Scientia Agricultura Sinica ›› 2019, Vol. 52 ›› Issue (23): 4374-4385.doi: 10.3864/j.issn.0578-1752.2019.23.017

;

• SPECIAL FOCUS: MOLECULAR BIOLOGY OF APPLE • Previous Articles     Next Articles

Analysis of Apple Ethylene Response Factor MdERF72 to Abiotic Stresses

WANG JiaHui,GU KaiDi,WANG ChuKun,YOU ChunXiang,HU DaGang(),HAO YuJin()   

  1. College of Horticulture Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology/MOA Key Laboratory of Horticultural Crop Biology(Huanghuai Region)and Germplasm Innovation, Tai’an 271018, Shandong
  • Received:2019-03-21 Accepted:2019-07-01 Online:2019-12-01 Published:2019-12-01
  • Contact: DaGang HU,YuJin HAO E-mail:fap_296566@163.com;haoyujin@sdau.edu.cn

RichHTML

14

PDF

2115

Abstract:

【Objective】Ethylene response factor (ERF) , a plant-specific transcription factor, is involved in the growth and development of root formation, hypocotyl elongation, fruit ripening, and organ senescence. It also plays a vital role in regulating responses of plant biological and abiotic stress, as well as fruit qualities. In this study, we cloned the apple ethylene response factor MdERF72. Subsequently, a series of expression analysis and functional identification of transgenic apple calli were performed to study its role in abiotic stress responses. These results provided a theoretical basis for exploring the functions of MdERF72 in plant growth and development.【Method】Using Orin apple calli (Malus calli stica Borkh.) as the test material, the MdERF72 was cloned from apple fruits by RT-PCR assay. Bioinformatics methods were used to analyze its amino acid sequence, physicochemical properties genetic relationship, and spatial structure. MEGA5.0 was used to construct the phylogenetic tree for analyzing the homology of its protein sequence with ERF-B2 subfamily in Arabidopsis. The real-time fluorescence PCR (qRT-PCR) assays were performed to analyse the expression of MdERF72 in different organs and tissues of apple, as well as in apple fruits during different developmental stages. Meanwhile, the expression of MdERF72 in Gala apple tissue culture seedlings treated with ACC, NaCl and low-temperature was detected by qRT-PCR assay. We also constructed its overexpression vector and obtained stable overexpression apple calli through Agrobacterium-mediated genetic transformation. The fresh weight, malondialdehyde content, electrical conductivity, hydrogen peroxide content and superoxide anion content of the wild type and transgenic apple calli were detected after NaCl and low temperature treatment. 【Result】 MdERF72 was located on chromosome 13 in apple genome, which had an AP2/ERF domain, unique to ERF family. Phylogenetic tree analyses indicated that the apple MdERF72 exhibited the highest sequence similarity to Arabidopsis AtERF72, and belonged to the B2 subfamily of ERF family. Analysis of amino acid physicochemical properties indicated that MdERF72 encodes 253 amino acids, and its protein molecular weight was predicted as 27.61 kD, the isoelectric point (pI) was 5.10. In addition, the pro-hydrophobic prediction showed that the hydrophobic portion of MdERF72 was larger than the hydrophilic portion, indicating that it belonged to a hydrophobic protein. Phosphorylation site analysis revealed that MdERF72 had only one threonine phosphorylation site, suggesting that the protein might be regulated by phosphorylation. The results revealed that the MdERF72 promoter sequence contains cis-acting elements associated with jasmonic acid (JA), auxin and drought signals. qRT-PCR analysis showed that MdERF72 was a positive regulatory transcription factor of ethylene, which was expressed in all tissues of apple. Its expression in fruits and stems was relatively high, and gradually increased with the fruit ripening. The expression of MdERF72 in apple tissue culture seedlings was significantly induced by high salt and low temperature. Under the treatment of high salt and low temperature stresses, the MdERF72- overexpressing apple calli had stronger growth potential than the wild type control, and the conductivity, malondialdehyde, hydrogen peroxide and superoxide anion content were lower than the wild type control, indicating that MdERF72 increased the resistance to salt and low temperature stresses.【Conclusion】MdERF72 played an important role in the regulation of high salt and low temperature stresses. Overexpression of MdERF72 could increase the resistance of apple calli to high salt and low temperature stresses.

