长期有机无机肥配施对潮土细菌群落结构、生态网络及关键物种的影响

doi:10.3864/j.issn.0578-1752.2026.02.010

Abstract

Abstract:

【Objective】 Based on the long-term fertilization station in fluvo-aquic soil region, this study investigated how bacterial community structure, ecological network, and key species in response to fertilization. 【Method】 Based on the long-term experiment started from 1990, soil was sampled after wheat maturity in 2023, and high-throughput sequencing and ecological network analysis were used to examine dynamics in soil organic carbon (SOC) and other nutrients, enzyme activity, bacterial community composition, ecological network and stochastic process under four treatments: no fertilization (CK), mineral nitrogen (N), phosphorus (P), and potassium (K) (NPK), NPK+straw (NPKS), and NPK+manure (NPKM). 【Result】 The combined application of organic and inorganic fertilizers significantly increased SOC and other soil nutrients, as well as soil enzyme activity. Compared with CK, SOC content under NPKS and NPKM increased by 52.1% and 81.9%, respectively, and particulate carbon increased by 60.6% and 137.4%, respectively, and easily oxidized organic carbon increased by 45.3% and 63.4%, respectively; additionally, β-N-acetylglucosaminidase activity increased by 7.2% and 12.6%, respectively, while alkaline phosphatase activity increased by 166.4% and 216.2% (P<0.05), respectively. Notably, β-1,4-glucosidase activity was the highest under NPKS (63.82 μmol·g^-1·d^-1). In terms of bacterial diversity, α-diversity significantly decreased under NPKS compared with CK, with reductions of 5.4%, 5.2%, and 2.6% in the Ace, Chao1, and Shannon indices, respectively (P<0.05). Fertilization treatment alerted bacterial community structure, while NPKS and NPKM exhibited similar compositions. Compared with CK, NPKS significantly reduced the relative abundance of Chloroflexi, Gemmatimonadota, and Methylomirabilota, while NPKM significantly increased the relative abundance of Bacteroidota (P<0.05). Redundancy analysis identified ammonium nitrogen (NH₄⁺-N), nitrate nitrogen (NO₃^--N), and organic carbon (SOC) as the primary environmental factors shaping microbial community structure. Network analysis showed, compared with CK, NPKM increased the complexity, stability of the bacterial community network and the proportion of positive correlations between species. Furthermore, both NPKS and NPKM significantly enhanced the relative abundances of eight keystone taxa, including members of Actinobacteriota (order Microtrichales), Chloroflexi (order Thermomicrobiales), Bacteroidota (orders Chitinophagales and Cytophagales), Myxococcota (uncultured order bacteriap25), and Proteobacteria (order Burkholderiales) (P<0.05). These keystone taxa were closely associated with soil carbon, nitrogen, phosphorus, and other material cycling, as well as plant growth promotion. Partial least squares path modeling suggested that fertilization did not directly impact key species but exerted an indirect influence by significantly affecting soil pH, regulating community composition, and increasing soil nutrient availability. 【Conclusion】 Long-term combined application of organic and inorganic fertilizers enhanced soil nutrient content and extracellular enzyme activity, regulated microbial community composition and structure, affected bacterial network complexity and stability, and increased the relative abundance of key species involved in soil nutrient cycling and material transformation. These findings provided valuable insights into the interactions between soil properties and microbial communities under long-term fertilization, contributing to a deeper understanding of bacterial community dynamics and key species in agricultural ecosystems.

Key words: long-term fertilization, fluvo-aquic soil, bacterial community structure, network analysis, key species

WANG RenZhuo, LI YueYing, HUANG ShaoMin, JIANG GuiYing, ZHANG Qi, LIU ChaoLin, YANG Jin, WANG MengRu, WANG BeiBei, LIU Fang, GUO DouDou, JIE XiaoLei, SONG Lian, LIU ShiLiang. Effects of Long-Term Combination of Organic and Inorganic Fertilizers on Bacterial Community Structure, Ecological Network, and Key Species in Fluvo-Aquic Soil[J].Scientia Agricultura Sinica, 2026, 59(2): 354-367.

