葡萄醇酰基转移酶编码基因遗传变异研究

doi:10.3864/j.issn.0578-1752.2022.14.010

Abstract

Abstract:

【Objective】The purpose of this study was to develop molecular markers suitable for the identification of grape aroma phenotypes, so as to provide a theoretical basis for grape molecular assisted breeding. 【Method】 The aroma components and content of 45 grape varieties were determined by solid-phase microextraction combined with gas-phase mass spectrometry, and the structural variation and SNP variation of VvAAT gene were analyzed by Sanger sequencing and amplicon sequencing, respectively. 【Result】A total of 65 aroma components were found in 45 grape varieties, which could be divided into four types: esters, alcohols, terpenes and aldehydes. Among them, the content of esters showed significant variety differences, and 20 grape varieties such as Kyoho were rich in volatile esters, but little volatile esters was detected in other 25 grape varieties such as 87-1. A total of 5 structural variants have been found in the VvAAT gene. Types I, II, IV and V could not be translated correctly due to premature termination codons or fragment insertion mutations. Only type III could be translated aright. According to the results of the phylogenetic tree analysis of their amino acid sequences, Type III could be divided into two types: III.1 and III.2. Combining the aroma data, it could be inferred that III.1 was functional, while the rest were non-functional. Amplicon sequencing and bioinformatics analysis revealed 8 SNP sites (T32C, A69T, G436C, A1247G, A1818T, G1929T, A1959G, and C1975G) located in the exon region of the VvAAT gene, all of which caused mutations in the coding amino acids. It could accurately distinguish between ester-rich varieties and ester-poor varieties, with an accuracy rate of 97.8%. 【Conclusion】VvAAT gene locus had abundant genetic variation, including gene structure variation and SNP variation; 8 SNP loci located in the coding region of VvAAT gene could accurately determine the phenotype of volatile esters, which could be applied to grape molecular assisted breeding.

Key words: grape, aroma, alcohol acyltransferase, VvAAT, amplicon sequencing, marker-assisted selection

JI XiaoHao,LIU FengZhi,WANG BaoLiang,LIU PeiPei,WANG HaiBo. Genetic Variation of Alcohol Acyltransferase Encoding Gene in Grape[J].Scientia Agricultura Sinica, 2022, 55(14): 2797-2811.

Figures/Tables 13

Table 1

Fig. 1

Table 2

Table 3

VvAAT genotype statistics of 27 grape varieties"

