Abstract:

【Objective】To establish a simple electrophoresis characterization method for hapten-carrier protein bioconjugate. 【Method】 SDS-PAGE, nondenaturing agarose gel electrophoresis and high performance capillary electrophoresis were employed and compared with spectrum method. 【Result】 The result showed that SDS-PAGE was not a sensitive candidate method to differentiate carrier protein from bioconjugate when both proteins have less molecular weight difference, but nondenaturing gel electrophoresis was a better alternative, which could discriminate the free carrier protein and bioconjugate protein with different net charge numbers. High performance capillary zone electrophoresis was another powerful approach for hapten-carrier protein bioconjugate characterization. The information obtained from three electrophoresis was in accordance with the one from ultraviolet- visible spectrum scanning. 【Conclusion】 Three electrophoresis methods had different advantages and disadvantages, cost-saving and simple nondenaturing electrophoresis seemed to have more availability in most laboratories.

Key words: hapten-carrier protein bioconjugate, SDS-PAGE, nondenaturing agarose gel electrophoresis, capillary zone electrophoresis