小麦秆锈病新抗源及抗病基因所在染色体特异分子标记

doi:10.3864/j.issn.0578-1752.2018.07.001

Abstract

Abstract: 【Objective】Wheat stem rust, caused by Puccinia graminis Pers. f. sp. tritici Eriks. and E. Henn (Pgt) is one of the most potentially destructive wheat diseases, seriously threatening world wheat production. The emergence of new races Ug99 of stem rust caused the global wheat to be under the threat.Exploring new resistant source of wheat stem rust is one of the effective measures against Ug99. In order to explore stem rust new resistant source, the mixed dominant stem rust physiological races in China were inoculated to 165 wheat-alien species chromosome lines at the seeding stages. 【Method】All of the 165 wheat-alien species chromosome lines, 3 hexaploid wheat and the susceptible control Little Club (LC), were sown in the 10 cm diameter clay pots. When the primary leaves were fully expanded, they were inoculated using talcurediospore powder mixture of the common races 34MKGQM and 21C3CTHSM. The Infection Types (ITs) of the material tested was recorded according to the standard ‘0-4’. Meanwhile, genomic DNA was extracted from stem rust immune, nearly immune, or highly resistant additions/substitutions and their corresponding whole set of chromosome lines and Chinese Spring (CS). PCR was performed on these materials by screening 101 pairs of PLUG primer. PCR products were firstly digested by DNA restricted enzymes TaqI and HaeⅢ, and then were detected through 2.0% agarose gel electrophoresis to screen and establish chromosome-specific molecular marker where the stem rust resistance gene was located on.【Result】Among the 165 wheat-alien species chromosome lines, CS-Ae. geniculata 7M^g#1 addition, CS-Ae. geniculata 7M^g#1(7A) and 7M^g#1 (7B) substitutions, CS-Imperial rye 1R addition, CS-Th. intermedium ?Ai addition (? indicates homoeologous group of the alien chromosomes in wheat background was not identified), CS-Ae. uniaristata 6N addition, CS-Ae. variabilis 6S^vS telosomic addition, CS-Chile barley 6H^ch addition are immune or nearly immune to stem rust. ALCD-Ae. caudata 7C#1 addition, CS-Ae. geniculata 7M^g#1(7D) substitution, CS-Imperial rye 6R addition, CS-Ae. longissima 6S^l#3 addition, CS-Ae. longissima 6S^l#2(6B) substitution, CS-Ae. searsii 3S^s#1 addition, CS-Ae. speltoides 2S#3 addition are highly resistant to stem rust. Comparative analysis of chromosomal locations of stem rust resistant genes indicates that chromosomes 6S^l#2 and 6S^l#3 of Aegilops longissima, chromosome 6R of Imperial rye, chromosome 6H^chof Chile barley, chromosome 7M^g#1 of Ae. geniculata, chromosome7C of Ae. caudate and ?Ai of Th. intermedium may harbor new stem rust resistance gene (s). Molecular marker screening, localization, specificity verification showed that 8 new molecular markers have been developed. Among them, 5 (TNAC1715, TNAC1718, TNAC1737, TNAC1739 and TNAC1753) and 3 (TNAC1740, TNAC1751, and TNAC1756) have been assigned to chromosomes 6R and 6S^l, respectively. 【Conclusion】 Eight materials which probably contain new gene(s) against stem rust were obtained, and eight new chromosome specific molecular markers for stem rust resistance gene(s) were established.

Key words: wheat alien species, chromosome lines, stem rust, molecular marker

HAN Ran, LI TianYa, GONG WenPing, LI HaoSheng, SONG JianMin, LIU AiFeng, CAO XinYou, CHENG DunGong, ZHAO ZhenDong, LIU Cheng, LIU JianJun. New Resistance Sources of Wheat Stem Rust and Molecular Markers Specific for Relative Chromosomes That the Resistance Genes are Located on[J].Scientia Agricultura Sinica, 2018, 51(7): 1223-1232.

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