品种 Variety	基因名称 Gene name	NCBI注册号 GenBank accession number	基因结构类型 Gene structure type	表型 Phenotype
巨峰 Kyoho	VvAAT-Kyoho-1	OK559426	III.2	酯香型 Esters-rich
巨峰 Kyoho	VvAAT-Kyoho-2	OK559427	III.1	酯香型 Esters-rich
阳光玫瑰 Shine-Muscat	VvAAT-Shine_Musca-1	OK559428	IV	非酯香型 Esters-poor
阳光玫瑰 Shine-Muscat	VvAAT-Shine_Musca-2	OK559429	V	非酯香型 Esters-poor
着色香 Zhuosexiang	VvAAT-Zhuosexiang-1	OK559430	III.1	酯香型 Esters-rich
着色香 Zhuosexiang	VvAAT-Zhuosexiang-2	OK559431	V	酯香型 Esters-rich
87-1	VvAAT-87_1	OK559432	III.2	非酯香型 Esters-poor
晚17 Wan17	VvAAT-Wan17	OK559433	II	非酯香型 Esters-poor
8611	VvAAT-8611-1	OK559434	III.2	非酯香型 Esters-poor
	VvAAT-8611-2	OK559435	III.2	非酯香型 Esters-poor
香妃 Xiangfei	VvAAT-Xiangfei	OK559436	III.2	非酯香型 Esters-poor
京早晶 Jingzaojing	VvAAT-Jingzaojing	OK559437	III.2	非酯香型 Esters-poor
郑州早红Zhengzhouzaohong	VvAAT-Zhengzhouzaohong	OK559438	V	非酯香型 Esters-poor
索罗门 Madeleine_Salomon	VvAAT-Madeleine_Salomon-1	OK559439	III.2	非酯香型 Esters-poor
索罗门 Madeleine_Salomon	VvAAT-Madeleine_Salomon-2	OK559440	III.2	非酯香型 Esters-poor
京秀 Jingxiu	VvAAT-Jingxiu-1	OK559441	III.2	非酯香型 Esters-poor
京秀 Jingxiu	VvAAT-Jingxiu-2	OK559442	II	非酯香型 Esters-poor
早巨选 Zaojuxuan	VvAAT-Zaojuxuan-1	OK559443	III.2	酯香型 Esters-rich
	VvAAT-Zaojuxuan-2	OK559444	III.1
	VvAAT-Zaojuxuan-3	OK559445	III.2
	VvAAT-Zaojuxuan-4	OK559446	III.2
奥利文 Irsay_Oliver	VvAAT-Irsay_Oliver	OK559447	III.1	酯香型 Esters-rich
香槟 Champion	VvAAT-Champion	OK559448	V	非酯香型 Esters-poor
紫早 Zizao	VvAAT-Zizao	OK559449	III.1	酯香型 Esters-rich
里扎马特 Rizamat	VvAAT-Rizamat-1	OK559450	III.2	非酯香型 Esters-poor
里扎马特 Rizamat	VvAAT-Rizamat-2	OK559451	III.2	非酯香型 Esters-poor
228	VvAAT-228	OK559452	III.2	非酯香型 Esters-poor
安尼斯基 Ayaneskeal	VvAAT-Ayaneskeal	OK559453	III.1	酯香型 Esters-rich
阿登纳玫瑰 Muscat_Adda	VvAAT-Muscat_Adda	OK559454	II	非酯香型 Esters-poor
红富士 BenniFuji	VvAAT-BenniFuji	OK559455	III.1	酯香型 Esters-rich
吉香 Jixiang	VvAAT-Jixiang	OK559456	III.1	酯香型 Esters-rich
藤稔 Fujiminori	VvAAT-Fujiminori	OK559457	III.1	酯香型 Esters-rich
尼加拉 Nigara	VvAAT-Nigara-1	OK559458	III.2	酯香型 Esters-rich
尼加拉 Nigara	VvAAT-Nigara-2	OK559459	III.1	酯香型 Esters-rich
乌兹别克玫瑰 Ojin_Kokn	VvAAT-Ojin_Kokn	OK559460	III.2	非酯香型 Esters-poor
井川1014 Jingchun1014	VvAAT-Jingchun1014-1	OK559461	III.2	酯香型 Esters-rich
井川1014 Jingchun1014	VvAAT-Jingchun1014-2	OK559462	III.1	酯香型 Esters-rich
秦龙大穗 Qinlongdasui	VvAAT-Qinlongdasui-1	OK559463	III.1	酯香型 Esters-rich
秦龙大穗 Qinlongdasui	VvAAT-Qinlongdasui-2	OK559464	III.2	酯香型 Esters-rich
累克基特 Leikejite	VvAAT-Leikejite-1	OK559465	I	非酯香型 Esters-poor
累克基特 Leikejite	VvAAT-Leikejite-2	OK559466	II	非酯香型 Esters-poor

Table 3

Fig. 2

Fig. 3

Table 4

Table 5

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Table 6

References 40

[1]	穆维松, 冯建英, 田东, 牟鑫. 我国鲜食葡萄产业的国际贸易与国内需求形势. 中国果树, 2019(2): 5-10. doi: 10.16626/j.cnki.issn1000-8047.2019.02.002. doi: 10.16626/j.cnki.issn1000-8047.2019.02.002
	MU W S, FENG J Y, TIAN D, MU X. The international trade and domestic demand of the table grape industry in China. China Fruits, 2019(2): 5-10. doi: 10.16626/j.cnki.issn1000-8047.2019.02.002. (in Chinese) doi: 10.16626/j.cnki.issn1000-8047.2019.02.002
[2]	YANG C X, WANG Y J, LIANG Z C, FAN P G, WU B H, YANG L, WANG Y N, LI S H. Volatiles of grape berries evaluated at the germplasm level by headspace-SPME with GC-MS. Food Chemistry, 2009, 114: 1106-1114. doi: 10.1016/j.foodchem.2008.10.061
[3]	FENOLL J, MANSO A, HELLÍN P, RUIZ L, FLORES P. Changes in the aromatic composition of the Vitis vinifera grape Muscat Hamburg during ripening. Food Chemistry, 2009, 114: 420-428. doi: 10.1016/j.foodchem.2008.09.060
[4]	KALUA C M, BOSS P K. Evolution of volatile compounds during the development of Cabernet Sauvignon grapes (Vitis vinifera L.). Journal of Agricultural and Food Chemistry, 2009, 57: 3818-3830. doi: 10.1021/jf803471n
[5]	YANG C X, WANG Y J, WU B H, FANG J B, LI S H. Volatile compounds evolution of three table grapes with different flavour during and after maturation. Food Chemistry, 2011, 128: 823-830. doi: 10.1016/j.foodchem.2010.11.029
[6]	WANG D, CAI J, ZHU B Q, WU G F, DUAN C Q, CHEN G, SHI Y. Study of free and glycosidically bound volatile compounds in air-dried raisins from three seedless grape varieties using HS-SPME with GC-MS. Food Chemistry, 2015, 177: 346-353. doi: 10.1016/j.foodchem.2015.01.018. doi: 10.1016/j.foodchem.2015.01.018
[7]	WU Y S, DUAN S Y, ZHAO L P, GAO Z, LUO M, SONG S R, XU W P, ZHANG C X, MA C, WANG S P. Aroma characterization based on aromatic series analysis in table grapes. Scientific Reports, 2016, 6: 31116. doi: 10.1038/srep31116. doi: 10.1038/srep31116
[8]	AUBERT C, CHALOT G. Chemical composition, bioactive compounds, and volatiles of six table grape varieties (Vitis vinifera L.). Food Chemistry, 2018, 240: 524-533. doi: 10.1016/j.foodchem.2017.07.152. doi: 10.1016/j.foodchem.2017.07.152
[9]	李秋棉, 罗均, 李雪萍, 陈维信. 果实香气物质的合成与代谢研究进展. 广东农业科学, 2012, 39(19): 104-107. doi: 10.16768/j.issn.1004-874x.2012.19.061. doi: 10.16768/j.issn.1004-874x.2012.19.061
	LI Q M, LUO J, LI X P, CHEN W X. Advances in the study on the biosynthesis and metabolism of volatile compounds in fruits. Guangdong Agricultural Sciences, 2012, 39(19): 104-107. doi: 10.16768/j.issn.1004-874x.2012.19.061. (in Chinese) doi: 10.16768/j.issn.1004-874x.2012.19.061
[10]	SHALIT M, KATZIR N, TADMOR Y, LARKOV O, BURGER Y, SHALEKHET F, LASTOCHKIN E, RAVID U, AMAR O, EDELSTEIN M, KARCHI Z, LEWINSOHN E. Acetyl-coa: alcohol acetyltransferase activity and aroma formation in ripening melon fruits. Journal of Agricultural and Food Chemistry, 2001, 49(2): 794-799. doi: 10.1021/jf001075p. doi: 10.1021/jf001075p
[11]	SOULEYRE E J F, CHAGNÉ D, CHEN X Y, TOMES S, TURNER R M, WANG M Y, MADDUMAGE R, HUNT M B, WINZ R A, WIEDOW C, HAMIAUX C, GARDINER S E, ROWAN D D, ATKINSON R G. The AAT1 locus is critical for the biosynthesis of esters contributing to ‘ripe apple’ flavour in ‘Royal Gala’ and ‘Granny Smith’ apples. The Plant Journal, 2014, 78(6): 903-915. doi: 10.1111/tpj.12518. doi: 10.1111/tpj.12518
[12]	HARADA M, UEDA Y, IWATA T. Purification and some properties of alcohol acetyltransferase from banana fruit. Plant and Cell Physiology, 1985, 26(6): 1067-1074. doi: 10.1093/oxfordjournals.pcp.a077002. doi: 10.1093/oxfordjournals.pcp.a077002
[13]	PÉREZ A G, SANZ C, OLÍAS J M. Partial purification and some properties of alcohol acyltransferase from strawberry fruits. Journal of Agricultural and Food Chemistry, 1993, 41: 1462-1466. doi: 10.1021/jf00033a021
[14]	BEEKWILDER J, ALVAREZ-HUERTA M, NEEF E, VERSTAPPEN F W A, BOUWMEESTER H J, AHARONI A. Functional characterization of enzymes forming volatile esters from strawberry and banana. Plant Physiology, 2004, 135(4): 1865-1878. doi: 10.1104/pp.104.042580. doi: 10.1104/pp.104.042580
[15]	AHARONI A, KEIZER L C P, BOUWMEESTER H J, SUN Z K, ALVAREZ-HUERTA M, VERHOEVEN H A, BLAAS J, VAN HOUWELINGEN A M M L, DE VOS R C H, VAN DER VOET H, JANSEN R C, GUIS M, MOL J, DAVIS R W, SCHENA M, VAN TUNEN A J, O'CONNELL A P. Identification of the SAAT gene involved in strawberry flavor biogenesis by use of DNA microarrays. Plant Cell, 2000, 12: 647-661. doi: 10.1105/tpc.12.5.647
[16]	YAHYAOUI F E L, WONGS-AREE C, LATCHÉ A, HACKETT R, GRIERSON D, PECH J C. Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening. European Journal of Biochemistry, 2002, 269(9): 2359-2366. doi: 10.1046/j.1432-1033.2002.02892.x. doi: 10.1046/j.1432-1033.2002.02892.x
[17]	SOULEYRE E J F, GREENWOOD D R, FRIEL E N, KARUNAIRETNAM S, NEWCOMB R D. An alcohol acyl transferase from apple (cv. Royal Gala), MpAAT1, produces esters involved in apple fruit flavor. The FEBS Journal, 2005, 272(12): 3132-3144. doi: 10.1111/j.1742-4658.2005.04732.x. doi: 10.1111/j.1742-4658.2005.04732.x
[18]	BALBONTÍN C, GAETE-EASTMAN C, FUENTES L, FIGUEROA C R, HERRERA R, MANRIQUEZ D, LATCHÉ A, PECH J C, MOYA-LEÓN M A. VpAAT1, a gene encoding an alcohol acyltransferase, is involved in ester biosynthesis during ripening of mountain papaya fruit. Journal of Agricultural and Food Chemistry, 2010, 58(8): 5114-5121. doi: 10.1021/jf904296c. doi: 10.1021/jf904296c
[19]	GÜNTHER C S, CHERVIN C, MARSH K B, NEWCOMB R D, SOULEYRE E J F. Characterisation of two alcohol acyltransferases from kiwifruit (Actinidia spp.) reveals distinct substrate preferences. Phytochemistry, 2011, 72(8): 700-710. doi: 10.1016/j.phytochem.2011.02.026. doi: 10.1016/j.phytochem.2011.02.026
[20]	任雪岩, 刘光财, 李国鹏, 叶春海, 丰锋, 王俊宁. 乙烯利和1-MCP对菠萝蜜果实中AheAAT和AheERF表达的影响. 中国农业科学, 2019, 52(21): 3890-3902. doi: 10.3864/j.issn.0578-1752.2019.21.017. doi: 10.3864/j.issn.0578-1752.2019.21.017
	REN X Y, LIU G C, LI G P, YE C H, FENG F, WANG J N. Effects of ethephon and 1-MCP on the expression of AheAAT gene and AheERF transcription factors in jackfruit fruit. Scientia Agricultura Sinica, 2019, 52(21): 3890-3902. doi: 10.3864/j.issn.0578-1752.2019.21.017. (in Chinese) doi: 10.3864/j.issn.0578-1752.2019.21.017
[21]	LI D P, XU Y F, XU G M, GU L K, LI D Q, SHU H R. Molecular cloning and expression of a gene encoding alcohol acyltransferase (MdAAT2) from apple (cv. Golden Delicious). Phytochemistry, 2006, 67(7): 658-667. doi: 10.1016/j.phytochem.2006.01.027. doi: 10.1016/j.phytochem.2006.01.027
[22]	董静, 王桂霞, 钟传飞, 常琳琳, 孙健, 张宏力, 孙瑞, 石琨, 隗永青, 张运涛. 森林草莓醇酰基转移酶基因FvAATW2功能研究. 园艺学报, 2018, 45(1): 41-50. doi: 10.16420/j.issn.0513-353x.2017-0085. doi: 10.16420/j.issn.0513-353x.2017-0085
	DONG J, WANG G X, ZHONG C F, CHANG L L, SUN J, ZHANG H L, SUN R, SHI K, WEI Y Q, ZHANG Y T. Studying function of alcohol acyltransferase gene FvAATW2 of Fragaria vesca by over- expressing in tobacco and cultivated strawberry. Acta Horticulturae Sinica, 2018, 45(1): 41-50. doi: 10.16420/j.issn.0513-353x.2017-0085. (in Chinese) doi: 10.16420/j.issn.0513-353x.2017-0085
[23]	D'AURIA J C. Acyltransferases in plants: A good time to be BAHD. Current Opinion in Plant Biology, 2006, 9: 331-340. doi: 10.1016/j.pbi.2006.03.016
[24]	WANG J H, DE LUCA V. The biosynthesis and regulation of biosynthesis of Concord grape fruit esters, including ‘foxy’ methylanthranilate. The Plant Journal, 2005, 44(4): 606-619. doi: 10.1111/j.1365-313X.2005.02552.x. doi: 10.1111/j.1365-313X.2005.02552.x
[25]	XU X Q, CHENG G, DUAN L L, JIANG R, PAN Q H, DUAN C Q, WANG J. Effect of training systems on fatty acids and their derived volatiles in Cabernet Sauvignon grapes and wines of the north foot of Mt. Tianshan. Food Chemistry, 2015, 181: 198-206. doi: 10.1016/j.foodchem.2015.02.082. doi: 10.1016/j.foodchem.2015.02.082
[26]	JI X H, WANG B L, WANG X D, WANG X L, LIU F Z, WANG H B. Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapes. Journal of Integrative Agriculture, 2021, 20(6): 1525-1539. doi: 10.1016/S2095-3119(20)63481-5. doi: 10.1016/S2095-3119(20)63481-5
[27]	JI X H, WANG B L, WANG X D, SHI X B, LIU P P, LIU F Z, WANG H B. Effects of different color paper bags on aroma development of Kyoho grape berries. Journal of Integrative Agriculture, 2019, 18(1): 70-82. doi: 10.1016/S2095-3119(18)62008-8. doi: 10.1016/S2095-3119(18)62008-8
[28]	HU B, JIN J P, GUO A Y, ZHANG H, LUO J C AND GAO G. GSDS 2.0: An upgraded gene feature visualization server. Bioinformatics, 2015, 31(8): 1296-1297. doi: 10.1093/bioinformatics/btu817
[29]	CHEN S F, ZHOU Y Q, CHEN Y R, GU J. Fastp: An ultra-fast all-in-one FASTQ preprocessor. Bioinformatics, 2018, 34(17): 884-890. doi: 10.1093/bioinformatics/bty560. doi: 10.1093/bioinformatics/bty560
[30]	LI H, DURBIN R. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics, 2009, 25(14): 1754-1760. doi: 10.1093/bioinformatics/btp324. doi: 10.1093/bioinformatics/btp324
[31]	DANECEK P, BONFIELD J K, LIDDLE J, MARSHALL J, OHAN V, POLLARD M O, WHITWHAM A, KEANE T, MCCARTHY S A, DAVIES R M, LI H. Twelve years of SAMtools and BCFtools. GigaScience, 2021, 10(2): giab008. doi: 10.1093/gigascience/giab008. doi: 10.1093/gigascience/giab008
[32]	ROBINSON J T, THORVALDSDÓTTIR H, WINCKLER W, GUTTMAN M, LANDER E S, GETZ G, MESIROV J P. Integrative genomics viewer. Nature Biotechnology, 2011, 29(1): 24-26. doi: 10.1038/nbt.1754. doi: 10.1038/nbt.1754
[33]	MCKENNA A, HANNA M, BANKS E, SIVACHENKO A, CIBULSKIS K, KERNYTSKY A, GARIMELLA K, ALTSHULER D, GABRIEL S, DALY M, DEPRISTO M A. The genome analysis toolkiT: A MapReduce framework for analyzing next-generation DNA sequencing data. Genome Research, 2010, 20(9): 1297-1303. doi: 10.1101/gr.107524.110. doi: 10.1101/gr.107524.110
[34]	KNAUS B J, GRÜNWALD N J. Vcfr: a package to manipulate and visualize variant call format data in R. Molecular Ecology Resources, 2017, 17(1): 44-53. doi: 10.1111/1755-0998.12549. doi: 10.1111/1755-0998.12549
[35]	PINO J A, MESA J. Contribution of volatile compounds to mango (Mangifera indica L.) aroma. Flavour and Fragrance Journal, 2006, 21: 207-213. doi: 10.1002/ffj.1703
[36]	YANG Y Z, CUENCA J, WANG N, LIANG Z C, SUN H H, GUTIERREZ B, XI X J, ARRO J, WANG Y, FAN P G, LONDO J, COUSINS P, LI S H, FEI Z J, ZHONG G Y. A key ‘foxy’ aroma gene is regulated by homology-induced promoter indels in the iconic juice grape ‘Concord’. Horticulture Research, 2020, 7: 67-78. doi: 10.1038/s41438-020-0304-6
[37]	ESLING P, LEJZEROWICZ F, PAWLOWSKI J. Accurate multiplexing and filtering for high-throughput amplicon-sequencing. Nucleic Acids Research, 2015, 43(5): 2513-2524. doi: 10.1093/nar/gkv107. doi: 10.1093/nar/gkv107
[38]	LUNDBERG D S, YOURSTONE S, MIECZKOWSKI P, JONES C D, DANGL J L. Practical innovations for high-throughput amplicon sequencing. Nature Methods, 2013, 10(10): 999-1002. doi: 10.1038/nmeth.2634. doi: 10.1038/nmeth.2634
[39]	TAKEDA M, SAKAI K, TERASHIMA M, KANEDA H, HAYASHI H, TANAKA K, OKAMOTO K, TAKAHAMA T, YOSHIDA T, IWASA T, SHIMIZU T, NONAGASE Y, KUDO K, TOMIDA S, MITSUDOMI T, SAIGO K, ITO A, NAKAGAWA K, NISHIO K. Clinical application of amplicon-based next-generation sequencing to therapeutic decision making in lung cancer. Annals of Oncology, 2015, 26(12): 2477-2482. doi: 10.1093/annonc/mdv475. doi: 10.1093/annonc/mdv475
[40]	GUO Z F, WANG H W, TAO J J, REN Y H, XU C, WU K S, ZOU C, ZHANG J N, XU Y B. Development of multiple SNP marker panels affordable to breeders through genotyping by target sequencing (GBTS) in maize. Molecular Breeding, 2019, 39(3): 37. doi: 10.1007/s11032-019-0940-4. doi: 10.1007/s11032-019-0940-4

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

化合物类型 Type of compound	种类 Number	变异范围 Variation range (ng∙g^-1)	平均值 Mean (ng∙g^-1)	方差 Variance
酯类Esters	22	0—3238.77	465.67	777.60
醇类Alcohols	2	0—164.84	12.58	31.91
醛类Aldehydes	12	642.80—5485.97	1890.40	1171.33
萜类Terpenoids	29	0—8548.37	471.41	1601.95
总计 Total	65	800.43—11387.75	2835.44	2381.58

酯香型品种 Varieties rich in volatile esters	非酯香型品种 Varieties poor in volatile esters
红香蕉 Hongxiangjiao (6, 244.27)	8611 (0, 0)
高砂 Gaosha (9, 278.59)	累克基特 Leikejite (0, 0)
秦龙大穗 Qinlongdasui (8, 220.90)	蓓蕾玫瑰 Muscat Bailey (0, 0)
黑蜜 Heimi (9, 199.58)	克瑞森无核 Crimson_Seedless (0, 0)
秋蜜 Qiumi (13, 402.83)	8612 (0, 0)
琥珀 Hupo (9, 330.06)	齐查卡普列 Qichakapulie (0, 0)
红丹 Hongdan (16, 920.17)	牧丹红 Mudanhong (0, 0)
火星无核 Mars_Seedless (9, 408.72)	红意大利Red_Italian (0, 0)
着色香 Zhuosexiang (6, 543.78)	玫瑰花 Meiguihua (0, 0)
黑虎香 Heihuxiang (14, 591.16)	泽玉 Zeyu (0, 0)
藤稔 Fujiminori (13, 701.42)	巧保2号 Qiaobao2 (0, 0)
红富士 BenniFuji (10, 723.95)	红巴拉多蒂 Hongbaladuodi (0, 0)
井川1014 Jingchun1014 (9, 800.74)	乌兹别克玫瑰 Ojin_Kokn (0, 0)
巨峰 Kyoho (15, 1453.39)	阿登纳玫瑰 Muscat_Adda (0, 0)
尼加拉 Nigara (13, 1697.20)	228 (0, 0)
奥利文 Irsay_Oliver (7, 1708.50)	里扎马特 Rizamat (0, 0)
安尼斯基 Ayaneskeal (9, 1920.01)	香槟 Champion (0, 0)
吉香 Jixiang (9, 2200.84)	京秀 Jingxiu (0, 0)
紫早 Zizao (9, 2370.34)	索罗门 Madeleine_Salomon (0, 0)
早巨选 Zaojuxuan (19, 3238.77)	郑州早红 Zhengzhouzaohong (0, 0)
	87-1 (0, 0)
	晚17 Wan17 (0, 0)
	香妃 Xiangfei (0, 0)
	京早晶 Jingzaojing (0, 0)
	阳光玫瑰 Shine_Musca (0, 0)

品种 Variety	Clean reads (K)	Clean bases (M)	Q20 (%)	Q30 (%)	覆盖度 Coverage (K)
奥利文 Irsay_Oliver	235.22	30.18	90.66	87.57	15.09
早巨选 Zaojuxuan	407.65	52.72	90.77	86.72	26.36
火星无核 Mars_Seedless	359.00	46.58	90.83	87.73	23.29
红香蕉 Hongxiangjiao	445.26	56.83	90.85	87.80	28.41
琥珀 Hupo	553.36	71.05	91.05	88.03	35.52
红富士 BenniFuji	373.19	47.93	90.47	87.29	23.96
秋蜜 Qiumi	295.73	37.96	90.76	87.69	18.98
巨峰 Kyoho	379.10	48.84	90.93	87.88	24.42
井川1014 Jingchun1014	518.11	66.35	90.89	87.84	33.17
黑虎香 Heihuxiang	729.92	95.28	90.87	87.79	47.64
黑蜜 Heimi	601.47	78.07	91.05	88.01	39.03
秦龙大穗 Qinlongdasui	693.37	90.00	90.50	87.34	45.00
红丹 Hongdan	338.81	42.87	90.80	87.78	21.43
高砂 Gaosha	343.88	43.87	91.00	88.01	21.93
红巴拉多蒂 Hongbaladuodi	521.69	67.30	91.20	88.17	33.65
巧保2号 Qiaobao2	355.88	45.40	91.04	88.00	22.70
泽玉 Zeyu	1187.71	155.61	90.83	87.68	77.80
玫瑰花 Meiguihua	1771.53	234.87	90.68	87.46	117.43
红意大利 Red Italian	627.85	79.59	89.91	86.61	39.79
牧丹红 Mudanhong	1348.14	173.77	89.67	86.65	86.88
8612	1342.69	173.45	89.86	86.51	86.72
克瑞森无核 Crimson Seedless	569.85	70.73	90.29	87.14	35.36

Genetic Variation of Alcohol Acyltransferase Encoding Gene in Grape

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 13

References 40

Related Articles 15

Metrics

Comments

Recommended 0

基因名称 Gene name	SNPs								基因结构类型 Gene structure type
基因名称 Gene name	32	69	438	1247	1818	1929	1959	1975	基因结构类型 Gene structure type
VvAAT-Kyoho-1	T	A	G	A	A	G	A	C	III.2
VvAAT-Kyoho-2	C	T	C	G	T	T	G	G	III.1
VvAAT-Shine_Musca-1	T	A	G	A	A	G	A	C	IV
VvAAT-Shine_Musca-2	T	A	G	A	A	G	A	C	V
VvAAT-Zhuosexiang-1	C	T	C	G	T	T	G	G	III.1
VvAAT-Zhuosexiang-2	T	A	G	A	A	G	A	C	V
VvAAT-87_1	T	A	G	A	A	G	A	C	III.2
VvAAT-Wan17	T	A	G	A	A	G	A	C	II
VvAAT-8611-1	T	A	G	A	A	G	A	C	III.2
VvAAT-8611-2	T	A	G	A	A	G	A	C	III.2
VvAAT-Xiangfei	T	A	G	A	A	G	A	C	III.2
VvAAT-Jingzaojing	T	A	G	A	A	G	A	C	III.2
VvAAT-Zhengzhouzaohong	T	A	G	A	A	G	A	C	V
VvAAT-Madeleine_Salomon-1	T	A	G	A	A	G	A	C	III.2
VvAAT-Madeleine_Salomon-2	T	A	G	A	A	G	A	C	III.2
VvAAT-Jingxiu-1	T	A	G	A	A	G	A	C	III.2
VvAAT-Jingxiu-2	T	A	G	A	A	G	A	C	II
VvAAT-Zaojuxuan-1	T	A	G	A	A	G	A	C	III.2
VvAAT-Zaojuxuan-2	C	T	C	G	T	T	G	G	III.1
VvAAT-Zaojuxuan-3	T	A	G	A	A	G	A	C	III.2
VvAAT-Zaojuxuan-4	T	A	G	G	T	T	G	G	III.2
VvAAT-Irsay_Oliver	C	T	C	G	T	T	G	G	III.1
VvAAT-Champion	T	A	G	A	A	G	A	C	V
VvAAT-Zizao	C	T	C	G	T	T	G	G	III.1
VvAAT-Rizamat-1	T	A	G	A	A	G	A	C	III.2
VvAAT-Rizamat-2	T	A	G	A	A	G	A	C	III.2
VvAAT-228	T	A	G	A	A	G	A	C	III.2
VvAAT-Ayaneskeal	C	T	C	G	T	T	G	G	III.1
VvAAT-Muscat_Adda	T	A	G	A	A	G	A	C	II
VvAAT-BenniFuji	C	T	C	G	T	T	G	G	III.1
VvAAT-Jixiang	C	T	C	G	T	T	G	G	III.1
VvAAT-Fujiminori	C	T	C	G	T	T	G	G	III.1
VvAAT-Nigara-1	T	A	G	A	A	G	A	C	III.2
VvAAT-Nigara-2	C	T	C	G	T	T	G	G	III.1
VvAAT-Ojin_Kokn	T	A	G	A	A	G	A	C	III.2
VvAAT-Jingchun1014-1	T	A	G	A	A	G	A	C	III.2
VvAAT-Jingchun1014-2	C	T	C	G	T	T	G	G	III.1
VvAAT-Qinlongdasui-1	C	T	C	G	T	T	G	G	III.1
VvAAT-Qinlongdasui-2	T	A	G	A	A	G	A	C	III.2
VvAAT-Leikejite-1	T	A	G	G	T	T	G	G	I
VvAAT-Leikejite-2	T	A	G	A	A	G	A	C	II

[1]	FENG WeiQing, NI YuanQian, FEI Teng, LI YouMei, XIE ZhaoSen. Differences in Vascular Bundle Morphological Structure, Distribution, and Water Transport Function in Grape Fruits of Different Shapes [J]. Scientia Agricultura Sinica, 2026, 59(1): 161-178.
[2]	WANG SiQi, ZOU LiRen, BAI RuiWen, YAN Ke, WANG SiYang, QI XiaoGuang, SHEN HaiLin, WEN JingHui. Screening of Key Genes Related to Gibberellic Acid Regulation of Rachis Hardening in Honey Grapes [J]. Scientia Agricultura Sinica, 2026, 59(1): 179-189.
[3]	TAN XiBei, LAN XuYing, LIU ChongHuai, FAN XiuCai, JIANG JianFu, SUN Lei, LI Peng, YU ShuXin, ZHANG Ying. Changes of Secondary Metabolites in Grapes with Different Resistance Levels in Response to White Rot Infection [J]. Scientia Agricultura Sinica, 2025, 58(9): 1767-1778.
[4]	TANG XueShen, DANG ShiZhuo, ZHOU Juan, LI JiaHao, LI MeiHua, HU Hao, ZHANG YaHong. Analysis of VvBES1-1 Involvement in Flower Bud Differentiation of Red Globe Grape Based on Red and Blue Light Regulation [J]. Scientia Agricultura Sinica, 2025, 58(8): 1650-1662.
[5]	YANG CaiLi, LI YongZhou, HE LiangLiang, SONG YinHua, ZHANG Peng, LIU ZhaoXian, LI PengHui, LIU SanJun. Genome-Wide Identification and Analysis of TPS Gene Family and Functional Verification of VvTPS4 in the Formation of Monoterpenes in Grape [J]. Scientia Agricultura Sinica, 2025, 58(7): 1397-1417.
[6]	GUO AoLin, LIN JunXuan, LAI GongTi, HE LiYuan, CHE JianMei, PAN Ruo, YANG FangXue, HUANG YuJi, CHEN GuiXin, LAI ChengChun. Effect of VdF3′5′H2 Overexpression on the Accumulation of Anthocyanin Composition in Spine Grape Cells [J]. Scientia Agricultura Sinica, 2025, 58(4): 802-818.
[7]	ZHANG XiangKun, LI JiaYing, QIAO RuMeng, HE JingLei, WANG Li, SHI XiaoXin, DU GuoQiang. Effects of GFabV Under Different Zn Levels on Photosynthetic Efficiency and Photosynthesis-Related Gene Expression of ‘Shine Muscat’ Grapevine [J]. Scientia Agricultura Sinica, 2025, 58(24): 5190-5200.
[8]	WANG Di, HAN ShouAn, ZHANG Wen, WANG Min, SHI HuiDong, ZHU XueHui, BAI ShiJian, LIU XuPeng, TIAN Jia, XIE Hui. Effects of Different Plant Growth Regulators on Fruit and Raisin Quality of Thompson Seedless Grapes [J]. Scientia Agricultura Sinica, 2025, 58(18): 3767-3782.
[9]	YU Huan, LIN Ling, GUO RongRong, CAO XiongJun, WANG Bo, FANG JingGui, XIE ShuYu, HUANG XiaoYun, HAN JiaYu, BAI XianJin. Investigation and Evaluation of Inflorescence Attachment and Quality of Grape Germplasm Resources in The Hot Zone Guangxi [J]. Scientia Agricultura Sinica, 2025, 58(17): 3503-3515.
[10]	WANG HuiLing, ZHANG YingYing, YAN AiLing, WANG XiaoYue, LIU ZhenHua, REN JianCheng, XU HaiYing, SUN Lei. Multi-Omics Analysis Reveals the Changes of Monoterpenes and Anthocyanins Accumulation During Veraison in Red Muscat-Type Grape [J]. Scientia Agricultura Sinica, 2025, 58(13): 2645-2662.
[11]	WANG RuiZhi, LI Tao, QIAN XiangLing, ZHANG Ya, YANG XiaoZhuo, LI GuangYan, WEI HaiYan, ZHANG HongCheng, LIU GuoDong. Effects of Nitrogen Panicle Fertilizer on Yield, Quality and Aroma of Southern Japonica Rice Nanjing 9108 [J]. Scientia Agricultura Sinica, 2025, 58(12): 2316-2332.
[12]	CAO XiongJun, WANG Bo, HAN JiaYu, LIAO YongFeng, XIE ShuYu, BAI Yang, HUANG XiaoYun, LU Li, HUANG QiuMi, JIANG ChunFen, PAN FengPing, BAI XianJin. Research and Practice on High Photosynthetic Efficiency Breeding of Grapes in Hot Climate Regions [J]. Scientia Agricultura Sinica, 2025, 58(10): 1994-2007.
[13]	DONG Jie, ZHANG Peng, LI WangZe, LI HeFang, ZHOU GuoChao, CHEN KeQin, FANG YuLin, ZHANG KeKun. Effects of Seedlessness and Swelling Treatments Based on GA₃ and CPPU on the Fruit Quality of 'Shine Muscat' Grapes [J]. Scientia Agricultura Sinica, 2025, 58(10): 2008-2021.
[14]	ZHANG SiNing, ZHANG XingRui, WU DongXuan, KANG JingBo, CHEN XiaoLin, GENG LiJun, YIN GuangMin, CHEN JiaJing, GAO JunYan, CAI ZhongHu, LIU Yuan, XU Juan. Aroma Quality Analysis of Guangdongxiangshui Lemon Based on Molecular Sensory Technology [J]. Scientia Agricultura Sinica, 2025, 58(1): 141-155.
[15]	GE Yi, ZHENG QiuLing, CHEN MengXia, XIA JiaXin, FANG Xiang, TANG MeiLing, FANG JingGui, SHANGGUAN LingFei. Cloning and Functional Analysis of the Autophagy Gene ATG8f in the Grapevine [J]. Scientia Agricultura Sinica, 2025, 58(1): 156-169.

	I	II	III.1	III.2	IV	V
I	0	-	-	-	-	-
II		0	-	0	-	-
III.1			1604.18	1482.20	-	543.78
III.2				0	-	-
IV					-	0
V						0