2个新的鳞翅目OR83b类化感蛋白基因的克隆和序列分析

doi:10.3864/j.issn.0578-1752.2010.03.024

Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (3): 618-625 .doi: 10.3864/j.issn.0578-1752.2010.03.024

• RESEARCH NOTES • Previous Articles Next Articles

Molecular Cloning and Sequence Analysis of Two cDNA Genes of Two New Lepidoptera OR83b Orthologue Chemoreceptors

XIU Wei-ming, ZHANG Yi-fan, YANG Dian-lin, DONG Shuang-lin, LIU Yu-sheng

（农业部环境保护科研监测所/转基因生物生态环境安全监督检验测试中心）

Received:2009-05-04 Revised:2009-08-11 Online:2010-02-10 Published:2010-02-10
Contact: DONG Shuang-lin

Abstract

Abstract:

【Objective】 The aim of this study was to isolate the cDNA full sequence of an unusually highly conserved olfactory receptor (orthologue to the Drosophila melanogaster DOR83b) gene from the antennae of insects. 【Method】 The olfactory receptor genes were cloned by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. Bioinformatics methods were used to further analyze cDNA sequence obtained and putative amino acid sequence. 【Result】 Two cDNA full sequences of olfactory receptor (OR) gene were amplified from the antennae of male Spodoptera exigua and S. litura, and they were named SexiOR2 and SlitOR2. SexiOR2 and SlitOR2 contained 1 906 bp and 2 483 bp, and with deduced amino acid sequences of 473 residues. The sequence analysis indicated that the deduced amino acid sequences of the cDNA sequences shared high identity with OR83b orthologue chemoreceptor sequences from other previously reported moths. 【Conclusion】 It is inferred that the sequences obtained are cDNA sequences of OR83b orthologue chemoreceptor gene.

Key words: Spodoptera exigua, Spodoptera litura, olfactory receptor gene, sequence analysis

XIU Wei-ming,ZHANG Yi-fan,YANG Dian-lin,DONG Shuang-lin,LIU Yu-sheng
. Molecular Cloning and Sequence Analysis of Two cDNA Genes of Two New Lepidoptera OR83b Orthologue Chemoreceptors
[J].Scientia Agricultura Sinica, 2010, 43(3): 618-625 .

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