陆地棉遗传标准系TM-1的特性及其耐冷性

doi:10.3864/j.issn.0578-1752.2022.08.003

Abstract

Abstract:

【Objective】We systematically investigated the major agronomic traits and cold tolerance of accession TM-1 at the bud and seedling stages. The relative expressions of cold tolerance-related genes were analyzed by the qRT-PCR method. The cold tolerance mechanism of TM-1 was further discussed, which provides the theoretical basis for the breeding utilization of TM-1.【Method】The major agronomic traits of TM-1 were manually investigated in the field using variety CRI35 as the control. The fiber quality was assessed by an international calibrated cotton standard (HVICC), and the insect resistance (Bt) was detected by kanamycin screening and molecular detection technologies. For the cold tolerance testing, two contrasting accessions, cold-resistant accession Yu 2067 and cold-sensitive variety Hengmian 3 were set as controls, respectively. The cold resistance of TM-1 at bud stage and cotyledon stage was identified, treated at 4℃ and then recovered under normal conditions for 7 days, and the relative cotyledon spreading rate and the cold injury levels of plants were investigated, and cold injury indexes and cold resistance indexes were calculated. The portable chlorophyll meter was used for in vivo testing the leaf relative chlorophyll content (represented by SPAD value). The expressions of cold tolerance-related genes in leaves were measured by qRT-PCR method. 【Result】 The leaves of TM-1 were large and dark green. The pre-frost seed cotton yield was 2 791.50 kg·hm^-2, and the plant height was 94.60 cm. The growth period was about 135 days, and the yield, plant height, fruit branch number per plant, boll number per plant were higher than CRI35, while other agronomic traits were similar to CRI35. TM-1 had medium fiber quality. The test results of kanamycin and test paper showed that TM-1 did not contain the Bt like CRI35. Identification results of cold tolerance at bud stage showed that compared with the control treatment, the relative chlorophyll content and plant height of TM-1 decreased significantly. Low-temperature stress significantly inhibited hypocotyl elongation and chlorophyll synthesis in cotton leaves. Under low-temperature treatment, the taproots of TM-1 were damaged, but the lateral roots were more developed than those of the control. The cold tolerance level of TM-1 reached high cold resistance at the bud stage. Identification of cold tolerance at the cotyledon stage showed that the relative chlorophyll content and plant height of TM-1 decreased significantly compared with the control. The cold tolerance index of TM-1 at the cotyledon stage was 85.32%, which was significantly higher than Yu 2067, and the tolerance level of TM-1 reached cold resistance at the cotyledon stage. After the treatment of low-temperature stress for 24 h at the trefoil stage, nine genes were up-regulated in the TM-1 leaves, and their up-regulated expression folds were significantly higher than those of cold-sensitive accession. Dehydrin gene was up-regulated in TM-1 leaves, and the expression fold was similar to that in the leaves of Yu 2067, which was 4.69 times that in the leaves of Hengmian 3. The expression fold of the LEA3 gene in TM-1 leaves was significantly higher than that of Yu 2067 and Hengmian 3. 【Conclusion】 Accession TM-1 has stable agronomic characters and the medium fiber quality. It can be used as an ideal receptor for transferring exotic genes because without Bt. TM-1 can also be used as an important parent for cotton breeding and a gene source for cloning genes because of its good cold tolerance.

Key words: cotton, low temperature stress, TM-1, cold tolerance

WANG JunJuan, LU XuKe, WANG YanQin, WANG Shuai, YIN ZuJun, FU XiaoQiong, WANG DeLong, CHEN XiuGui, GUO LiXue, CHEN Chao, ZHAO LanJie, HAN YingChun, SUN LiangQing, HAN MingGe, ZHANG YueXin, FAN YaPeng, YE WuWei. Characteristics and Cold Tolerance of Upland Cotton Genetic Standard Line TM-1[J].Scientia Agricultura Sinica, 2022, 55(8): 1503-1517.