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References 61

[1]	JI Y X, ZHAO Y, HAN X Z, CHEN X, YAN J, LU X C, ZHU Y C, ZOU W X. Effect of long-term fertilization practices on the stability of soil organic matter in the northeast black soil region in China. Agronomy, 2024, 14(10): 2272. doi: 10.3390/agronomy14102272
[2]	ABDO A I, SUN D L, SHI Z J, ABDEL-FATTAH M K, ZHANG J E, KUZYAKOV Y. Conventional agriculture increases global warming while decreasing system sustainability. Nature Climate Change, 2024, 15(1): 110-117. doi: 10.1038/s41558-024-02170-4
[3]	HU Z K, DELGADO-BAQUERIZO M, FANIN N, CHEN X Y, ZHOU Y, DU G Z, HU F, JIANG L, HU S J, LIU M Q. Nutrient- induced acidification modulates soil biodiversity-function relationships. Nature Communications, 2024, 15: 2858. doi: 10.1038/s41467-024-47323-3
[4]	SHI T S, COLLINS S L, YU K L, PEÑUELAS J, SARDANS J, LI H L, YE J S. A global meta-analysis on the effects of organic and inorganic fertilization on grasslands and croplands. Nature Communications, 2024, 15: 3411. doi: 10.1038/s41467-024-47829-w
[5]	HERNANDEZ D J, DAVID A S, MENGES E S, SEARCY C A, AFKHAMI M E. Environmental stress destabilizes microbial networks. The ISME Journal, 2021, 15(6): 1722-1734. doi: 10.1038/s41396-020-00882-x
[6]	PHILIPPOT L, CHENU C, KAPPLER A, RILLIG M C, FIERER N. The interplay between microbial communities and soil properties. Nature Reviews Microbiology, 2023, 22(4): 226-239. doi: 10.1038/s41579-023-00980-5 pmid: 37863969
[7]	TANG S, MA Q X, MARSDEN K A, CHADWICK D R, LUO Y, KUZYAKOV Y, WU L H, JONES D L. Microbial community succession in soil is mainly driven by carbon and nitrogen contents rather than phosphorus and sulphur contents. Soil Biology and Biochemistry, 2023, 180: 109019. doi: 10.1016/j.soilbio.2023.109019
[8]	LIU B, XIA H, JIANG C C, RIAZ M, YANG L, CHEN Y F, FAN X P, XIA X G. 14 years applications of chemical fertilizers and crop straw effects on soil labile organic carbon fractions, enzyme activities and microbial community in rice-wheat rotation of middle China. Science of the Total Environment, 2022, 841: 156608. doi: 10.1016/j.scitotenv.2022.156608
[9]	XIE Z H, YU Z H, LI Y S, WANG G H, LIU X B, TANG C X, LIAN T X, ADAMS J, LIU J J, LIU J D, HERBERT S J, JIN J. Soil microbial metabolism on carbon and nitrogen transformation links the crop-residue contribution to soil organic carbon. NPJ Biofilms and Microbiomes, 2022, 8: 14. doi: 10.1038/s41522-022-00277-0 pmid: 35365687
[10]	LIU S B, WANG J Y, PU S Y, BLAGODATSKAYA E, KUZYAKOV Y, RAZAVI B S. Impact of manure on soil biochemical properties: A global synthesis. Science of the Total Environment, 2020, 745: 141003. doi: 10.1016/j.scitotenv.2020.141003
[11]	ZHAO N, WANG X Q, MA J, LI X H, CAO J F, ZHOU J, WU L M, ZHAO P Y, CAO W D. Co-incorporating green manure and crop straw increases crop productivity and improves soil quality with low greenhouse-gas emissions in a crop rotation. The Crop Journal, 2024, 12(4): 1233-1241. doi: 10.1016/j.cj.2024.07.002
[12]	LI X Y, LI B, CHEN L, LIANG J Y, HUANG R, TANG X Y, ZHANG X, WANG C Q. Partial substitution of chemical fertilizer with organic fertilizer over seven years increases yields and restores soil bacterial community diversity in wheat-rice rotation. European Journal of Agronomy, 2022, 133: 126445. doi: 10.1016/j.eja.2021.126445