Key words: apple, MdERF72, ethylene response factor, stress responses

Fig. 1

PCR amplification of MdERF72"

Fig. 2

Sequence alignment of MdERF72 in apple and its homologous"

Fig. 3

Phylogenetic tree between apple ERF protein MDP0000756341 and Arabidopsis ERF-B2 family proteins"

Table 1

Some important cis-acting regulatory elements in the promoter of MdERF72"

调控序列
Regulatory sequence		 序列
Sequence		 位点功能
Function of site		 位置
Location
Sp1 GGGCGG 光响应元件Light responsive element +51
TGA-box TGACGTAA 生长素响应元件Part of an auxin-responsive element -960
LTR CCGAAA 低温响应元件cis-acting element involved in low-temperature responsiveness -600
ARE AAACCA 厌氧响应元件cis-acting regulatory element essential for the anaerobic induction +1238
MBS CAACTG 干旱胁迫响应元件MYB binding site involved in drought-inducibility +1192
TGACG-motif TGACG 茉莉酸响应元件cis-acting regulatory element involved in the MeJA-responsiveness +768, -841
CAT-box GCCACT 分生组织表达响应元件cis-acting regulatory element related to meristem expression -1986

Fig. 4

Expression analysis of MdERF72 of Gala apple tissue culture seedlings in response to ACC Different letters indicate significant difference at 0.05 levels. The same as below"

Fig. 5

Expression of MdERF72 gene in different tissues of apple"

Fig. 6

Expression of MdERF72 gene in different developmental stages of apple"

Fig. 7

Expression analysis of MdERF72 of Gala apple tissue culture seedlings in response to NaCl (A) and 4℃ (B) low temperature"

Fig. 8

Identification of MdERF72 transgenic apple calli"

Fig. 9

Growth status of wild type (WT) and MdERF72 overexpressing apple calli (OE1, OE2 and OE3) under NaCl"

Fig. 10

Fresh weight (A), Malondialdehyde content (B), electronic conductivity (C), Hydrogen peroxide content(D) and Superoxide anion content (E) of wild type (WT) and MdERF72 overexpressing apple calli (OE1, OE2 and OE3) under NaCl treatments"

Fig. 11

Growth status of wild type (WT) and MdERF72 overexpressing apple calli (OE1, OE2 and OE3) under 4℃ low temperature"

Fig. 12

Fresh weight (A), Malondialdehyde content (B), electronic conductivity (C), Hydrogen peroxide content (D) and Superoxide anion content (E) of wild type (WT) and MdERF72 overexpressing apple calli (OE1, OE2 and OE3) under 4℃ low temperature"

