N-乙酰半胱氨酸对双酚A诱导的猪肾细胞凋亡和炎症反应的抑制作用

doi:10.3864/j.issn.0578-1752.2023.03.012

Abstract

Abstract:

【Background】 Bisphenol A (BPA) is widely used in the industrial manufacturing of plastic materials, which seeps out from plastic products and exposes to various environmental media such as food, water, soil, and air, resulting in long-term exposure of animals. It is passed to offspring through the placenta and breast milk, interfering with animal growth and development and adversely affecting animal growth performance and production efficiency. N-acetylcysteine (NAC), as a recognized potent antioxidant, can regulate various pathophysiological processes, such as oxidative stress, apoptosis, and inflammation. However, the regulatory role of NAC on BPA-induced porcine kidney cell injury remains unclear. 【Objective】This study aimed to explore the potential role of the antioxidant NAC on BPA-induced apoptosis and inflammatory responses in PK15 cells.【Method】 PK15 cells were selected as experimental materials, and the activities of catalase (CAT), total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) were measured by the corresponding antioxidant detection kits. PK15 cells were treated with different concentrations of NAC (0, 2, 5, 10 mmol·L^-1) and then co-treated with BPA, and then the cell viability was detected by CCK-8 to select the optimal concentration of NAC. The expression of apoptosis-related genes (BAX, BCL-2 and Caspase3) and inflammatory genes (IL-8, IL-6, IL-1β and TNF-α) as well as protein expression were detected by real-time quantitative PCR (qRT-PCR) and western blotting. The number of apoptotic cells and nuclear factor kappa B (NF-κB) nuclear translocation were detected by immunofluorescence staining. 【Result】 The results showed that BPA significantly reduced the activities of CAT, T-SOD and GSH-Px in PK15 cells, compared with the control group (P<0.05). CCK-8 results showed that BPA significantly decreased PK15 cell viability in contrast to control group, whereas the different concentrations of NAC significantly promoted cell viability, and 5, 10 mmol·L^-1 NAC pretreatment significantly promoted cell viability when compared with BPA alone. qRT-PCR and western blotting showed that BPA treatment significantly increased BAX and Caspase3 mRNA and protein expression, and decreased the BCL-2 mRNA and protein expression, whereas NAC pretreatment could reduce the increased BAX and Caspase3 expression and increase the decreased BCL-2 expression induced by BPA. Hoechst33258 fluorescence staining indicated that the cells treated with BPA showed strong blue fluorescence staining and obvious nuclear shrinkage, whereas NAC pretreated cell showed weak blue fluorescence. BPA treatment significantly increased the relative mRNA expression of inflammation-related factors (IL-8 and IL-6) (P<0.05), whereas NAC pretreatment inhibited BPA-induced increased inflammation-related factors (IL-8, IL-6 and IL-1β mRNA) relative expression. Immunofluorescence analysis of NF-κB nuclear translocation showed that NF-κB was mainly distributed in cytoplasm in the control group, whereas NF-κB was mainly distributed in the nucleus after BPA treatment and NAC pretreatment reduced nuclear NF-κB expression. 【Conclusion】 NAC significantly increased PK15 cell viability and inhibited PK15 cell apoptosis and inflammatory response induced by BPA.

Key words: Bisphenol A, N-acetylcysteine, apoptosis, inflammatory response, PK15 cell, oxidative stress

TAO WenJing, ZHANG ZiTing, LIU Yuan, SONG Dan, LI XiangChen. Inhibitory Effect of N-acetylcysteine on Bisphenol A-Induced Apoptosis and Inflammatory Response in Porcine Kidney Cells[J].Scientia Agricultura Sinica, 2023, 56(3): 549-558.

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引物名称 Primer name	引物序列 Primer sequence (5′-3′)	产物大小 Size (bp)
BAX	F: TTTGCTTCAGGGTTTCATCC R: GACACTCGCTCAACTTCTTGG	113
BCL-2	F: GCGACTTTGCCGAGATGT R: CACAATCCTCCCCCAGTTC	116
Caspase3	F: TTGGACTGTGGGATTGAGAC R: TTCGCCAGGAATAGTAACCAG	121
IL-6	F: CCCTGAGGCAAAAGGGAAAGA R: AGGAAATCCTCAAGGCTGCG	148
IL-8	F: AGAGTGGACCCCACTGTGAA R: TTGTTGTTGCTTCTCAGTTCTCT	137
IL-1β	F: GGAAGTGATGGCTAACTACGG R: CTGGATGCTCCCATTTCTCA	124
TNF-α	F: GCCCAAGGACTCAGATCATCG R: ATTGGCATACCCACTCTGCC	104
GAPDH	F: ACCCAGAAGACTGTGGATGG R: TTGAGCTCAGGGATGACCTT	207

Inhibitory Effect of N-acetylcysteine on Bisphenol A-Induced Apoptosis and Inflammatory Response in Porcine Kidney Cells

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