[13]	ZHU L Y, LUAN L, CHEN Y, WANG X Y, ZHOU S G, ZOU W X, HAN X R, DUAN Y H, ZHU B, LI Y, et al. Community assembly of organisms regulates soil microbial functional potential through dual mechanisms. Global Change Biology, 2024, 30(2): e17160. doi: 10.1111/gcb.v30.2
[14]	GUO Z B, WAN S X, HUA K K, YIN Y, CHU H Y, WANG D Z, GUO X S. Fertilization regime has a greater effect on soil microbial community structure than crop rotation and growth stage in an agroecosystem. Applied Soil Ecology, 2020, 149: 103510. doi: 10.1016/j.apsoil.2020.103510
[15]	LIU C, ZHOU Z Y, SUN S, ZHANG Q, SUN S Q, RAVANBAKHSH M, WEI Z, LI R, WANG S M, et al. Investigating protistan predators and bacteria within soil microbiomes in agricultural ecosystems under organic and chemical fertilizer applications. Biology and Fertility of Soils, 2024, 60(7): 1009-1024. doi: 10.1007/s00374-024-01845-6
[16]	ZHAO Z Y, MA Y T, ZHANG A, CHEN Y M, ZHENG Z X, ZHENG W, ZHAI B N. Response of apple orchard bacteria co-occurrence network pattern to long-term organic fertilizer input. Applied Soil Ecology, 2023, 191: 105035. doi: 10.1016/j.apsoil.2023.105035
[17]	LI K, XING X Y, WANG S B, LIAO R J, HASSAN M U, AAMER M, BARBANTI L, WEN T W, XU H F. Organic fertilisation enhances network complexity among bacteria, fungi, and protists by improving organic matter and phosphorus in acidic agricultural soils. European Journal of Soil Biology, 2024, 122: 103649. doi: 10.1016/j.ejsobi.2024.103649
[18]	WANG L, WANG J, TANG Z H, WANG J D, ZHANG Y C. Long- term organic fertilization reshapes the communities of bacteria and fungi and enhances the activities of C- and P-cycling enzymes in calcareous alluvial soil. Applied Soil Ecology, 2024, 194: 105204. doi: 10.1016/j.apsoil.2023.105204
[19]	ZHANG Z M, YAN J, HAN X Z, ZOU W X, CHEN X, LU X C, FENG Y T. Labile organic carbon fractions drive soil microbial communities after long-term fertilization. Global Ecology and Conservation, 2021, 32: e01867.
[20]	LI K, CHEN A L, SHENG R, HOU H J, ZHU B L, WEI W X, ZHANG W Z. Long-term chemical and organic fertilization induces distinct variations of microbial associations but unanimous elevation of soil multifunctionality. Science of the Total Environment, 2024, 931: 172862. doi: 10.1016/j.scitotenv.2024.172862
[21]	WANG J L, LIU K L, ZHAO X Q, ZHANG H Q, LI D, LI J J, SHEN R F. Balanced fertilization over four decades has sustained soil microbial communities and improved soil fertility and rice productivity in red paddy soil. Science of the Total Environment, 2021, 793: 148664. doi: 10.1016/j.scitotenv.2021.148664
[22]	DĂMĂTÎRCĂ C, MORETTI B, BERTORA C, FERRARINI A, LERDA C, MANIA I, CELI L, GORRA R, ZAVATTARO L. Residue incorporation and organic fertilisation improve carbon and nitrogen turnover and stabilisation in maize monocropping. Agriculture, Ecosystems & Environment, 2023, 342: 108255. doi: 10.1016/j.agee.2022.108255
[23]	SHI Y, DELGADO-BAQUERIZO M, LI Y T, YANG Y F, ZHU Y G, PEÑUELAS J, CHU H Y. Abundance of kinless hubs within soil microbial networks are associated with high functional potential in agricultural ecosystems. Environment International, 2020, 142: 105869. doi: 10.1016/j.envint.2020.105869
[24]	郭伟, 周云鹏, 陈美淇, 李丹丹, 王青霞, 周谈坛, 赵炳梓. 秸秆与有机无机肥配施对潮土关键微生物及小麦产量的影响. 土壤学报, 2024, 61(4): 1134-1146.