[1] 杨志佳 . 盐碱胁迫下拟南芥14-3-3蛋白对蛋白激酶SOS2和PKS5调控的研究[D]. 北京: 中国农业大学, 2019.
YANG Z J . Regulation of protein kinases SOS2 and PKS5 by 14-3-3 protein under salt and alkali stress in Arabidopsis. Beijing: China Agricultural University, 2019. (in Chinese)
[2] LICAUSI F, OHME-AKAGI M, PERATA P . APETALA 2/Ethylene Responsive Factor (AP2/ERF) transcription factors: Mediators of stress responses and developmental programs. New Phytologist, 2013,199(3):639-649.
doi: 10.1111/nph.12291 pmid: 24010138
[3] RIECHMANN J L, HEARD J, MARTIN G, REUBER L, JIANG C Z, KEDDIE J, ADAM L, PINEDA O, RATCLIFFE O J, SAMAHA R R, CREELMAN R, PILGRIM M, BROUN P, ZHANG J Z, GHANDEHARI D, SHERMAN B K, YU G L . Arabidopsis transcription factors: Genome-wide comparative analysis among eukaryotes. Science, 2000,290(5499):2105-2110.
doi: 10.1126/science.290.5499.2105 pmid: 11118137
[4] NAKANO T, SUZUKI K, FUJIMURA T, SHINSHI H . Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiology, 2006,140(2):411-432.
doi: 10.1104/pp.105.073783 pmid: 16407444
[5] OHME-TAKAGI M, SHINSHI H . Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. The Plant Cell, 1995,7(2):173-182.
doi: 10.1105/tpc.7.2.173 pmid: 7756828
[6] MOOSE S P, SISCO P H . Glossy15, an APETALA2-like gene from maize that regulates leaf epidermal cell identity. Genes & Development, 1996,10(23):3018-3027.
doi: 10.1055/s-0039-3400233 pmid: 31842235
[7] GU Y Q, WILDERMUTH M C, CHAKRAVARTHY S, LOH Y T, YANG C, HE X H, MARTIN G B . Tomato transcription factors Pti4, Pti5, and Pti6 activate defense responses when expressed in Arabidopsis. The Plant Cell, 2002,14(4):817-831.
doi: 10.1105/tpc.000794 pmid: 11971137
[8] MITO T, SEKI M, SHINOZAKI K, TAKAGI M O, MATSUI K . Generation of chimeric repressors that confer salt tolerance in Arabidopsis and rice. Plant Biotechnology Journal, 2011,9(7):736-746.
doi: 10.1111/j.1467-7652.2010.00578.x
[9] JAGLO K R, KLEFF S, AMUNDSEN K L, ZHANG X, HAAKE V, ZHANG J Z, DEITS T, THOMASHOW M F . Components of the Arabidopsis C-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species. Plant Physiology, 2001,127(3):910-917.
pmid: 11706173
[10] NOVILLO F, MEDINA J, SALINAS J . Arabidopsis CBF1 and CBF3 have a different function than CBF2 in cold acclimation and define different gene classes in the CBF regulon. Proceedings of the National Academy of Sciences of the USA, 2007,104(52):21002-21007.
doi: 10.1073/pnas.0705639105 pmid: 18093929
[11] HSIEH T H, LEE J T, YANG P T, CHIU L H, CHARNG Y Y, WANG Y C, CHAN M . Heterology expression of the Arabidopsis C-repeat/ dehydration response element binding Factor 1 gene confers elevated tolerance to chilling and oxidative stresses in transgenic tomato. Plant Physiology, 2002,129(3):1086-1094.
doi: 10.1104/pp.003442 pmid: 12114563
[12] KASUGA M, MIURA S, SHINOZAKI K, SHINOZAKI K Y . A combination of the Arabidopsis DREB1A gene and stress-inducible rd29A promoter improved drought-and low-temperature stress tolerance in tobacco by gene transfer. Plant and Cell Physiology, 2004,45(3):346-350.
doi: 10.1093/pcp/pch037 pmid: 15047884
[13] ITO Y, KATSURA K, MARUYAMA K, TAJI T, KOBAYASHI M, SEKI M, SHINOZAKI K, SHINOZAKI K Y . Functional analysis of rice DREB1/CBF-type transcription factors involved in cold-responsive gene expression in transgenic rice. Plant and Cell Physiology, 2006,47(1):141-153.
doi: 10.1093/pcp/pci230 pmid: 16284406
[14] PINO M T, SKINNER J S, JEKNIĆ Z, HAYES P M, SOELDNER A H, THOMASHOW M F, CHEN T H H . Ectopic AtCBF1 over- expression enhances freezing tolerance and induces cold acclimation- associated physiological modifications in potato. Plant, Cell & Environment, 2008,31(4):393-406.
