 yr. The two resistant populationsaccumulated approximately two to four times less shikimic acid than the two susceptible populations 48 h after glyphosateapplication.

Abstract  Horseweed (Conyza canadensis), an invasive alien weed, is one of the main weeds in orchards in China. Althoughglyphosate has been used for control of horseweed and many other weeds in orchards for more than 25 years in China, acase of glyphosate-resistant horseweed has not been identified in orchard in China so far despite glyphosate-resistanthorseweed cases have been reported in some other countries. Seeds of 25 horseweed populations were collected fromdifferent orchards with different glyphosate application history. Potted seedlings with 11-13-leaf growth stage weretreated with glyphosate at 0.035, 0.07, 0.14, 0.28, 0.56, 1.12, 2.24, 4.48, and 8.96 kg a.i. ha-1. The dosage dependenceresponse curve of each population was constructed with Log-logistic dose response regression equations. The ED50value of each population was calculated and compared with the susceptible population from China. Different populationshad different relative glyphosate-resistant levels which increased with the number of years of glyphosate application.Two populations with the highest resistance levels, 8.28 and 7.95 times, were found in Ningbo, Zhejiang Province, China,where glyphosate was used for weed control in orchards twice each year for 15 yr. The two resistant populationsaccumulated approximately two to four times less shikimic acid than the two susceptible populations 48 h after glyphosateapplication.
Keywords:  horseweed [Conyza canadensis (L.) Cronq.]      glyphosate      resistance      shikimic acid  
Received: 27 July 2010   Accepted:
Corresponding Authors:  Correspondence QIANG Sheng, Professor, Tel: +86-25-84395117, E-mail: qiangs@njau.edu.cn, wrl@njau.edu.cn     E-mail:  sxl@njau.edu.cn
About author:  SONG Xiao-ling, Ph D, E-mail: sxl@njau.edu.cn;

Cite this article: 

SONG Xiao-ling, WU Jia-jun, ZHANG Hong-jun and QIANG Sheng. 2011. Occurrence of Glyphosate-Resistant Horseweed (Conyza canadensis) Population in China. Journal of Integrative Agriculture, 10(7): 1049-1055.

