Abstract:

【Objective】 To exploit the antifungal new active components from Spiraea alpina against phytopathogenic fungi. 【Method】 Different fractions of antifungal ethanol extract from the leaves of Spiraea alpina were obtained with different polarities of solvents such as petroleum ether, dichloromethane, ethyl acetate, ethanol and methanol. Guided by the inhibitory activities detection of Rhizoctonia solani, Gibberella zeae, Pyricularia oryzea and Exserohilum turcicum, the main antifungal component from the strongest antifungal fraction was separated by medium pressure preparation chromatogram and column chromatography, and the structure was analyzed by HR-MS, ultraviolet-vis, infra-red, 1H-NMR, 13C-NMR, 135-degree DEPT and HMBC spectrums. 【Result】 A new compound named 6-O-(3',4'-dihydroxy-2'-methylene-butyroyl)-1-O-trans-cinnamoyl-β-D-glucopyranose was found as a major fungal growth inhibitor, which performed an inhibitory rate to R. solani and E. turcicum, 86.7% and 63.2% at 0.3 mg?ml-1, respectively. 【Conclusion】 The EtOH extract from Spiraea. alpina had some inhibitory activities on R. solani and E. turcicum, and studies on Spiraea. alpina would give some more new fungitoxic metabolites.

Key words: Spiraea alpine')">Spiraea alpine, Rhizoctonia solani, Exserohilum turcicum, antifungal activity, separation, identification