doi: 10.1111/j.1365-3040.2008.01776.x pmid: 18182016
[15] LIU Q, KASUGA M, SAKUMA Y, ABE H, MIURA S, YAMAGUCHI- HINOZAKI K, SHINOZAKI K . Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature- responsive gene expression, respectively, in Arabidopsis. The Plant Cell, 1998, 10(8):1391-1406.
doi: 10.1105/tpc.10.8.1391 pmid: 9707537
[16] CHEN J R, LÜ J J, LIU R, LIU R, XIONG X Y, WANG T X, CHEN S Y, GUO L B, WANG H F . DREB1C from Medicago truncatula enhances freezing tolerance in transgenic M. truncatula and China Rose (Rosa chinensis Jacq.). Plant Growth Regulation, 2010,60(3):199-211.
doi: 10.1007/s10725-009-9434-4
[17] ZHANG Z, HUANG R . Enhanced tolerance to freezing in tobacco and tomato overexpressing transcription factor TERF2/LeERF2 is modulated by ethylene biosynthesis. Plant Molecular Biology, 2010,73(3):241-249.
doi: 10.1007/s11103-010-9609-4
[18] TRUJILLO L E, SOTOLONGO M, MENENDEZ C, OCHOGAVÍA M E, COLL Y, HERNÁNDEZ I, HIDALGO O B, THOMMA B P H J, VERA P, HERNÁNDEZ L . SodERF3, a novel sugarcane ethylene responsive factor (ERF), enhances salt and drought tolerance when overexpressed in tobacco plants. Plant and Cell Physiology, 2008,49(4):512-525.
doi: 10.1093/pcp/pcn025 pmid: 18281696
[19] SERRA T S, FIGUEIREDO D D, CORDEIRO A M, ALMEIDA D M, LOURENÇO T, ABREU I A, SEBASTIÁN A, FERNANDES L, MOREIRA B C, OLIVEIRA M M, SAIBO N J M . OsRMC, a negative regulator of salt stress response in rice, is regulated by two AP2/ERF transcription factors. Plant Molecular Biology, 2013,82(4/5):439-455.
doi: 10.1007/s11103-013-0073-9 pmid: 23703395
[20] JUNG J, WON S Y, SUH S C, KIM H, WING R, JEONG Y, HWANG I, KIM M . The barley ERF-type transcription factor HvRAF confers enhanced pathogen resistance and salt tolerance in Arabidopsis. Planta, 2007,225(3):575-588.
doi: 10.1007/s00425-006-0373-2
[21] KIM Y H, JEONG J C, PARK S, LEE H S, KWAK S S . Molecular characterization of two ethylene response factor genes in sweetpotato that respond to stress and activate the expression of defense genes in tobacco leaves. Journal of Plant Physiology, 2012,169(11):1112-1120.
doi: 10.1016/j.jplph.2012.03.002
[22] 刘文奇, 陈旭君, 徐晓晖, 凌建群, 郭泽建 . ERF类转录因子OPBP1基因的超表达提高烟草的耐盐能力. 植物生理与分子生物学学报, 2002,28(6):473-478.
LIU W Q, CHEN X J, XU X H, LING J Q, GUO Z J . Overexpression of ERF transcription factor OPBP1 gene enhances salt tolerance of tobacco. Journal of Plant Physiology and Molecular Biology. 2002,28(6):473-478. (in Chinese)
[23] SHIN R, PARK J M, AN J M, PAEK K H . Ectopic expression of Tsi1 in transgenic hot pepper plants enhances host resistance to viral, bacterial, and oomycete pathogens. Molecular Plant-Microbe Interactions, 2002,15(10):983-989.
doi: 10.1094/MPMI.2002.15.10.983 pmid: 12437295
[24] 刘伟 . 乙烯响应因子ERF4/ERF72参与苹果砧木缺铁应答的功能研究[D]. 北京: 中国农业大学, 2017.
LIU W . Functional research of ethylene response factor ERF4/ERF72 involved in iron deficiency response of apple rootstocks. Beijing: China Agricultural University, 2017. (in Chinese)
[25] 韩朋良, 刘肖娟, 刘鑫, 董元花, 胡大刚, 郝玉金 . 苹果生长素阻遏蛋白基因MdIAA26的分子克隆与功能鉴定. 园艺学报, 2018,45(6):1041-1053.
HAN P L, LIU X J, LIU X, DONG Y H, HU D G, HAO Y J . Molecular cloning and functional identification of apple auxin repressor protein gene MdIAA26. Acta Horticulturae Sinica, 2018,45(6):1041-1053. (in Chinese)
[26] 张全艳, 于建强, 王佳慧, 胡大刚, 郝玉金 . 苹果MdNAC143的克隆及其在苹果愈伤组织的抗盐功能鉴定. 园艺学报, 2017,44(11):2163-2170.
