Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (3): 495-502.doi: 10.3864/j.issn.0578-1752.2014.03.009

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

A Research on Response of Enzyme Activities to Long-term Fertilization in Lime Concretion Black Soil

 CHEN  Huan-1, LI  Wei-1, ZHANG  Cun-Ling-2, QIAO  Yu-Qiang-1, DU  Shi-Zhou-1, ZHAO  Zhu-1, CAO  Cheng-Fu-1   

  1. 1、Crop Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031;
    2、Science and Technology Association of Suixi County, Huaibei 235100, Anhui
  • Received:2013-07-11 Online:2014-02-01 Published:2013-12-18

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Abstract: 【Objective】In order to provide a theoretical support for improving soil biochemical environment and soil quality, insuring high and steady yield of crops in view of the characters of “drought, waterlogging, concretion, leanness” in lime concretion black soil, the responsive mechanism of soil enzyme activities to fertilization in black soil was studied by the way of exploring effects of long-term fertilization on soil enzyme activities. 【Method】 Based on a 31 yeas long-term fertilization experiment in Yangliu of Anhui province, the change trend of activities of urease, acid phosphatase, neutral phospahtase, catalase and invertase in tilth soil (0-20 cm) was investigated with 5 patterns of fertilization (non-fertilization, application of single chemical fertilizer, application of single organic fertilizer, mixed application of organic and chemical fertilizer (with the same amount of nitrogen), mixed application of organic and chemical fertilizer (with the lager amount of nitrogen) in wheat-maize rotation system, as well as the correlation and paired comparison was analyzed to interpret the relationship among soil enzymes and seasonal variation, respectively. 【Result】 Compared with control, the activities of urease, catalase, and invertase increased significantly by 130.5% and 129.5%, 11.4% and 14.9%, and 31.4% and 12.0%, respectively, after wheat and maize harvested in the situation of long-term application of single organic fertilizer, while acid and neutral phosphatase were less affected. Long-term application of single chemical fertilizer effectively enhanced soil acid and neutral phosphatase activities, whereas other 3 enzymes activities were significantly inhibited in the same time. With the same amount of nitrogen, the activities of all the 5 enzymes were maintained at a higher level under mixed application of organic and chemical fertilizer, in which low activity of certain enzyme caused by unbalance fertilizing could be avoided. With the lager amount of nitrogen, mixed application of organic and chemical fertilizer increased acid and neutral phosphatase activities significantly, but the effect on urease, catalase and invertase activities was insignificant. There were mutual stimulations between urease and catalase, urease and invertase, catalase and invertase, though phosphatase had a negative correlation with other enzymes. In addition, the seasonal variation of urease, acid and neutral phosphatase, catalase, invertase activities in lime concretion black soil was significantly different in 5 fertilizer applications treatments. 【Conclusion】 With the same amount of nitrogen, mixed application of organic and chemical fertilizer was superior to application of single chemical or single organic fertilizer which made soil enzymes activities stay at a higher level and then will be beneficial to improvement of soil biochemical environment. The correlation of urease and catalase, urease and invertase, catalase and invertase was positive, but the acid and neutral phosphatase was negatively correlated with other enzymes. Seasonal variation of enzyme activities varied with types of enzyme and fertilizations.

Key words: long-term fertilization , lime concretion black soil , soil enzyme activities

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