硼胁迫对枳橙砧木细根根尖成熟区和幼嫩叶片细胞结构的影响

doi:10.3864/j.issn.0578-1752.2015.24.010

Abstract

Abstract: 【Objective】The aim of this study was to determine the effects of boron deficiency on the cellular structures of the maturation zone in root tips and the functional leaves in citrus. 【Method】A hydroponic experiment was conducted with (10 μmol·L-1 H3BO3, +B) and without boron (-B) treatment. Fine root mature zone and young leaf samples were observed by paraffin sections and transmission electron microscope (TEM). 【Result】Boron deficiency reduced the amount of parenchyma cell in root and, arranged loosely, led to cell deformation and enlarged the intercellular space. Also, boron deficiency caused the disintegration of the cytoplasm and organelles, and increased the cell wall. Whereas the parenchyma cell morphology was normal, evenly sized, and arranged densely in +B treatment, the parafin section showed the root vascular bundle was dyed deeper and the structure was clearly organized in a circular pattern around the root pulp. Low B stress seriously inhibited the development of vascular bundle and restricted its differentiation, making the cell small and randomly arranged. In the plant, Boron leakage made the leaves thick and uneven and sponge tissue became deformed, leading to bigger cell gaps and an increase in the number and volume of sponge tissue. This improved the proportion of sponge tissue in mesophyll. However, in B+ treatment, leaf thickness was more even and the epidermis cell was more densely arranged. The palisade mesophyll was closely packed and sponge tissue underneath was loosely and organically arranged. Under B deficiency, there was also an accumulation of starch grains in the cell and leaf soluble sugar and starch content increased by 35.3% and 66.7%, respectively. But there was no obvious accumulation of starch in the leaves with +B treatment. 【Conclusion】 The internal structure of the leaf cell was destroyed by boron deficiency. Cell wall thickness and starch accumulation intensified while sponge tissue showed obvious proliferation. The leaf’s abnormal growth further inhibited the development of leaf and root vascular bundles.

Key words: trifoliate orange rootstock, boron deficiency, root tip, leaf, anatomical structure, subcellular structure

LIU Lei-chao, JIANG Cun-cang, DONG Xiao-chang, WU Xiu-wen, LIU Gui-dong, LU Xiao-pei. Effects of Boron Deficiency on Cellular Structures of Maturation Zone from Root Tips and Functional Leaves from Middle and Upper Plant in Trifoliate Orange Rootstock[J].Scientia Agricultura Sinica, 2015, 48(24): 4957-4964.

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Effects of Boron Deficiency on Cellular Structures of Maturation Zone from Root Tips and Functional Leaves from Middle and Upper Plant in Trifoliate Orange Rootstock

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