[1]       Atkinson D. 1985. Toxicological properties of glyphosate - asummary. In: Grossbord E, Atkinson D, eds., The HerbicideGlyphosate. Butterworth, Toronto. pp. 127-134, 210-216.
[2]       Baerson S R, Rodriguez D J, Biest D J, Tran M, You J S, KreugerR W, Dill G M, Pratley J E, Gruys K J. 2002. Investigatingthe mechanism of glyphosate resistance in rigid ryegrass(Lolium rigidum). Weed Science, 50, 721-730.
[3]       Baylis A. 2000. Why glyphosate is a global herbicide: strengths,weaknesses and prospects. Pest Management Science, 56,299-308.
[4]       Bradshaw L D, Padgette S R, Kimball S L, Wells B H. 1997.Perspectives on glyphosate resistance. Weed Technology, 11,189-198.
[5]       Caseley J, Copping L. 2000. Twenty-five years of increasingglyphosate use: the opportunities ahead. Pest ManagementScience, 56, 297.Cromartie T H, Polge N D. 2000. An improved assay for shikimicacid and its use as a monitor for the activity of sulfosate.Proceeding of Weed Science Society America, 40, 291.Culpepper A S, Grey T L, Vencill W K, Kichler J M, Webster TM, Brown S M, York A C, Davis J W, Hanna W W. 2006.Glyphosate-resistant Palmer amaranth (Amaranthus palmeri)confirmed in Georgia. Weed Science, 54, 620-626.
[6]       Feng P C C, Tran M, Chiu T, Sammons R D, Heck G R, CaJacobC A. 2004. Investigations into glyphosate-resistant horseweed(Conyza canadensis): retention, uptake, translocation, andmetabolism. Weed Science, 52, 498-505.
[7]       van Gessel M J. 2001. Glyphosate-resistant horseweed fromDelaware. Weed Science, 49, 703-705.
[8]       Heap I M. 2005. International survey of herbicide-resistantweeds. [2005-10-31].
[9]       http://www.weedscience.orgKoger C H, Reddy K N. 2005. Role of absorption andtranslocation in the mechanism of glyphosate resistance inhorseweed (Conyza canadensis). Weed Science, 53, 84-89.
[10]    Lee L J, Ngim J. 2000. A first report of glyphosate-resistantgoosegrass (Eleusine indica (L.) Gaertn) in Malaysia. PestManagement Science, 56, 336-339.
[11]    Lorraine-Colwill D F, Powles S B, Hawkes T R, Hollinshead PH, Warner S J, Preston C. 2002. Investigations into themechanism of glyphosate resistance in Lolium rigidum.Pesticide Biochemistry and Physiology, 74, 62-72.
[12]    Malik J, Barry G, Kishore G. 1989. The herbicide glyphosate.Biofactors, 2, 17-25.
[13]    Mueller T C, Massey J H, Hayes R M, Main C L, Stewart Jr CN. 2003. Shikimate accumulates in both glyphosate-sensitiveand glyphosate-resistant horseweed (Conyza canadensis L.Cronq.). Journal of Agricultural and Food Chemistry, 51,680-684.
[14]    Ng C H, Wickneswari R, Salmijah S, Teng Y T, Ismail B S. 2003.Gene polymorphisms in glyphosate-resistant and susceptiblebiotypes of Eleusine indica from Malaysia. Weed Research, 43, 108-115.
[15]    Perez A, Kogan M. 2003. Glyphosate-resistant Loliummultiflorum in Chilean orchards. Weed Research, 43, 12-19.
[16]    Perez-Jones A, Park K W, Colquhoun J, Mallory-Smith C, ShanerD. 2005. Identification of glyphosate-resistant Italian ryegrass(Lolium multiflorum) in Oregon. Weed Science, 53, 775-779.
[17]    Powles S, Preston C, Bryan I, Jutsum A. 1996. Herbicideresistance: Impact and management. Advances in Agronomy,58, 57-93.
[18]    Qiang S, Song X L, Wu J J. 2006. Resistant Pest Manage. 16(1),[2006-11-22].
[19]    http://www.weedscience.org/Case/Case.asp?ResistID=5277Sellers B A, Pollard J M, Smeda R J. 2005. Two common ragweed(Ambrosia artemisiifolia) biotypes differ in biology andresponse to glyphosate. Proceeding of Weed Science Society,45, 156.Simarmata M, Kaufmann J E, Penner D. 2003. Potential basis ofglyphosate resistance in California rigid ryegrass (Loliumrigidum). Weed Science, 51, 678-682.
[20]    Singh B K, Shaner D L. 1998. Rapid determination of glyphosateinjury to plants and identification of glyphosate-resistantplants. Weed Technology, 12, 527-530.
[21]    Steinrucken H, Amrhein N. 1980. The herbicide glyphosate is apotent inhibitor of 5-enolpyruvylshikimate-3-phosphatesynthase. Biochemical and Biophysical ResearchCommunications, 94, 1207-1212.
[22]    Tran M, Baerson S, Brinker R, Casagrande L, Faletti M, Feng Y,Nemeth M, Reynolds T, Rodriguez D, Shaffer D, et al. 1999.Characterization of glyphosate resistant Eleusine indicabiotypes from Malaysia. In: Proceedings of the 17th Asian-Pacific Weed Science Society Conference. pp. 527-536.
[23]    Urbano J M, Borrego A, Torres V, Jimenez C, Leon J M, BarnesJ. 2007. Glyphosate-resistant hairy fleabane (Conyzabonariensis) in Spain. Weed Technology, 21, 396-401.
[24]   Woodburn A. 2000. Glyphosate production, pricing and useworldwide. Pest Management Science, 56, 309-312.
[1] HUANG Hong-hao, LU Yi-xing, WU Su-juan, MA Zhen-bao, ZENG Dong-ping, ZENG Zhen-ling. Identification of blaIMI-mediated carbapenem-resistant Enterobacter from a duck farm in China[J]. >Journal of Integrative Agriculture, 2023, 22(8): 2500-2508.
[2] Tiago SILVA, Ying NIU, Tyler TOWLES, Sebe BROWN, Graham P. HEAD, Wade WALKER, Fangneng HUANG. Selection, effective dominance, and completeness of Cry1A.105/Cry2Ab2 dual-protein resistance in Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae)[J]. >Journal of Integrative Agriculture, 2023, 22(7): 2151-2161.
[3] LIU Yu, LIU Wen-wen, LI Li, Frederic FRANCIS, WANG Xi-feng. Transcriptome analysis reveals different response of resistant and susceptible rice varieties to rice stripe virus infection[J]. >Journal of Integrative Agriculture, 2023, 22(6): 1750-1762.
[4] ZHANG Yan, TIAN Tian, ZHANG Kun, ZHANG You-jun, WU Qing-jun, XIE Wen, GUO Zhao-jiang, WANG Shao-li.

Lack of fitness cost and inheritance of resistance to abamectin based on the establishment of a near-isogenic strain of Tetranychus urticae [J]. >Journal of Integrative Agriculture, 2023, 22(6): 1809-1819.

