不同红蓝配比的光质对棉花体细胞胚胎发生 和植株再生的影响

doi:10.3864/j.issn.0578-1752.2019.06.002

Abstract

Abstract:

【Objective】Different light quality combinations were set up to clarify the effects of different light quality on cotton somatic embryogenesis, and to provide basis for improving cotton somatic embryogenesis ability and accelerating cotton genetically modified process. 【Method】The light quality combinations B﹕R (blue﹕red) = 1﹕1, B﹕R (blue﹕red) = 3﹕1, B﹕R (blue﹕red) = 1﹕3 and DL (fluorescent lamp) with different red and blue ratios were set respectively. The hypocotyl segments of CCRI12 cotton were cultured on the callus induction medium (CIM), embryogenic callus induction medium (ECDM), somatic embryo induction medium (EIM) and root induction medium (RIM) in turn. Under four different light quality ratios, the corresponding indicators of somatic embryogenesis at different stages were counted, including callus proliferation rate (CPR), callus proliferation, embryogenic callus differentiation rate, somatic embryo number, rooting rate, hypocotyl number, regenerated cotton plant number, chlorophyll concentration, etc.【Result】Compared with B﹕R=1﹕3 and DL light quality combinations, B﹕R=1﹕1 and B:R=3﹕1 light quality combinations significantly promoted callus proliferation. Compared with B﹕R=1﹕1 and B﹕R=3﹕1 light quality combinations, B﹕R=1﹕3 light quality combination significantly increased weight proliferation of callus without EC differentiation ability. B﹕R=1﹕3 and DL light quality combinations had significantly higher embryonic callus differentiation rate than B﹕R=1﹕1 and B﹕R=3﹕1 light quality combinations. The number of somatic embryos induced by B﹕R=1﹕3 treatment was significantly higher than that of B﹕R=1﹕1 and B:R=3﹕1 light quality combination, but there was no significant difference compared with DL; the number of hypocotyls induced by B﹕R=1﹕1 and B﹕R=3﹕1 light quality combination was significantly lower than that induced by B﹕R=1﹕3 and DL light quality combination, and the number of somatic embryos induced by B﹕R=1﹕1 light quality combination and B﹕R=3﹕1 light quality combination was significantly lower than that induced by B﹕R=1 light quality combination and B﹕R=3﹕1 light. The plant height of B﹕R=1﹕1 and B﹕R=1﹕3 combinations was significantly higher than that of B﹕R=1﹕3 and DL combinations; the chlorophyll content of B﹕R=1﹕3 treatment was higher than that of B﹕R=1﹕1 treatment, but the chlorophyll content of B﹕R=1﹕3 treatment was significantly higher than that of B﹕R=3﹕1 and DL light quality combination.【Conclusion】The regulation of different light quality combinations in different stages of somatic embryogenesis was clarified. Optimal light quality combinations could be selectively applied in different stages of somatic embryogenesis. The combination of red and blue B﹕R=1﹕3 can promote the somatic embryogenesis of cotton.

Key words: Gossypium hirsutum, red and blue ratio, somatic embryogenesis, callus, embryogenic callus, somatic embryo, regenerated seedlings

WEI Xi,WANG QianHua,GE XiaoYang,CHEN YanLi,DING YanPeng,ZHAO MingZhe,LI FuGuang. Effects of Different Red and Blue Ratios on the Somatic Embryogenesis and Plant Regeneration of Cotton[J].Scientia Agricultura Sinica, 2019, 52(6): 968-980.

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名称 Name	基础培养基 MSB (mL·L^-1)	蔗糖 Sucrose (g·L^-1)	葡萄糖 Glucose (g·L^-1)	6-糠氨基嘌呤 Kinetin (KT) (mg·L^-1)	2,4-二氯苯氧乙酸 2,4-D (mg·L^-1)	吲哚乙酸 IAA (mg·L^-1)	6-苄氨基腺嘌呤 6-BA (mg·L^-1)	琼脂 Gelose (g·L^-1)
愈伤诱导培养基CIM	65		28	0.1	0.1			2.2
胚性愈伤诱导培养基ECDM	65		28	0.15		0.05		2.2
体细胞胚诱导培养基EIM	65	28		0.1			0.2	2.2
再生苗诱导培养基RIM	65	25				0.05	0.05

Effects of Different Red and Blue Ratios on the Somatic Embryogenesis and Plant Regeneration of Cotton

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