ZHANG Q Y, YU J Q, WANG J H, HU D G, HAO Y J . Molecular cloning and functional characterization of MdNAC143 reveals its involvement in salt tolerance in apple callus. Acta Horticulturae Sinica, 2017,44(11):2163-2170. (in Chinese)
[27] HU D G, SUN C H, MA Q J, YOU C X, CHENG L, HAO Y J . MdMYB1 regulates anthocyanin and malate accumulation by directly facilitating their transport into vacuoles in apples. Plant Physiology, 2016,170(3):1315-1330.
doi: 10.1104/pp.15.01333 pmid: 26637549
[28] 赵世杰, 许长成, 邹琦, 孟庆伟 . 植物组织中丙二醛测定方法的改进. 植物生理学通讯, 1994,30(3):207-210.
ZHAO S J, XU C C, ZOU Q, MENG Q W . Improvements of method for measurement of malondialdehyde in plant tissues. Plant Physiology Communications, 1994,30(3):207-210. (in Chinese)
[29] 崔之益, 李蕊萍, 胡加新, 奚如春 . 电导法在植物研究中应用. 安徽农业科学, 2014,42(17):5358-5359, 5366.
CUI Z Y, LI R P, HU J X, XI R C . Application of conductivity method in botanical research. Journal of Anhui Agricultural Sciences, 2014,42(17):5358-5359, 5366. (in Chinese)
[30] OH S J, SONG S I, KIM Y S, JANG H J, KIM S Y, KIM M, KIM Y K, NAHM B H, KIM J K . Arabidopsis CBF3/DREB1A and ABF3 in transgenic rice increased tolerance to abiotic stress without stunting growth. Plant Physiology, 2005,138(1):341-351.
doi: 10.1104/pp.104.059147 pmid: 15834008
[31] HONG B, TONG Z, MA N, KASUGA M, SHINOZAKI Y, GAO J P . Expression of the Arabidopsis DREB1A gene in transgenic chrysanthemum enhances tolerance to low temperature. The Journal of Horticultural Science and Biotechnology, 2006,81(6):1002-1008.
doi: 10.1016/j.plaphy.2014.03.030 pmid: 24751398
[32] HONG B, TONG Z, MA N, LI J, KASUGA M, YAMAGUCHI S K, GAO J P . Heterologous expression of the AtDREB1A gene in chrysanthemum increases drought and salt stress tolerance. Science in China Series C: Life Sciences, 2006,49(5):436-445.
doi: 10.1007/s11427-006-2014-1 pmid: 17172050
[33] FISCHER U, DRÖGE-LASER W . Overexpression of NtERF5, a new member of the tobacco ethylene response transcription factor family enhances resistance to tobacco mosaic virus. Molecular Plant- Microbe Interactions, 2004,17(10):1162-1171.
doi: 10.1094/MPMI.2004.17.10.1162 pmid: 15497409
[34] ZUO K J, QIN J, ZHAO J Y, LING H, ZHANG L D, CAO Y F, TANG K X . Over-expression GbERF2 transcription factor in tobacco enhances brown spots disease resistance by activating expression of downstream genes. Gene, 2007,391(1/2):80-90.
doi: 10.1016/j.gene.2006.12.019 pmid: 17321073
[35] LI T, XU Y X, ZHANG L C, JI Y L, TAN D M, YUAN H, WANG A D . The jasmonate-activated transcription factor MdMYC2 regulates ETHYLENE RESPONSE FACTOR and ethylene biosynthetic genes to promote ethylene biosynthesis during apple fruit ripening. The Plant Cell, 2017,29(6):1316-1334.
doi: 10.1105/tpc.17.00349 pmid: 28550149
[36] MÜLLER M, MUNNÉ-BOSCH S . Ethylene response factors: A key regulatory hub in hormone and stress signaling. Plant Physiology, 2015,169(1):32-41.
doi: 10.1104/pp.15.00677 pmid: 26103991
[1] SU YiFan, YANG ZhanXu, WANG Di, MAO JunCheng, WEI MengMeng, CHEN Ze, BAI XinRan, CHU TianGe, MA ChangNing, QIAO MingFei, SUN Quan, HU DaGang. Effects of 2, 4-Epibrassinolide on Postharvest Storage Quality and Physiological Performance of Apple [J]. Scientia Agricultura Sinica, 2026, 59(7): 1536-1551.
[2] CONG QiQi, ZHANG JingYi, MENG XiangLong, DAI PengBo, LI Bo, HU TongLe, WANG ShuTong, CAO KeQiang, WANG YaNan. Identification of Hypovirus in Apple Ring Rot Fungus Botryosphaeria dothidea and Detection of Virus-Carrying Status in China [J]. Scientia Agricultura Sinica, 2025, 58(3): 478-492.
