紫花苜蓿MsCEP基因家族的鉴定及其调控根系生长发育功能的分析

doi:10.3864/j.issn.0578-1752.2024.24.002

Abstract

Abstract:

【Objective】CEP (C-terminal encoded peptides) is a gene that encodes hormone-like peptides secreted by roots and serves as a key regulator of plant root growth and development. To provide a molecular theoretical basis for further elucidating the function of MsCEP genes in root growth and development, members of the Medicago sativa MsCEP gene family, basic characteristics, expression differences in different tissues, and their roles in root growth were identified and analyzed. 【Method】Based on the genomic information of the alfalfa cultivar Xinjiang Daye, the MsCEP gene family members of alfalfa were accurately identified using local Blast analysis in TBtools and feature domain by referring to MtCEP family protein of Medicago truncatula sequence. The fundamental genetic and protein characteristics and the phylogenetic relationship of the MsCEP genes were analyzed by bioinformatics methods. The expression patterns of alfalfa MsCEP gene family members in various tissues were assessed using transcriptome data and real-time fluorescence quantitative PCR. The functional roles of mature MsCEP peptides in root growth and development were analyzed by exogenous application experiments. 【Result】A total of 35 MsCEP family members were identified in the genome of alfalfa Xinjiang Daye, and these genes are distributed across 18 chromosomes, lack introns, and all possess an N-terminal signal peptide and one or two conserved domains of the CEP family. The MsCEP members displayed predicted amino acid length ranging from 59 to 150, with molecular weights spanning 6.7 to 16.2 kDa, the isoelectric points varying from 5.80 to 10.41, instability indices ranging from 30.63 to 89.93, aliphatic indices ranging from 54.41 to 134.88, and the grand average of hydropathicity ranging from -1.110 to 0.377. Subcellular localization predictions indicated that the MsCEP protein predominantly localizes to the nucleus, plasma membrane, chloroplast, and Golgi apparatus. Cluster analysis delineated three distinct branches within the family, aligning with counterparts from Arabidopsis thaliana and Medicago truncatula. The largest branch encompassed 48 CEP members. Collinearity analysis highlighted a collinear relationship between the MsCEP genes in alfalfa and those in Arabidopsis thaliana and Medicago truncatula. Tissue expression analysis revealed that members of the MsCEP family exhibit distinct tissue-specific expression patterns, with higher expression levels in roots and lower or no expression detected in leaves. Among them, 22 members exhibited higher expression levels in roots compared to other tissues. The exogenous application of synthetic mature MsCEP2 peptide suppressed the growth of primary and lateral roots, reduced the number of lateral roots, and decreased the density of lateral roots. 【Conclusion】In conclusion, our investigation identified a total of 35 MsCEP members from the alfalfa 'Xinjiangdaye' genome database, which are revealed to be highly conserved. The MsCEP genes are primarily expressed in roots, and the exogenous application of synthetic mature MsCEP peptides can regulate root morphology, indicating that MsCEP peptides play important roles in root growth and development of alfalfa.

Key words: alfalfa, MsCEP gene family, CEP2, exogenous application of peptide, root growth and development

ZENG XiangCui, YANG YongNian, LI RuYue, JIANG XueQian, JIANG Xu, XU YanRan, LIU ZhongKuan, LONG RuiCai, KANG JunMei, YANG QingChuan, LI MingNa. Identification of Alfalfa (Medicago sativa) MsCEP Genes and Functional Analysis of Its Regulation in Root Growth and Development[J].Scientia Agricultura Sinica, 2024, 57(24): 4839-4853.

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Figures/Tables 9

Table 1

The physiochemical characteristics of 35 members in the alfalfa MsCEP gene family"

