大豆(Glycine max L. Merri),异黄酮,HPLC," /> 大豆(Glycine max L. Merri),异黄酮,HPLC,"/> soybean (Glycine max L. Merri),isoflavone,HPLC,"/> <font face="Verdana">A Rapid HPLC Method for Determination of 12 Isoflavone Components in Soybean Seeds#br# </font>

Scientia Agricultura Sinica ›› 2009, Vol. 42 ›› Issue (7): 2491-2498 .doi: 10.3864/j.issn.0578-1752.2009.07.029

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

A Rapid HPLC Method for Determination of 12 Isoflavone Components in Soybean Seeds#br#

SUN Jun-ming, SUN Bao-li, HAN Fen-xia, YAN Shu-rong, YANG Hua, KIKUCHI Akio#br#   

  1. （中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程）

  • Received:2008-09-19 Revised:2009-03-05 Online:2009-07-10 Published:2009-07-10

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Abstract:

【Objective】 It is highly important and has utilization values to determine the isoflavone components by HPLC as it can provide bases for molecular assistant selection of isoflavone in soybean breeding. 【Method】 Based on the standard samples of 12 isoflavone components, the isoflavone components were analyzed using the determination of absorbance peaks method by HPLC. 【Result】 The results showed that there were different maximum absorbance wavelengths for the daidzein, glycitein, and genistein, which were 250 nm, 257 nm and 260 nm, respectively. A linear gradient elution of acetonitrile (13%-30%) containing 0.1% acetic acid using a mobile phase was applied on a YMC-C18 column at 35 ℃. The 12 isoflavone components were determined using the UV detector by HPLC. 【Conclusion】 It is concluded that this is a rapid, precise method which adapted to determine the large numbers of samples with microanalysis.

Key words: soybean (Glycine max L. Merri)')">soybean (Glycine max L. Merri), isoflavone, HPLC

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