[3] PAN Yuan, WANG De, LIU Nan, MENG XiangLong, DAI PengBo, LI Bo, HU TongLe, WANG ShuTong, CAO KeQiang, WANG YaNan. Evaluation of the Effectiveness of Two High-Throughput Sequencing Techniques in Identifying Apple Viruses and Identification of Two Novel Viruses [J]. Scientia Agricultura Sinica, 2025, 58(2): 266-280.
[4] ZHANG Yi, LIU Ying, CHENG CunGang, LI YanQing, LI Zhuang. Effects of Combined Application Proportion of Cow Manure and Chemical Fertilizer on Soil Organic Carbon Pool and Enzyme Activity in Apple Orchard [J]. Scientia Agricultura Sinica, 2024, 57(20): 4107-4118.
[5] ZHOU HanMi, MA LinShuang, SUN QiLi, CHEN JiaGeng, LI JiChen, SU YuMin, CHEN Cheng, WU Qi. Optimization of Integrated Water and Nitrogen Regulation System in Apple Based on Multi-Objective Comprehensive Evaluation [J]. Scientia Agricultura Sinica, 2024, 57(18): 3654-3670.
[6] ZENG YanXin, GONG HaoNan, YOU ChunXiang, LU JingSheng, GAO WenSheng, WANG XiaoFei. Effects of Different Rootstocks on Growth and Fruit Quality of Young Ruixianghong Apple Trees with Multi-Stem Shape [J]. Scientia Agricultura Sinica, 2024, 57(14): 2847-2861.
[7] ZHANG HaiQing, ZHANG HengTao, GAO QiMing, YAO JiaLong, WANG YaRong, LIU ZhenZhen, MENG XiangPeng, ZHOU Zhe, YAN ZhenLi. Transcriptome Analysis for Screening Key Genes Related to Regulating Branching Ability in Apple [J]. Scientia Agricultura Sinica, 2024, 57(10): 1995-2009.
[8] SUN Zheng, LAI ZhongXiao, ZHAO XiaoMin, JIANG ZhiLi, CHEN GuangYou, MA ZhiQing. Application Evaluation of the Whole-Process Biological Management Scheme for Apple Pests in the Weibei Dry Highland [J]. Scientia Agricultura Sinica, 2023, 56(6): 1102-1112.
[9] ZHENG WenYan, CHANG YuanSheng, HE Ping, HE XiaoWen, WANG Sen, GAO WenSheng, LI LinGuang, WANG HaiBo. Development and Validation of KASP Markers Based on a Whole- Genome Resequencing Approach in a Hybrid Population of Luli × Red No. 1 [J]. Scientia Agricultura Sinica, 2023, 56(5): 935-950.
[10] WANG ZiDun, WANG Hui, FENG YuChen, ZHANG XueLiang, YAN LeiYu, LIU XiaoJie, ZHAO ZhengYang. Effects of Different Color Fruit Bags on Quality of Ruixue Apple Fruits [J]. Scientia Agricultura Sinica, 2023, 56(4): 729-740.
[11] LI XingXing, ZHOU GuoFu, LUO GuanYu, CHEN SiRong, ZHANG JinLong, CHEN GuoHua, ZHANG XiaoMing. Selection Preference and Adaptability of Bactrocera dorsalis to Different Varieties of Malus pumila [J]. Scientia Agricultura Sinica, 2023, 56(17): 3358-3371.
[12] LI MinJi, LI XingLiang, ZHANG Qiang, ZHOU Jia, YANG YuZhang, ZHOU BeiBei, ZHANG JunKe, WEI QinPing. Effects of Different Distances from Original Planting Row on Tree Growth and Fruit Yield of Young Trees of G935 Dwarf Rootstock Miyato Fuji Under Continuous Cropping [J]. Scientia Agricultura Sinica, 2023, 56(17): 3412-3419.
[13] FU Shan, LIANG Ye, XU JiuLiang, RUAN YunZe, LUO Jian, LI TingYu. Comprehensive Evaluation of Fruit Texture and Taste Quality of Pineapple Based on Multiple Methods [J]. Scientia Agricultura Sinica, 2023, 56(15): 3006-3019.
[14] LI JiaQi, XUN Mi, SHI JunYuan, SONG JianFei, SHI YuJia, ZHANG WeiWei, YANG HongQiang. Response Characteristics of Rhizosphere and Root Endosphere Bacteria and Rhizosphere Enzyme Activities to Soil Compaction Stress in Young Apple Tree [J]. Scientia Agricultura Sinica, 2023, 56(13): 2563-2573.
[15] DONG YongXin,WEI QiWei,HONG Hao,HUANG Ying,ZHAO YanXiao,FENG MingFeng,DOU DaoLong,XU Yi,TAO XiaoRong. Establishment of ALSV-Induced Gene Silencing in Chinese Soybean Cultivars [J]. Scientia Agricultura Sinica, 2022, 55(9): 1710-1722.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd