茉莉酸调控马铃薯离体块茎发育的主要代谢物变化

doi:10.3864/j.issn.0578-1752.2024.13.003

Abstract

Abstract:

【Objective】 Jasmonic acid (JA) is one of key regulatory hormones during potato tuber development. Study on JA regulating tuber development mechanism would provide an important theoretical basis for tuber yield and quality formation.【Method】 In this study, potato stolons cultured in vitro were exogenously applied with JA (0, 0.5, 5, 50 μmol·L^-1) to analyze the phenotype, tissue microstructure, carbohydrate accumulation and proteome change of tubers.【Result】 With the increase of JA concentration, the tuber number formed per stolon, tuber diameter, fresh and dry weight, cell area of perimedullary zone, starch and soluble sugar content were gradually increased at 0.5 and 5 μmol·L^-1 JA (P<0.05), whereas the tuber diameter, fresh and dry weight, starch content were significantly decreased at 50 μmol·L^-1 JA (P<0.05). Lipoxygenase activity was gradually decreased with JA concentration increasing (P<0.05). Thirty-five differentially abundant proteins (P<0.05 and fold change≥2.5) closely related to tuber development regulating by JA were identified by 2-DE and MALDI-TOF/TOF-MS. They were mainly involved in bioenergy and metabolism (28.6%), cell defense and rescue (28.6%), protein biogenesis and storage (11.4%), signaling (8.6%), transcription (8.6%), unknown (8.6%) and miscellaneous (5.6%). The differential expression patterns of these proteins were clustered into three categories by hierarchical clustering analysis. Cluster 1 included 17 proteins that down-regulated at 0.5 μmol·L^-1 JA and up-regulated at 5 μmol·L^-1 JA, which mainly involved in bioenergy and metabolism, protein biogenesis and storage, signaling and transcription. Cluster 2 included 10 proteins that gradually up-regulated with JA concentration increasing, which were mainly involved in cell defense and rescue, bioenergy and metabolism, transcription. Cluster 3 included 8 proteins that down-regulated after JA treatments, which were mainly involved in bioenergy and metabolism, cell defense and rescue, protein biogenesis and storage. 【Conclusion】 Low JA concentration (0.5, 5 μmol·L^-1) might promote tuber morphogenesis by inducing cell enlargement in tuber perimedullary zone, intracellular sucrose and polysaccharide accumulation, and cell defense ability, whereas high JA concentration (50 μmol·L^-1) showed inhibitory effects.

Key words: Solanum tuberosum, jasmonic acid, proteome, tuber development, lipoxygenase, mass spectrum

LIANG LiJuan, CHENG LiXiang, YUAN JianLong, SA Gang, ZHANG Feng. Jasmonic Acid Regulates the Changes of Major Metabolites in Potato Tuber Development in vitro[J].Scientia Agricultura Sinica, 2024, 57(13): 2525-2538.

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Figures/Tables 9

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Fig. 2

Fig. 3

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Fig. 5

Table 1

Identification results of differentially abundant proteins during potato tuber development regulated by JA in vitro"