基因编号 Gene ID	基因名称 Gene name	氨基酸数目 No. of amino acids	等电点 pI	分子量 Molecular weight (Da)	不稳定指数 Instability index	脂肪系数 Aliphatic index	平均疏水指数 Grand average of hydropathicity	亚细胞定位 Subcellular localization
MS.gene24367.t1	MsCEP1	107	6.40	12077.73	37.90	81.96	-0.428	细胞核Nucleus
MS.gene044007.t1	MsCEP2	107	5.86	12066.66	43.06	83.83	-0.407	细胞核Nucleus
MS.gene67093.t1	MsCEP3	107	5.86	12066.66	43.06	83.83	-0.407	细胞核Nucleus
MS.gene062764.t1	MsCEP4	107	5.86	12066.66	43.06	83.83	-0.407	细胞核Nucleus
MS.gene63725.t1	MsCEP5	86	10.41	9234.91	36.16	134.88	0.377	叶绿体Chloroplast
MS.gene002621.t1	MsCEP6	102	9.75	11158.06	52.67	99.31	-0.079	高尔基体、细胞核 Golgi apparatus, Nucleus
MS.gene01607.t1	MsCEP7	102	9.75	11158.06	52.67	99.31	-0.079	高尔基体、细胞核 Golgi apparatus, Nucleus
MS.gene03109.t1	MsCEP8	102	9.75	11145.06	52.67	99.31	-0.052	细胞核Nucleus
MS.gene004299.t1	MsCEP9	102	9.75	11144.03	48.89	99.31	-0.079	细胞核Nucleus
MS.gene049393.t1	MsCEP10	90	5.80	9926.39	61.99	79.11	-0.162	叶绿体、细胞核Chloroplast, Nucleus
MS.gene06520.t1	MsCEP11	90	5.80	9926.39	61.99	79.11	-0.162	叶绿体、细胞核Chloroplast, Nucleus
MS.gene013422.t1	MsCEP12	90	5.80	9926.39	61.99	79.11	-0.162	叶绿体、细胞核Chloroplast, Nucleus
MS.gene27830.t1	MsCEP13	99	10.11	10829.94	54.09	105.25	0.193	叶绿体、高尔基体、细胞核 Chloroplast, Golgi apparatus, Nucleus
MS.gene058837.t1	MsCEP14	99	10.11	10829.94	54.09	105.25	0.193	叶绿体、高尔基体、细胞核 Chloroplast, Golgi apparatus, Nucleus
MS.gene023603.t1	MsCEP15	99	10.11	10829.94	54.09	105.25	0.193	叶绿体、高尔基体、细胞核Chloroplast, Golgi apparatus, Nucleus
MS.gene28597.t1	MsCEP16	141	7.87	15729.38	41.08	60.14	-0.755	细胞核Nucleus
MS.gene28596.t1	MsCEP17	102	6.96	10651.16	50.09	98.43	0.108	细胞膜、细胞核Cell membrane, Nucleus
MS.gene44917.t1	MsCEP18	141	7.87	15743.4	42.15	60.14	-0.755	细胞核Nucleus
MS.gene44918.t1	MsCEP19	102	6.96	10651.16	50.09	98.43	0.108	细胞膜、细胞核Cell membrane, Nucleus
MS.gene44919.t1	MsCEP20	150	10.23	16286.74	31.10	68.80	-0.382	细胞膜Cell membrane
MS.gene82945.t1	MsCEP21	63	8.23	6692.53	89.93	63.49	-0.606	细胞核Nucleus
MS.gene011842.t1	MsCEP22	141	7.87	15743.4	42.15	60.14	-0.755	细胞核Nucleus
MS.gene011843.t1	MsCEP23	102	6.96	10651.16	50.09	98.43	0.108	细胞膜、细胞核Cell membrane, Nucleus
MS.gene011844.t1	MsCEP24	150	10.23	16286.74	31.10	68.8	-0.382	细胞膜Cell membrane
MS.gene056566.t1	MsCEP25	63	8.23	6722.56	84.82	61.90	-0.646	细胞核Nucleus
MS.gene80019.t1	MsCEP26	90	5.86	9511.51	52.70	70.44	-0.607	细胞核Nucleus
MS.gene80020.t1	MsCEP27	141	7.87	15743.4	42.15	60.14	-0.755	细胞核Nucleus
MS.gene80021.t1	MsCEP28	122	9.69	13361.94	30.63	65.33	-0.517	细胞核Nucleus
MS.gene80022.t1	MsCEP29	150	10.19	16238.65	31.23	68.80	-0.397	细胞膜Cell membrane
MS.gene067991.t1	MsCEP30	63	8.23	6722.56	84.82	61.90	-0.646	细胞核Nucleus
MS.gene28559.t1	MsCEP31	59	10.04	6739.56	72.42	54.41	-1.110	细胞核Nucleus
MS.gene28558.t1	MsCEP32	150	10.23	16286.74	31.10	68.80	-0.382	细胞膜Cell membrane
MS.gene28557.t1	MsCEP33	102	6.96	10651.16	50.09	98.43	0.108	细胞膜、细胞核Cell membrane, Nucleus
MS.gene28556.t1	MsCEP34	141	7.06	15716.33	39.26	58.79	-0.772	细胞核Nucleus
MS.gene83398.t1	MsCEP35	63	8.23	6722.56	84.82	61.90	-0.646	细胞核Nucleus

Table 1

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

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