|
|
|
|
|
| Genetic Variation Among the Geographic Population of the Grain Aphid, Sitobion avenae (Hemiptera: Aphididae) in China Inferred from Mitochondrial COI Gene Sequence |
| College of Plant Protection, Shandong Agricultural University |
|
|
|
摘要 In order to characterize the genetic relationship of the geographic populations of Sitobion avenae (Hemiptera: Aphididae)in China, a 588 bp region of the mitochondrial cytochrome oxidase subunit I (mtDNA-COI) gene was sequenced andanalyzed among the different geographic populations. 269 individuals were collected from 17 localities in different wheatgrowingareas in China that covered most of the range reported for this species. Within the sequence among thesegeographic populations, 15 polymorphic sites defined 16 distinct haplotypes, ranging in sequence divergence from 0.2%(one nucleotide) to 1.7% (10 nucleotides). Of the 15 variable sites, 12 were transitional substitutions, 2 were transversionalsubstitutions and 1 was transitional and transversional substitution. Phylogenetic analysis showed that all haplotypeswere highly interconnected with each other, in absence of phylogeographic structing. Each of 8 haplotypes was foundonly at one locality, and the other haplotypes were the widespread distributed in the different localities. The highergenetic diversity was found in the northern China populations than that in the southern China populations. The lowgenetic differentiation (FST=-0.06945-0.69857) and high migration rate (Nm=0.21575-infinite) of Chinese populations suggestthat dispersal over long distance is a major factor in the demography of S. avenae.
Abstract In order to characterize the genetic relationship of the geographic populations of Sitobion avenae (Hemiptera: Aphididae)in China, a 588 bp region of the mitochondrial cytochrome oxidase subunit I (mtDNA-COI) gene was sequenced andanalyzed among the different geographic populations. 269 individuals were collected from 17 localities in different wheatgrowingareas in China that covered most of the range reported for this species. Within the sequence among thesegeographic populations, 15 polymorphic sites defined 16 distinct haplotypes, ranging in sequence divergence from 0.2%(one nucleotide) to 1.7% (10 nucleotides). Of the 15 variable sites, 12 were transitional substitutions, 2 were transversionalsubstitutions and 1 was transitional and transversional substitution. Phylogenetic analysis showed that all haplotypeswere highly interconnected with each other, in absence of phylogeographic structing. Each of 8 haplotypes was foundonly at one locality, and the other haplotypes were the widespread distributed in the different localities. The highergenetic diversity was found in the northern China populations than that in the southern China populations. The lowgenetic differentiation (FST=-0.06945-0.69857) and high migration rate (Nm=0.21575-infinite) of Chinese populations suggestthat dispersal over long distance is a major factor in the demography of S. avenae.
|
|
Accepted:
|
|
Corresponding Authors:
Correspondence CHEN Ju-lian, Professor, Tel: +86-10-62813685, Fax: +86-10-62895365, E-mail: cjl2000@hotmail.com
|
Cite this article:
XU Zhao-huan, CHEN Ju-lian, CHENG Deng-fa, LIU Yong and Frédéric Francis.
2011.
Genetic Variation Among the Geographic Population of the Grain Aphid, Sitobion avenae (Hemiptera: Aphididae) in China Inferred from Mitochondrial COI Gene Sequence. Journal of Integrative Agriculture, 10(7): 1041-1048.
|
| [1] Bae J; Kim I; Kim S; Jin B; Sohn H. 2001. Mitochondrial DNAsequence variation of the mushroom pest flies; Lycoriellamali (Diptera: Sciaridae) and Coboldia fuscipes (Diptera:Scatopsidae); in Korea. Applied Entomology and Zoology;36; 451-457.[2] Barrette R J; Crease T J; Hebert P D N; Via S. 1994. MitochondrialDNA diversity in the pea aphid Acyrthosiphon pissum.Genome; 37; 858-865.[3] Boulding E G. 1998. Molecular evidence against phylogeneticallydistinct host races of the pea aphid (Acyrthosiphon pisum).Genome; 41; 769-775.[4] Cai F H; Zhao H Y. 2004. Genetic polymorphism in naturalpopulations of Macrosiphum avenae. Journal of NorthwestA&F University (Natural Science Edition); 32; 21-24. (inChinese)[5] Cardenas L; Silva A X; Magoulas A; Cabezas J; Poulin E; OjedaF P. 2009. Genetic population structure in the Chilean jackmackerel; Trachurus murphyi (Nichols) across the SoutheasternPacific Ocean. Fisheries Research; 100; 109-115.[6] Clement M; Posada D; Crandall K A. 2000. TCS: a computerprogram to estimate gene genealogies. Molecular Ecology; 9;1657-1659.[7] Close R C; Tomlinsion A L. 1975. Dispersal of the grain aphidMacrosiphum miscanthi from Australia to New Zealand. TheNew Zealand Entomologist; 6; 62-65.[8] Dixon A F G. 1973. Biology of Aphids. Edward Arnold PublishersLtd.; London; UK.von Dohlen C D; Kurosu U; Aoki S. 2002. Phylogenetics andevolution of the eastern Asian-eastern North Americandisjunct aphid tribe; Hormaphidini (Hemiptera: Aphididae).Molecular Phylogenetics and Evolutionm; 23; 257-267.[9] Dong Q Z; Wei K; Meng Q X. 1987. Long distance migration ofSitobion miscanthi in Ningxia Province. Acta EntomologicaSinica; 30; 277-284. (in Chinese)[10] van Emden H F; Harrington R. 2007. Aphids as Crop Pests.CABI; Wallingford; UK.Excoffier L; Laval G; Schneider S. 2005. Arlequin (ver. 3.0): Anintegrated software package for population genetics dataanalysis. Evolutionary Bioinformatics Online; 1; 47-50.[11] Figueroa C C; Simon J C; Gallic J F l; Prunier-Leterme N; BrionesL M; Dedryver C A; Niemeyer H M. 2005. Genetic structureand clonal diversity of an introduced pest in Chile; the cerealaphid Sitobion avenae. Heredity; 95; 24-33.[12] Guo W; Shen Z R; Li Z H; Gao L W. 2005. Migration andpopulation genetics of the grain aphid Macrosiphum miscanti(Takahashi) in relation to the geographic distance and geneflow. Progress in Natural Science; 15; 1000-1004.[13] Hebert P D N; Penton E H; Burns J M; Janzen D H; HallwachsW. 2004. Ten species in one: DNA barcoding reveals crypticspecies in the neotropical skipper butterfly Astraptesfulgerator. Proceedings of the National Academy of Sciencesof the USA; 101; 14812-14817.[14] Hu J; Zhang J L; Nardi F; Zhang R J. 2008. Population geneticstructure of the melon fly; Bactrocera cucurbitae (Diptera:Tephritidae); from China and Southeast Asia. Genetica; 134;319-324.[15] Jensen A B; Hansen L M; Eilenberg J. 2008. Grain aphidpopulation structure: no effect of fungal infections in a 2-year field study in Denmark. Agricultural and ForestEntomology; 10; 279-290.[16] Kimura M. 1980. A simple method for estimating evolutionaryrates of base substitutions through comparative studies ofnucleotide sequences. Journal of Molecular Evolution; 16;111-120.[17] Li J; Zhao F; Choi Y; Kim I; Sohn H; Jin B. 2006. Geneticvariation in the diamondback moth; Plutella xylostella(Lepidoptera: Yponomeutidae) in China inferred frommitochondrial COI gene sequence. European Journal ofEntomology; 103; 605-611.[18] Li L; Wang X F; Zhou G H. 2001. RAPD analyses of Sitobionavenae Fabricius population among different wheat areas innorthern China. Acta Phytophylacica Sinica; 28; 89-90. (inChinese)[19] Liu X D; Zhai B P; Zhang X X. 2004. Advance in the studies ofmigration of aphids. Entomological Knowledge; 41; 301-307.(in Chinese)[20] Liu Y P; Cao S X; Chen S Y; Yao Y G; Liu T Z. 2009. Geneticdiversity of Chinese domestic goat based on themitochondrial DNA sequence variation. Journal of AnimalBreeding and Genetics; 126; 80-89.[21] Llewellyn K S; Loxdale H D; Harrington R; Brookes C P; ClarkS J; Sunnucks P. 2003. Migration and genetic structure of thegrain aphid (Sitobion avenae) in Britain related to climate andclonal fluctuation as revealed using microsatellites. MolecularEcology; 12; 21-34.[22] Llewellyn K S; Loxdale H D; Harrington R; Clark S J; SunnucksP. 2004. Evidence for gene flow and local clonal selection infield populations of the grain aphid (Sitobion avenae) in Britainrevealed using microsatellites. Heredity; 93; 143-153.[23] Luo R W; Yang C L; Shang Y F; Li C S.1988. Migration and theoverwintering area study of Sitobion miscanthi. Journal ofPlant Protection; 15; 153-158. (in Chinese)[24] Papura D; Simon J C; Halkett F; Delmotte F; le Gallic J F;Dedryver C A. 2003. Predominance of sexual reproductionin Romanian populations of the aphid Sitobion avenaeinferred from phenotypic and genetic structure. Heredity;90; 397-404.[25] Rozas J; Sanchez-DelBarrio J C; Messeguer X; Rozas R. 2003.DnaSP; DNA polymorphism analyses by the coalescent andother methods. Bioinformatics; 19; 2496-2497.[26] Saitou N; Nei M. 1987. The neighbor-joining method: a newmethod for reconstructing phylogenetic trees. MolecularBiology and Evolution; 4; 406-425.[27] Simon C; Frati F; Beckenbach A; Crespi B; Liu H; Flook P. 1994.Evolution; weighting; and phylogenetic utility ofmitochondrial gene sequences and a compilation of conservedpolymerase chain reaction primers. Annals of theEntomological Society of America; 87; 651-701.[28] Simon J C; Baumann S; Sunnucks P; Hebert P D N; Pierre J S;Gallic J F l; Dedryver C A. 1999. Reproductive mode andpopulation genetic structure of the cereal aphid Sitobionavenae studied using phenotypic and microsatellite markers.Molecular Ecology; 8; 531-545.[29] Sunnucks P; Hales D. 1996. Numerous transposed sequences ofmitochondrial cytochrome oxidase I-II in aphids of the genusSitobion (Hemiptera: Aphididae). Molecular Biology andEvolution; 13; 510-524.[30] Tamura K; Dudley J; Nei M; Kumar S. 2007. MEGA4: Molecularevolutionary genetics analysis (MEGA) software ver. 4.0.Molecular Biology and Evolution; 24; 1596-1599.[31] Thompson J D; Higgins D G; Gibson T J. 1994. CLUSTAL W:improving the sensitivity of progressive multiple sequencealignment through sequence weighting; position-specific gappenalties and weight matrix choice. Nucleic Acids Research;22; 4673-4680.[32] Wright S. 1951. The genetic structure of populations. AnnualEugenics; 15; 323-354.[33] Xu Q; Liu R; Liu Y. 2009. Genetic population structure of theswimming crab; Portunus trituberculatus in the East ChinaSea based on mtDNA 16S rRNA sequences. Journal ofExperimental Marine Biology and Ecology; 371; 121-129.[34] Yang Y L. 1990. Progress in studies of migration and forecastingof Sitobion miscanthi. China Plant Protection; 3; 14-16. (inChinese) |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