	GUO W, ZHOU Y P, CHEN M Q, LI D D, WANG Q X, ZHOU T T, ZHAO B Z. Effects of combined application of straw and organic-inorganic fertilizers on key microorganisms and wheat yield in fluvo-aquic soil. Acta Pedologica Sinica, 2024, 61(4): 1134-1146. (in Chinese)
[25]	鲍士旦. 土壤农化分析. 3版. 北京: 中国农业出版社, 2000: 39-57.
	BAO S D. Soil and Agricultural Chemistry Analysis. 3rd ed. Beijing: China Agriculture Press, 2000: 39-57. (in Chinese)
[26]	HE H, PENG M W, LU W D, RU S B, HOU Z N, LI J H. Organic fertilizer substitution promotes soil organic carbon sequestration by regulating permanganate oxidizable carbon fractions transformation in oasis wheat fields. Catena, 2023, 221: 106784. doi: 10.1016/j.catena.2022.106784
[27]	LIU C S, ZHAO D F, MA W J, GUO Y D, WANG A J, WANG Q L, LEE D J. Denitrifying sulfide removal process on high-salinity wastewaters in the presence of Halomonas sp. Applied Microbiology and Biotechnology, 2016, 100(3): 1421-1426. doi: 10.1007/s00253-015-7039-6
[28]	LIU C, LI C N, JIANG Y Q, ZENG R J, YAO M J, LI X Z. A guide for comparing microbial co-occurrence networks. iMeta, 2023, 2(1): e71. doi: 10.1002/imt2.71 pmid: 38868345
[29]	FRIEDMAN J, ALM E J. Inferring correlation networks from genomic survey data. PLoS Computational Biology, 2012, 8(9): e1002687. doi: 10.1371/journal.pcbi.1002687
[30]	WEN T, NIU G Q, CHEN T, SHEN Q R, YUAN J, LIU Y X. The best practice for microbiome analysis using R. Protein & Cell, 2023, 14(10): 713-725.
[31]	ZHANG Z M, HE P, HAO X X, LI L J. Long-term mineral combined with organic fertilizer supports crop production by increasing microbial community complexity. Applied Soil Ecology, 2023, 188: 104930. doi: 10.1016/j.apsoil.2023.104930
[32]	GUIMERÀ R, NUNES AMARAL L A. Functional cartography of complex metabolic networks. Nature, 2005, 433(7028): 895-900. doi: 10.1038/nature03288
[33]	CHENU C, ANGERS D A, BARRÉ P, DERRIEN D, ARROUAYS D, BALESDENT J. Increasing organic stocks in agricultural soils: Knowledge gaps and potential innovations. Soil and Tillage Research, 2019, 188: 41-52. doi: 10.1016/j.still.2018.04.011
[34]	YANG X, WANG B R, FAKHER A, AN S S, KUZYAKOV Y. Contribution of roots to soil organic carbon: From growth to decomposition experiment. Catena, 2023, 231: 107317. doi: 10.1016/j.catena.2023.107317
[35]	GARCÍA-ORENES F, ROLDÁN A, MORUGÁN-CORONADO A, LINARES C, CERDÀ A, CARAVACA F. Organic fertilization in traditional Mediterranean grapevine orchards mediates changes in soil microbial community structure and enhances soil fertility. Land Degradation & Development, 2016, 27(6): 1622-1628. doi: 10.1002/ldr.v27.6
[36]	SICILIANO S D, PALMER A S, WINSLEY T, LAMB E, BISSETT A, BROWN M V, VAN DORST J, JI M K, FERRARI B C, GROGAN P, CHU H Y, SNAPE I. Soil fertility is associated with fungal and bacterial richness, whereas pH is associated with community composition in polar soil microbial communities. Soil Biology and Biochemistry, 2014, 78: 10-20. doi: 10.1016/j.soilbio.2014.07.005
