免疫分离技术在生鲜乳致病菌检测中的研究进展

doi:10.3864/j.issn.0578-1752.2015.S.006

Abstract

Abstract: Microbial community in raw milk is an important factor influencing quality and safety of the dairy products, as it reflects hygienic conditions during milking and storage of raw milk in the bulk tank. Foodborne pathogens of high public health concern, including Staphylococcus aureus, Listeria monocytogenes, Salmonella, Bacillus cereus and Escherichia coli, seriously affected human health. Traditional culture-based methods, ELISA, LAMP and PCR are used for detection of pathogens. Detection sensitivity of existing methods are low without pre-enrichment, limiting the methods directly used for rapid detection of pathogen in raw milk. Immunomagnetic separation has been suggested to reduced the analysis time and improved the sensitivity detection of pathogenic microorganisms. The methods were successfully used to detect Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, Escherichia coli, Mycobacterium avium, Campylobacter jejuni, Helicobacter pylori, Campylobacter spp, Yersinia spp and Chlamydia trachomatis. Immunomagnetic separation combined with spectrophotometer, Chrom agar, real-time PCR, fluorescence immunochr omatographic assay or colloidal gold lateral flow assay were effective for detection and isolation of Escherichia coli. Immunomagnetic separation combined with Chrom agar, colorimetric detection, fluorescence immunochr omatographic assay and LAMP were effective for detection and isolation of Staphylococcus aureus. Immunomagnetic separation combined with qPCR, ELISA, quantum dots and LAMP were effective for detection and isolation of Salmonella spp. Immunomagnetic separation combined with flow cytometry, selective medium and real time PCR were effective for detection and isolation of Listeria monocytogenes. Recent progress in the application of immunomagnetic separation technology for the mainly pathogens in raw milk was reviewed, and the problems and development trends about immunomagnetic separation were analyzed and discussed in the paper.

Key words: immunomagnetic separation, raw milk, pathogen detection

LAN Xin-yi, LIU Hui-min, ZHENG Nan, LI Fa-di, WANG Jia-qi. Immunomagnetic Separation for Foodborne Pathogen Detection in Raw Milk[J].Scientia Agricultura Sinica, 2015, 48(S): 49-57.

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