Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (3): 534-545.doi: 10.3864/j.issn.0578-1752.2015.03.13

• HORTICULTURE • Previous Articles     Next Articles

Growth Status, Root Morphology andPhysiologicalCharacteristics of Four Citrus Rootstocks Under Different Phosphorus Levels

FAN Wei-guo, LUO Yan   

  1. Guizhou Fruits Engineering Technology Research Centre/Research Institute for Fruit Resources of Karst Mountain Region, Guizhou University, Guiyang 550025
  • Received:2014-07-30 Online:2015-01-31 Published:2015-01-31

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Abstract: 【Objective】Effects of different phosphorus (P) levels on the plants growth, roots morphology and related physiological characteristics of citrus rootstocks were studied for providing a scientific basis of high P efficiency citrus rootstocks excavation.【Method】The wild Citrus ichangensis Swingle, C. aurantium L., C. limonia Osbeck and cultivated species Poncirus trifoliata Raf. in Guizhou Karst mountain were used as materials, and the date including the growth and biomass, root morphological characteristics, activity of acid phosphatase (APase) and nitrate reductase (NR) of leaves and roots, and secretory acid phosphatase (SAPase) of roots and root absorption kinetics of P characteristics were researched through sand cultivation.【Result】The growth and development of C. ichangensis Swingle was less inhibited under 5 mg•L-1 P level, followed by C. limonia Osbeck and C. aurantium L., restrained intensively to P. trifoliate Raf.. When P concentrations of nutrient solution was 25 mg•L-1, the amount including the plant height, base diameter and fresh weight and dry weight of biomass, total root length, total surface area, total volume and total root tip reached the maximum for C. ichangensis Swingle and C. limonia Osbeck, followed by 5 mg•L-1 P level, 45 mg•L-1 P level was the minimum. With the P concentrations of nutrient solution increasing, the data including the plant height, base diameter and fresh weight and dry weight of biomass, total root length, total surface area, total volume, total root tip amount and root-shoot ratio of C. aurantium L. and P. trifoliata Raf. increased, however, the root-shoot ratio of C. ichangensis Swingle and C. limonia Osbeck decreased. Under low P conditions, C. ichangensis Swingle had the biggest lateral root roughness and the second lateral roots amount and root-shoot ratio, the NR activity in leaves was all in the order of C. ichangensis Swingle ＞C. limonia Osbeck ＞C. aurantium L. ＞P. trifoliata Raf., the NR activity in roots was all in the order of C. aurantium L. ＞C. limonia Osbeck ＞ C. ichangensis Swingle＞P. trifoliata Raf.. The NR activity in leaves and roots of different citrus rootstocks receded with P levels decreased significantly. The APase activity in leaves and roots, and the SAPase activity in roots was all in the order of C. ichangensis Swingle＞C. limonia Osbeck＞C. aurantium L. ＞P. trifoliata Raf. under low P stress. The Imax of C. ichangensis Swingle was greater than C. limonia Osbeck , C.aurantium L. and P. trifoliata Raf. under low P conditions, while the Km and Cmin was smaller than C. limonia Osbeck, C. aurantium L. and P. trifoliata Raf.【Conclusion】The ability to endure to low P stress of C. ichangensis Swingle was much stronger, and response of root morphology and physiological adaptation mechanisms were more obvious than the other three kinds of citrus rootstocks under low P conditions, which showed strong absorption characteristics of P dynamics.

Key words: citrus rootstock, phosphorus, growth, root morphology, physiological characteristics

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