[37]	HU X J, GU H D, LIU J J, WEI D, ZHU P, CUI X A, ZHOU B K, CHEN X L, JIN J, LIU X B, WANG G H. Metagenomic strategies uncover the soil bioavailable phosphorus improved by organic fertilization in Mollisols. Agriculture, Ecosystems & Environment, 2023, 349: 108462. doi: 10.1016/j.agee.2023.108462
[38]	LIU C Z, HAN X Z, LU X C, YAN J, CHEN X, ZOU W X. Response of soil enzymatic activity to pore structure under inversion tillage with organic materials incorporation in a Haplic Chernozem. Journal of Environmental Management, 2024, 370: 122421. doi: 10.1016/j.jenvman.2024.122421
[39]	LIU X Q, LIU H R, ZHANG Y S, LIU C R, LIU Y N, LI Z H, ZHANG M C. Organic amendments alter microbiota assembly to stimulate soil metabolism for improving soil quality in wheat-maize rotation system. Journal of Environmental Management, 2023, 339: 117927. doi: 10.1016/j.jenvman.2023.117927
[40]	ABAY P, GONG L, LUO Y, ZHU H Q, DING Z L. Soil extracellular enzyme stoichiometry reveals the nutrient limitations in soil microbial metabolism under different carbon input manipulations. Science of the Total Environment, 2024, 913: 169793. doi: 10.1016/j.scitotenv.2023.169793
[41]	KOBIERSKI M, BARTKOWIAK A, LEMANOWICZ J, PIEKARCZYK M. Impact of poultry manure fertilization on chemical and biochemical properties of soils. Plant, Soil and Environment, 2017, 63(12): 558-563. doi: 10.17221/668/2017-PSE
[42]	BÜNEMANN E K, BONGIORNO G, BAI Z G, CREAMER R E, DE DEYN G, DE GOEDE R, FLESKENS L, GEISSEN V, KUYPER T W, MÄDER P, et al. Soil quality—A critical review. Soil Biology and Biochemistry, 2018, 120: 105-125.
[43]	ZHOU X, TAHVANAINEN T, MALARD L, CHEN L, PÉREZ- PÉREZ J, BERNINGER F. Global analysis of soil bacterial genera and diversity in response to pH. Soil Biology and Biochemistry, 2024, 198: 109552. doi: 10.1016/j.soilbio.2024.109552
[44]	马垒, 李燕, 魏建林, 周晓琳, 李子双, 李国生, 吴小宾, 刘兆辉, 谭德水. 连续施用化肥及秸秆还田对潮土酶活性、细菌群落和分子生态网络的影响. 植物营养与肥料学报, 2022, 28(8): 1353-1363.
	MA L, LI Y, WEI J L, ZHOU X L, LI Z S, LI G S, WU X B, LIU Z H, TAN D S. Effects of continuous chemical fertilizer application and straw returning on soil enzyme activity, bacterial community and co-occurrence patterns in a fluvo-aquic soil. Journal of Plant Nutrition and Fertilizers, 2022, 28(8): 1353-1363. (in Chinese)
[45]	BEI S K, ZHANG Y L, LI T T, CHRISTIE P, LI X L, ZHANG J L. Response of the soil microbial community to different fertilizer inputs in a wheat-maize rotation on a calcareous soil. Agriculture, Ecosystems & Environment, 2018, 260: 58-69. doi: 10.1016/j.agee.2018.03.014
[46]	CEDERLUND H, WESSÉN E, ENWALL K, JONES C M, JUHANSON J, PELL M, PHILIPPOT L, HALLIN S. Soil carbon quality and nitrogen fertilization structure bacterial communities with predictable responses of major bacterial phyla. Applied Soil Ecology, 2014, 84: 62-68. doi: 10.1016/j.apsoil.2014.06.003
[47]	练金山, 王慧颖, 徐明岗, 魏文良, 段英华, 刘树堂. 长期施用有机肥潮土细菌的多样性及功能预测. 植物营养与肥料学报, 2021, 27(12): 2073-2082.
