Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (9): 2053-2061 .doi: 10.3864/j.issn.0578-1752.at-2006-7623

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Study on SLA Class I-3 Alleles for Inbreeding WZSP

SUN Jun-li MU Yu-lian LIU xiao-lin WANG Su-rong   

  1. 中国农业科学院畜牧研究所
  • Received:2006-05-25 Revised:2006-10-09 Online:2007-09-10 Published:2007-09-10

PDF

1440

Abstract: To elucidate the structure of SLAⅠ3 alleles on inbred line of Wuzhishan pig（WZSP） population. RT-PCR products of the partial exon1, completed exon2 and partial exon3 of the SLA classical I class 3 gene were successed to amplify in 32 WZSPs. According to genealogy and amplification results, PCR products of 16 WZSPs were selected to clone and sequence. 10 different nucleotide sequences were obtained. The sequences data and their coden amino acids sequences were compared with all sequences of SLAⅠ3 gene in GenBank, respectively. It was found that these sequences were different in comparison with each other or with those of published sequences of SLAⅠ3 gene, but there were very few variation among them. The data of comparing the amino acids sequences indicated that most of these sites are conserved among the related molecules between porcine and human. Meanwhile, construction of a phylogenetic tree using the nucleotide sequences of those 23 alleles and 1 HLA A gene indicated that WZSP population own its unique resource.

Key words: Inbreding Wuzhishan pig, Leucocyte antigen, SLA classical I class 3 gene, RT-PCR

[1] JIANG Feng, WU ChunYan, WANG YiHao, YANG ZeZhong, GONG Cheng, LUO Chen. Identification and Expression Analysis of the Fatty Acid Elongase Gene Family in Bemisia tabaci MED [J]. Scientia Agricultura Sinica, 2026, 59(4): 793-806.
[2] XU DuoDuo, DU QianQian, ZHAO LiXiang, LI Yan, HUANG Gan, LI YongHua, LU JiuXing. Genome-Wide Analysis of AP2/ERF Transcription Factors in Peony [J]. Scientia Agricultura Sinica, 2025, 58(23): 5031-5045.
[3] WANG ChengZe, ZHANG Yan, FU Wei, JIA JingZhe, DONG JinGao, SHEN Shen, HAO ZhiMin. Bioinformatics and Expression Pattern Analysis of Maize ACO Gene Family [J]. Scientia Agricultura Sinica, 2024, 57(7): 1308-1318.
[4] REN CaiXia, LIU Lin, LIU ShengXue, BU Fang DI, XIANG BenChun, ZHENG YinYing, CUI BaiMing. Complete Genome Sequence Analysis and Infectious Clone Construction of Mume Virus A Peach Isolate pp in Xinjiang [J]. Scientia Agricultura Sinica, 2024, 57(19): 3810-3822.
[5] XIAO Tao, LI Hui, LUO Wei, YE Tao, YU Huan, CHEN YouBo, SHI YuShi, ZHAO DePeng, WU Yun. Screening of Candidate Genes for Green Shell Egg Shell Color Traits in Chishui Black Bone Chicken Based on Transcriptome Sequencing [J]. Scientia Agricultura Sinica, 2023, 56(8): 1594-1605.
[6] SU Qi, TANG YaFei, SHE XiaoMan, LAN GuoBing, YU Lin, WU ZhengWei, LI ZhengGang, HE ZiFu. Identification of Viruses Infecting Bottle Gourd in Guangdong Province and Establishment of Multiplex RT-PCR Detection Method [J]. Scientia Agricultura Sinica, 2023, 56(23): 4684-4695.
[7] LIAO FuRong, CHEN HongYun, SHEN JianGuo, FANG ZhiPeng, HUANG PengYing, CHEN Qing, LIN LingLing, HONG Jun. Development of a Degenerate Primer RT-PCR Assay for Detection of Carmovirus [J]. Scientia Agricultura Sinica, 2023, 56(12): 2288-2301.
[8] WANG YiDan,YANG FaLong,CHEN DiShi,XIANG Hua,REN YuPeng. One-Step Multiple TaqMan Real-time RT-PCR for Simultaneous Detection of Swine Diarrhea Viruses [J]. Scientia Agricultura Sinica, 2023, 56(1): 179-192.
[9] WANG SiTong,CHEN Yan,LUO YuJia,YANG YuanYuan,JIANG ZhiYang,JIANG XinYi,ZHONG Fan,CHEN Hao,XU HongXing,WU Yan,DUAN HongXia,TANG Bin. Effect of Three Novel Compounds on Trehalose and Chitin Metabolism and Development of Spodoptera frugiperda [J]. Scientia Agricultura Sinica, 2022, 55(8): 1568-1578.
[10] XIE LiXue,ZHANG XiaoYan,ZHANG LiJie,ZHENG Shan,LI Tao. Complete Genome Sequence Characteristics and TC-RT-PCR Detection of East Asian Passiflora Virus Infecting Passiflora edulis [J]. Scientia Agricultura Sinica, 2022, 55(22): 4408-4418.
[11] KANG JunMei,ZHANG QiaoYan,JIANG Xu,WANG Zhen,ZHANG TieJun,LONG RuiCai,CUI HuiTing,YANG QingChuan. Cloning MsSQE1 from Alfalfa and Functional Analysis in Saponin Synthesis [J]. Scientia Agricultura Sinica, 2020, 53(2): 247-260.
[12] XU JianJian,WANG YanJiao,DUAN Yu,MA ZhiMin,BIN Yu,ZHOU ChangYong,SONG Zhen. Construction of Genome-Length cDNA of Citrus Vein Enation Virus and Identification of Its Infectivity [J]. Scientia Agricultura Sinica, 2020, 53(18): 3707-3715.
[13] ZHANG DaoWei,KANG Kui,YU YaYa,KUANG FuPing,PAN BiYing,CHEN Jing,TANG Bin. Characteristics and Immune Response of Prophenoloxidase Genes in Sogatella furcifera [J]. Scientia Agricultura Sinica, 2020, 53(15): 3108-3119.
[14] LIU YiRan,ZHANG Hong,JIN JiSu,ZHOU ZhongShi,GUO JianYing. Identification and Expression Analysis of the Halloween Gene Family in Agasicles hygrophila [J]. Scientia Agricultura Sinica, 2020, 53(10): 2009-2019.
[15] DING YanJuan,LIU YongKang,LUO YuJia,DENG YingMei,XU HongXing,TANG Bin,XU CaiDi. Potential Functions of Nilaparvata lugens GSK-3 in Regulating Glycogen and Trehalose Metabolism [J]. Scientia Agricultura Sinica, 2019, 52(7): 1237-1246.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd