干旱胁迫下陇中紫花苜蓿对外源精胺的生理响应

doi:10.3864/j.issn.0578-1752.2025.04.005

Abstract

Abstract:

【Objective】Spermine (Spm) can improve the tolerance of plants to abiotic stresses and reduce the damage caused by abiotic stresses to plants. To investigate the effect of exogenous Spm on drought resistance of alfalfa under drought stress, to provide a theoretical basis for exogenous Spm to improve drought resistance of alfalfa, and then to provide a scientific basis for the application of utilizing exogenous Spm to enhance drought resistance of plants. 【Method】Longzhong alfalfa (Medicago sativa L. cv. Longzhong) was used as the experimental material, and polyethylene glycol 6000 was used to simulate drought stress. Under normal water and drought stress after the root application of 0.1, 0.5 and 1.0 mmol·L^-1 concentration of Spm, the change characteristics in Longzhong alfalfa were studied, including plant height, leaf relative water content (RWC), photosynthetic pigments content, gas exchange parameters, antioxidant enzymes activity, antioxidants content, superoxide anion ($O^{\bar{.}}_{2}$) content, hydrogen peroxide (H₂O₂) content, malondialdehyde (MDA) content and osmoregulatory substances content. 【Result】Under drought stress, exogenous Spm increased plant height and leaf RWC of Longzhong alfalfa, slowed down the degradation of chlorophyll a, chlorophyll b (Chlb), and carotenoids, elevated the net photosynthetic rate, transpiration rate, and stomatal conductance of leaves, reduced the concentration of intercellular carbon dioxide, enhanced the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), raised the ascorbic acid (ASA) content, ASA/ dehydroascobic acid (DHA) ratio, glutathione (GSH) content and GSH/ glutathione disulfide (GSSG) ratio, decreased DHA and GSSG content, enhanced ascorbate peroxidase and glutathione reductase activities, decreased $O^{\bar{.}}_{2}$ and H₂O₂ production, inhibited MDA accumulation, and increased proline and soluble sugar content. The results of principal component analysis and calculation of average affiliation function values of all physiological indexes under each treatment showed that Spm application under normal water conditions had no effect on the growth of alfalfa, whereas Spm application under drought condition improved the drought tolerance of Longzhong alfalfa under drought stress, with the best enhancement effect of 0.5 mmol·L^-1 Spm. A further redundancy analysis showed that 0.5 mmol·L^-1 Spm enhanced the drought tolerance of Longzhong alfalfa mainly by slowing down the degradation of leaf Chlb, increasing the ASA-GSH cycle and CAT activity.【Conclusion】Exogenous Spm could reduce the degradation of photosynthetic pigments, alleviate the non-stomatal restriction of drought stress on alfalfa leaves, increase the activities of SOD, POD and CAT, promote the ASA-GSH cycle, increase the content of osmoregulatory substances, reduce the production of $O^{\bar{.}}_{2}$ and H₂O₂, and decrease the degree of membrane lipid peroxidation, thus increasing the plant height and leaf RWC of Longzhong alfalfa under drought stress, thereby enhancing its drought resistance. Exogenous application of 0.5 mmol·L^-1 Spm was an effective method to improve the drought resistance of alfalfa under drought stress, and it could be applied to improve the drought resistance of alfalfa in dry areas for practical production.

Key words: alfalfa, drought stress, exogenous spermine, drought tolerance, physiology

WANG WenJuan, SHI ShangLi, KANG WenJuan, DU YuanYuan, YIN Chen. The Physiological Response of Longzhong Alfalfa to Exogenous Spermine Under Drought Stress[J].Scientia Agricultura Sinica, 2025, 58(4): 676-691.

Figures/Tables 11

Table 1

Experimental treatments and methods"

处理Treatment	具体方法Concrete method
正常水分+0 Spm Normal water+ 0 Spm	未经Spm预处理的陇中紫花苜蓿在Hoagland营养液中培养 Longzhong alfalfa without Spm pretreatment was cultivated in Hoagland nutrient solution
正常水分+0.1 mmol·L^-1 Spm Normal water + 0.1 mmol·L^-1 Spm	经0.1 mmol·L^-1 Spm预处理的陇中紫花苜蓿在Hoagland营养液中培养 Longzhong alfalfa pretreated with 0.1 mmol·L^-1 Spm was cultivated in Hoagland nutrient solution
正常水分+0.5 mmol·L^-1 Spm Normal water + 0.5 mmol·L^-1 Spm	经0.5 mmol·L^-1 Spm预处理的陇中紫花苜蓿在Hoagland营养液中培养 Longzhong alfalfa pretreated with 0.5 mmol·L^-1 Spm was cultivated in Hoagland nutrient solution
正常水分+1.0 mmol·L^-1 Spm Normal water + 1.0 mmol·L^-1 Spm	经1.0 mmol·L^-1 Spm 预处理的陇中紫花苜蓿在Hoagland营养液中培养 Longzhong alfalfa pretreated with 1.0 mmol·L^-1 Spm was cultivated in Hoagland nutrient solution
干旱+0 Spm Drought+ 0 Spm	未经Spm预处理的陇中紫花苜蓿在含有-1.2 MPa PEG-6000 的营养液中培养 Longzhong alfalfa without Spm pretreatment was cultivated in nutrient solution containing -1.2 MPa PEG-6000
干旱+0.1 mmol·L^-1 Spm Drought+ 0.1 mmol·L^-1 Spm	经0.1 mmol·L^-1 Spm预处理的陇中紫花苜蓿幼苗在含有-1.2 MPa PEG-6000 的营养液中培养 Longzhong alfalfa pretreated with 0.1 mmol·L^-1 Spm was cultivated in nutrient solution containing -1.2 MPa PEG-6000
干旱+0.5 mmol·L^-1 Spm Drought+ 0.5 mmol·L^-1 Spm	经0.5 mmol·L^-1 Spm预处理的陇中紫花苜蓿幼苗在含有-1.2 MPa PEG-6000的营养液中培养 Longzhong alfalfa pretreated with 0.5 mmol·L^-1 Spm was cultivated in nutrient solution containing -1.2 MPa PEG-6000
干旱+1.0 mmol·L^-1 Spm Drought+ 1.0 mmol·L^-1 Spm	经1.0 mmol·L^-1 Spm预处理的陇中紫花苜蓿幼苗在含有-1.2 MPa PEG-6000的营养液中培养 Longzhong alfalfa pretreated with 1.0 mmol·L^-1 Spm was cultivated in nutrient solution containing -1.2 MPa PEG-6000

Table 1

Fig. 1

Fig. 2

Fig. 3

Fig. 4

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Fig. 7

Table 2

Fig. 8

Fig. 9

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处理 Treatment	平均隶属函数值 Average affiliation function values	隶属函数排名 Ranking of affiliation functions
正常水分+0 Spm Normal water +0 Spm	0.49	4
正常水分+0.1 mmol·L^-1 Spm Normal water +0.1 mmol·L^-1 Spm	0.52	3
正常水分+0.5 mmol·L^-1 Spm Normal water +0.5 mmol·L^-1 Spm	0.52	3
正常水分+1.0 mmol·L^-1 Spm Normal water +1.0 mmol·L^-1 Spm	0.52	3
干旱+0 Spm Drought +0 Spm	0.24	6
干旱+0.1 mmol·L^-1 Spm Drought +0.1 mmol·L^-1 Spm	0.47	5
干旱+0.5 mmol·L^-1 Spm Drought +0.5 mmol·L^-1 Spm	0.80	1
干旱+1.0 mmol·L^-1 Spm Drought +1.0 mmol·L^-1 Spm	0.61	2

The Physiological Response of Longzhong Alfalfa to Exogenous Spermine Under Drought Stress

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