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References 36

[1]	KOHEL R J, LEWIS C F, RICHMOND T R. Texas marker-1: Description of a genetic standard for Gossypium hirsutum L.. Crop Science, 1970, 10(6): 670-671. doi: 10.2135/cropsci1970.0011183X001000060019x
[2]	GUO W Z, MA G J, ZHU Y C, YI C X, ZHANG T Z. Molecular tagging and mapping of quantitative trait loci for lint percentage and morphological marker genes in upland cotton. Journal of Integrative Plant Biology, 2006, 48(3): 320-326. doi: 10.1111/j.1744-7909.2006.00174.x
[3]	王鹏, 丁业掌, 陆琼娴, 郭旺珍, 张天真. 陆地棉遗传标准系TM-1背景的海岛棉染色体片段置换系的培育. 科学通报, 2008, 53(9): 1065-1069.
	WANG P, DING Y Z, LU Q X, GUO W Z, ZHANG T Z. Cultivation of Gossypium barbadense chromosome fragment replacement line with the background of upland cotton genetic standard line TM-1. Chinese Science Bulletin, 2008, 53(9): 1065-1069. (in Chinese)
[4]	付央, 苑冬冬, 胡文静, 蔡彩平, 郭旺珍. 陆地棉背景下海岛棉第18染色体片段置换系的培育及相关农艺性状QTL定位. 作物学报, 2013, 399(1): 21-28.
	FU Y, YUAN D D, HU W J, CAI C P, GUO W Z. Development of Gossypium barbadense chromosome 18 segment substitution lines in the genetic standard line TM -1 of Gossypium hirsutum and mapping of QTLs related to agronomic traits. Acta Agronomica Sinica, 2013, 399(1): 21-28. (in Chinese)
[5]	李青, 鱼海鹏, 张子豪, 孙正文, 张艳, 张冬梅, 王省芬, 马峙英, 阎媛媛. 棉花真叶原生质体分离及瞬时表达体系的优化. 中国农业科学, 2021, 54(21): 4514-4524.
	LI Q, YU H P, ZHANG Z H, SUN Z W, ZHANG Y, ZHANG D M, WANG S F, MA Z Y, YAN Y Y. Optimization of cotton mesophyll protoplast transient expression system. Scientia Agricultura Sinica, 2021, 54(21): 4514-4524. (in Chinese)
[6]	LI F G, FAN G Y, LU C R, XIAO G H, ZOU C S, KOHEL R I, MA Z Y, SHANG H H, MA X F, WU J Y, LIANG X M, HUANG G, PERCY R G, LIU K, YANG W H, CHEN W B, DU X M, SHI C C, YUAN Y L, YE W W, LIU X, ZHANG X Y, LIU W Q, WEI H L, WEI S J, HUANG G D, ZHANG X L, ZHU S J, ZHANG H, SUN F M, WANG X F, LIANG J, WANG J H, HE Q, HUANG L H, WANG J, CUI J J, SONG G L, WANG K B, XU X, YU J Z, ZHU Y X, YU S X. Genome sequence of cultivated upland cotton (Gossypium hirsutum TM-1) provides insights into genome evolution. Nature Biotechnology, 2015, 33(5): 524-530. doi: 10.1038/nbt.3208
[7]	赵晶, 李旭彤, 梁学忠, 王志城, 崔静, 陈斌, 吴立强, 王省芬, 张桂寅, 马峙英, 张艳. 陆地棉漆酶基因家族鉴定及在黄萎病菌胁迫下的表达分析. 作物学报, 2019(12): 1784-1795. doi: 10.3724/SP.J.1006.2019.94053
	ZHAO J, LI X T, LIANG X Z, WANG Z C, CUI J, CHEN B, WU L Q, WANG X F, ZHANG G Y, MA Z Y, ZHANG Y. Genome-wide identification of laccase gene family in update G. hirsutum L. genome and expression analysis under V. dahliae stress. Acta Agronomica Sinica, 2019(12): 1784-1795. (in Chinese) doi: 10.3724/SP.J.1006.2019.94053
[8]	YANG X M, LU X K, CHEN X G, WAGN D L, WANG J J, WANG S, GUO L X, CHEN C, WANG X G, WANG X L, YE W W. Genome-wide identification and expression analysis of DNA demethylase family in cotton. Journal of Cotton Research, 2019, 2(2): 142-150.
[9]	DOU L L, LV L M, KANG Y Y, TIAN R J, HUANG D Q, LI J Y, LI S Y, LIU F P, CAOL Y, JIN Y H, LIU Y, LI H Z, WANGW B, PANG C Y, SHANG H H, ZOU C S, SONG G L, XIAO G H. Genome-wide identification and expression analysis of the GhIQD gene family in upland cotton (Gossypium hirsutum L.). Journal of Cotton Research, 2021, 4(1): 1-14. doi: 10.1186/s42397-020-00077-x
[10]	王俊娟, 王帅, 陆许可, 阴祖军, 王德龙, 樊伟莉, 穆敏, 郭丽雪, 叶武威, 喻树迅. 棉花幼苗对低温胁迫的响应及抗冷机制初步研究. 棉花学报, 2017, 29(2): 34-43.
	WANG J J, WANG S, LU X K, YIN Z J, WANG D L, FAN W L, MU M, GUO L X, YE W W, YU S X. The effect of low temperature stress on the growth of upland cotton seedings and a preliminary study of cold-resistance of mechanisms. Cotton Science, 2017, 29(2): 34-43. (in Chinese)
[11]	李鹏程, 郑苍松, 孙淼, 冯卫娜, 邵晶晶, 朱海勇, 李亚兵, 董合林. 不同形态氮肥对棉花¹⁵N回收率和产量的影响. 中国土壤与肥料, 2021(5): 53-57.
