Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (9): 1913-1920.doi: 10.3864/j.issn.0578-1752.2020.09.017

• ANIMAL SCIENCE·VETERINARY SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Study of Genotyping and Performance in Late-Feathering Qingyuan Partridge Cocks

Hua LI1,2,GuiJun FANG1,GuoHong HUA2,ShuWen TAN1,3,ZhengFen ZHANG2,YuYu HONG1,Hui YU1,2   

  1. 1 Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding/Foshan University, Foshan 528225, Guangdong;
    2 Guangdong Tinoo’s Foods Corporation Ltd., Qingyuan 511827, Guangdong
  • Received:2017-11-15 Accepted:2020-03-11 Online:2020-05-01 Published:2020-05-13

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Abstract:

【Objective】In order to verify the genotyping accuracy of heterozygotic cocks, and to construct the resistant strain without ev21 gene, the relationship among genotypes, four late-feathering sub-phenotypes (named micro-type as L1, inverted type as L2, isometric type as L3, and ungrown type as L4) and the production performance were investigated in the late-feathering Qingyuan partridge cocks. The feasibility was demonstrated by the expression profile difference of two candidate genes, i.e., Prolactin Receptor (PRLR) and Sperm Flagellar Protein 2 (SPEF2), between the early- and late-feathering Qingyuan partridge cocks. 【Method】Genotypes were detected by PCR-RFLP in the late-feathering Qingyuan partridge cocks, and its accuracy was verified by progeny testing using a test cross. The production differences among genotyping groups were compared by variance analysis of percentage data and t-test. The expressions of two candidate genes were quantified by real-time quantitative PCR (RT-PCR) in both the early (R2) feathering and late (L2 and L4) feathering cocks. 【Result】Among the 568 late-feathering cocks, the proportion of the deletant group (ev21 - group) was 41.73%, and that of the ev21 + group was 58.27%, of which the homozygotes and the heterozygote accounted for 8.80% and 49.47%, respectively. By the test cross, the genotyping accuracy was 46.83% both in the homozygote group and the deletant group, indicating that the heterozygote was in fact homozygote. The descendant percentage of the isometric late-feathering type in ev21 + group was significantly higher than that in ev21 - group (P≤0.05). The feather maturity of the late-feathering cocks at 105-day-old in the ev21 - group was extremely significantly higher than that in ev21 +group (P≤0.01). At one-day-old chicks, the expression of PRLR gene between R2 versus (L2+L4) showed no any difference (P>0.05),but its expression between R2 versus L4 was significantly down-regulated (0.01<P≤0.05). While the expression of SPEF2 gene in late-feathering chickens was extremely significantly higher than that in early-feathering chickens (P≤0.01). By comparing with the R2 group, SPEF2 significantly differentially up-regulated in the L4 and L2 groups (P≤0.01). 【Conclusion】New genotyping method should be developed for improving the accuracy of the heterozygote in the late feathering cocks. Up to now, a test cross is essential for the chicken production. Construction of the ev21 -resistant strain is viable in late-feathering chicken breeding. Furthermore, studies need to be conducted to figure out the difference of isometric subtype ratio and feather maturity between the ev21 + group and the ev21 - group. Besides, SPEF2 and PRLR were candidate genes for subtypes affecting the difference of early-feathering and late-feathering chickens at 1-day-old.

Key words: Qingyuan partridge cocks, late-feathering, genotyping, performance

Fig. 1

PCR-RFLP results of late-feathering Qingyuan Partridge cocks M represents Marker DL2 000; 2 is homozygote, 5, 8 and 9 are heterozygotes, while 1, 3, 4, 6 and 7 are deletants"

Table 1

Genotyping cocks of the late-feathering line"

群体
Population		 纯合子Homozygote 缺失体Deletant 杂合子Heterozygote 合计Total
No % No % No % No
纯系Pure line 23 10.09 100 43.86 105 46.05 228
祖代Progenitor 27 7.94 137 40.29 176 51.77 340
合计Total 50 8.80 237 41.73 281 49.47 568

Table 2

Comparison of phenotypes and genotypes in late-feathering cocks by test cross"

基因分型组
Genotyping group		 数量 Count 出苗Seedlings 慢羽表型Phenotyes (%)
只 % 只 微长型L1 倒长型L2 等长型L3 未出型L4
纯合子Ⅰ HomozygoteⅠ 4 5.06 34 5.89 70.60 11.76 11.76
杂合子Ⅱ HeterozygoteⅡ 42 53.17 453 13.02 61.59 9.49 15.90
Ⅰ+Ⅱ (ev21+) 46 58.23 487 12.52 62.22 9.65b 15.61
缺失体(ev21-) Deletant 33 41.77 396 10.86 63.39 5.30a 20.45
合计Total 79 100 883 11.78 62.74 7.70 17.78

Table 3

Comparisons of the production performance of the late-feathering cocks at 105-day-old"

基因分型组
Genotyping group		 体重
Weight (g)		 冠高
Crown height (mm)		 胫长
Shank length (mm)		 胫围
Shank circumstance (cm)		 通管值
Feather maturity
ev21+ n=123 1302.6±159.7 43.1±5.4 81.7±2.8 3.9±0.2 40.3±15.7c
缺失体Deletant n=94 1268.5±129.6 42.8±5.6 81.7±3.3 3.9±0.1 47.5±22.5a
合计Total n=217 1287.8±144.7 42.9±5.7 81.7±3.1 3.9±0.1 43.4±20.6

Fig. 2

Expression of PRLR and SPEF2 by RT-PCR A indicating the control group is (L2+L4); B indicating the control group is L4; Gene expression with the same letter indicate not significant difference(P>0.05); Gene expression with adjacent letters was significant difference (0.01<P≤0.05); Gene expression with interphase letters was highly significant difference (P≤0.01)"

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