点序号^a Spot No.	蛋白名称 Ptotein name	物种 Organism	gi号^b gi No.	理论等电点/相对分子质量^c Theoretical pI/Mr (kDa)	得分^d Score	匹配肽段^e PN
生物能量与代谢相关蛋白质Bioenergy and metabolism-related proteins
6606	3-磷酸甘油醛脱氢酶 Glyceraldehyde 3-phosphatedehydrogenase	Solanum tuberosum	gi\|22094840	6.34/36.79	882	17
4805	烯醇化酶 Enolase	Solanum lycopersicum	gi\|350538295	5.68/48.05	1120	23
3819	烯醇化酶 Enolase	Solanum lycopersicum	gi\|350538295	5.68/48.05	1070	15
7610	假定的线粒体NAD依赖的苹果酸脱氢酶 Putative mitochondrial NAD-dependent malate dehydrogenase	Solanum tuberosum	gi\|21388544	8.87/36.40	1000	15
1409	尿苷二磷酸葡萄糖醛基转移酶1-6 UDP-glucuronosyltransferase1-6	Medicago truncatula	gi\|357512853	6.20/54.68	45	7
7701	甲酸脱氢酶 Formate dehydrogenase	Solanum lycopersicum	gi\|350538487	6.87/42.35	864	19
0804	类3-酮脂酰-CoA合酶17 3-ketoacyl-CoA synthase 17-like	Vitis vinifera	gi\|359479575	8.24/52.53	59	10
1309	双半乳糖二酰基甘油合酶2 Digalactosyldiacylglycerol synthase 2	Brachypodium distachyon	gi\|357112938	8.65/53.57	40	8
7311	类细胞色素P450 86A1 Cytochrome P450 86A1-like	Glycine max	gi\|356553060	9.30/59.44	41	4
1804	细胞色素P450 Cytochrome P450	Arabidopsis thaliana	gi\|19698839	8.92/58.80	58	12
细胞防御相关蛋白质Cell defense and rescue-related proteins
1715	铜锌超氧化物歧化酶 Cu/Zn-superoxide dismutase	Solanum tuberosum	gi\|13751866	5.28/14.56	553	8
8711	过氧化物酶 Peroxidase	Nicotiana tabacum	gi\|14031049	5.99/39.49	440	12
5306	脱氢抗坏血酸还原酶 Dehydroascorbate reductase	Solanum tuberosum	gi\|160347100	6.09/23.61	653	12
6827	多酚氧化酶 Polyphenol oxidase	Solanum tuberosum	gi\|1146426	7.18/68.10	492	19
5822	多酚氧化酶 Polyphenol oxidase	Solanum tuberosum	gi\|1146426	7.18/68.10	379	15
5501	I型几丁质酶 Class I chitinase	Solanum tuberosum	gi\|6707113	5.66/36.33	494	6
7505	内切几丁质酶2 Endochitinase 2	Solanum tuberosum	gi\|1705808	5.94/34.57	685	8
8307	假定的无毒性响应蛋白 Putative avirulence-responsive protein	Arabidopsis thaliana	gi\|15217702	8.23/29.08	49	8
2302	核苷酸结合位点富含亮氨酸重复序列抗病蛋白Nucleotide binding site-leucine-rich repeat disease resistance protein	Pyrus communis	gi\|40644864	5.03/5.80	46	4
7112	羧酸酯酶 Carboxylesterase	Malus pumila	gi\|82697935	6.02/34.62	35	2
转录相关蛋白质Transcription-related proteins
6714	核RNA结合蛋白 Nuclear RNA binding protein	Solanum tuberosum	gi\|257215078	6.47/39.06	590	14
0102	假定的富含甘氨酸RNA结合蛋白 Putative glycine-rich RNA binding protein-like	Solanum tuberosum	gi\|82623423	5.58/17.56	599	12
3310	成熟酶K Maturase K	Ledermanniella pusilla	gi\|327204748	9.70/37.54	45	7
蛋白质生物合成与贮藏相关蛋白质Protein biogenesis and storage-related proteins
8001	蛋白酶抑制剂Ⅱ前体类型B Proteinase inhibitor Ⅱ precursor type B	Solanum tuberosum	gi\|334898813	5.69/17.60	447	10
9703	类延伸因子1α蛋白 Elongation factor 1-alpha-like protein	Solanum tuberosum	gi\|77999257	9.20/44.33	303	7
0009	类天冬氨酸蛋白酶前体 Aspartic protease precursor-like	Solanum tuberosum	gi\|82623417	5.85/55.71	420	8
1907	热激蛋白70 Heat shock 70 protein	Spinacia oleracea	gi\|2654208	5.19/76.26	885	18
信号转导相关蛋白质Signaling-related proteins
8108	丝裂原活化蛋白激酶 MAP kinase	Medicago truncatula	gi\|357437463	5.43/103.11	62	14
6416	含EF-手型结构域的钙结合蛋白 Calcium-binding EF-hand-containing protein	Arabidopsis thaliana	gi\|18407118	4.34/15.32	52	7
7320	类磷脂酰肌醇-4-磷酸5-激酶1 Phosphatidylinositol-4-phosphate 5-kinase 1-like	Brachypodium distachyon	gi\|357117945	8.74/81.57	46	11
混杂Miscellaneous
8203	果实成熟蛋白 Fruit-ripening protein	Solanum lycopersicum	gi\|350539427	6.48/12.54	927	13
1507	Ran结合蛋白-1 Ran binding protein-1	Solanum lycopersicum	gi\|350538193	4.88/25.22	665	9
未知功能Unknown
2203	假定蛋白OsJ_24584 Hypothetical protein OsJ_24584	Oryza sativa Japonica Group	gi\|222637200	8.24/69.08	60	10
2401	Os09g0491852 Os09g0491852	Oryza sativa Japonica Group	gi\|297727117	4.96/39.15	51	5
6310	未知Unknown	Picea sitchensis	gi\|294461039	6.51/8.11	45	3

Table 1

Fig. 6

Fig. 7

Fig. 8

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