	LI P C, ZHENG C S, SUN M, FENG W N, SHAO J J, ZHU H Y, LI Y B, DONG H L. Effects of different nitrogen forms on ¹⁵N recovery and yield of cotton. Soil and Fertilizer Sciences in China, 2021(5): 53-57. (in Chinese)
[12]	刘正德, 李运海, 罗云佳, 王红梅, 谭联望. 棉花新品种中棉所35的选育与应用技术. 中国棉花, 1999(11): 28-29.
	LIU Z D, LI Y H, LUO Y J, WANG H M, TAN L W. Breeding and application technology of a new cotton variety CRI35. China Cotton, 1999(11): 28-29. (in Chinese)
[13]	罗晓丽, 张安红, 肖娟丽, 王志安, 陈晓英, 吴家和. 棉蚜腺苷三磷酸酶E亚基基因RNAi载体的构建及其抗蚜性分析. 棉花学报, 2018, 30(5): 353-362.
	LUO X L, ZHANG A H, XIAO J L, WANG Z A, CHEN X Y, WU J H. Transgenic cotton expressing double-stranded RNAs of agATPase subunit E gene increases resistance to aphids. Cotton Science, 2018, 30(5): 353-362. (in Chinese)
[14]	王俊娟, 王德龙, 阴祖军, 王帅, 樊伟丽, 陆许可, 穆敏, 郭丽雪, 叶武威, 喻树迅. 陆地棉萌发至幼苗期抗冷性的鉴定. 中国农业科学, 2016, 49(17): 3332-3346.
	WANG J J, WANG D L, YIN Z J, WANG S, FAN W L, LU X K, MU M, GUO L X, YE W W, YU S X. Identification of the chilling resistance from germination stage to seedling stage in upland cotton. Scientia Agricultura Sinica, 2016, 49(17): 3332-3346. (in Chinese)
[15]	王俊娟. 棉花抗冷性鉴定及相关基因的表达研究[D]. 北京: 中国农业科学院, 2016.
	WANG J J. Identification of the chilling resistance of cotton and the expression of cold resistance related genes[D]. Beijing: Chinese Academy of Agricultural Sciences, 2016. (in Chinese)
[16]	段志坤, 秦晓惠, 朱晓红, 宋纯鹏. 解析植物冷信号转导途径: 植物如何感知低温. 植物学报, 2018, 53(2): 149-153. doi: 10.11983/CBB18039
	DUAN Z K, QIN X H, ZHU X H, SONG C P. Making sense of cold signaling: ICE is cold or not cold. Bulletin of Botany, 2018, 53(2): 149-153. (in Chinese) doi: 10.11983/CBB18039
[17]	ZHANG D J, GUO X Y, XU Y Y, LI H, MA L, YAO X F, WENG Y X, GUO Y, LIU C M, CHONG K. OsCIPK7point-mutation leads to conformation and kinase-activity change for sensing cold response. Journal of Integrative Plant Biology, 2019, 61(12): 1194-1200. doi: 10.1111/jipb.12800
[18]	KOBAYASHI M, HORIUCHI H, FUJITA K, TAKUHARA Y, SUZUKI S. Characterization of grape C-repeat-binding factor 2 and B-box-type zinc finger protein in transgenic Arabidopsis plants under stress conditions. Molecular Biology Reports, 2012, 39(8): 7933-7939. doi: 10.1007/s11033-012-1638-4
[19]	倪晓详, 王晓荣, 陆军, 从青, 程龙军. 巨桉锌指结构蛋白基因EgrZFP7在低温胁迫中的功能研究. 农业生物技术学报, 2018, 26(8): 1288-1295.
	NI X X, WANG X R, LU J, CONG Q, CHENG L J. Study on the function of zinc finger protein gent EgrZFP7 in cold stress response of Eucalyptus Grandis. Chinese Journal of Agricultural Biotechnology, 2018, 26(8): 1288-1295. (in Chinese)
[20]	XU Y, HU W, LIU J H, SONG S, HOU X W, JIA C H, LI J Y, MIAO H X, WANG Z, TIE W W, XU B Y, JIN Z Q.An aquaporin gene MaPIP2-7 is involved in tolerance to drought, cold and salt stresses in transgenic banana(Musa acuminata L.). Plant Physiology and Biochemistry, 2020, 147: 66-76. doi: 10.1016/j.plaphy.2019.12.011
[21]	ZHANG Y, YU H J, YANG X Y, LI Q, LING J, WANG H, GU X F, HUANG S W, JIANG W J. CsWRKY46, a WRKY transcription factor from cucumber, confers cold resistance in transgenic-plant by regulating a set of cold-stress responsive genes in an ABA-dependent manner. Plant Physiology and Biochemistry, 2016, 108: 478-487. doi: 10.1016/j.plaphy.2016.08.013
[22]	CHEN G Y, LI Y H, WEI Z Z, GAN L, LIU J S, WANG Z. Dynamic profiles of DNA methylation and the interaction with histone acetylation during fiber cell initiation of Gossypium hirsutum. Journal of Cotton Research, 2022, 5(8): 1-14. doi: 10.1186/s42397-021-00108-1
[23]	李俊兰, 崔淑芳, 金卫平, 黎鸿慧, 王广恩. 转Bt基因抗虫棉株卡那霉素抗性与抗虫性的关系研究. 河北农业科学, 2004(3): 112-113.
	LI J L, CUI S F, JIN W P, LI H H, WANG G E. Study on the relation between kanamycine resistance and boll worm resistance of transgenic cotton. Journal of Hebei Agricultural Sciences, 2004(3): 112-113. (in Chinese)
[24]	吴雪霞, 查丁石, 邰翔. 低温胁迫对茄子幼苗生长、抗氧化酶活性和渗透调节物质的影响. 江苏农业学报, 2008(4): 471-475.