	LIAN J S, WANG H Y, XU M G, WEI W L, DUAN Y H, LIU S T. Diversity and function prediction of bacteria community in fluvo- aquic soils as affected by long-term organic fertilization. Journal of Plant Nutrition and Fertilizers, 2021, 27(12): 2073-2082. (in Chinese)
[48]	LI X M, CHEN Q L, HE C, SHI Q, CHEN S C, REID B J, ZHU Y G, SUN G X. Organic carbon amendments affect the chemodiversity of soil dissolved organic matter and its associations with soil microbial communities. Environmental Science & Technology, 2019, 53(1): 50-59. doi: 10.1021/acs.est.8b04673
[49]	CHEN L, LIU Q, DU H L, CUI J X, CHEN Y Q. Organic materials return suppressed soil N₂O emissions by changing the composition instead of abundance of denitrifying microbial community. Applied Soil Ecology, 2024, 204: 105759. doi: 10.1016/j.apsoil.2024.105759
[50]	CAO X Q, LIU J L, ZHANG L L, MAO W J, LI M, WANG H, SUN W L. Response of soil microbial ecological functions and biological characteristics to organic fertilizer combined with biochar in dry direct-seeded paddy fields. Science of the Total Environment, 2024, 948: 174844. doi: 10.1016/j.scitotenv.2024.174844
[51]	SU Y, LÜ J L, YU M, MA Z H, XI H, KOU C L, HE Z C, SHEN A L. Long-term decomposed straw return positively affects the soil microbial community. Journal of Applied Microbiology, 2020, 128(1): 138-150. doi: 10.1111/jam.14435 pmid: 31495045
[52]	吴宪, 张婷, 王蕊, 王欣奕, 赵建宁, 王丽丽, 杨殿林, 李刚, 修伟明. 化肥减量配施有机肥和秸秆对华北潮土团聚体分布及稳定性的影响. 生态环境学报, 2020, 29(5): 933-941. doi: 10.16258/j.cnki.1674-5906.2020.05.009
	WU X, ZHANG T, WANG R, WANG X Y, ZHAO J N, WANG L L, YANG D L, LI G, XIU W M. Effects of chemical fertilizer reduction combined with application of organic fertilizer and straw on fluvo-aquic soil aggregate distribution and stability in North China. Ecology and Environmental Sciences, 2020, 29(5): 933-941. (in Chinese)
[53]	YANG L H, SUN R H, LI J G, ZHAI L M, CUI H L, FAN B Q, WANG H Y, LIU H B. Combined organic-inorganic fertilization builds higher stability of soil and root microbial networks than exclusive mineral or organic fertilization. Soil Ecology Letters, 2022, 5(2): 220142. doi: 10.1007/s42832-022-0142-6
[54]	张周, 陈瑞蕊, 王晓婷, 俞冰倩, 林先贵, 冯有智. 干旱扰动下长期不同施肥潮土微生物群落稳定性研究. 土壤学报, 2024, 61(1): 211-222.
	ZHANG Z, CHEN R R, WANG X T, YU B Q, LIN X G, FENG Y Z. Soil microbial community stability of different fertilization strategies under drought disturbance. Acta Pedologica Sinica, 2024, 61(1): 211-222. (in Chinese)
[55]	WANG J F, YANG X Y, HUANG S M, WU L, CAI Z J, XU M G. Long-term combined application of organic and inorganic fertilizers increases crop yield sustainability by improving soil fertility in maize-wheat cropping systems. Journal of Integrative Agriculture, 2025, 24(1): 290-305.
[56]	WANG E Z, LIN X L, TIAN L, WANG X G, JI L, JIN F, TIAN C J. Effects of short-term rice straw return on the soil microbial community. Agriculture, 2021, 11(6): 561. doi: 10.3390/agriculture11060561
[57]	张艳楠, 李艳丽, 王磊, 陈金海, 胡煜, 付小花, 乐毅全. 崇明东滩不同演替阶段湿地土壤有机碳汇聚能力的差异性及其微生物机制. 农业环境科学学报, 2012, 31(3): 631-637.
	ZHANG Y N, LI Y L, WANG L, CHEN J H, HU Y, FU X H, LE Y Q. Variability in organic carbon storage capability of soils at different successional stages in Chongming Dongtan wetland and its microbial mechanism. Journal of Agro-Environment Science, 2012, 31(3): 631-637. (in Chinese)
[58]	梁幸, 孔令露. 噬几丁质菌属及其天然产物研究进展. 工业微生物, 2023, 53(1): 22-25.
	LIANG X, KONG L L. On the research progress of Chitinophaga species and its natural products. Industrial Microbiology, 2023, 53(1): 22-25. (in Chinese)
[59]	黄瑞林, 张娜, 孙波, 梁玉婷. 典型农田根际土壤伯克霍尔德氏菌群落结构及其多样性. 土壤学报, 2020, 57(4): 975-985.
	HUANG R L, ZHANG N, SUN B, LIANG Y T. Community structure of Burkholderiales and its diversity in typical maize rhizosphere soil. Acta Pedologica Sinica, 2020, 57(4): 975-985. (in Chinese)
[60]	SONG M Y, LI J F, GAO L H, TIAN Y Q. Comprehensive evaluation of effects of various carbon-rich amendments on overall soil quality and crop productivity in degraded soils. Geoderma, 2023, 436: 116529. doi: 10.1016/j.geoderma.2023.116529
[61]	KOPPRIO G A, NEOGI S B, RASHID H, ALONSO C, YAMASAKI S, KOCH B P, GÄRDES A, LARA R J. Vibrio and bacterial communities across a pollution gradient in the Bay of Bengal: unraveling their biogeochemical drivers. Frontiers in Microbiology, 2020, 11: 594. doi: 10.3389/fmicb.2020.00594

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处理 Treatment	Ace指数 Ace index	Chao1指数 Chao1 index	Shannon指数 Shannon index	Coverage指数 Coverage index
CK	4986.56±89.04a	4848.62±87.69a	6.98±0.04a	0.98
NPK	4961.90±38.23a	4783.47±16.68a	6.96±0.04a	0.98
NPKS	4718.30±71.41b	4562.25±73.74b	6.81±0.03b	0.98
NPKM	4967.40±28.56a	4809.90±44.68a	6.95±0.02a	0.98

细菌门类Bacterial Phyla	CK	NPK	NPKS	NPKM
变形菌门Proteobacteria	18.97±2.92a	23.01±2.44a	21.31±3.33a	26.11±5.25a
放线菌门Actinobacteriota	19.79±2.35a	22.10±0.79a	20.39±2.41a	22.61±1.73a
酸杆菌门Acidobacteriota	22.08±4.23a	18.62±4.32a	22.96±6.48a	14.67±6.15a
绿弯菌门Chloroflexi	12.92±0.87a	11.44±1.16ab	9.89±0.28b	10.95±2.06ab
厚壁菌门Firmicutes	4.75±0.76a	3.72±0.86a	5.93±0.63a	5.07±1.78a
拟杆菌门Bacteroidota	1.99±0.48b	3.16±0.47ab	3.50±0.58ab	4.51±1.41a
芽单胞菌门Gemmatimonadota	3.42±0.12a	3.67±0.13a	2.77±0.12b	3.20±0.46ab
黏球菌门Myxococcota	2.65±0.39a	2.56±0.39a	2.38±0.31a	2.34±0.43a
甲基微菌门Methylomirabilota	2.50±0.36a	2.07±0.07b	1.74±0.12b	1.90±0.06b

Effects of Long-Term Combination of Organic and Inorganic Fertilizers on Bacterial Community Structure, Ecological Network, and Key Species in Fluvo-Aquic Soil

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