Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (02): 425-427 .doi: 10.3864/j.issn.0578-1752.at-2004-2075

• RESEARCH NOTES • Previous Articles     Next Articles

Establishment and Application of Detection of Prune Dwarf Virus (PDV) by RT-PCR

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  1. 内蒙古农业大学农学院
  • Received:2004-02-03 Revised:1900-01-01 Online:2005-02-10 Published:2005-02-10

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Abstract: A pair of primers was synthesized based on the nucleotide sequence of the coat protein gene of prune dwarf virus (PDV).The expected size 172bp was amplified by RT-PCR from the infected samples, while no amplified products from the healthy samples.The amplified products were cloned and sequenced. The result showed that the detection system was stable.Thus a rapid, sensitive and accurate method for PDV identification and detection by RT-PCR has been built up and were used to detect whether some fruit trees in orchards were infected by PDV or not.

Key words: Prune dwarf virus, RT-PCR, Cloning and sequencing

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