	WU X X, ZHA D S, TAI X. Effects of low temperature stress on growth, activities of antioxidant enzymes and osmotic substance of eggplant seedlings. Jiangsu Journal of Agricultural Sciences, 2008(4): 471-475. (in Chinese)
[25]	尹晓斐. 低温胁迫对棉花生理特性的影响及关键酶基因表达分析[D]. 太原: 山西农业大学, 2013.
	YIN X F. Effect of low temperature stress on physiological characteristics in cotton (Gossypium spp.) and expression analysis of key enzymes genes[D]. Taiyuan: Shanxi Agricultural University, 2013. (in Chinese)
[26]	IVANOV A G, ROSSO D, SAVITCH L V, STACHULA P, ROSEMBERT M, OQUIST G, HURRY V, HVNER N P A. Implications of alternative electron sinks in increased resistance of PSII and PSI photochemistry to high light stress in cold-acclimated Arabidopsis thaliana. Photosynthesis Research, 2012, 113(1/3): 191-206. doi: 10.1007/s11120-012-9769-y
[27]	MU Q, LI X Y, LUO J H, PAN Q W, LI Y, GU T T. Characterization of expansin genes and their transcriptional regulation by histone modifications in strawberry. Planta, 2021, 254(2): 1-14. doi: 10.1007/s00425-021-03652-x
[28]	LI X X, ZHAO J, WALK T C, LIAO H. Characterization of soybean β-expansin genes and their expression responses to symbiosis, nutrient deficiency, and hormone treatment. Applied Microbiology & Biotechnology, 2014, 98(6): 2805-2817.
[29]	LIU W M, XU L A, LIN H, CAO J S. Two expansin genes, AtEXPA4 and AtEXPB5, are redundantly required for pollen tube growth and AtEXPA4is involved in primary root elongation in Arabidopsis thaliana. Genes, 2021, 12(2): 249. doi: 10.3390/genes12020249
[30]	刘栩铭, 李敏, 张曼, 霍红雁, 何智彪, 张继星, 王晓宇. 蓖麻3个PIP基因的克隆与冷胁迫下的表达分析. 农业生物技术学报, 2020, 28(10): 1788-1797.
	LIU X M, LI M, ZHANG M, HUO H Y, HE Z B, ZHANG J X, WANG X Y. Cloning of 3 PIPs from Ricinus communis and expression analysis under cold stresses. Chinese Journal of Agricultural Biotechnology, 2020, 28(10): 1788-1797. (in Chinese)
[31]	鲁雨晴, 崔亚宁, 张原, 姚小敏, 李晓娟. 植物水通道蛋白PIPs亚细胞定位转运的研究进展. 电子显微学报, 2020, 39(6): 779-786.
	LU Y Q, CUI Y N, ZHANG Y, YAO X M, LI X J. Advances in research on subcellular redistribution of plant plasma membrane aquaporin. Journal of Chinese Electron Microscopy Society, 2020, 39(6): 779-786. (in Chinese)
[32]	POU A, JEANGUENIN L, MILHIET T, BATOKO H, XHAUMONT F, HACHEZ C.Salinity-mediated transcriptional and post-translational regulation of the Arabidopsis aquaporin PIP2;7. Plant Molecular Biology, 2016, 92(6): 1-14. doi: 10.1007/s11103-016-0492-5
[33]	王俊娟, 穆敏, 王帅, 陆许可, 陈修贵, 王德龙, 樊伟丽, 阴祖军, 郭丽雪, 叶武威, 喻树迅. 棉花脱水素GhDHN1的克隆及其表达. 中国农业科学, 2016, 49(15): 2867-2878.
	WANG J J, MU M, WANG S, LU X K, CHEN X G, WANG D L, FAN W L, YIN Z J, GUO L X, YE W W, YU S X.Molecular clone and expression of GhDHN1 gene in cotton (Gossypium hirsutum L.). Scientia Agricultura Sinica, 2016, 49(15): 2867-2878. (in Chinese)
[34]	王俊娟, 陆许可, 阴祖军, 王德龙, 王帅, 穆敏, 陈修贵, 郭丽雪, 樊伟丽, 陈超, 叶武威. 陆地棉GhLEA3基因的克隆及其响应低温胁迫表达分析. 棉花学报, 2019, 31(2): 89-100.
	WANG J J, LU X K, YIN Z J, WANG D L, WANG S, MU M, CHEN X G, GUO L X, FAN W L, CHEN C, YE W W. Isolation and characterization of GhLEA3 gene from upland cotton and its expression in response to low temperature stress. Cotton Science, 2019, 31(2): 89-100. (in Chinese)
[35]	GEORGE S, USHA B, PARIDA A. Isolation and characterization of an atypical LEA protein coding cDNA and its promoter from drought-tolerant plant Prosopis juliflora. Applied Biochemistry and Biotechnology, 2009, 157(2): 244-253. doi: 10.1007/s12010-008-8398-6
[36]	ATREYEE K, SUBHARTHI D, SOURAV B, YASUFUMI K, YURIKO K, KOYAMA H, MARKKANDAN G. GhSTOP1, a C2H2 type zinc finger transcription factor is essential for aluminum and proton stress tolerance and lateral root initiation in cotton. Plant Biology, 2019, 21(1): 35-44. doi: 10.1111/plb.12895