[5] WU Xian-xin, ZANG Chao-qun, ZHANG Ya-zhao, XU Yi-wei, WANG Shu, LI Tian-ya, GAO Li.

Characterization of wheat monogenic lines with known Sr genes and wheat cultivars for resistance to three new races of Puccinia graminis f. sp. tritici in China [J]. >Journal of Integrative Agriculture, 2023, 22(6): 1740-1749.

[6] Ambreen LEGHARI, Shakeel Ahmed LAKHO, Faiz Muhammad KHAND, Khaliq ur Rehman BHUTTO, Sameen Qayoom LONE, Muhammad Tahir ALEEM, Iqra BANO, Muhammad Ali CHANDIO, Jan Muhammad SHAH, LIN Hui-xing, FAN Hong-jie. Molecular epidemiology, characterization of virulence factors and antibiotic-resistance profile of Streptococcus agalactiae isolated from dairy farms in China and Pakistan[J]. >Journal of Integrative Agriculture, 2023, 22(5): 1514-1528.
[7] GAO Xian-xian, TANG Ya-ling, SHI Qing-yao, WEI Yu-shu, WANG Xiao-xue, SHAN Wei-xing, QIANG Xiao-yu. Vacuolar processing enzyme positively modulates plant resistance and cell death in response to Phytophthora parasitica infection[J]. >Journal of Integrative Agriculture, 2023, 22(5): 1424-1433.
[8] DONG Xiu-chun, QIAN Tai-feng, CHU Jin-peng, ZHANG Xiu, LIU Yun-jing, DAI Xing-long, HE Ming-rong. Late sowing enhances lodging resistance of wheat plants by improving the biosynthesis and accumulation of lignin and cellulose[J]. >Journal of Integrative Agriculture, 2023, 22(5): 1351-1365.
[9] LI Jiao-jiao, ZHAO Li, LÜ Bo-ya, FU Yu, ZHANG Shu-fa, LIU Shu-hui, YANG Qun-hui, WU Jun, LI Jia-chuang, CHEN Xin-hong. Development and characterization of a novel common wheat–Mexico Rye T1DL·1RS translocation line with stripe rust and powdery mildew resistance[J]. >Journal of Integrative Agriculture, 2023, 22(5): 1291-1307.
[10] SONG Zhong-ping, ZUO Yuan-yuan, XIANG Qin, LI Wen-jia, LI Jian, LIU Gang, DAI Shou-fen, YAN Ze-hong.

Investigation of Aegilops umbellulata for stripe rust resistance, heading date, and the contents of iron, zinc, and gluten protein [J]. >Journal of Integrative Agriculture, 2023, 22(4): 1258-1265.

[11] HU Wen-jing, FU Lu-ping, GAO De-rong, LI Dong-sheng, LIAO Sen, LU Cheng-bin. Marker-assisted selection to pyramid Fusarium head blight resistance loci Fhb1 and Fhb2 in a high-quality soft wheat cultivar Yangmai 15[J]. >Journal of Integrative Agriculture, 2023, 22(2): 360-370.
[12] Irshad AHMAD, Maksat BATYRBEK, Khushnuma IKRAM, Shakeel AHMAD, Muhammad KAMRAN, Misbah, Raham Sher KHAN, HOU Fu-jiang, HAN Qing-fang.

Nitrogen management improves lodging resistance and production in maize (Zea mays L.) at a high plant density [J]. >Journal of Integrative Agriculture, 2023, 22(2): 417-433.

[13] Jelli VENKATESH, Sung Jin KIM, Muhammad Irfan SIDDIQUE, Ju Hyeon KIM, Si Hyeock LEE, Byoung-Cheorl KANG. CopE and TLR6 RNAi-mediated tomato resistance to western flower thrips[J]. >Journal of Integrative Agriculture, 2023, 22(2): 471-480.
[14] SHAN Yan-fei, LI Meng-yan, WANG Run-ze, LI Xiao-gang, LIN Jing, LI Jia-ming, ZHAO Ke-jiao, WU Jun. Evaluation of the early defoliation trait and identification of resistance genes through a comprehensive transcriptome analysis in pears[J]. >Journal of Integrative Agriculture, 2023, 22(1): 120-138.
[15] Carlos Kwesi TETTEY, YAN Zhi-yong, MA Hua-yu, ZHAO Mei-sheng, GENG Chao, TIAN Yan-ping, LI Xiang-dong . Tomato mottle mosaic virus: characterization, resistance gene effectiveness, and quintuplex RT-PCR detection system[J]. >Journal of Integrative Agriculture, 2022, 21(9): 2641-2651.
No Suggested Reading articles found!