国家作物种质库油菜种子发芽和出苗监测研究

doi:10.3864/j.issn.0578-1752.2011.07.002

Abstract

Abstract: 【Objective】The objective was to study seed germination and seedling emergence of rape accessions stored in the National Crop Genebank of China (NCGC), and to realize the key factors on germinating ability variation of seeds including initial germination percentage level and storage-time, and to assess the effect of storage on rapeseed wild relatives, and to provide a scientific basis for monitoring seed viability and making renewal protocols of rapeseed and other crops stored in the NCGC. 【Method】 Seed germination and seedling emergence of 347 rapeseed accessions stored in the NCGC for 8-22 years were monitored. The effect of storage on stored seeds of different initial germination percentages, storage-time and germplasm-type were analyzed with Pearson Correlation, T-test and One-way ANOVA by the software SPSS 10.0.【Result】The results showed that after 8-22 years’ storage in the NCGC, more than 83.6% of the 347 monitored accessions of rape seeds maintained a germination percentage above 85%, the lowest initial viability standard for rape cultivated species seeds when put into a low-temperature genebank. The germination percentage of 29 accessions, accounting for 8.3% of the total, declined significantly from above 80% to below 70%. The germination percentage of 142 accessions, accounting for 40.9% of the total, declined significantly (α=2.5%).The ratio of significant declining (α=2.5%) of germination percentages increased along with the declining of initial germination percentage level or with the extending of storing-time of rapeseed as a whole. After above 15 years of storage, the seed germination percentage of rape accessions stored in the NCGC significantly declined. The ratio of significant declining of germination percentages of rapeseed wild relatives was remarkably higher than that of cultivated rapeseed species.【Conclusion】For the safe storage of germplasm resources, the initial germination percentages standard should be above 95% for most of the rapeseeds in the NCGC. The seeds of wild rape relatives or that being stored for above 15 years in the NCGC should be monitored intensively and arranged timely for breeding.

Key words: rape , seeds , storage , germination , seedling emergence , national crop genebank of China

CHEN Bi-yun; ZENG Chang-li;LU Xin-xiong; FU Zhong; CHEN Xiao-ling; WANG Hong-feng; ZHANG Tian-yao; WU Xiao-ming. Monitoring of Rape Seed Germination and Seedling Emergence After Long-Term Storage in the National Crop Genebank of China[J].Scientia Agricultura Sinica, 2011, 44(7): 1315-1322.

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Monitoring of Rape Seed Germination and Seedling Emergence After Long-Term Storage in the National Crop Genebank of China

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