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名称 Name	来源或培育单位 Origin or the breeding group	亲本组合 Parental combinations
TM-1	国家棉花种质中期库（安阳） National Medium-term Genebank of Cotton Germplasm Resource in China (Anyang)	品系D&PL-14 The line D&PL-14
豫2067 Yu 2067	河南省农业科学院经济作物研究所 Institute of Economic Crops, Henan Academy of Agricultural Sciences	中棉所12/豫植177 CRI12/Yuzhi 177
衡棉3号 Hengmian 3	河北省农林科学院旱作农业研究所 Dry Farming Agricultural Research Institute, Hebei Academy of Agricultural and Forestry Sciences	衡9273/GK12 Heng 9273/GK12
中棉所41 CRI41	中国农业科学院棉花研究所 Institute of Cotton Research of Chinese Academy of Agricultural Sciences	中棉所23导入国产Bt和CpTI双价抗虫基因 Introduction of Bt and CpTI genes into CRI23
中棉所35 CRI35	中国农业科学院棉花研究所 Institute of Cotton Research of Chinese Academy of Agricultural Sciences	中23021/（中棉所12×川1704） Zhong 23021/(CRI12×Chuang 1704)

基因编号 Gene ID	基因 Gene	基因注释 Gene functional annotations	表达倍数 log₂Ratio (Treatment/Control)	正向引物 Forward primer sequence (5′-3′)	反向引物 Reverse primer sequence (5′-3′)
CotAD_50094	ZAT11	锌指蛋白ZAT11 Zinc finger protein ZAT11	2.714	AACGGAGAACCAACCACCAG	CCCAAGGCTTGTCCGATTGA
CotAD_58358	COR47	脱水素COR47 Dehydrin COR47	2.129	GTTAGCGGTGAAGGAGCAGT	ACTCGGTTACGATCACCTCC
CotAD_22633	ARG2	吲哚-3-乙酸诱导蛋白ARG2 Indole-3-acetic acid-induced protein ARG2	6.762	GCTACGGTGGCCAAGAAAAC	TTGGGACAGTTCTCGGGTCT
CotAD_74365	PBP1	钙信号转导的钙结合蛋白PBP1 Calcium-binding protein PBP1	5.134	CTTGAAGAGGAACGCTGCTG	TTCCATTTCACTGAGAGCCCC
CotAD_45286	CIPK6	CBL相互作用蛋白激酶 CBL-interacting serine/threonine-protein kinase 6	4.209	GTGTACGACTGCAGGGTCAA	ACCAGAAACGACGGTGTCAA
CotAD_09571	MPK3	丝裂原活化蛋白激酶3 Mitogen-activated protein kinase 3	2.244	CTGCTTGGCACTCCAACTGA	TGTTGACGAGGGTATGCAGG
CotAD_12521	EXPA8	细胞壁伸展蛋白A8 Expansin-A8	-7.391	AGGGTTTGGGACCAACACAG	AGCAGGCAAGCACCATTTTG
CotAD_76943	TRG-31	可能的水通道蛋白PIP 7a型 Probable aquaporin PIP-type 7a	3.050	AGGTATGGTGCTTTGGGTGG	CTTGGCATCAGTGGCTGAGA
CotAD_17081	WRKY46	可能的WRKY转录因子46 Probable WRKY transcription factor 46	2.684	CCTCGCTTAGCACGATGGAA	GCCAGAGCGAACCCTAAGTT
CotAD_55002	NAC100	含NAC结构域的蛋白100 NAC domain-containing protein 100	4.545	ACTTCAGTGCCGCTGCTATT	TCCCCCATCTTGGCTTTGTC
CotAD_56948	poxN1	过氧化物酶N1 Peroxidase N1	3.234	TGGAGGACACACCATAGGGA	TTGAGGCACGAATGCTGAGT

