Scientia Agricultura Sinica ›› 2024, Vol. 57 ›› Issue (24): 4871-4883.doi: 10.3864/j.issn.0578-1752.2024.24.004

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Physiological Mechanism of Aluminum Tolerance of Rice Root Border Cells and Root Tips Induced by Nano Silica Biomineralization Deposition

FENG YingMing1,2(), NONG Wei1,3, CHEN XingYun1,4, HAN HongXiang1, ZHENG YuXin1, TIAN Xiao1, TANG Jiao1, GUO YiWei1, HUANG ChaoZheng1, LI XueWen1, SHI Lei2, YU Min1()   

  1. 1 Department of Horticulture, Foshan University/International Membrane Biology and Environment Research Center, Foshan 528000, Guangdong, China
    2 College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China
    3 Guangdong University of Business and Technology, Zhaoqing 526040, Guangdong, China
    4 School of Biological Science, University of Western Australia, Crawley 6009, Australia
  • Received:2024-06-05 Accepted:2024-09-02 Online:2024-12-16 Published:2024-12-23
  • Contact: YU Min

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Abstract:

【Objective】This study aimed to explore the physiological mechanism of silicon alleviating aluminum toxicity in plants, to study the effect of biomineralization structure constructed on rice root border cells and root tips on aluminum stress, so as to provide the theoretical and practical guidance for acid soil mineralization to alleviate aluminum toxicity of plants in southern China. 【Method】Employing rice (Oryza.Sativa L.) as the experimental materials, using root tips and root border cells as the research object, under 100 μmol·L-1 aluminum stress treatment, polyethylenimine induced nano silica to form biomineralization structure on the surface of root tips and root border cells. Four treatments are administered: bare cells without aluminum stress (-Si-Al), bare cells with aluminum stress (-Si+Al), silica-coated cells without aluminum stress (+Si-Al), and silica-coated cells with aluminum stress (+Si+Al). The study examined the cell viability, levels of active oxygen species, and localization of active aluminum in root border cells, as well as the relative elongation of the root tips, levels of active oxygen species, callose content and localization of active aluminum in the root tips. 【Result】Under aluminum stress, compared with non biomineralization, polyethylenimine induced nano silica deposition on the cell wall of root border cells, so the survival rate of root border cells increased by 21.04%, the level of reactive oxygen species decreased by 87.65%, and the relative fluorescence value increased by 77.09% after Morin staining, and then effectively improved cell survival rate, reduced ROS production, and slowed down the programmed cell death; after polyethylenimine induced nano silica deposition in root tip, the relative growth rate of root tip increased by 26.95%, the level of reactive oxygen species decreased by 27.73%, the content of callose increased by 55.29%, and the relative fluorescence value increased by 55.45% after Morin staining, hematoxylin staining also showed that more Al3+ was deposited in the meristematic and transitional zones of root tip, and this indicated that the biomineralization deposition could adsorb more Al3+ on the surface of root tip, prevent Al3+ from entering the root tip to protect, and then alleviate the toxic effect of aluminum on root tip. 【Conclusion】Polyethylenimine induced nano silica deposition on the cell wall endows rice root border cells and root tips with aluminum tolerance, and reduced aluminum accumulation in rice, thus ensuring food safety and human health.

Key words: rice, polyethyleneimine, nano silica, aluminum toxicity, apical, root border cells

Fig. 1

Effect of nano silica deposition on the survival rate of RBCs under aluminum stress 结果取3次独立测量的平均值（mean±SD，n=3），不同小写字母表示处理间存在显著性差异（P<0.05） Values are mean±SD of three independent sets of experiments, and different lowercase letters indicate a significant difference at P<0.05。下同 The same as below"

Fig. 2

Effect of nano silica deposition under aluminum stress on the level of reactive oxygen species in RBCs"

Fig. 3

Effect of nano silica deposition under aluminum stress on the localization of active aluminum in RBCs"

Fig. 4

Effect of nano silica deposition on rice root tips growth under aluminum stress"

Fig. 5

Effect of nano silica deposition under aluminum stress on the metabolism of reactive oxygen species in rice root tips"

Fig. 6

Effect of nano silica deposition on callose content in root tip of rice under aluminum stress"

Fig. 7

Effect of nano silica deposition on active aluminum localization in rice root tips under aluminum stress"

Fig. 8

Hematoxylin staining of rice root tips after nano silica deposition under aluminum stress"

Fig. 9

Physiological mechanism of aluminum tolerance in RBCs and root tip induced by biomineralization deposition of nano silica"

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