名称 Name	株高 Plant height (cm)	单株果枝数 Number of fruit branches per plant	单株铃数 Number of bolls per plant	铃重 Single boll weight (g)	衣分 Lint percentage (%)	籽棉产量 Unginned cotton yield (kg·hm^-2)
TM-1	94.60Aa	12.80A	10.40A	6.55Aa	39.18Aa	2791.50A
中棉所35 CRI35	84.40Ab	9.10B	7.80B	5.65Ab	38.66Aa	2347.20B

处理 Treatments	3 d子叶平展率 The cotyledon spreading rate of 3 d (%)	7 d子叶平展率 The cotyledon spreading rate of 7 d (%)	7 d苗高 Plant height of 7 d (cm)	7 d相对叶绿素含量 The relative chlorophyll content of 7 d (SPAD value)
CK	95.56A	100.00Aa	10.05A	59.72A
4℃	68.12B	94.74Aa	5.62B	39.63B

品种 Variety	处理 Treatments	相对叶绿素含量 The relative chlorophyll content (SPAD value)	耐冷指数 Index of cold tolerance (%)	耐冷级别 Level of cold tolerance
TM-1	CK	53.22Ab
	4℃ 4 d	52.33Ab
	CK恢复7 d Recovery for 7 d of CK	60.2Aa
	4℃4 d恢复7 d Recovery for 7 d after 4℃4 d	53.91Ab	85.32Aa	抗冷Chilling resistant
豫2067 Yu 2067	CK	52.61Ab
	4℃4 d	51.76Ab
	CK恢复7 d Recovery for 7 d of CK	60.8Aa
	4℃4 d恢复7 d Recovery for 7 d after 4℃4 d	54.16Ab	76.55Ab	抗冷Chilling resistant
衡棉3号 Hengmian 3	CK	51.12Ab
	4℃4 d	41.14B
	CK 恢复7 d Recovery for 7 d of CK	60.6Aa
	4℃4 d恢复7 d Recovery for 7 d after 4℃4 d	41.48B	21.62B	冷敏感Chilling sensitive

Characteristics and Cold Tolerance of Upland Cotton Genetic Standard Line TM-1

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